98 results about "Dead cell" patented technology

A cell sample evaluation apparatus includes an evaluation light irradiation unit for irradiating an irradiation region set in a culture region of a plurality of cells in an object cell sample with evaluation light having a wavelength in a mid-infrared region under a predetermined irradiation condition, a dead cell number measurement unit for measuring a dead cell number generated according to an irradiation amount of the evaluation light in the irradiation region of the object cell sample, and a culture state analysis unit for evaluating a culture state of the object cell sample by obtaining an object correlation between the irradiation amount of the evaluation light and the dead cell number for the object cell sample and comparing a reference correlation between the irradiation amount of the evaluation light and the dead cell number for a reference cell sample and the object correlation.

The invention provides an anti-inflammatory chimera, a reprogramming macrophage and a preparation method and application thereof, and belongs to the technical field of cell therapy. The anti-inflammatory chimera provided by the invention is a chimera based on conversion from an inflammatory signal to an anti-inflammatory signal, and can convert an inflammatory signal of a diseased region of a fibrosis disease into an IL-10 signal, so that the problem of an inflammatory environment of a fibrosis tissue is solved, and steady-state balance of the tissue is promoted. Moreover, the chimera is introduced into the recovery type macrophage, the recovery type phenotype stability of the recovery type macrophage is enhanced, and the purposes of improving the inflammatory environment of the diseased region of the fibrotic disease, reducing fibrotic cell activation, continuously degrading an extracellular matrix, swallowing dead cells and remodeling diseased tissues are achieved. In addition, the invention provides a preparation method of the reprogramming macrophage and a specific application of the reprogramming macrophage in fibrotic tissues.

To provide a container-packed carbonated beverage in which gas leakage is suppressed.SOLUTION: Provided is a packaged carbonated beverage containing dead cells of a useful bacterium, wherein the concentration of the dead cells of the useful bacterium is 500,000,000 cells / L or more, and the gas pressure is 0. 05MPa or more and 0. 5MPa or less.SELECTED DRAWING: None

The invention relates to the field of cell activity detection, and discloses an animal cell activity rapid detection device which comprises a main body, a detection table is arranged at the upper end of the main body, a dustproof glass cover is arranged at the upper end of the detection table, and a rapid mixing assembly is arranged on one side of the upper end of the detection table. The rapid mixing assembly is used for mixing animal cells and trypan blue solution or other auxiliary detection solutions, a detection assembly is arranged on the side, corresponding to the rapid mixing assembly, of the upper end of the detection table, the detection assembly is used for detecting the activity of the animal cells, and an auxiliary detection structure is arranged on the other side, corresponding to the detection assembly, of the upper end of the detection table. According to the invention, the grid pattern design divides the microscopic view into standardized grids, and the AI image analysis is combined to automatically and randomly select a plurality of grids to count the number of live and dead cells, so that the traditional manual counting is replaced, the detection speed is increased, meanwhile, contrast experiment data can be formed, and the activity of animal cells can be rapidly and accurately detected; and human errors are reduced, and animal cell activity data are rapidly obtained.

The invention discloses a method for energizing mesenchymal stem cells, the method can control the cell size, the diameter of each cell is less than 15 [mu] m, the cells can easily pass through blood capillaries, and the risk of vascular embolism is avoided. The survival rate of each part of cells is greater than 98%, dead cells are few, and the energized MSCs have the ability to resist attack of different immune cells, such as macrophages, complements, blood coagulation factors, NK and T cells, high-expression chemotactic factors and blood vessel recognition molecules, good ability to interact with vascular endothelial cells, high-expression anti-apoptosis molecules, and high-expression anti-apoptosis molecules. The influence of adverse factors such as hypoxia, hyperinflammation and high free radicals in a severe microenvironment can be resisted. MSCs subgroups with high expression of stemness genes and high proliferation and cloning capacity are separated and selected, other polluted cells and senescent cells are removed, the MSCs group for amplification is more uniform in form and consistent in function, most of MSCs are controlled, a cell cycle with the consistent length is obtained, and heterogeneity can be reduced.

