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Preparation method for concentrating stem cell paracrine factor and removing invalid impurity protein

A technology of stem cell factors and stem cells, which is applied in the field of preparation of stem cell secreted factors, can solve problems such as allergies and dilution of active ingredients, and achieve the effect of avoiding damage

Inactive Publication Date: 2020-09-11
卡杜兰(广州)生命基因工程科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the culture medium contains a large number of cell metabolites, cell membrane fragments, and many invalid proteins during preparation.
Allergenic and dilute active ingredients

Method used

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Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0015] The present invention will be further described below. It should be noted that the following examples are based on the technical solution and provide detailed implementation and specific operation process, but the protection scope of the present invention is not limited to this example.

[0016] The invention is a preparation method for concentrating stem cell paracrine factors and removing invalid impurity proteins, and the method includes the following steps:

[0017] S1 Separation of stem cells;

[0018] S2 amplifying the stem cells obtained in step S1;

[0019] For the acquisition of S3 stem cell factor system, during the most vigorous period of stem cell division, discard the normal medium, wash with PBS buffer (phosphate buffer, pH=7.4 or so) for 1-3 times, then add PBS or normal saline, and culture the cells Cultivate in the box for 4-24 hours, collect the supernatant, and then obtain a solution rich in stem cell growth factor system;

[0020] S4 Concentration ...

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Abstract

The invention discloses a preparation method for concentrating a stem cell paracrine factor and removing invalid impurity protein. The method comprises the following steps: separating stem cells; amplifying the obtained stem cells; obtaining a stem cell factor system, namely removing a normal culture medium in the most vigorous division period of the stem cells, cleaning for 1-3 times by using a PBS buffer solution (phosphate buffer solution, pH is about 7.4), adding PBS or normal saline, culturing for 4-24 hours in a cell incubator, and collecting the supernatant, thereby obtaining a solutionrich in a stem cell growth factor system; and concentrating and storing, namely centrifuging the obtained liquid, removing scattered cells, concentrating according to the concentration of 1*106 cells / ml, thereby obtaining the product. The product can be stored at the temperature of 20 DEG C for a long time for later use. The stem cell paracrine factor obtained by the method does not contain any external culture solution component or dead cell lysis component. Moreover, the tissue and cell repair activity can be maintained due to long-term storage.

Description

technical field [0001] The invention relates to a preparation method of stem cell secreted factor which can be used for cosmetic and skin care. , especially relates to a preparation method for concentrating stem cell paracrine factors and removing invalid impurity proteins. Background technique [0002] Stem cells are a kind of cells with multi-directional differentiation potential, which can be divided into embryonic stem cells and adult stem cells according to different sources. Embryonic stem cells can differentiate into any type of cell in the body, so they are also called totipotent stem cells. Adult stem cells refer to those stem cells with tissue or organ specificity, which can differentiate into cell lines suitable for their location in a suitable environment, also known as pluripotent stem cells. Stem cells can be directed to differentiate in a specific environment, so as to repair tissue and cell damage. [0003] However, the culture medium contains a large amou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64A61K8/99A61Q19/00A61P17/02
CPCA61K8/64A61K8/99A61Q19/00A61P17/02A61K2800/805
Inventor 朱瑜
Owner 卡杜兰(广州)生命基因工程科技有限公司
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