42 results about "Dead cell" patented technology

To provide a container-packed carbonated beverage in which gas leakage is suppressed.SOLUTION: Provided is a packaged carbonated beverage containing dead cells of a useful bacterium, wherein the concentration of the dead cells of the useful bacterium is 500,000,000 cells / L or more, and the gas pressure is 0. 05MPa or more and 0. 5MPa or less.SELECTED DRAWING: None

The invention relates to the field of cell activity detection, and discloses an animal cell activity rapid detection device which comprises a main body, a detection table is arranged at the upper end of the main body, a dustproof glass cover is arranged at the upper end of the detection table, and a rapid mixing assembly is arranged on one side of the upper end of the detection table. The rapid mixing assembly is used for mixing animal cells and trypan blue solution or other auxiliary detection solutions, a detection assembly is arranged on the side, corresponding to the rapid mixing assembly, of the upper end of the detection table, the detection assembly is used for detecting the activity of the animal cells, and an auxiliary detection structure is arranged on the other side, corresponding to the detection assembly, of the upper end of the detection table. According to the invention, the grid pattern design divides the microscopic view into standardized grids, and the AI image analysis is combined to automatically and randomly select a plurality of grids to count the number of live and dead cells, so that the traditional manual counting is replaced, the detection speed is increased, meanwhile, contrast experiment data can be formed, and the activity of animal cells can be rapidly and accurately detected; and human errors are reduced, and animal cell activity data are rapidly obtained.

The present invention provides compositions and methods useful for detecting live cells and dead cells in a cell sample. The methods rely on labelling compounds comprising a maleimide moiety linked to a label.

This liquid food contains dead cells of a useful bacterium and a vegetable oil / fat. In the liquid food, the concentration of the dead cells of the useful bacterium is 50 (million / 100 mL) or more, and the concentration of the vegetable oil / fat is 0.1 mass% or more.

The invention discloses a single-cell suspension purification method capable of maintaining representativeness of a cell population and a kit, and belongs to the technical field of single-cell separation. The method comprises the following steps that a cell sample to be separated and purified is resuspended in a centrifugal container through a reagent B, then a reagent A is added and evenly mixed, the adding amount of the reagent A and the reagent B is adjusted so that the density of the suspension can be 1.08-1.15 g / ml, the reagent A is an aqueous solution containing iodixanol, and the reagent B is a DMEM culture medium containing 1-10% of FBS; and slowly laying PBS on the upper layer of the suspension, centrifuging, collecting living cells of an interface layer, washing the obtained living cells, and re-suspending. According to the scheme, the single-cell suspension for single-cell library construction can be purified under the conditions of low equipment requirement, low cost and mild conditions, the cell population representativeness of a sample can be reserved to the maximum extent while dead cells are removed, and no adverse effect is generated on the single-cell library construction process.

The invention discloses a stem cell-based microvesicle as well as a preparation method and application thereof, belongs to the technical field of biological tissue engineering, and particularly relates to a preparation method of the stem cell-based microvesicle, which comprises the following steps: culturing stem cells until the cell fusion degree is 80-90%, performing hypoxia treatment, and cleaning for 2-3 times; standing and culturing the cleaned stem cells, then adding a calcium ion carrier, and carrying out ultrasonic treatment; the serum-free basic culture medium containing the calcium ion carrier is immediately absorbed and removed, the cells are cleaned through PBS, culture is conducted for 24 h, and cell supernatant is collected and merged respectively; performing first centrifugation to remove cell debris; performing second centrifugation to remove dead cells and large cell debris; carrying out third centrifugation to remove organelles; fourth centrifugation is carried out, large vesicles are removed, and a final supernatant is obtained; and filtering and centrifuging to obtain a microvesicle precipitate. The preparation method of the microvesicles aims at systematically improving the yield and biological activity of the stem cell microvesicles through low-oxygen pre-stimulation, ultrasonic-calcium ion carrier synergistic induction and differential centrifugation.

