242 results about "Dead cell" patented technology

In the processes for treating municipal sewage and storm water containing biosolids to discharge standards, biosolids, even after dewatering, contain typically about 80% water bound in the dead cells of the biosolids, which gives biosolids a negative heating value. It can be incinerated only at the expense of purchased fuel. Biosolids are heated to a temperature at which their cell structure is destroyed and, preferably, at which carbon dioxide is split off to lower the oxygen content of the biosolids. The resulting char is not hydrophilic, and it can be efficiently dewatered and / or dried and is a viable renewable fuel. This renewable fuel can be supplemented by also charging conventional biomass (yard and crop waste, etc.) in the same or in parallel facilities. Similarly, non-renewable hydrophilic fuels can be so processed in conjunction with the processing of biosolids to further augment the energy supply.

The invention relates to a preparation method for stem cell secretion factors used for beauty culture and skin protection. The preparation method comprises the following steps: cell separation, stem cell amplification, the acquisition and concentration of a stem cell factor system, and the acquisition of the system containing the stem cell active factors through storage. The system comprises the active factors secreted by various types of currently verified stem cells. The obtained stem cell active factor system neither contains foreign culture solution components nor contains dead cell decomposition components; in addition, the stem cell active factor system can store and maintain the tissue and cell repairing activity for a long period, thereby truly turning the ideal of stem cell beauty culture into a reality, and improving the traditional beauty culture concepts at the biomedical depth. Simultaneously, the preparation method is simple and feasible and is suitable for mass production, thereby providing a shortcut for the stem cells to serve the beauty culture cause.

A methodology of microarray using the fluorescent DNA intercalating agent propidium monoazide (PMA) to selectively block DNA of dead cells from amplification and its application in detecting and enumerating viable microbes in complex microbial communities is described. A phylogenetic array is used in the preferred embodiment to enhance the sensitivity of the method. The PMA-Microarray assay is particularly applicable for monitoring samples from environments with extremely low microbial burden such as spacecraft surfaces.

A method of treating a disease characterized by a pathological immune response in a subject in need thereof is disclosed. The method comprises administering to the subject a therapeutically effective amount of a cell preparation which comprises dying or dead leukocytes, the dying or dead leukocytes being capable of suppressing the pathological immune response, thereby treating the disease in the subject.

A non-destructive method and device for analyzing a sample comprising transparent living and / or dead cells, by 5 means of a digital holographic microscope, where the sample (8) is exposed to light from a laser (2). The light that travels through the cells in the sample will experience a difference in the optical path length compared to the surrounding media and the wave front that emerges from the 10 cells will thus be phase shifted. This distortion can be detected in the digital hologram, which is reconstructed from the interference pattern detected by a digital sensor (17), such as a CCD or a CMOS, as phase differences or phase shifts and thereby creating a digital hologram. The 15 phase shift of each element of the hologram is then used for analyzing the characteristics of the cells in the sample.

Methods and apparatus for the micro-scale, dielectrophoretic separation of particles are provided. Fluid suspensions of particles are sorted and separated by dielectrophoretic separation chambers that have at least two consecutive, electrically coupled planar electrodes separated by a gap in a fluid flow channel. The gap distance as well as applied potential can be used to control the dielectrophoretic forces generated. Using consecutive, electrically coupled electrodes rather than electrically coupled opposing electrodes facilitates higher flow volumes and rates. The methods and apparatus can be used, for example, to sort living, damaged, diseased, and / or dead cells and functionalized or ligand-bound polymer beads for subsequent identification and / or analysis.

The invention provides an article of manufacture comprising a substantially non-immunogenic heart valve xenograft for implantation into humans. The invention further provides methods for preparing a heart valve xenograft by removing at least a portion of a soft tissue from a non-human animal to provide a xenograft; washing the xenograft in saline and alcohol; subjecting the xenograft to cellular disruption treatment; treating the xenograft with crosslinking agents, and digesting the xenograft with a proteoglycan-depleting factor and / or glycosidase. The invention also provides an article of manufacture produced by the above-identified method of the invention. The invention further provides a heart valve xenograft for implantation into a human including a portion of a heart valve from a non-human animal, wherein the portion has extracellular components and substantially only dead cells. The extracellular components have reduced proteoglycan molecules. Each of the xenografts of the invention are substantially non-immunogenic and have substantially the same mechanical properties as a corresponding native heart valve.

