33results about How to "Avoid Potential Toxicity" patented technology

The present invention provides methods for the conditional or regulated expression of a site-specific recombinase using split intein-mediated protein splicing. This enhances the temporal and tissue-specificity of trait gene expression and allows for fine tuning of expression specificity.

The present invention relates to stable, preservative- and antioxidant-free liquid formulations of phenylephrine and ketorolac for injection.

The present invention relates to a method of genome engineering in microalgae using the Cas9 / CRISPR system. In particular, the present invention relates to methods of delivering RNA guides via cell penetrating peptides in microalgae, preferably in stable integrated Cas9 microalgae. The present invention also relates to kits and isolated cells comprising Cas9, split Cas9 or guide RNA and Cas9-fused cell-penetrating peptides. The present invention also relates to isolated cells obtained by the methods of the invention.

The present invention relates to stable, preservative- and antioxidant-free liquid formulations of phenylephrine and ketorolac for injection.

The invention relates to a new drug combination for treating diseases of traumatology, a preparation method and application of the drug combination, a medicinal preparation containing the drug combination and quality control method thereof. The drug combination provided by the invention can quickly and durably relieve pains, obviously and rapidly alleviate edema, facilitate callus growth and accelerate fracture healing; compared with the drug combination prepared by the prior art, the new drug combination has better curative effect and high safety.

The invention provides a preparation method of carrier-free macrolide immunosuppressive drug nanoparticles. The carrier-free macrolide immunosuppressive drug nanoparticles are self-assembled to form micromolecular carrier-free nanoparticles through a solvent exchange method, so that the water solubility of a macrolide immunosuppressive drug is effectively improved, and the concentration dependencyinhibits the growth of tumor cells. The method has the advantages that the operation is convenient, simple and practical, and the prepared nanoparticles are small in particle size, high in drug-loading efficiency and good in stability, thereby improving the availability of the macrolide immunosuppressive drug.

The invention discloses a method for preparing a DNA nanopreparation used for treating tumors via a combination of immunotherapy and chemotherapy. The method mainly comprises the following steps: (1)preparing a DNA-A@nucleic acid aptamer polymer chain; (2) preparing a CPG-ODN polymer chain; (3) preparation of a DNA microgel nanoparticle through cross-linking of the DNA-A polymer chain, the CPG-ODN polymer chain and Linker DNA since the DNA-A polymer chain and the CPG-ODN polymer chain are in base complementary with the Linker DNA base, wherein the DNA-A and CPG-ODN are linked with the LinkerDNA by utilizing a DNA base complementary pairing principle so as to form the microgel particles through crosslinking; and 4) loading of a chemotherapeutic drug, i.e., DOX. A DNA-A solution is mixed with an aptamer to form the DNA-A polymer chain with the aptamer. The microgel nanoparticle combines chemotherapy and immunotherapy together, so tumor treatment effect is greatly enhanced, and tumor treatment is more thorough.

The invention provides a polypeptide for preparing hydrogel. The amino acid sequence of the polypeptide is SEQ ID NO: 1. The polypeptide is used for preparing the hydrogel. The prepared hydrogel can be used for producing cell culture holders. The polypeptide has the advantages that enzymes of organisms are catalyzed under physiological conditions to form the hydrogel, and accordingly, damage of external chemical agents, ultraviolet light and the like to tissues is avoided; the biological enzymes can be degraded, the hydrogel is degraded by endogenous substances in the organisms, and accordingly, potential toxicity and immunogenicity risks caused by introduction of exogenous chemical agents are avoided; the polypeptide is an ideal tissue engineering material and is significant to human life health.

A preparation method of straight chain dextrin embedded conjugated linoleic acid microcapsules comprises the following steps: dissolving waxy starch in water, heating for gelatinizing, cooling, adding isoamylase for complete debranching, centrifuging, and freeze-drying to obtain straight chain dextrin powder; and dissolving the straight chain dextrin powder, placing the obtained straight chain dextrin solution at a crystallization temperature, dissolving conjugated linoleic acid (CLA) in a preheated anhydrous ethanol solution, adding the obtained CLA solution into the solution, carrying out heat insulation stirring for a certain time, taking out, cooling to room temperature, centrifuging, washing the obtained precipitate by using an anhydrous ethanol / water mixed solution to remove uncompounded CLA, centrifuging, and carrying out vacuum drying on the finally obtained precipitate to obtain a microcapsule embedding material containing the CLA. The method improves the solubility of the straight chain dextrin and CLA compound, the oxidation stability of the CLA and the bioavailability in an aqueous solution, so the problems of low dissolvability of the straight chain starch compound, and easy automatic oxidation and low bioavailability of the CLA are solved, and the microcapsules can be added to functional nutrition reinforcement foods and staple foods as a nutrition reinforcement agent.

