33results about How to "Avoid Potential Toxicity" patented technology

The present invention provides methods for the conditional or regulated expression of a site-specific recombinase using split intein-mediated protein splicing. This enhances the temporal and tissue-specificity of trait gene expression and allows for fine tuning of expression specificity.

The present invention relates to stable, preservative- and antioxidant-free liquid formulations of phenylephrine and ketorolac for injection.

The invention relates to a new drug combination for treating diseases of traumatology, a preparation method and application of the drug combination, a medicinal preparation containing the drug combination and quality control method thereof. The drug combination provided by the invention can quickly and durably relieve pains, obviously and rapidly alleviate edema, facilitate callus growth and accelerate fracture healing; compared with the drug combination prepared by the prior art, the new drug combination has better curative effect and high safety.

The invention provides a polypeptide for preparing hydrogel. The amino acid sequence of the polypeptide is SEQ ID NO: 1. The polypeptide is used for preparing the hydrogel. The prepared hydrogel can be used for producing cell culture holders. The polypeptide has the advantages that enzymes of organisms are catalyzed under physiological conditions to form the hydrogel, and accordingly, damage of external chemical agents, ultraviolet light and the like to tissues is avoided; the biological enzymes can be degraded, the hydrogel is degraded by endogenous substances in the organisms, and accordingly, potential toxicity and immunogenicity risks caused by introduction of exogenous chemical agents are avoided; the polypeptide is an ideal tissue engineering material and is significant to human life health.

The invention provides a preparation method and application of carrier-free double-drug self-assembled nanoparticles. A chemotherapeutic drug sorafenib and a natural product ursolic acid molecule are self-assembled by a solvent exchange method through a pi-alkyl bond, an alkyl bond and a hydrogen bond to form nanoparticles, then indocyanine green is physically adsorbed into the nanoparticles, and the surfaces of the nanoparticles are modified with nucleic acid aptamers and cell-penetrating peptides, so that the indocyanine green nanoparticles are prepared. Thus, the carrier-free double-drug self-assembled nanoparticles are prepared. Potential toxicity caused by introduction and co-modification of a traditional polymer or inorganic carrier is effectively avoided, and a new way is provided for application of a nanotechnology to the field of cancer treatment.

The invention discloses a method for preparing a DNA nano preparation for photo-thermal-immune combined treatment of tumors. The method mainly comprises the following steps: (1) a DNA-A and nucleic acid aptamer polymer chain is prepared; (2) a CPG-ODN polymer chain is prepared; (3) the DNA-A polymer chain and the CPG-ODN polymer chain are complementary with a Linker DNA base, and the chains and the Linker DNA base can be crosslinked to form DNA microgel nanoparticles, and meanwhile, an indocyanine green (ICG) photo-thermal agent is added, and ICG-coated microgel particles are formed through crosslinking; and (4) loading of a chemotherapeutic drug DOX is carried out. A nucleic acid aptamer is mixed in a DNA-A solution to form a DNA-A polymer chain with a nucleic acid aptamer. The microgel nanoparticles combine chemotherapy and immunotherapy, so that the tumor treatment effect is greatly enhanced, and tumor treatment is more thorough.

The present disclosure relates to an antimicrobial peptide conjugated with nanoparticles, in particular the antimicrobial peptide LL37-conjugated gold nanoparticles for use in medicine or cosmetic, for human or animals, namely in the treatment of wound healing or in the treatment of ischemic or vascular diseases, comprising a gold nanoparticle and a plurality of LL-37 peptides, wherein the plurality of LL-37 peptides is bound to the gold nanoparticle surface.

The invention relates to a novel medicine composition for treating traumatology diseases, a preparation method of the medicine composition, application, a medicine preparation comprising the medicine composition and a quality control method of the medicine preparation. The medicine composition has functions of rapidly and lastingly relieving pain, obviously and fast subsiding swelling, promoting growth of callus and accelerating fracture healing, and has a better curative effect and higher safety as compared with a medicine composition in the prior art.

The invention provides a preparation method of fucoidin self-assembled drug-loaded nanoparticles and application of the fucoidin self-assembled drug-loaded nanoparticles in prevention of renal injury. The preparation method comprises the following steps: connecting an indissolvable hydrophobic drug to fucoidin through a chemical bond, and then entrapping the hydrophobic drug through self-assembly to obtain the self-assembled nanoparticles, and specifically comprises the following steps: adding fucoidin powder into a proper amount of deionized water, carrying out ultrasonic-assisted dissolution and stirring to obtain a fucoidin solution with the concentration of 10 mg/ml; the preparation method comprises the following steps: dissolving a hydrophobic drug in a trace amount of ethanol (the volume ratio of ethanol to water is 1: 10-1: 20), dropwise adding the hydrophobic drug into a fucoidin solution by using an anti-solvent method to prepare self-assembled nanoparticles, and stirring and volatilizing ethanol by using a solvent volatilization method to obtain the nanoparticles with the pH value of 6-7, the particle size of about 150nm and the maximum drug loading capacity of 60%. According to the invention, fucoidin with good biocompatibility is used as a transport carrier, and the self-assembled nanoparticles containing hydrophobic drugs such as ferulic acid, caffeic acid and high polymer procyanidine are obtained. The carrier material selected by the method has functionality, so that the drug stability can be improved, and the dosage and toxicity can be reduced. The method is simple to operate, simple in equipment and suitable for industrial production, and has good social value and application prospect.

The invention discloses a nanocomposite hydrogel dressing and its preparation method and application. In the method, polyether F127 polymer aqueous solution is used as a reaction medium, and sodium phytate and copper chloride are added in a certain proportion; in an ice-water bath environment, Through the coordination assembly strategy of phosphate and copper ions, copper phytate gradually nucleates and grows, and is uniformly dispersed in the F127 aqueous solution; finally, the pH of the reaction system is adjusted to neutral, and the reaction solution is gradually transformed into a coagulated form by heating in a water bath. gel state, that is, the nanocomposite hydrogel is prepared. The preparation method of the nanocomposite hydrogel dressing proposed in the present invention is green and environment-friendly, the raw materials are safe, and the cost is low. The hydrogel dressing prepared by the method has excellent mechanical properties and biological activity. The excellent temperature sensitivity, injectability and self-healing properties of the nanocomposite hydrogel ensure that it can be applied in complex wound environments.

The invention discloses a preparation method of an acellular cornea. The method comprises the following steps: 1, cleaning and disinfecting a picked animal eyeball; 2, shearing a cornea, and cleaningthe cornea with normal saline; 3, putting the cornea into deionized water, and shaking to break cells in the cornea; 4, placing the cornea in a hypertonic solution for oscillation treatment, and thenplacing the cornea in a hypotonic solution for oscillation treatment; 5, repeatedly treating the cornea for 2-4 times; 6, performing dehydration treatment; and 7, performing cutting, sterilizing and storing. The method disclosed by the invention is adopted to prepare the acellular cornea, has low energy consumption and short period, can thoroughly remove cells in a short time and protect the composition, mechanical integrity and biological activity of collagen of the cornea at the same time, and has no toxic or harmful reagent residues. The cornea can better play the function and transplantation effectiveness, and the prepared acellular cornea is good in transparency, low in immunogenicity and capable of being directly used for corneal transplantation.

A zinc glycyrrhizate film for treating oral ulcer is composed of an adhesive layer prepared from zinc glycyrrhizate and matrix made of carboxymethyl cellulose sodium, polyvinyl alcohol and plasticizer, an a protective layer prepared from polyvinyl alcohol and plasticizer.