Provided herein are methods for cell therapy by modifying transfused cells to express an inducible caspase 9 protein, so that the cells may be selectively killed if the patient experiences dangerous side effects. Provided also within relates in part to methods for preventing or treating Graft versus Host Disease by modifying T cells before administration to a patient, so that they may be selectively killed if GvHD develops in the patient.

The present invention provides compositions and methods useful for detecting live cells and dead cells in a cell sample. The methods rely on labelling compounds comprising a maleimide moiety linked to a label.

To provide a microbial cell material having excellent property as an immunostimulation material.SOLUTION: The dead bacterial cells of the genus Brautia are the active ingredient in an immune activator. It is preferable that these dead cells are obtained by heat treatment under acidic conditions. Furthermore, it is preferable that they are obtained by heat treatment under conditions of pH 3.0 to 7.0 and temperature of 70 to 121°C.SELECTED DRAWING: None

This liquid food contains dead cells of a useful bacterium and a vegetable oil / fat. In the liquid food, the concentration of the dead cells of the useful bacterium is 50 (million / 100 mL) or more, and the concentration of the vegetable oil / fat is 0.1 mass% or more.

The invention discloses a single-cell suspension purification method capable of maintaining representativeness of a cell population and a kit, and belongs to the technical field of single-cell separation. The method comprises the following steps that a cell sample to be separated and purified is resuspended in a centrifugal container through a reagent B, then a reagent A is added and evenly mixed, the adding amount of the reagent A and the reagent B is adjusted so that the density of the suspension can be 1.08-1.15 g / ml, the reagent A is an aqueous solution containing iodixanol, and the reagent B is a DMEM culture medium containing 1-10% of FBS; and slowly laying PBS on the upper layer of the suspension, centrifuging, collecting living cells of an interface layer, washing the obtained living cells, and re-suspending. According to the scheme, the single-cell suspension for single-cell library construction can be purified under the conditions of low equipment requirement, low cost and mild conditions, the cell population representativeness of a sample can be reserved to the maximum extent while dead cells are removed, and no adverse effect is generated on the single-cell library construction process.

The invention discloses a stem cell-based microvesicle as well as a preparation method and application thereof, belongs to the technical field of biological tissue engineering, and particularly relates to a preparation method of the stem cell-based microvesicle, which comprises the following steps: culturing stem cells until the cell fusion degree is 80-90%, performing hypoxia treatment, and cleaning for 2-3 times; standing and culturing the cleaned stem cells, then adding a calcium ion carrier, and carrying out ultrasonic treatment; the serum-free basic culture medium containing the calcium ion carrier is immediately absorbed and removed, the cells are cleaned through PBS, culture is conducted for 24 h, and cell supernatant is collected and merged respectively; performing first centrifugation to remove cell debris; performing second centrifugation to remove dead cells and large cell debris; carrying out third centrifugation to remove organelles; fourth centrifugation is carried out, large vesicles are removed, and a final supernatant is obtained; and filtering and centrifuging to obtain a microvesicle precipitate. The preparation method of the microvesicles aims at systematically improving the yield and biological activity of the stem cell microvesicles through low-oxygen pre-stimulation, ultrasonic-calcium ion carrier synergistic induction and differential centrifugation.

The application belongs to the technical field of fluorescent probe, and relates to a self-reporting photosensitizer as well as a preparation method and application thereof, and comprises the following steps: 1) under nitrogen protection, IrCl3*3H2O is added into a 2-ethoxyethanol-water solution and mixed; 2-(2,4-difluorophenyl)pyridine is further added, stirred and heated, and then the obtained mixture is subjected to condensation reflux, cooling and vacuum filtration to obtain precipitate; the precipitate is washed and dried to obtain a chloro-bridged dimer; 2) the chloro-bridged dimer is further added into DMSO, and the obtained mixture is subjected to ultrasonic treatment, freeze-drying and separation and purification to obtain the self-reporting photosensitizer. The self-reporting photosensitizer prepared by the application has simple synthesis steps and is easy to operate; the molar absorption coefficient is 6.84*10 4 M ‑1 cm ‑1 , the singlet oxygen yield is 0.45, and the cell phototoxicity index is 15.4; in the application of light-induced cancer cell apoptosis, the fluorescence intensity and fluorescence distribution are observed, and the cell apoptosis is monitored in real time in a double mode, and the living cells and dead cells are obviously distinguished.