A system for detection and control of deposition on pendant tubes in recovery and power boilers includes one or more deposit monitoring sensors operating in infrared regions of about 4 or 8.7 microns and directly producing images of the interior of the boiler, or producing feeding signals to a data processing system for information to enable a distributed control system by which the boilers are operated to operate said boilers more efficiently. The data processing system includes an image pre-processing circuit in which a 2-D image formed by the video data input is captured, and includes a low pass filter for performing noise filtering of said video input. It also includes an image compensation system for array compensation to correct for pixel variation and dead cells, etc., and for correcting geometric distortion. An image segmentation module receives a cleaned image from the image pre-processing circuit for separating the image of the recovery boiler interior into background, pendant tubes, and deposition. It also accomplishes thresholding / clustering on gray scale / texture and makes morphological transforms to smooth regions, and identifies regions by connected components. An image-understanding unit receives a segmented image sent from the image segmentation module and matches derived regions to a 3-D model of said boiler. It derives a 3-D structure the deposition on pendant tubes in the boiler and provides the information about deposits to the plant distributed control system for more efficient operation of the plant pendant tube cleaning and operating systems.

Skin care hand held device and method for caring for human hands and feet useful for safely removing calluses, cuticles, warts and dead cells from human hands and feet, applying a circular, rounded cutting blade to the human skin is disclosed. The present invention relates to a cylindrical scalpel comprising a tube where the tip cut at a 90-degree angle is sharpened to form a curved circular blade integrated with the shank. The blade portion is formed by the inner wall of the tube and the external wall which is formed as to have a curved shape on the outside rim at the tip of the tube, the hollow bore allows access for application of liquid or gaseous additives or vacuum or other types or energy to be applied directly through the tube. Methods for using the foot care device are also disclosed.

Continuously produced algae is seeded into the waste streams by the use of 4 or more photo bio-reactors. The algae so produced manufacture oils and fats inside their cells from nutrients in the waste and the process of photosynthesis. Their growth is enhanced by the use of screens, both fixed and movable. These algae adhere to the screens or to the long chain algae attached there to. Algae removed from the raceway bio-reactor are killed by repeated application of heavy rollers over leveled out extracted algae cells. The dead algae are covered with recycled process waste water which goes to one of more oil / water separators and then to storage tanks that contain bio-diesel oil fraction, to waste water tanks and to storage bins for solid waste dead cell material removed.

The invention discloses a plant enzyme nutrition mask. The mask is prepared from fermentation products of raw materials including, by mass, 5-10 parts of apples, 5-10 parts of cucumbers, 5-10 parts of lemons, 5-10 parts of pineapples, 5-10 parts of citrus peel, 5-10 parts of cladosiphon okamuranus, 5-10 parts of radix sophorae, 5-10 parts of dry roots and rhizomes glycyrrhiza inflata Batal., 5-10 parts of radix scutellariae, 5-10 parts of herb of medicago sativa L., 5-10 parts of aloe and 5-10 parts of beet roots. The plant enzyme nutrition mask can obviously improve the cell functions, repair injured cells in a human body, help the skin to resist oxidation, scavenge free radicals, help the human body skin to remove speckles and eliminate acne and improve the skin elasticity; enzymes have the activating effect on the human body cells and can exclude dead cells and promote healthy cell regeneration division, and therefore the human body health can reach a new level. Compared with a conventional moisture and nutrition supplementing type mask, the plant enzyme nutrition mask can fundamentally solve the problems such as skin premature senility, skin dullness and color spots.

The invention relates to a method for establishing a paclitaxel (PTX)-resistant ovarian cancer cell model. The method comprises the following specific steps: intervening an OVCAR-3 cell and an SK-OV-3 cell by virtue of PTX, replacing a PTX-free culture medium, intervening the OVCAR-3 cell and the SK-OV-3 cell until growth of cells returns to be normal, namely, under the conditions that the cells stably grow, morphologies are full, no floating dead cells are produced and the passage density is 30%, enabling the cells to grow 90% in 3-5 days, starting to carry out normal passage and improving the concentration of PTX, wherein the intervention time for each time is 24 hours; and finally obtaining the PTX-resistant ovarian cancer cell model having the preservation numbers of CCTCC C2014203 and CCTCC C2014204. According to the method, by closely observing growth states of cells and selecting the intervention moment, the established cell model has higher level of drug-resistance coefficients and good resistance stability and can be used for the research on ovarian cancer drug-resistant mechanism, ovarian cancer drug-resistant reversion, prevention of chemotherapy resistance and the improvement on chemosensitivity.

