8 results about "Dead cell" patented technology

This liquid food contains dead cells of a useful bacterium and a vegetable oil / fat. In the liquid food, the concentration of the dead cells of the useful bacterium is 50 (million / 100 mL) or more, and the concentration of the vegetable oil / fat is 0.1 mass% or more.

The application provides a multifunctional nanofiltration membrane and a preparation method and application thereof, and the preparation method comprises the following steps: first, preparing a hydrophilic polydopamine oxidation layer on a polyamide nanofiltration membrane; then, grafting a PHFBM self-cleaning layer with low surface energy on the polydopamine layer; then, grafting a PMTAC layer with sterilization performance on the PHFBM layer; finally, grafting a zwitterionic polymer PSBMA layer with a defense function on the PMTAC layer, so as to obtain a multifunctional nanofiltration membrane with the functions of defense, resistance and self-cleaning. The multifunctional nanofiltration membrane prepared by the application can effectively improve the pollution phenomenon of microorganisms by the defense of the hydrophilic material and the sterilization effect of the quaternary ammonium salt while maintaining the advantage of high water flux, and the low surface energy material can make dead cells and extracellular polymers automatically fall off from the membrane surface under low shear force, so that the effect of self-cleaning is realized.

The present application relates to the field of biotechnology, in particular to human bladder tissue digestion solution and application thereof.The digestion method of the present application can dissociate human bladder tissue cells while keeping the cell viability at more than 90%, and the amount of harvested cells is also obviously improved.Sufficient cell amount is the prerequisite for optimization treatment such as red blood cell lysis and dead cell removal.The single cell suspension obtained by the method has high viability, low clumping rate, high nucleated rate and less impurities, which can provide a basis for obtaining high-quality gene expression data in human bladder tissue single cell sequencing experiment.The present application can quickly obtain human bladder tissue primary cell suspension (sufficient cell amount, high viability, low clumping rate and less impurities) meeting the requirements of single cell experiment within 2-4 hours.

This invention teaches a tumor-targeting thiocolchicine nanoemulsion wherein the nanodroplets comprising the nanoemulsion are coated with an antinuclear antibody (ANA-nanoemulsion). The nanodroplets are less than 200 nm and will enter the tumor via the Enhanced Permeation and Retention Effect (EPR). Once inside the tumor the antinuclear antibody coating the nanodroplets will bind to extracellular nuclear material released from dead cells and anchor the nanodroplets within the tumor where the drug is released for maximum effect. As all solid tumors have areas of necrosis this means that the ANA-nanoemulsion can target and treat all solid tumors regardless of the tumor type. The ANA-nanoemulsion is formulated to have a phase transition temperature above 8 C. When cooled to below 8 C the ANA-nanoemulsion converts into a suspension of solid lipid nanospheres (ANA-nanospheres) suitable for storage at 4 C or below.

The present application belongs to the field of wastewater biological treatment and microbial electrochemistry technology, and particularly relates to a method for optimizing the ratio of living cells to dead cells and metabolic activity in the electrode surface biofilm by regulating the applied voltage in an inhibitory environment containing toxic substance 5-hydroxymethylfurfural. The present application significantly improves the ratio of living cells in the biofilm and promotes the secretion of functional extracellular polymeric substance by applying a direct current voltage of 0.8-1.4V in an anaerobic electro-fermentation system, thereby enhancing the system's resistance to toxicity. Experiments show that under a voltage of 1.2V, the ratio of living cells in the biofilm can reach more than 85%, the fluorescence intensity of protein-like and humic acid-like substances in the extracellular polymeric substance is the strongest, and the methane production is the highest and the system startup time is the shortest. The present application optimizes the biofilm structure and metabolic function by voltage regulation, thereby providing a systematic solution for the enhanced treatment and resource recovery of inhibitory wastewater.

The present invention relates generally to the concentration of particulate containing samples, such as cells or biomolecules, in order to isolate such particles within a medium and to isolate particle depleted medium. In some embodiments, the present invention relates generally to the separation and / or concentration of cell nuclei from nuclear debris and dead cells.

A method of treatment includes modifying and / or killing cells by changing the cell membrane charging time to make the cells more susceptible to the influence of electric fields. The method may further include inserting one or more electrodes into tissue, acquiring a temperature of the tissue, delivering electrical energy to the tissue by electrical pulses, determining a rate of energy delivery of the electrical energy such that the temperature rises above a first critical temperature but remains below a second critical temperature, and delivering the electrical energy until a specific dose is achieved.

A method for preparing enriched cells is provided. The cells may be from a single donor or from two or more donors. The method includes removing dying or dead cells from primary cells from the donor(s) by a density gradient separation to generate separated cells; cryopreserving the separated cells to generate cryopreserved separated cells; thawing the cryopreserved separated cells to generate thawed separated cells; and removing dying or dead cells from the thawed separated cells by subsequent density gradient separation(s). The primary cells from two or more donors may be combined before or after the first density gradient separation to provide pooled enriched cells. The enriched cells may have a viability rate greater than the primary cells from the donor(s). Also provided is a product including the enriched cells (which may be pooled enriched cells) and methods for using the enriched cells or the product including the enriched cells.