The invention belongs to the technical field of microorganisms, and particularly relates to a specific detection method for survival of fusarium pseudograminearum in soil. The method comprises the following steps: adding PMA into a soil sample, carrying out mixed incubation and exposure treatment, extracting DNA, carrying out qPCR detection, preventing DNA amplification of dead cells in total DNA, and determining the number of viable bacteria of fusarium pseudograminearum in the soil. According to the detection method, the optimal PMA concentration is 50 [mu] mol L <-1 >, the optimal exposure time is 15 min, dead and surviving fusarium pseudograminearum spores are distinguished, and the lowest detection limit is 628 spore g <-1 >. The invention provides a rapid, sensitive and reliable method which is used for quantitatively detecting the surviving fusarium pseudograminearum in the soil, and the method has the advantages of high sensitivity, high sensitivity and high sensitivity. Accurate and reliable data are provided for monitoring and early warning, early diagnosis and prevention and control measures of the wheat basal stem rot.

The utility model discloses a stem cell conditioned medium filter device which comprises a filter device shell, a filter tank is arranged in the filter device shell, a square sleeve is arranged on the lower side in the filter tank, a coarse-mesh filter screen is fixedly arranged in the square sleeve, and a filter screen is arranged in the coarse-mesh filter screen. A plurality of fine-hole filter screens fixed in the square sleeve are fixedly arranged below the coarse-hole filter screen; a sealing plate fixed in the square sleeve is arranged above the coarse-mesh filter screen, and a first stepping motor is fixedly mounted in the sealing plate. According to the utility model, the situation that the filter holes in the filter screen are blocked by cell debris, dead cells or metabolic wastes in the stem cell conditioned culture medium and even penetrate through the filter holes due to relatively intense impact generated between the filter screen and the stem cell conditioned culture medium when the filter screen is used for filtering the stem cell conditioned culture medium is prevented; through the technical scheme, the filtering effect of the equipment is improved.

The present invention belongs to the field of genetic engineering technology, and more particularly to a kind of eforRed mutant, its encoding gene and expression vector and its application.The eforRed mutant amino acid sequence is selected from at least one of SEQ ID NO.3-10.The chromogenic protein eforRed mutant provided by the present invention has good visible light excitation activity, and is stronger in fluorescence intensity in the visible light region than the wild-type eforRed and its K201E mutant, and color development is more sensitive and structural stability is also better.In addition, the eforRed mutant of the present invention has no phototoxicity compared to the luciferase reporter gene, does not kill cells, and does not need to add expensive exogenous substrates to develop color. As a reporter gene, it has broad prospects and application advantages in the fields of visual screening of genetic transformation positive transformants, monitoring gene expression activity, protein localization, live cell fluorescence tracing and biological imaging.

The present invention relates generally to systems, materials, and methods utilizing magnetic levitation to affect separation and / or concentration of particulate containing samples, such as cells or biomolecules, within a medium. In some embodiments, the methods are used for the separation of a mixture of live cells and dead cells, and to isolate particle depleted medium.

The invention discloses a bifidobacterium activity dominant population induction culture method and application thereof, and belongs to the technical field of microbial fermention.The induction culture method comprises the steps that a seed solution is inoculated into a culture medium containing lactose, saccharose or maltose to be subjected to anaerobic fermentation to the end of logarithm; after the fermentation liquor is diluted, SYTOTM9 and PI dye are added, and a flow cytometer is used for sorting. According to the method, lactose, cane sugar or maltose is selected as a carbon source for high-density fermentation, living cells, damaged cells and dead cell subgroups are separated in combination with a flow cytometry sorting technology, and a subgroup differentiation mechanism is analyzed by utilizing non-targeted metabonomics, so that the sugar uptake capacity of the living cell subgroups cultured by cane sugar is improved by 30.55% compared with that of the damaged subgroups; the flowability of the membrane is improved by 95.23% compared with that of maltose culture, the thallus activity is remarkably improved, and a theoretical support and a technical method are provided for industrial production of probiotic high-activity preparations.