A skin composition which is at least substantially anhydrous and comprisesa) At least one water immiscible emollient oil in sufficient quantity to provide emolliency to skin,b) At least one emulsifying agent capable of forming an emulsion, in situ, on the skin with the above oil when water is added to the said composition, the said emulsifier in such quantities that the oil is substantially removed from the skin,c) A water soluble, at least substantially insoluble in the composition skin exfoliative and / or skin polishing material in sufficient quantities to remove dead cells from the skin during application of the composition and,d) At least one wax in sufficient quantity to at least enhance the physical stabilization of the composition.

In the processes for treating municipal sewage and storm water containing biosolids to discharge standards, biosolids, even after dewatering, contain typically about 80% water bound in the dead cells of the biosolids, which gives biosolids a negative heating value. It can be incinerated only at the expense of purchased fuel.Biosolids are heated to a temperature at which their cell structure is destroyed and, preferably, at which carbon dioxide is split off to lower the oxygen content of the biosolids. The resulting char is not hydrophilic, and it can be efficiently dewatered and / or dried and is a viable renewable fuel. This renewable fuel can be supplemented by also charging conventional biomass (yard and crop waste, etc.) in the same or in parallel facilities. Similarly, non-renewable hydrophilic fuels can be so processed in conjunction with the processing of biosolids to further augment the energy supply.

A system for detection and control of deposition on pendant tubes in recovery and power boilers includes one or more deposit monitoring sensors operating in infrared regions of about 4 or 8.7 microns and directly producing images of the interior of the boiler, or producing feeding signals to a data processing system for information to enable a distributed control system by which the boilers are operated to operate said boilers more efficiently. The data processing system includes an image pre-processing circuit in which a 2-D image formed by the video data input is captured, and includes a low pass filter for performing noise filtering of said video input. It also includes an image compensation system for array compensation to correct for pixel variation and dead cells, etc., and for correcting geometric distortion. An image segmentation module receives a cleaned image from the image pre-processing circuit for separating the image of the recovery boiler interior into background, pendant tubes, and deposition. It also accomplishes thresholding / clustering on gray scale / texture and makes morphological transforms to smooth regions, and identifies regions by connected components. An image-understanding unit receives a segmented image sent from the image segmentation module and matches derived regions to a 3-D model of said boiler. It derives a 3-D structure the deposition on pendant tubes in the boiler and provides the information about deposits to the plant distributed control system for more efficient operation of the plant pendant tube cleaning and operating systems.

The invention relates to a fluorescent staining kit for rapid detection on biological cell viability, and application of the same. The kit comprises an anthocyanin dye and a propidium iodide dye. The detection comprises the following steps of: adding the anthocyanin dye and the propidium iodide in the kit are added in cell suspension, observing and counting the cells by a fluorescence microscope or detecting the cells by a microporous fluorescent plate reader under wavelength of emitted light, and reflecting the viability of the cells in biologic sample liquid according to the strength of fluorescence values of different colors. The kit can be used for rapidly observing or detecting the amount of living cells and the amount of dead cells of various cells in the sample in real time, is visual and handy, eliminates the inference of other substances and the defect of long culture cycle, and is not only suitable for animal cells, but also suitable for most gram negative bacteria and gram positive bacteria.

The invention relates to a freshwater fish demersal egg incubation device and method. The freshwater fish demersal egg incubation device and method are suitable for incubation of fish demersal eggs or adhesive eggs processed in a debonding mode. The device comprises a container, wherein the bottom of the container is in a U shape, one side portion of a container inner cavity is provided with a water injection pipe facing the position below the corresponding side portion of the container, an opening above the other side portion of the container inner cavity is correspondingly provided with an arc turn portion, and the arc turn portion is turned towards one side of the water injection pipe from bottom to top so that injected water flow form annular flow. The method comprises the steps that debonded germ cells are slowly placed in the container filled with water and sink slowly; a water injection pipe valve is opened and is adjusted in a fine mode according to the proportion of the germ cells, the water flow is controlled so that the water flow can rotate in the perpendicular plane in the container to drive the germ cells to rotate, and dead cells and light impurities are removed under the action of centrifugal force; the water flow is kept being injected, and a net bag is cleared when fries are about to be incubated; the fries are collected after being incubated. According to the freshwater fish demersal egg incubation device and method, the germ cells can be prevented from being accumulated on local parts, the dead cells and egg shells are separated from other impurities, and an incubation effect is good.