The application belongs to the technical field of fluorescent probe, and relates to a self-reporting photosensitizer as well as a preparation method and application thereof, and comprises the following steps: 1) under nitrogen protection, IrCl3*3H2O is added into a 2-ethoxyethanol-water solution and mixed; 2-(2,4-difluorophenyl)pyridine is further added, stirred and heated, and then the obtained mixture is subjected to condensation reflux, cooling and vacuum filtration to obtain precipitate; the precipitate is washed and dried to obtain a chloro-bridged dimer; 2) the chloro-bridged dimer is further added into DMSO, and the obtained mixture is subjected to ultrasonic treatment, freeze-drying and separation and purification to obtain the self-reporting photosensitizer. The self-reporting photosensitizer prepared by the application has simple synthesis steps and is easy to operate; the molar absorption coefficient is 6.84*10 4 M ‑1 cm ‑1 , the singlet oxygen yield is 0.45, and the cell phototoxicity index is 15.4; in the application of light-induced cancer cell apoptosis, the fluorescence intensity and fluorescence distribution are observed, and the cell apoptosis is monitored in real time in a double mode, and the living cells and dead cells are obviously distinguished.

The invention belongs to the technical field of microorganisms, and particularly relates to a specific detection method for survival of fusarium pseudograminearum in soil. The method comprises the following steps: adding PMA into a soil sample, carrying out mixed incubation and exposure treatment, extracting DNA, carrying out qPCR detection, preventing DNA amplification of dead cells in total DNA, and determining the number of viable bacteria of fusarium pseudograminearum in the soil. According to the detection method, the optimal PMA concentration is 50 [mu] mol L <-1 >, the optimal exposure time is 15 min, dead and surviving fusarium pseudograminearum spores are distinguished, and the lowest detection limit is 628 spore g <-1 >. The invention provides a rapid, sensitive and reliable method which is used for quantitatively detecting the surviving fusarium pseudograminearum in the soil, and the method has the advantages of high sensitivity, high sensitivity and high sensitivity. Accurate and reliable data are provided for monitoring and early warning, early diagnosis and prevention and control measures of the wheat basal stem rot.

The utility model discloses a stem cell conditioned medium filter device which comprises a filter device shell, a filter tank is arranged in the filter device shell, a square sleeve is arranged on the lower side in the filter tank, a coarse-mesh filter screen is fixedly arranged in the square sleeve, and a filter screen is arranged in the coarse-mesh filter screen. A plurality of fine-hole filter screens fixed in the square sleeve are fixedly arranged below the coarse-hole filter screen; a sealing plate fixed in the square sleeve is arranged above the coarse-mesh filter screen, and a first stepping motor is fixedly mounted in the sealing plate. According to the utility model, the situation that the filter holes in the filter screen are blocked by cell debris, dead cells or metabolic wastes in the stem cell conditioned culture medium and even penetrate through the filter holes due to relatively intense impact generated between the filter screen and the stem cell conditioned culture medium when the filter screen is used for filtering the stem cell conditioned culture medium is prevented; through the technical scheme, the filtering effect of the equipment is improved.

The present invention belongs to the field of genetic engineering technology, and more particularly to a kind of eforRed mutant, its encoding gene and expression vector and its application.The eforRed mutant amino acid sequence is selected from at least one of SEQ ID NO.3-10.The chromogenic protein eforRed mutant provided by the present invention has good visible light excitation activity, and is stronger in fluorescence intensity in the visible light region than the wild-type eforRed and its K201E mutant, and color development is more sensitive and structural stability is also better.In addition, the eforRed mutant of the present invention has no phototoxicity compared to the luciferase reporter gene, does not kill cells, and does not need to add expensive exogenous substrates to develop color. As a reporter gene, it has broad prospects and application advantages in the fields of visual screening of genetic transformation positive transformants, monitoring gene expression activity, protein localization, live cell fluorescence tracing and biological imaging.

The present invention relates generally to systems, materials, and methods utilizing magnetic levitation to affect separation and / or concentration of particulate containing samples, such as cells or biomolecules, within a medium. In some embodiments, the methods are used for the separation of a mixture of live cells and dead cells, and to isolate particle depleted medium.

The invention discloses a bifidobacterium activity dominant population induction culture method and application thereof, and belongs to the technical field of microbial fermention.The induction culture method comprises the steps that a seed solution is inoculated into a culture medium containing lactose, saccharose or maltose to be subjected to anaerobic fermentation to the end of logarithm; after the fermentation liquor is diluted, SYTOTM9 and PI dye are added, and a flow cytometer is used for sorting. According to the method, lactose, cane sugar or maltose is selected as a carbon source for high-density fermentation, living cells, damaged cells and dead cell subgroups are separated in combination with a flow cytometry sorting technology, and a subgroup differentiation mechanism is analyzed by utilizing non-targeted metabonomics, so that the sugar uptake capacity of the living cell subgroups cultured by cane sugar is improved by 30.55% compared with that of the damaged subgroups; the flowability of the membrane is improved by 95.23% compared with that of maltose culture, the thallus activity is remarkably improved, and a theoretical support and a technical method are provided for industrial production of probiotic high-activity preparations.

