Acellular cornea and preparation method thereof

Abstract

The invention discloses a preparation method of an acellular cornea. The method comprises the following steps: 1, cleaning and disinfecting a picked animal eyeball; 2, shearing a cornea, and cleaningthe cornea with normal saline; 3, putting the cornea into deionized water, and shaking to break cells in the cornea; 4, placing the cornea in a hypertonic solution for oscillation treatment, and thenplacing the cornea in a hypotonic solution for oscillation treatment; 5, repeatedly treating the cornea for 2-4 times; 6, performing dehydration treatment; and 7, performing cutting, sterilizing and storing. The method disclosed by the invention is adopted to prepare the acellular cornea, has low energy consumption and short period, can thoroughly remove cells in a short time and protect the composition, mechanical integrity and biological activity of collagen of the cornea at the same time, and has no toxic or harmful reagent residues. The cornea can better play the function and transplantation effectiveness, and the prepared acellular cornea is good in transparency, low in immunogenicity and capable of being directly used for corneal transplantation.

Description

technical field [0001] The invention belongs to the technical field of medical devices, and in particular relates to an acellular cornea and a preparation method thereof. Background technique [0002] The cornea is the most transparent part of the eye, covering the iris, pupil and anterior chamber. Due to the complex and interlaced collagen structure, it can maintain good transparency and diopter, and at the same time, the rich and dense distribution of nerves also enables people to better perception of the world around them. However, corneal lesions will have a certain impact on its transparency. According to statistics, eye diseases caused by corneal diseases rank second in the world, and are still increasing. Faced with a large patient population, especially in Asian countries, the number of donated corneas is relatively small, so the hope of transplantation based on corneal donation alone is very small. [0003] In the search for alternatives to corneal transplantation...

AI Technical Summary

Problems solved by technology

However, whether the decellularization agent destroys the key structure of the extracellular matrix is ​​not clear, and the residue of the decellularization agent also reduces the histocompatibility and greatly increases the risk of allograft transplantation

For example, the acid in the decellularization reagent will damage the collagen and reduce the mechanical properties of the cornea; enzyme decellularization has strong specificity, and it needs to be combined with other methods for decellularization, but the residue of enzymes will not only affect cell regeneration, It can also cause the body's immune response; although the non-ionic detergent TritonX-100 is better than enzymes, it is more toxic and will reduce the mechanical properties of the cornea; the ionic detergent SDS or SD has greater toxicity, and it cannot be used alone. Removing soluble protein antigens in the cornea will also damage the extracellular matrix components; the use of alcohols will precipitate collagen and affect the submicroscopic structure; and chelating agents such as EDTA alone are not effective and will cause the loss of matrix many metal ions

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