8results about How to "Avoid Potential Toxicity" patented technology

The invention relates to a universal hydrogel vaccine based on tumor neoantigen peptide as well as a preparation method and application of the universal hydrogel vaccine. The hydrogel vaccine comprises tumor neoantigen peptide hydrogel and an adjuvant loaded on the tumor neoantigen peptide hydrogel, the tumor neoantigen peptide-peptidyl assembly sequence conjugate is assembled in a solvent to form a nanofiber solution, and the nanofiber solution is triggered to form hydrogel under physiological conditions; the conjugate is obtained by coupling a tumor neoantigen peptide and a peptidyl assembly sequence through a covalent bond; the adjuvant is at least any one of a cell factor, an STING agonist, a Toll-like receptor agonist and an immune cell. The vaccine disclosed by the invention has universality, aiming at different tumor neoantigen peptides, self-assembly of a conjugate in a solvent is realized by regulating and controlling the quantity of valine or phenylalanine in a peptidyl assembly sequence to form a nanofiber solution, and the vaccine adjuvant is loaded through physical action to obtain a nanofiber solution containing the vaccine adjuvant; therefore, personalized cancer immunotherapy is realized.

The present application discloses a regeneration-free high-efficiency carbon-containing aluminum defluoridation agent and a preparation method thereof. The dried sodium metaaluminate powder and nano-oxide are placed in a beaker, an organic acid solution is added, and the solution after stirring is poured into a hydrothermal reactor, subjected to hydrothermal reaction at 140-240° C., the reactor is naturally cooled, a sample in the reactor is taken out and centrifuged, washed once with distilled water, centrifuged again and washed once with ethanol, and centrifuged again and washed once with distilled water; the sample is placed in a vacuum drying oven and vacuum dried at 70-80° C. The sample is placed in a muffle furnace and pyrolyzed to obtain a regeneration-free high-efficiency carbon-containing aluminum defluoridation agent, which does not require chemical agent regeneration and can be repeatedly recycled; in practical applications, the operation complexity and use cost are reduced, while the environmental pollution problem that may be caused by the regeneration process is avoided, and the preparation of an efficient, rapid, low-cost, and non-toxic defluoridation adsorbent is realized, the defluoridation efficiency is improved, and a sustainable solution is provided for solving the problem of excessive fluoride in drinking water.

The invention belongs to the field of prodrug compounds, and particularly relates to a prodrug compound of a cuprous-responsive copper dithiocarbamate complex as well as a preparation method and application of the prodrug compound. According to the compound disclosed by the invention, after the organic ligand in the structure is coordinated with the cuprous, an oxidation bond breaking reaction catalyzed by metal ions can be carried out in the presence of oxygen, and the cuprous is converted into cuprous along with the conversion of the cuprous into the cuprous. Then, dithiocarbamic acid formed by breakage can be competitively combined with bivalent copper ions, so that the dithiocarbamic acid copper complex with high biological activity is converted. The design can play a role in related indications such as tumor prevention and treatment, metal ion metabolism abnormality and the like.

The application discloses an implant device interface system capable of realizing rapid cell adhesion and controllable release, a method and application, and relates to the technical field of biomedical materials and tissue engineering. The interface system comprises a first reaction group fixed on the surface of an implant device and a second reaction group inserted into the membrane of target cells through a membrane anchoring molecule; the membrane anchoring molecule comprises a cleavable linker; the first and second reaction groups constitute a complementary system such as click chemistry or biological orthogonality. The system realizes rapid adhesion within 0.5-6 hours, the cell migration rate increases after 6 hours of triggering, and a monolayer is formed within 12-48 hours, the cell tight junction is enhanced, and the blood compatibility is good (hemolysis rate < 5%). The system is applicable to different materials and cell types, and is suitable for surface cell construction and biological integration improvement of small-diameter artificial blood vessels, vascular stents, catheters, valves and other devices.

The invention discloses a GSH (glutathione) responsive capsaicin prodrug which is capsaicin dimer nano-particles formed by performing esterification reaction on capsaicin and dithiodipropionic acid and performing self-assembly, and further discloses a preparation method of the prodrug. The capsaicin dimer nano-particles prepared by the invention can react with glutathione highly expressed at an inflammation part so as to release capsaicin, and the prodrug has a certain targeting property, so that the side effect of the drug can be reduced, and the curative effect can be improved. In addition, the prodrug is not easy to be metabolized and discharged, and is more stable in plasma, so that the half-life period and bioavailability of the drug can be improved, and the administration frequency is reduced. An in-vitro cell test result shows that compared with capsaicin, the content of inflammatory factors in cells can be remarkably reduced, and a better anti-inflammatory effect is achieved.

This invention discloses a calcium phosphate ceramic particle-methacrylated gelatin hydrogel scaffold for osteoblasts pretreated with Tideglusib to enhance osteogenic differentiation, relating to the fields of biomedical engineering and bone tissue regeneration. It comprises osteoblasts pretreated with Tideglusib and activated, biphasic calcium phosphate ceramic particles, and a methacrylated gelatin hydrogel encapsulating the osteoblasts and ceramic particles. The preparation method includes: obtaining rat osteoblasts; seeding the osteoblasts onto the biphasic calcium phosphate ceramic particles; and pretreating the seeded biphasic calcium phosphate ceramic particles with Tideglusib solution to activate the Wnt signaling pathway and promote osteogenic differentiation. This invention exhibits excellent bone defect repair capabilities, good degradability and biocompatibility, provides necessary mechanical support, and has strong clinical translational potential.

This invention relates to an electroactive casein bio-ink, its preparation method, and its applications, belonging to the field of biomedical materials technology. The ink uses quaternized methacrylamide casein (QCMA) as its core component, combined with a photoinitiator and chondrocytes. The preparation method involves first introducing quaternization into the casein to introduce cationic groups, then grafting photosensitive double bonds via methacrylation, purifying the resulting QCMA powder, and finally compounding it with a photoinitiator and cells to form the ink. This bio-ink possesses both high transparency and rapid photocuring properties, making it suitable for high-precision DLP printing. The constructed hydrogel exhibits intrinsic ionic conductivity, enabling the reconstruction of the electrophysiological microenvironment of damaged tissues and acting as an "electric bridge." Simultaneously, its quaternary ammonium groups endow the material with broad-spectrum antibacterial capabilities and promote cell adhesion and matrix deposition. The material of this invention is applicable to tissue regeneration fields such as cartilage defect repair.

This invention discloses a method for preparing and applying right-handed molybdenum-doped carbon dots. The method involves using D-tartaric acid, ethylenediamine, and molybdenum salt as raw materials, followed by hydrothermal reaction, centrifugation, dialysis, and lyophilization to obtain D-Mo-CDs. These carbon quantum dots exhibit selective response to circularly polarized light; D-Mo-CDs are specifically activated under left-handed circularly polarized light irradiation, achieving a photothermal conversion efficiency of 51.2%, and possessing highly efficient reactive oxygen species generation capabilities, enabling photodynamic-photothermal synergistic antibacterial effects. This invention also discloses the application of these carbon quantum dots in the preparation of spatiotemporally precise antibacterial materials and in medical devices or dressings for treating bacterial infected wounds. This material exhibits high biocompatibility, significant in vitro antibacterial effects, and promotes wound healing in vivo, providing a novel integrated solution for the treatment of infected wounds.