The invention belongs to the technical field of single-cell metabolome analysis, and particularly relates to a single-cell metabolome analysis method and system with a motility rate sensing function, and the method comprises the steps: obtaining metabolism spectrum data of a plurality of single cells through a single-cell mass spectrum platform, and forming an initial single-cell metabolism matrix; the initial single cell metabolism matrix comprises a plurality of single cell samples and corresponding metabolite ion strength characteristics; inputting single cell survival rate labels provided by an external survival rate judgment module into the analysis process, wherein the survival rate labels are in one-to-one correspondence with corresponding single cells of the initial metabolism matrix; performing subset division on the initial metabolism matrix according to the motility rate label input in the step 2; reconstructing a living cell metabolism matrix and a dead cell metabolism matrix in groups, performing data processing and analysis operation on the living cell metabolism matrix and the dead cell metabolism matrix, and outputting an analysis result with better biological interpretation based on motility rate correction.

The present invention generally relates to the concentration of particle-containing samples, such as cells or biomolecules, in order to separate these particles in a medium and to separate the particle-removed medium. In some embodiments, the present invention generally relates to the isolation and / or concentration of nucleus from nucleus fragments and dead cells.

To provide a dead cell separating agent that enables selective and simple removal of dead cells present in a eukaryotic cell culture liquid, as well as a method for producing eukaryotic cells using the separating agent.SOLUTION: The foregoing problem is solved by the dead cell separating agent comprising an insoluble carrier and Tim4 (T-cell immunoglobulin and mucin domain 4) immobilized on the insoluble carrier.SELECTED DRAWING: None

The present application belongs to the field of marine ecological dynamics and biogeochemical process coupling simulation technology, and particularly relates to a method for regulating marine diatom-microzooplankton competition evolution mechanism by nutrient salt-enzyme activity, comprising the following steps: constructing a model according to the double-algae niche competition principle of nutrient salt and enzyme activity regulation, introducing an enzyme activity regulation submodule, and regulating the growth, metabolism, secretion and death processes of phytoplankton. The model comprises a nutrient salt module, a phytoplankton module and a detritus module, which are respectively used for simulating the migration and transformation process of nutrient salt in the marine environment, the growth and death process of phytoplankton and the transformation process of detritus. The absorption, assimilation and transformation processes of nutrient salt are regulated by enzyme activity, and the detritus is divided into cell detritus and particulate detritus, so as to distinguish the loss of dead cell organic matter and the mineralization process of detritus, and the cell disintegration process is supplemented. Finally, the phytoplankton growth and death dynamics parameters are simulated by the model, and the mechanism of marine diatom-microzooplankton competition evolution is analyzed.

The disclosure relates to compositions and methods for identifying reactive cysteines on nuclear proteins from cell samples. Further wherein the method includes contacting live cells, dead cells, cell lysates, nuclei, or a nuclear fraction obtained from cells with an electrophilic probe, and performing mass spectrometry analysis of proteins present in the cytoplasmic fraction.

The invention discloses a method for detecting a Br element in a single cell based on a mass spectrum flow type. The detection method comprises the following steps: collecting a biological sample exposed by a brominated compound; preparing a single-cell suspension from a biological sample; labeling cells in the single-cell suspension with metal isotopes A and B respectively; the metal isotope A can be used for simultaneously marking survival and dead cells; the metal isotope B can mark dead cells; and performing single cell mass spectrometry flow detection on the single cell suspension, firstly separating single cells according to the signal of the metal isotope A, recording the single cells as a set S, then obtaining individuals with the signal of A but not with the signal of B from the set S, recording the individuals as a set P, and obtaining the single cell mass spectrometry flow detection result through a standard curve according to the detected signal intensity value of the Br element in the cells. According to the method, the Br content in a single cell can be detected, a single-cell inductively coupled plasma time-of-flight mass spectrometer is used for detection, the flux is high, and the analysis speed can reach 600 cells per second.

