A kind of preparation method of linear dextrin-embedded conjugated linoleic acid microcapsules

A technology of conjugated linoleic acid and linear dextrin, which is applied in the direction of microcapsule preparation and microsphere preparation, can solve the problems of poor solubility of amylose complexes, CLA easy auto-oxidation bioavailability, etc., and achieve low cost , small molecular weight, good solubility

Active Publication Date: 2016-03-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method provides a new type of embedding wall material, which improves the solubility of the linear dextrin and CLA complex, the oxidation stability of CLA and the bioavailability in aqueous solution, thereby solving the problem of the poor solubility of the amylose complex , CLA is easy to automatically oxidize and have low bioavailability. It can be added as a nutritional fortifier to functional nutritionally fortified foods and staple foods.

Method used

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  • A kind of preparation method of linear dextrin-embedded conjugated linoleic acid microcapsules
  • A kind of preparation method of linear dextrin-embedded conjugated linoleic acid microcapsules
  • A kind of preparation method of linear dextrin-embedded conjugated linoleic acid microcapsules

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Embodiment 1

[0039] The application provides a method for preparing microcapsules of linear dextrin-embedded conjugated linoleic acid, the specific steps are as follows:

[0040] (1) Evenly disperse 1g of waxy starch in 90mL of distilled water, stir and pregelatinize in a boiling water bath, then cook at 121°C under high pressure for 20min until completely gelatinized, then cool to room temperature;

[0041](2) Add 10mL sodium acetate buffer (0.1M, PH=3.5) and 7.5U / g starch isoamylase to the gelatinized waxy cornstarch for debranching, the reaction temperature is 40℃, and the reaction time is After 12 hours of complete debranching, boil the hydrolyzed solution in a boiling water bath for 10 minutes to inactivate the enzyme, centrifuge at 5000 r / min for 5 minutes to remove the enzyme, and freeze-dry to powder after rotary evaporation to obtain linear dextrin.

[0042] (3) Add 500mg of linear dextrin powder to 5mL of 1M NaOH solution to dissolve, then add 40ml of distilled water to dilute, a...

Embodiment 2

[0051] The application provides a method for preparing microcapsules of linear dextrin-embedded conjugated linoleic acid, the specific steps are as follows:

[0052] (1) Evenly disperse 1g of waxy starch in 90ml of distilled water, stir and pregelatinize in a boiling water bath, then cook at 110°C for 30min under high pressure until it is completely gelatinized, then cool to room temperature;

[0053] (2) Add 10mL sodium acetate buffer (0.1M, PH=3.5) and 1U / g starch isoamylase to the gelatinized waxy cornstarch for debranching, the reaction temperature is 30℃, and the reaction time is 48h After complete debranching, boil the hydrolyzed solution in a boiling water bath for 10 minutes to inactivate the enzyme, centrifuge at 5000 r / min for 5 minutes to remove the enzyme, and freeze-dry to powder after rotary evaporation to obtain linear dextrin.

[0054] (3) Add 500mg of straight-chain dextrin powder to 10mL of 1M NaOH solution to dissolve, then add 80ml of distilled water to dil...

Embodiment 3

[0058] The application provides a method for preparing microcapsules of linear dextrin-embedded conjugated linoleic acid, the specific steps are as follows:

[0059] (1) Evenly disperse 1g of waxy starch in 90ml of distilled water, stir and pregelatinize in a boiling water bath, then cook at 130°C for 10min under high pressure until completely gelatinized, then cool to room temperature;

[0060] (2) Add 10mL sodium acetate buffer (0.1M, PH=3.5) and 10U / g starch isoamylase to the gelatinized cornstarch for debranching, the reaction temperature is 60℃, and the reaction time is 12h; complete After debranching, boil the hydrolyzed solution in a boiling water bath for 10 minutes to inactivate the enzyme, centrifuge at 5000 r / min for 5 minutes to remove the enzyme, and freeze-dry to powder after rotary evaporation to obtain linear dextrin.

[0061] (3) Add 500mg of linear dextrin powder to 1.7mL of 1M NaOH solution to dissolve, then add 13.3ml of distilled water to dilute, and final...

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Abstract

A preparation method of straight chain dextrin embedded conjugated linoleic acid microcapsules comprises the following steps: dissolving waxy starch in water, heating for gelatinizing, cooling, adding isoamylase for complete debranching, centrifuging, and freeze-drying to obtain straight chain dextrin powder; and dissolving the straight chain dextrin powder, placing the obtained straight chain dextrin solution at a crystallization temperature, dissolving conjugated linoleic acid (CLA) in a preheated anhydrous ethanol solution, adding the obtained CLA solution into the solution, carrying out heat insulation stirring for a certain time, taking out, cooling to room temperature, centrifuging, washing the obtained precipitate by using an anhydrous ethanol / water mixed solution to remove uncompounded CLA, centrifuging, and carrying out vacuum drying on the finally obtained precipitate to obtain a microcapsule embedding material containing the CLA. The method improves the solubility of the straight chain dextrin and CLA compound, the oxidation stability of the CLA and the bioavailability in an aqueous solution, so the problems of low dissolvability of the straight chain starch compound, and easy automatic oxidation and low bioavailability of the CLA are solved, and the microcapsules can be added to functional nutrition reinforcement foods and staple foods as a nutrition reinforcement agent.

Description

technical field [0001] The invention relates to the technical field of functional food additives, in particular to a method for embedding conjugated linoleic acid with straight-chain dextrin. Background technique [0002] Conjugated linoleic acid (CLA) is a free fatty acid with conjugated double bonds. It is a natural active nutrient commonly found in humans and animals. It has anti-cancer, anti-atherosclerosis, anti-oxidation, improving immunity, It has many physiological functions such as promoting growth and reducing body fat, and has broad application prospects in the fields of medicine, food, and health care products. However, conjugated linoleic acid has poor water solubility and limited utilization in aqueous systems; it has poor chemical stability and is easily oxidized and deteriorated by air, and the formed oxidation products lead to product quality deterioration and shortened shelf life; therefore, it is not conducive to production. storage, transport and applica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/02
Inventor 周星金征宇常情情孟令儒顾丽敏田耀旗焦爱权徐学明
Owner JIANGNAN UNIV
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