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Method for preparing stem cell secretion factor for beauty treatment and skin-protection

A stem cell factor and secretory factor technology, applied in skin care preparations, cosmetic preparations, pharmaceutical formulations, etc., can solve problems such as easy to cause dependence, and achieve the effect of promoting exchange, promoting skin metabolism and enhancing vitality

Inactive Publication Date: 2009-06-24
天津欧瑞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, growth factors such as bFGF and EGF are added to beauty and skin care products on the market, and cell renewal is a systematic process. Although the addition of a single growth factor can stimulate cell metabolism to a certain extent, it is also easy to cause dependence. sex

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Mouse embryonic stem cells: from Nankai University Biology Center

[0021] Culture and expansion of ES cells: take 1ml of embryonic stem cells (ES), and the cell concentration is about 1×10 6 Cells / ml, pipette several times with a syringe or a pipette to ensure a single-cell suspension, and spread the cells in culture flasks with primary mouse embryonic fibroblasts (MEFs), ES maintenance medium (Tianjin Lianxing Biological Co., Ltd. Technology Co., Ltd.) 10ml culture, replace the culture medium after overnight culture, digest, subculture after 2-3 days, and culture again. Repeat the above operations to obtain a sufficient number of ESs.

[0022] Preparation of stem cell activity factors: when ES cells grow most vigorously (approximately 70-80% of the area of ​​the culture bottle), discard the medium, wash with PBS (or saline) for 2-3 times, then add 10ml of PBS, and continue to cultivate 24 hours. The supernatant was taken and centrifuged to obtain a stem cell factor...

Embodiment 2

[0026] This implementation example provides the preparation process of the stem cell factor system derived from bone marrow, as follows:

[0027] 1. Acquisition of primary bone marrow stem cells: take 10ml of healthy human bone marrow, dilute it to 20ml with PBS, and obtain bone marrow mononuclear cells through Ficoll density gradient centrifugation. According to the characteristics that MSCs tend to adhere to the surface of polystyrene, MSCs and Hematopoietic cell isolation. Small colonies of cells began to appear after 3 days of culture. After about 7-9 days, the cells reached saturation, at which time the cells were subcultured. After 3 hours of subculture, the cells were completely attached to the wall. After 2 days, the cells showed a typical fibroblast-like morphology and were evenly distributed in the culture flask. After 3-4 days, the cell density reaches 80-90%.

[0028] 2. Cell expansion: when the cell density reaches 80-90%, gently wash the cells in the culture f...

Embodiment 3

[0032] This embodiment provides the preparation process of the epidermal stem cell active factor system, specifically as follows:

[0033] Cell source: from healthy human skin (or newborn foreskin).

[0034] Cell isolation and culture method: culture according to conventional methods, for details, refer to (see "Cultivated Cytology and Cell Culture Technology" edited by Zhang Zhuoran).

[0035] Preparation of stem cell activity factors: when epidermal stem cells grow most vigorously (about 70-80% of the area of ​​the culture bottle), discard the medium, wash with PBS or saline for 2-3 times, then add 10ml of PBS, and continue to cultivate for 24 hours. The supernatant was taken and centrifuged to obtain a supernatant rich in stem cell factor. Digest the cells and count the cells, according to 1ml supernatant / 1×10 6 Concentrate the supernatant at the concentration of 1 cell.

[0036] Preservation of stem cell compound active factors: put the above concentrated supernatants (...

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Abstract

The invention relates to a preparation method for stem cell secretion factors used for beauty culture and skin protection. The preparation method comprises the following steps: cell separation, stem cell amplification, the acquisition and concentration of a stem cell factor system, and the acquisition of the system containing the stem cell active factors through storage. The system comprises the active factors secreted by various types of currently verified stem cells. The obtained stem cell active factor system neither contains foreign culture solution components nor contains dead cell decomposition components; in addition, the stem cell active factor system can store and maintain the tissue and cell repairing activity for a long period, thereby truly turning the ideal of stem cell beauty culture into a reality, and improving the traditional beauty culture concepts at the biomedical depth. Simultaneously, the preparation method is simple and feasible and is suitable for mass production, thereby providing a shortcut for the stem cells to serve the beauty culture cause.

Description

technical field [0001] The invention relates to a preparation method of stem cell secreted factor which can be used for cosmetic and skin care. The compound factors secreted by the stem cells can be applied to beauty cosmetics, health care products and skin care products. Background technique [0002] Stem cells are a kind of cells with multi-directional differentiation potential, which can be divided into embryonic stem cells and adult stem cells according to different sources. Embryonic stem cells can differentiate into any type of cell in the body, so they are also called totipotent stem cells. Adult stem cells refer to those stem cells with tissue or organ specificity, which can differentiate into cell lines suitable for their location in a suitable environment, also known as pluripotent stem cells. Stem cells can be directed to differentiate in a specific environment, so as to repair tissue and cell damage. The inventor's research shows that (Bc1-2 engineered MSCs in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61K8/65A61Q19/00
Inventor 欧来良李文忠
Owner 天津欧瑞生物科技有限公司
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