The present invention relates to a method for labeling or targeting cells whose plasma membrane has lost integrity, such as dead cells, such as for discriminating between live cells and cells whose plasma membrane has lost integrity; to an embodiment of the method for determining one or more values of one or more parameters of cells of a biological sample; to use of the method in an assay for screening drugs for therapy such as cancer therapy; to use of the method to monitor and / or determine the effectiveness of a therapy; to an assay kit; to a complex; to the complex for use as a medicament; to the complex for use in treatment of cancer(s) and / or plaque(s) and / or regeneration and / or supporting the immune system; and, to a dead cell.

The application provides a multifunctional nanofiltration membrane and a preparation method and application thereof, and the preparation method comprises the following steps: first, preparing a hydrophilic polydopamine oxidation layer on a polyamide nanofiltration membrane; then, grafting a PHFBM self-cleaning layer with low surface energy on the polydopamine layer; then, grafting a PMTAC layer with sterilization performance on the PHFBM layer; finally, grafting a zwitterionic polymer PSBMA layer with a defense function on the PMTAC layer, so as to obtain a multifunctional nanofiltration membrane with the functions of defense, resistance and self-cleaning. The multifunctional nanofiltration membrane prepared by the application can effectively improve the pollution phenomenon of microorganisms by the defense of the hydrophilic material and the sterilization effect of the quaternary ammonium salt while maintaining the advantage of high water flux, and the low surface energy material can make dead cells and extracellular polymers automatically fall off from the membrane surface under low shear force, so that the effect of self-cleaning is realized.

The invention relates to a method for removing tissue fragments and impurities in a cell suspension, which comprises the following steps: S1, acquiring a cell suspension, centrifuging the cell suspension, removing supernate, and mixing with a cell precipitation resuspension to obtain a cell resuspension; s2, obtaining a gradient separation liquid and a centrifugal tube, adding the gradient separation liquid into the centrifugal tube, and adding the cell resuspension along the inner wall of the centrifugal tube to form a separation liquid layer and a cell suspension layer in the centrifugal tube; s3, carrying out gradient centrifugation on the centrifugal tube, taking the precipitate, and resuspending to obtain a purified cell suspension; wherein the gradient separation liquid comprises a Percoll solution or a sucrose solution. According to the technical scheme, fragments and impurities are separated by using a gradient density centrifugation method, and living cells, cell fragments and impurity fragments with relatively low density can be separated. And part of dead cells break cell membranes in the centrifugation process, become cell fragments and are removed along with density gradient centrifugation.

The present application relates to the field of biotechnology, in particular to human bladder tissue digestion solution and application thereof.The digestion method of the present application can dissociate human bladder tissue cells while keeping the cell viability at more than 90%, and the amount of harvested cells is also obviously improved.Sufficient cell amount is the prerequisite for optimization treatment such as red blood cell lysis and dead cell removal.The single cell suspension obtained by the method has high viability, low clumping rate, high nucleated rate and less impurities, which can provide a basis for obtaining high-quality gene expression data in human bladder tissue single cell sequencing experiment.The present application can quickly obtain human bladder tissue primary cell suspension (sufficient cell amount, high viability, low clumping rate and less impurities) meeting the requirements of single cell experiment within 2-4 hours.

This invention teaches a tumor-targeting thiocolchicine nanoemulsion wherein the nanodroplets comprising the nanoemulsion are coated with an antinuclear antibody (ANA-nanoemulsion). The nanodroplets are less than 200 nm and will enter the tumor via the Enhanced Permeation and Retention Effect (EPR). Once inside the tumor the antinuclear antibody coating the nanodroplets will bind to extracellular nuclear material released from dead cells and anchor the nanodroplets within the tumor where the drug is released for maximum effect. As all solid tumors have areas of necrosis this means that the ANA-nanoemulsion can target and treat all solid tumors regardless of the tumor type. The ANA-nanoemulsion is formulated to have a phase transition temperature above 8 C. When cooled to below 8 C the ANA-nanoemulsion converts into a suspension of solid lipid nanospheres (ANA-nanospheres) suitable for storage at 4 C or below.