The invention belongs to the field of xenogenic acellular dermal matrixes and preparation methods thereof. The preparation method of a freeze-thawing xenogenic laser microporous irradiated acellular dermal matrix is characterized by comprising the following steps: chipping a skin graft of piglet skin, and drawing the skin graft; perforating with laser; cleaning by adopting a buffer solution; performing freeze-thawing, and adding the buffer solution which is enough to immerse the skin graft for ultrasonic oscillation so as to remove destroyed dead cells and cell debris to maintain the completeness of structure and base membrane in acellular derma; cleaning with purified water, adding sulfadiazine zinc less than or equal to 0.12mg / cm<2>, and freezing at minus 20 DEG C; and sealing, disinfecting and sterilizing. The freeze-thawing xenogenic laser microporous irradiated acellular dermal matrix prepared with the method is characterized in that: the thickness is between 0.3mm and 0.4mm, the pitch is between 1.0mm and 2.0mm, and the pore diameter is between 500um and 808um.

As an improvement to existing matrix-like power / communications systems, a decentralized array of power and communications components defining a self-configuring modular electrical system which is comprised of components that are completely scalable, easily replaceable, intelligent and combinable in a series, parallel, bypassed state or even capable of elegantly switching in a spare cell(s) to replace a dead cell while interfacing with common battery power chemistry, an external power supply input, a standardized bi-directional data communication input and is combinable and arrangeable into practically any mechanical footprint whereby energy density and communications capability is maximized along with simplicity in accordance with weight and balance considerations and integrated as an autonomous system at the lowest possible cost while being survivable in the harshest of environments including physical shock, vibration, vacuum, radiation, thermal, and electromagnetic interference and providing a communications interface for external control or monitoring via human or other control system input while simultaneously being capable of fully and simply reconfiguring itself if an internal battery cell failure occurs within the system, allowing for instant stabilization to maintain the required uninterrupted power output while providing uninterrupted communications through the system during the upset event while being instantly reconfigurable from series to parallel or vice versa ordering, and being capable of reconfiguring itself autonomously into an arrangement of series / parallel states within its architecture for charge / discharge while enabling cell balancing and continual monitoring all individual cell health status parameters, and only using two wires for all component interconnection.

In the processes for treating municipal sewage and storm water containing biosolids to discharge standards, biosolids, even after dewatering, contain typically about 80% water bound in the dead cells of the biosolids, which gives biosolids a negative heating value. It can be incinerated only at the expense of purchased fuel. Biosolids are heated to a temperature at which their cell structure is destroyed and, preferably, at which carbon dioxide is split off to lower the oxygen content of the biosolids. The resulting char is not hydrophilic, and it can be efficiently dewatered and / or dried and is a viable renewable fuel. This renewable fuel can be supplemented by also charging conventional biomass (yard and crop waste, etc.) in the same or in parallel facilities. Similarly, non-renewable hydrophilic fuels can be so processed in conjunction with the processing of biosolids to further augment the energy supply.

The invention discloses a tea leaf processing method and tea leaves. The tea leaf processing method comprises the following steps: (1) freezing picked fresh leaves under the condition from -10 DEG C to -40 DEG C for more than 3 hours to obtain frozen tea leaves; and (2) withering the frozen tea leaves obtained by the step (1) at a room temperature for 30-80 minutes, and then processing the tea leaves into green tea or black tea. According to the tea leaf processing method disclosed by the invention, tissue cells are crushed by using freezing, and a living cell state before fixation of traditional processing of the tea leaves is changed into a dead cell state; regionalized structures in the cells are damaged by freezing so that the cells lose water, and hydrolytic enzymes (such as nuclease, protease, esterase, glycosidase and pectinase) sufficiently play a role so as to degrade macromolecular substances including polysaccharides, nucleic acid and protein (including polyphenol oxidase) and the like; and the macromolecular substances are easily dissolved out in a tea brewing process, and the utilization rate and the effective quality of nutritional ingredients of the tea leaves are greatly improved.

Fresh tissue slices can be cut from tissue specimens embedded in a low gel point agarose by the use of a vibrating, sliding-blade microtome. The microtome has a blade which vibrates in a generally horizontal direction and which is supported for movement in a generally diagonal, sliding direction with respect to the tissue specimen. The compound vibrating and sliding motion of the cutting blade of the microtome is very effective in cutting fresh tissue slices that have a slice viability, measured as a ratio of live to dead cells, which is much better than that which has been obtained using conventional vibrating microtomes.

