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A kind of enzyme-modified anticoagulant valve and its preparation method

An anti-coagulation and modification technology, applied in the field of biomedical engineering functional materials, can solve the problems of difficult long-term storage and high price, achieve the effects of easy preparation, reduce valve failure rate, and avoid unstable donor sources

Active Publication Date: 2021-11-23
JILIN VENUS HAOYUE MEDICAL LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a natural enzyme, nitric oxide synthase is highly specific and efficient, but its high price and difficulty in long-term storage limit its industrial application.

Method used

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  • A kind of enzyme-modified anticoagulant valve and its preparation method
  • A kind of enzyme-modified anticoagulant valve and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A preparation method of an enzyme-modified anticoagulant valve, comprising the following steps:

[0028] (1) Put the valve into a mixed aqueous solution of dopamine with a concentration of 1 mg / ml and ferric chloride with a concentration of 0.5 mg / ml, react at 25°C for 20 minutes, take out the valve and wash it 3 times, and complete a reaction cycle;

[0029] (2) Repeat the reaction cycle 10 times and dry;

[0030] (3) Soak the material treated in step (2) in a glutaraldehyde solution with a concentration of 0.5% and activate it for 4 hours and then wash it;

[0031] (4) Put the material obtained in step (3) into a PBS solution of glucose oxidase at a concentration of 0.15 mg / ml, activate at 4° C. for 4 hours, and wash to obtain the target material.

Embodiment 2

[0033] A preparation method of an enzyme-modified anticoagulant valve, comprising the following steps:

[0034] (1) Put the valve into a mixed aqueous solution of tannic acid with a concentration of 1 mg / ml and ferric chloride with a concentration of 0.1 mg / ml, react at 30°C for 10 minutes, take out the valve and wash it 3 times, and complete a reaction cycle;

[0035] (2) Repeat the reaction cycle 5 times and dry;

[0036] (3) Soak the material treated in step (2) in a glutaraldehyde solution with a concentration of 0.15% and activate it for 2 hours before washing;

[0037] (4) Put the material obtained in step (3) into a PBS solution of glucose oxidase at a concentration of 0.2 mg / ml, activate at 4° C. for 4 hours, and wash to obtain the target material.

Embodiment 3

[0039] A preparation method of an enzyme-modified anticoagulant valve, comprising the following steps:

[0040] (1) Put the valve into a mixed aqueous solution of tannic acid with a concentration of 1 mg / ml and copper chloride with a concentration of 0.1 mg / ml, react at 30°C for 20 minutes, take out the valve and wash it 3 times, and complete a reaction cycle;

[0041] (2) Repeat the reaction cycle 5 times and dry;

[0042] (3) Soak the material treated in step (2) in a glutaraldehyde solution with a concentration of 0.2% and activate it for 2 hours before washing;

[0043] (4) Put the material obtained in step (3) into a PBS solution of glucose oxidase at a concentration of 0.2 mg / ml, activate at 4° C. for 4 hours, and wash to obtain the target material.

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Abstract

The invention discloses an enzyme-modified anticoagulant valve and a preparation method thereof. The preparation method is as follows: (1) put the biological valve material in the mixed solution of polyphenol compound and soluble metal salt, react at 20-40°C for 5-60 minutes, take it out and wash it; (2) use the product obtained in step (1) as Substrate, after repeating step (1) 1 to 20 times, take it out, wash it and dry it at room temperature; (3) place the product obtained in step (2) in a glutaraldehyde solution with a concentration of 0.1 to 2.5% and activate it for 2 to 10 hours , placed in an enzyme solution with a concentration of 0.01-10 mg / mL that can use glucose to produce hydrogen peroxide, activated at 1-15°C for 4-12 hours, then taken out and washed. The present invention utilizes the characteristics of the valve itself to modify the polyphenol-metal chelate and glucose oxidase to easily and efficiently meet the requirement of long-acting anticoagulation in the valve body and reduce the failure rate of the valve.

Description

technical field [0001] The invention belongs to the technical field of biomedical engineering functional materials, and in particular relates to an enzyme-modified anticoagulant valve and a preparation method thereof. Background technique [0002] In view of the clinical situation of valvular insufficiency or failure, it is of great market value to develop artificial valves that can be used to treat valvular regurgitation diseases. Under physiological conditions, the valve is thin and is easily affected by the blood flow pressure around the valve leaflet. Currently, the main cause of valve failure is thrombus. Immediately after the valve is implanted in the body, a series of coagulation reactions will be triggered, such as the deposition of coagulation factors and adhesive proteins, the adsorption and denaturation of fibrinogen, and the aggregation and activation of platelets. [0003] Endothelium-derived relaxation factor Nitric oxide (NO) is an important anticoagulant fac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/04A61L27/36A61L33/02A61L33/04A61L33/16
CPCA61L27/3625A61L27/3687A61L33/0047A61L33/0076A61L33/02A61L33/04A61L2430/20A61L2400/18C08L89/00
Inventor 杨立王云兵罗日方
Owner JILIN VENUS HAOYUE MEDICAL LTD
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