A process for the manufacture of butanol, acetone and / or other renewable chemicals is provided wherein the process utilises one or more of the group comprising by-products of the manufacture of malt whisky, such as pot ale and / or spent lees, biomass substrates, such as paper, sludge from paper manufacture and spent grains from distillers and brewers, and diluents, such as water and spent liquid from other fermentations. The process comprises treating a substrate to hydrolyse it and fermenting the treated. Also provided is a biofuel comprising butanol manufactured according to the process of the invention.

The present invention provides a polypeptide for preparing a hydrogel, the amino acid sequence of which is SEQ ID NO: 1; the polypeptide of the present invention is used for preparing a hydrogel; the prepared hydrogel can be used for preparing a cell culture scaffold. Under physiological conditions, the polypeptide of the present invention is catalyzed by the enzyme of the organism itself to form a hydrogel, which avoids the damage to the tissue caused by external chemical agents, ultraviolet light and the like. And it can be degraded by biological enzymes, realizing the degradation of the gel by endogenous substances in the organism, avoiding the potential toxicity and immunogenicity risks caused by the introduction of exogenous chemical agents; it is an ideal tissue engineering material. Human life and health are of great significance.

The invention relates to a waterborne polyketone resin emulsion, and a preparation method and application thereof, belonging to the technical field of polyketone resin. The waterborne polyketone resinemulsion is prepared from polyketone resin, an isocyanate monomer, a catalyst, a monoamine polymerization inhibitor, an aqueous sulfamate solution, a ketone solvent and water through polymerization,chain extension, emulsification and pressure reduction for solvent removal. The waterborne polyketone resin emulsion prepared in the invention can be used for preparing aqueous ink and coatings, doesnot contain any organic solvent, is environmentally friendly and human-friendly, and reduces the safety hazard of proneness to combustion and explosion during transportation and usage; and the prepared waterborne polyketone resin emulsion avoids potential toxicity of organic solvent residues to packaged food, improves an printing operation environment and realizes the clean packaging when appliedto preparation of waterborne food packaging ink.

The invention provides a preparation method and application of carrier-free double-drug self-assembled nanoparticles. A chemotherapeutic drug sorafenib and a natural product ursolic acid molecule are self-assembled by a solvent exchange method through a pi-alkyl bond, an alkyl bond and a hydrogen bond to form nanoparticles, then indocyanine green is physically adsorbed into the nanoparticles, and the surfaces of the nanoparticles are modified with nucleic acid aptamers and cell-penetrating peptides, so that the indocyanine green nanoparticles are prepared. Thus, the carrier-free double-drug self-assembled nanoparticles are prepared. Potential toxicity caused by introduction and co-modification of a traditional polymer or inorganic carrier is effectively avoided, and a new way is provided for application of a nanotechnology to the field of cancer treatment.

The invention discloses a method for preparing a DNA nano preparation for photo-thermal-immune combined treatment of tumors. The method mainly comprises the following steps: (1) a DNA-A and nucleic acid aptamer polymer chain is prepared; (2) a CPG-ODN polymer chain is prepared; (3) the DNA-A polymer chain and the CPG-ODN polymer chain are complementary with a Linker DNA base, and the chains and the Linker DNA base can be crosslinked to form DNA microgel nanoparticles, and meanwhile, an indocyanine green (ICG) photo-thermal agent is added, and ICG-coated microgel particles are formed through crosslinking; and (4) loading of a chemotherapeutic drug DOX is carried out. A nucleic acid aptamer is mixed in a DNA-A solution to form a DNA-A polymer chain with a nucleic acid aptamer. The microgel nanoparticles combine chemotherapy and immunotherapy, so that the tumor treatment effect is greatly enhanced, and tumor treatment is more thorough.

The present disclosure relates to an antimicrobial peptide conjugated with nanoparticles, in particular the antimicrobial peptide LL37-conjugated gold nanoparticles for use in medicine or cosmetic, for human or animals, namely in the treatment of wound healing or in the treatment of ischemic or vascular diseases, comprising a gold nanoparticle and a plurality of LL-37 peptides, wherein the plurality of LL-37 peptides is bound to the gold nanoparticle surface.