The invention provides a microstructure prediction method and device for an electron beam fuse deposition alloy, belongs to the technical field of electron beam fuse deposition, and solves the technical problem that the alloy microstructure is difficult to predict due to the fact that solidification parameters of a molten pool are difficult to obtain in the deposition process. The method comprises the following steps: constructing a three-dimensional geometric model of the electron beam fuse deposition alloy and carrying out grid division; setting high-temperature thermophysical parameters of the deposition body and the substrate; constructing an electron beam heat source model, boundary conditions and a life and death unit calculation method; solving the transient temperature field distribution in the electron beam fuse deposition alloy molten pool; calculating distribution of temperature gradient and solidification speed in the molten pool; on the basis of the Hunt classical theory, critical structure criteria of alloys with different components are established by utilizing the temperature gradient and the solidification speed; and predicting the microstructure of the alloy in the electron beam molten pool. The method is suitable for predicting the microstructure of the sedimentary body based on the solidification parameters in the electron beam fuse deposition process.

The present invention relates generally to the concentration of particulate containing samples, such as cells or biomolecules, in order to isolate such particles within a medium and to isolate particle depleted medium. In some embodiments, the present invention relates generally to the separation and / or concentration of cell nuclei from nuclear debris and dead cells.

The present application belongs to the field of wastewater biological treatment and microbial electrochemistry technology, and particularly relates to a method for optimizing the ratio of living cells to dead cells and metabolic activity in the electrode surface biofilm by regulating the applied voltage in an inhibitory environment containing toxic substance 5-hydroxymethylfurfural. The present application significantly improves the ratio of living cells in the biofilm and promotes the secretion of functional extracellular polymeric substance by applying a direct current voltage of 0.8-1.4V in an anaerobic electro-fermentation system, thereby enhancing the system's resistance to toxicity. Experiments show that under a voltage of 1.2V, the ratio of living cells in the biofilm can reach more than 85%, the fluorescence intensity of protein-like and humic acid-like substances in the extracellular polymeric substance is the strongest, and the methane production is the highest and the system startup time is the shortest. The present application optimizes the biofilm structure and metabolic function by voltage regulation, thereby providing a systematic solution for the enhanced treatment and resource recovery of inhibitory wastewater.

The invention provides a feeder cell density and motility rate detection kit and a detection method thereof, and belongs to the technical field of cell detection. The invention specifically discloses a method for detecting cell density and / or motility rate, which comprises the following steps: (1) exposing feeder cells to a CFSE dye environment for dyeing; (2) removing the CFSE dye in the environment; (3) culturing the feeder cells under proper conditions; (4) adding absolute counting microspheres into the cultured feeder cells and exposing the feeder cells in a dead cell dye environment for dyeing; and (5) calculating the density or the motility rate of the living cells according to a dyeing result and absolute counting microspheres. The method has good specificity, accuracy, precision and durability, and has good popularization value and market application prospect.

The invention discloses a suspension cell continuous culture process and an impurity removal method of a cell suspension, and belongs to the technical field of cell culture. In the continuous culture process of the suspension cells, dead cells, cell agglomerates and / or cell debris are removed by adjusting the standing and settling conditions, especially the temperature, so that damage to the cells caused by centrifugation, introduction of exogenous reagents and the like is avoided, and the high cell viability is maintained. Dead cells, cell agglomerates and / or cell debris in a cell suspension in a reactor are regularly removed in a continuous culture process, so that single cells with high cell activity and high purity are continuously obtained, rapid and high-value multiplication of the cells is realized, and a guarantee is provided for the stability of subsequent experimental research and industrial production.

The present invention relates generally to the concentration of particulate containing samples, such as cells or biomolecules, in order to isolate such particles within a medium and to isolate particle depleted medium. In some embodiments, the present invention relates generally to the separation and / or concentration of cell nuclei from nuclear debris and dead cells.

The invention discloses a cell culture method, and relates to the technical field of cell culture.The cell culture system comprises a box body, a stirring assembly used for stirring cells and a culture solution is arranged in the box body, a collecting assembly used for assisting observation is arranged in the box body, and a solution changing assembly used for water injection is further arranged in the box body; a matching plate rotates to drive a long swing rod to swing, the long swing rod swings to drive a large gear to rotate around a small gear, a culture solution in the stirring box can be intermittently added into the stirring box through a liquid inlet pipe, a transmission shaft rotates to drive stirring blades to move, and the culture solution and cells in the stirring box can be stirred; the first cam drives the sweeping rod to do telescopic motion in the fixed rod through the pulley, bubbles generated by stirring and dead cells in the culture process can be discharged from the interior of the stirring box through the sweeping rod while the cells and the culture solution are stirred, and the dead cells can be effectively prevented from influencing the cell culture speed.