The invention belongs to the technical field of single-cell metabolome analysis, and particularly relates to a single-cell metabolome analysis method and system with a motility rate sensing function, and the method comprises the steps: obtaining metabolism spectrum data of a plurality of single cells through a single-cell mass spectrum platform, and forming an initial single-cell metabolism matrix; the initial single cell metabolism matrix comprises a plurality of single cell samples and corresponding metabolite ion strength characteristics; inputting single cell survival rate labels provided by an external survival rate judgment module into the analysis process, wherein the survival rate labels are in one-to-one correspondence with corresponding single cells of the initial metabolism matrix; performing subset division on the initial metabolism matrix according to the motility rate label input in the step 2; reconstructing a living cell metabolism matrix and a dead cell metabolism matrix in groups, performing data processing and analysis operation on the living cell metabolism matrix and the dead cell metabolism matrix, and outputting an analysis result with better biological interpretation based on motility rate correction.

The present disclosure relates to a hydrocolloid sheet having an exfoliating function, comprising: a release layer; a hydrocolloid layer which is stacked on the upper surface of the release layer and contains an adhesive, an absorbent, and an exfoliating agent; and a base layer which is stacked on the upper surface of the hydrocolloid layer and protects the hydrocolloid layer. The hydrocolloid sheet having an exfoliating function, according to the present disclosure, exerts an effect of removing unnecessary dead cells on the skin surface and in pores, simply by being attached to skin.

The present invention generally relates to the concentration of particle-containing samples, such as cells or biomolecules, in order to separate these particles in a medium and to separate the particle-removed medium. In some embodiments, the present invention generally relates to the isolation and / or concentration of nucleus from nucleus fragments and dead cells.

The invention discloses an MDS-AML azacitidine drug-resistant strain, and belongs to the field of drug-resistant strains, the drug-resistant strain is preserved in the China Center for Type Culture Collection, the preservation name is SKM / Aza, and the preservation number is C2024248; the invention also discloses a construction method of the MDS-to-AML azacitidine drug-resistant strain, and the construction method is characterized by comprising the following steps: S1, subculturing an initial SKM1 cell in a cell culture device; s2, adding azacitidine with the drug concentration of 0.1 mu M into the cell culture device, treating for 24 hours, centrifuging at a low speed, changing the liquid and removing the drug to remove dead cells, treating by using the drug with the concentration of 0.1 mu M after the cell state is recovered, observing that the number of dead cells is large, namely removing the drug to recover, and repeating the operation of adding the drug and removing the drug until the cells stably grow under the concentration, so as to obtain the azacitidine. Entering the next stage of drug concentration treatment; and S3, according to the drug treatment concentration, selecting a specific drug concentration for progressive increase treatment.

To provide a dead cell separating agent that enables selective and simple removal of dead cells present in a eukaryotic cell culture liquid, as well as a method for producing eukaryotic cells using the separating agent.SOLUTION: The foregoing problem is solved by the dead cell separating agent comprising an insoluble carrier and Tim4 (T-cell immunoglobulin and mucin domain 4) immobilized on the insoluble carrier.SELECTED DRAWING: None

To provide a technique enabling the analysis of cell viability information and nucleic acid information together for each single cell in a cell population when analyzing the composition of the cell population.SOLUTION: The present disclosure discloses a method that is for analyzing the composition of a cell population and that comprises: a step for providing gel capsules in each of which a single cell of the cell population is encapsulated in a state of being labeled with a labeling agent, where the labeling agent distinctively labels a living cell and a dead cell; a step for amplifying a nucleic acid derived from each single cell of the cell population; a step for obtaining cell viability information for each single cell of the cell population; and a step for analyzing the amplified nucleic acid derived from each single cell of the cell population.SELECTED DRAWING: None

The present application belongs to the field of marine ecological dynamics and biogeochemical process coupling simulation technology, and particularly relates to a method for regulating marine diatom-microzooplankton competition evolution mechanism by nutrient salt-enzyme activity, comprising the following steps: constructing a model according to the double-algae niche competition principle of nutrient salt and enzyme activity regulation, introducing an enzyme activity regulation submodule, and regulating the growth, metabolism, secretion and death processes of phytoplankton. The model comprises a nutrient salt module, a phytoplankton module and a detritus module, which are respectively used for simulating the migration and transformation process of nutrient salt in the marine environment, the growth and death process of phytoplankton and the transformation process of detritus. The absorption, assimilation and transformation processes of nutrient salt are regulated by enzyme activity, and the detritus is divided into cell detritus and particulate detritus, so as to distinguish the loss of dead cell organic matter and the mineralization process of detritus, and the cell disintegration process is supplemented. Finally, the phytoplankton growth and death dynamics parameters are simulated by the model, and the mechanism of marine diatom-microzooplankton competition evolution is analyzed.