The invention discloses an enzyme-modified anticoagulant valve and a preparation method thereof. The preparation method is as follows: (1) put the biological valve material in the mixed solution of polyphenol compound and soluble metal salt, react at 20-40°C for 5-60 minutes, take it out and wash it; (2) use the product obtained in step (1) as Substrate, after repeating step (1) 1 to 20 times, take it out, wash it and dry it at room temperature; (3) place the product obtained in step (2) in a glutaraldehyde solution with a concentration of 0.1 to 2.5% and activate it for 2 to 10 hours , placed in an enzyme solution with a concentration of 0.01-10 mg / mL that can use glucose to produce hydrogen peroxide, activated at 1-15°C for 4-12 hours, then taken out and washed. The present invention utilizes the characteristics of the valve itself to modify the polyphenol-metal chelate and glucose oxidase to easily and efficiently meet the requirement of long-acting anticoagulation in the valve body and reduce the failure rate of the valve.

The invention relates to a novel medicine composition for treating traumatology diseases, a preparation method of the medicine composition, application, a medicine preparation comprising the medicine composition and a quality control method of the medicine preparation. The medicine composition has functions of rapidly and lastingly relieving pain, obviously and fast subsiding swelling, promoting growth of callus and accelerating fracture healing, and has a better curative effect and higher safety as compared with a medicine composition in the prior art.

The invention discloses a method and application for increasing the ROS level by promoting the conversion of the energy absorbed by the photosensitizer into the CET pathway. The method comprises the following steps: uniformly ultrasonically mix the dissolved photosensitizer and small molecular fluorocarbon compound, then dropwise add it to water, and stir at 40-60°C for 5-10 minutes; the small molecular fluorocarbon compound can drive the photosensitizer to automatically Assembled to form nanoparticles, this self-assembly strategy can reduce the aggregation of photosensitizer molecules, thereby improving the conversion of the energy absorbed by the photosensitizer to the CET pathway, increasing the level of ROS produced by it, thereby enhancing the efficacy of PDT. The invention can effectively solve the problem of insufficient enhancement of CET by encapsulating the photosensitizer by nano-encapsulation technology at present, and increase the content of ROS generated by the photosensitizer.

The invention relates to a novel medicine composition for treating traumatology diseases, a preparation method of the medicine composition, application, a medicine preparation comprising the medicine composition and a quality control method of the medicine preparation. The medicine composition has functions of rapidly and lastingly relieving pain, obviously and fast subsiding swelling, promoting growth of callus and accelerating fracture healing, and has a better curative effect and higher safety as compared with a medicine composition in the prior art.

The invention provides a preparation method of fucoidin self-assembled drug-loaded nanoparticles and application of the fucoidin self-assembled drug-loaded nanoparticles in prevention of renal injury. The preparation method comprises the following steps: connecting an indissolvable hydrophobic drug to fucoidin through a chemical bond, and then entrapping the hydrophobic drug through self-assembly to obtain the self-assembled nanoparticles, and specifically comprises the following steps: adding fucoidin powder into a proper amount of deionized water, carrying out ultrasonic-assisted dissolution and stirring to obtain a fucoidin solution with the concentration of 10 mg / ml; the preparation method comprises the following steps: dissolving a hydrophobic drug in a trace amount of ethanol (the volume ratio of ethanol to water is 1: 10-1: 20), dropwise adding the hydrophobic drug into a fucoidin solution by using an anti-solvent method to prepare self-assembled nanoparticles, and stirring and volatilizing ethanol by using a solvent volatilization method to obtain the nanoparticles with the pH value of 6-7, the particle size of about 150nm and the maximum drug loading capacity of 60%. According to the invention, fucoidin with good biocompatibility is used as a transport carrier, and the self-assembled nanoparticles containing hydrophobic drugs such as ferulic acid, caffeic acid and high polymer procyanidine are obtained. The carrier material selected by the method has functionality, so that the drug stability can be improved, and the dosage and toxicity can be reduced. The method is simple to operate, simple in equipment and suitable for industrial production, and has good social value and application prospect.

The invention provides a preparation method of a carrier-free macrolide immunosuppressive drug nanoparticle. The carrier-free macrolide immunosuppressive drug nanoparticles are self-assembled to form small-molecule carrier-free nanoparticles through a solvent exchange method, which effectively improves the water solubility of the macrolide immunosuppressive drug and inhibits concentration-dependently. growth of tumor cells. The method is convenient to operate, simple and easy to operate, the prepared nanoparticles have small particle size, high drug loading efficiency and good stability, thereby improving the availability of macrolide immunosuppressive drugs.

