Full-automatic cell non-staining image recognizing and counting method

A technology of image recognition and counting methods, applied in the field of detection, can solve the problems of culture and proliferation, embryonic cells, and the inability to judge the activity and quantity of endocytic cells

Active Publication Date: 2013-06-12
广州博大博聚科技有限公司
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Problems solved by technology

[0007] In order to solve the problems of the traditional cell counting method of image recognition after staining, the toxicity to living cells, the inability to count online, and the inability to stain embryonic cells For subsequent culture and proliferation and the inability to judge the activity and quantity of cells with endocytic function, the purpose of the present invention is to pr

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  • Full-automatic cell non-staining image recognizing and counting method
  • Full-automatic cell non-staining image recognizing and counting method
  • Full-automatic cell non-staining image recognizing and counting method

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Embodiment 1

[0031] Depend on figure 1 It can be seen that: the present invention selects the image recognition method full-automatic cell counter, and the counter structure includes a point light source 1, a convex lens 2, a counting sheet 3, a focusing objective lens 4, an optical filter 5, a photosensitive chip 6, a data line 7, Controller 8, image recognition software 9, display 10; Its operating steps are as follows:

[0032] First, use a pipette to take 10ul of the cell suspension to be tested, and directly add the non-stained cell suspension to the 3-counting slide and insert it into the counter;

[0033] Depend on figure 2 It can be seen that the parameter setting of the photosensitive chip 6 has been adjusted to the optimal value and set as the default value;

[0034] Do white balance first, record all parameter values ​​after white balance: including red light R gain, green light G gain, blue light B gain, gamma value, contrast, automatic color gain, automatic exposure value,...

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Abstract

The invention discloses a full-automatic cell non-staining image recognizing and counting method. The full-automatic cell non-staining image recognizing and counting method comprises the following steps of: selecting a full-automatic cell counting meter through an image recognition method, adding a cell suspension to be detected into a counting sheet directly, and inserting the cell suspension to be detected into a counting meter; lowering the receiving capability of a light sensing chip to the light intensity of a red light R and a green light G, intensifying the receiving capability of the light sensing chip to the light intensity of a blue light B, and regulating the aeration time and gain manually so as to change the background of a display to be light blue, and take round hot spots as images of active cells, and take the round blue spots darker than the background color as images of dead cells; and analyzing through cell image recognition software, thereby obtaining accurate large-small cell activity analysis data. According to the full-automatic cell non-staining image recognizing and counting method, dyes are not required to be used for counting cell activities; latent toxicity brought to cells to be detected by trypan blue is prevented; detected cells can continue to live, and the activities of the cells are counted during the culturing process; and meanwhile, according to the full-automatic cell non-staining image recognizing and counting method provided by the invention, non-staining real-time on-line cell counting can be achieved, and the method can be widely applied to the field of life science research.

Description

technical field [0001] The invention relates to a detection method, in particular to a fully automatic cell non-staining image recognition and counting method for detecting cell quantity and activity. Background technique [0002] Since the cells must maintain a certain concentration in order to have better activity during culture, the cells need to be counted several times according to the actual situation in the process of cell recovery, culture, and cryopreservation; at present, the commonly used cell counting methods include automatic image recognition method and electrical impedance counting method. [0003] The electrical impedance counting method is based on the detection of the resistance change caused by the cells suspended in the electrolyte solution when they pass through the counting channel, and the cell count and volume are determined. This method is called the electrical impedance method, also known as the Coulter principle. . [0004] Disadvantages of the e...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
Inventor 朱耀辉余海滨赵光
Owner 广州博大博聚科技有限公司
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