To provide a microbial cell material having excellent property.SOLUTION: This is a dead cell body of bacteria from the Blautia genus, characterized by being obtained by heat treatment of Blautia genus bacteria under acidic conditions. Additionally, it is a dead cell body of Blautia genus bacteria, where the IL-12 production induction capability measured using mouse spleen cells is more than ten times greater compared to the IL-12 production induction capability by live Blautia genus bacteria under the same conditions. It also refers to a method for producing the dead cell body of Blautia genus bacteria, characterized by subjecting live Blautia genus bacteria to heat treatment under acidic conditions.SELECTED DRAWING: None

To provide a food component having high protein content, and a food or a beverage including the food component.SOLUTION: There is provided a food component containing cells of at least one bacterium, the cells including a crude protein concentration of at least 60 wt.% of a total dry weight of the cells and nucleic acid at a concentration of less than about 5 wt.% of the total dry weight of the cells, where at least 50% of a total number of the cells are dead cells. Further provided are a food and a beverage including the food component and a production method thereof.SELECTED DRAWING: None

A cell sample measurement device (1A) is provided with: a sample placement table (18) on which a target cell sample (S) containing a plurality of cells cultured on a culture dish (13) is placed; an evaluation light irradiation unit (20) that irradiates evaluation light having a mid-infrared region wavelength from an irradiation light source (21) at different irradiation amounts to each of N irradiation regions set by dividing the culture region in the target cell sample (S); a dead cell count measurement unit (30) that measures the number of dead cells generated on the basis of the amount of irradiation of the evaluation light for each of the N irradiation regions of the target cell sample (S); and a culture state analysis unit (50) that obtains, for the target cell sample (S), a target correlation between the number of dead cells and the irradiation amount of evaluation light in N irradiation regions that are indexes for evaluating the culture state of the target cell sample (S). As a result, it is possible to appropriately measure the culture state of a cell sample containing a plurality of cells and to achieve a cell sample measurement method and a cell sample measurement device.

The disclosure relates to compositions and methods for identifying reactive cysteines on nuclear proteins from cell samples. Further wherein the method includes contacting live cells, dead cells, cell lysates, nuclei, or a nuclear fraction obtained from cells with an electrophilic probe, and performing mass spectrometry analysis of proteins present in the cytoplasmic fraction.

The invention discloses a method for detecting a Br element in a single cell based on a mass spectrum flow type. The detection method comprises the following steps: collecting a biological sample exposed by a brominated compound; preparing a single-cell suspension from a biological sample; labeling cells in the single-cell suspension with metal isotopes A and B respectively; the metal isotope A can be used for simultaneously marking survival and dead cells; the metal isotope B can mark dead cells; and performing single cell mass spectrometry flow detection on the single cell suspension, firstly separating single cells according to the signal of the metal isotope A, recording the single cells as a set S, then obtaining individuals with the signal of A but not with the signal of B from the set S, recording the individuals as a set P, and obtaining the single cell mass spectrometry flow detection result through a standard curve according to the detected signal intensity value of the Br element in the cells. According to the method, the Br content in a single cell can be detected, a single-cell inductively coupled plasma time-of-flight mass spectrometer is used for detection, the flux is high, and the analysis speed can reach 600 cells per second.

The present invention relates to a method for labeling or targeting cells whose plasma membrane has lost integrity, such as dead cells, such as for discriminating between live cells and cells whose plasma membrane has lost integrity; to an embodiment of the method for determining one or more values of one or more parameters of cells of a biological sample; to use of the method in an assay for screening drugs for therapy such as cancer therapy; to use of the method to monitor and / or determine the effectiveness of a therapy; to an assay kit; to a complex; to the complex for use as a medicament; to the complex for use in treatment of cancer(s) and / or plaque(s) and / or regeneration and / or supporting the immune system; and, to a dead cell.