The invention discloses a full-automatic cell non-staining image recognizing and counting method. The full-automatic cell non-staining image recognizing and counting method comprises the following steps of: selecting a full-automatic cell counting meter through an image recognition method, adding a cell suspension to be detected into a counting sheet directly, and inserting the cell suspension to be detected into a counting meter; lowering the receiving capability of a light sensing chip to the light intensity of a red light R and a green light G, intensifying the receiving capability of the light sensing chip to the light intensity of a blue light B, and regulating the aeration time and gain manually so as to change the background of a display to be light blue, and take round hot spots as images of active cells, and take the round blue spots darker than the background color as images of dead cells; and analyzing through cell image recognition software, thereby obtaining accurate large-small cell activity analysis data. According to the full-automatic cell non-staining image recognizing and counting method, dyes are not required to be used for counting cell activities; latent toxicity brought to cells to be detected by trypan blue is prevented; detected cells can continue to live, and the activities of the cells are counted during the culturing process; and meanwhile, according to the full-automatic cell non-staining image recognizing and counting method provided by the invention, non-staining real-time on-line cell counting can be achieved, and the method can be widely applied to the field of life science research.

Methods and apparatuses are disclosed for the diagnosis and removal of the devitalized and / or dead cell material formed in the lid margin to attempt to restore a normal lid margin. In this manner, the devitalized and / or dead cell material are removed or the amount present is reduced or no longer present to prevent, reduce, or affect the transport of lipid secreted by the meibomian glands to the tear film to reduce evaporative dry eye and improve dry eye conditions in patients. The diagnosis and removal of devitalized and / or dead cell material may be performed at desired intervals. Patients that suffer from conditions that block meibomian gland orifices, partial, infrequent, or inhibited blinking resulting in reduced lipid secretions from meibomian glands, and / or blockages in meibomian gland channels reducing secretion of lipids through the meibomian gland orifices may require more frequent diagnosis and treatment to remove devitalized and / or dead cell material.

The invention discloses a sample pretreatment method based on flow combination ICP-MS (Inductively Coupled Plasma Mass Spectrometry) single cell protein detection, and belongs to the technical field of sample pretreatment of flow cytometry. The sample pretreatment method comprises the following steps: (1) collecting and transporting a whole blood sample; (2) performing PBMC (Peripheral Blood Mononuclear Cell) cell separation; (3) performing cell active dyeing; (4) performing cell stimulation; (5) performing cell immobilization; (6) performing surface antibody dyeing; (7) performing intracellular phosphorylated protein dyeing; (8) performing single cell labeling, and the like. With the combination of a metal element labeled antibody with cell surface antigen, labeled cells are mixed with beads as internal reference for standardization, dead cells and living cells are distinguished in the pretreatment process, then the situation that the testing result analysis and description are affected by too many dead cells is avoided, single cells are distinguished from cell dimmers, cell trimers or even cell multimers, and flow combination ICP-MS single cell protein detection requirements can be very well met.

A non-destructive method and device for analyzing a sample comprising transparent living and / or dead cells, by 5 means of a digital holographic microscope, where the sample (8) is exposed to light from a laser (2). The light that travels through the cells in the sample will experience a difference in the optical path length compared to the surrounding media and the wave front that emerges from the 10 cells will thus be phase shifted. This distortion can be detected in the digital hologram, which is reconstructed from the interference pattern detected by a digital sensor (17), such as a CCD or a CMOS, as phase differences or phase shifts and thereby creating a digital hologram. The 15 phase shift of each element of the hologram is then used for analyzing the characteristics of the cells in the sample.

The invention discloses a space-dependent type mitochondrial membrane potential fluorescent probe. The probe is a pyridinium salt compound with a structure as shown in the formula (I). Tests show that the probe can mark internal mitochondria in cells with normal mitochondrial membrane potential in a high selectivity manner and can mark the mitochondria and cell nucleuses in the cells with lowered mitochondrial membrane potential, and the larger the lowering degree of the membrane potential is, the higher the fluorescence intensity of the cell nucleuses is; the probe is targeted to the cell nucleuses after the mitochondrial membrane potential disappears completely. In addition, the probe can also distinguish live and dead cells at the same time, the probe is evidently better than currently-used double-dye related products, and the probe is promising in application prospect in terms of biological researches and medical diagnosis.