New method for formulation of full waxy starch wheat germplasm

A technology for waxy wheat and wheat, applied in the field of cultivating whole waxy wheat and obtaining new germplasm of whole waxy wheat, which can solve problems such as difficult commercial application and poor agronomic traits
CN102747100AInactive Publication Date: 2012-10-24INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
Publication Date
2012-10-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for cultivation of full waxy wheat. The method for cultivation of full waxy wheat provided in the invention includes the following step of: inhibiting the expression of GBSS (granule-bound starch synthase) I gene in target wheat to obtain the full waxy wheat. The inhibition of expression of the GBSS I gene in the target wheat is realized by transferring a DNA fragment as shown in formula "SEQforward-X-SEQbackward" into the target wheat. Specifically, the SEQforward is the top 288 nucleotides in sequence 1 of a sequence table; the SEQbackward is in reverse complement with the SEQforward; the X is a spacer sequence between the SEQforward and the SEQbackward, and in sequences, the X is not complementary with and the SEQforward and the SEQbackward. Experiments prove that the wheat GBSS I gene can be silenced through a transgene silencing technology (RNAi) to obtain a GBSS I gene expression-inhibiting full waxy wheat new germplasm, and the transgenic material has excellent comprehensive agronomic characters, thus being able to be directly used for selective breeding of wheat low-amylose new strains (varieties) or be used as a patent material of breeding.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a method for cultivating full-waxy wheat, in particular to a method for silencing the wheat granule-bound starch synthase gene (GBSSⅠ) through the transgene silencing technique (RNAi), thereby obtaining a new variety of full-waxy wheat qualitative method. Background technique

[0002] The genetic improvement of wheat starch quality is called "biotechnology to turn starch into gold" abroad, and waxy wheat is one of the research hotspots.

[0003] The grain starch of whole waxy wheat does not contain amylose or contains very little (usually less than 1%). The starch viscosity characteristics of its flour are very different from those of ordinary wheat. It is a new wheat material and can be widely used in food It has great development and application value in industries such as processing, specialty food development, processing raw materials related to starch, papermaking, textiles, and ...

Claims

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