This invention aims to provide an
antifreeze starch microsphere, its preparation method, and its application in frozen glutinous rice balls. Specifically, using
waxy starch as
raw material, the process involves high-temperature pregelatinization,
pullulan enzymatic debranching, and stepwise
alcohol precipitation and
fractionation to introduce phenolic acids to form a single-
helix complex with
starch molecules. Subsequently, entropy reduction is used to promote the orderly arrangement of molecular chains, ultimately yielding spherical
starch microspheres with an A-type crystalline structure. The obtained
starch microspheres are mixed with glutinous
rice flour and water in a certain proportion to prepare glutinous rice balls with a
diameter of approximately 2.0 cm. These glutinous rice balls not only have excellent taste and significantly improved digestibility, but also exhibit a significantly reduced
cracking rate after freeze-thaw cycles, thus effectively improving the storage stability and edible quality of frozen glutinous rice balls.