The invention discloses a nanocomposite hydrogel dressing and its preparation method and application. In the method, polyether F127 polymer aqueous solution is used as a reaction medium, and sodium phytate and copper chloride are added in a certain proportion; in an ice-water bath environment, Through the coordination assembly strategy of phosphate and copper ions, copper phytate gradually nucleates and grows, and is uniformly dispersed in the F127 aqueous solution; finally, the pH of the reaction system is adjusted to neutral, and the reaction solution is gradually transformed into a coagulated form by heating in a water bath. gel state, that is, the nanocomposite hydrogel is prepared. The preparation method of the nanocomposite hydrogel dressing proposed in the present invention is green and environment-friendly, the raw materials are safe, and the cost is low. The hydrogel dressing prepared by the method has excellent mechanical properties and biological activity. The excellent temperature sensitivity, injectability and self-healing properties of the nanocomposite hydrogel ensure that it can be applied in complex wound environments.

The invention discloses a preparation method of an acellular cornea. The method comprises the following steps: 1, cleaning and disinfecting a picked animal eyeball; 2, shearing a cornea, and cleaningthe cornea with normal saline; 3, putting the cornea into deionized water, and shaking to break cells in the cornea; 4, placing the cornea in a hypertonic solution for oscillation treatment, and thenplacing the cornea in a hypotonic solution for oscillation treatment; 5, repeatedly treating the cornea for 2-4 times; 6, performing dehydration treatment; and 7, performing cutting, sterilizing and storing. The method disclosed by the invention is adopted to prepare the acellular cornea, has low energy consumption and short period, can thoroughly remove cells in a short time and protect the composition, mechanical integrity and biological activity of collagen of the cornea at the same time, and has no toxic or harmful reagent residues. The cornea can better play the function and transplantation effectiveness, and the prepared acellular cornea is good in transparency, low in immunogenicity and capable of being directly used for corneal transplantation.

A zinc glycyrrhizate film for treating oral ulcer is composed of an adhesive layer prepared from zinc glycyrrhizate and matrix made of carboxymethyl cellulose sodium, polyvinyl alcohol and plasticizer, an a protective layer prepared from polyvinyl alcohol and plasticizer.

The invention discloses a fully automatic cell non-stained image recognition and counting method, which selects the image recognition method automatic cell counter, directly adds the cell suspension to be tested into the counting sheet and inserts it into the counter, and reduces the red light R of the photosensitive chip. , the receiving ability of green light G light intensity, the operation of enhancing the receiving ability of blue light B light intensity, and manual adjustment of exposure time and exposure gain, so that the background of the display is light blue, and the circular bright spot is the image of living cells, while The circular blue spot darker than the background blue is the image of dead cells. Through the analysis of cell image recognition software, accurate analysis data of cell activity of various sizes can be obtained. The invention can count the activity of the cells without the use of dyes, avoiding the potential toxicity of the cells to be tested by using trypan blue staining, the cells after detection can continue to survive, and the activity can be counted during the culture process. At the same time, the invention can realize non-toxic Stained real-time online cell counting can be widely used in the field of life science research.

The invention relates to a method for preparing three-dimensional randomly oriented fibers. The preparation method includes: preparing a spinning solution; wherein the components of the spinning solution include polymers and high-conductivity materials; performing electrostatic spinning on the above spinning solution, and post-processing to obtain . The invention develops a three-dimensional electrospinning technology based on a high-conductivity spinning system, which has high efficiency, good material universality, low requirements on the spinning environment and continuous production, which can promote the more fine fibers in various fields. for a wide range of application requirements.

The invention discloses a method for improving the ROS level by promoting transformation of energy absorbed by a photosensitizer toward a CET pathway and application. The method comprises the following steps: uniformly mixing a dissolved photosensitizer and a micromolecular fluorocarbon by ultrasonics, dropwise adding the mixture into water, and stirring the mixture at 40-60 DEG C for 5-10 minutes. The micromolecular fluorocarbon can drive self-assembly of the photosensitizer to form nanoparticles, and the self-assembly strategy can reduce aggregation among photosensitizer molecules, so that transformation of energy absorbed by the photosensitizer toward the CET pathway is improved, the level of ROS generated by the photosensitizer is promoted, and the PDT curative effect is enhanced. The method can effectively solve the problem of insufficient CET enhancement of the photosensitizer wrapped by a nano packaging technology at present, and can promote the content of ROS generated by the photosensitizer.

The present invention provides a method for nitriding a MOSFET device, comprising nitriding the MOSFET device with nitrogen-containing gas, preferably the nitriding treatment is performed at a temperature of 1200-1500°C, preferably 1250-1450°C. According to the nitriding method of the power device provided by the present invention, nitrogen is used for nitriding at a high temperature during the oxidation process and / or after oxidation of the device, which simplifies the nitriding passivation gas system, avoids gate dielectric reliability and breakdown electric field strength The reduction of potential toxicity and poisonous gas leakage risk caused by nitriding gas is avoided, and the exhaust gas treatment system is simplified.