Method for exsomatizing screening grassiness salt resistsomatic mutation body

A technology of somatic cell mutation and screening method, which is applied in the field of in vitro screening of elephant grass salt-tolerant somatic mutants, can solve the problems of different seed maturity stages, easy seed shattering and germination rate, etc., and achieves short cycle, easy operation, and selection high efficiency effect

Active Publication Date: 2010-11-10
JIANGSU ACAD OF AGRI SCI
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Problems solved by technology

Although elephant grass is a perennial herb, it cannot bloom and bear fruit naturally in the middle and lower reaches of the Yangtze River in my country and the regions at the same latitude. asexual reproduction

Method used

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  • Method for exsomatizing screening grassiness salt resistsomatic mutation body
  • Method for exsomatizing screening grassiness salt resistsomatic mutation body

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Embodiment Construction

1.3 NaCl stress screening

The granular callus cultured in the callus induction medium for 28 days was transferred to the callus subculture medium supplemented with 0, 10, 12, 14, 16, 18 and 20 g / L NaCl, and transferred to the supplemented NaCl after 45 days The same concentration of callus differentiation medium. After 35 days of culture, the seedlings with more than 3 leaves were transferred to the rooting medium without NaCl, and the green shoots were transferred to the callus differentiation medium containing the same concentration of NaCl, and the seedling rate was counted at 28 days. The seedlings were moved into a simple greenhouse after 28 days of growth, and the live tree rate was counted after 21 days of growth.

The temperature of the culture room is 26°C-28°C, the light intensity is 2000 lux, and the light is 16 hours a day.

1.4 Preliminary identification of salt tolerance of regenerated plants by screening tissue culture for salt stress

1.4.1 Preliminary ident...

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Abstract

The invention relates to an screening method in vitro of elephant grass salt-tolerant somatic mutant belongs to a field of agricultural biotechnology. The process is: granular callus is transferred into proliferation medium with 16g / L NaCl, into differentiation medium with 16g / L NaCl after 45 days, and into rooting medium with no NaCl after 35 days to get regeneration plant; pot salt-tolerance identification of the regeneration plant is applied after tidal flat field comprehensive agronomic characters evaluation and grass-production measurement to get plants of which the fresh grass yield is higher than a control by 23.8% in tidal flat field with pH8.67-9.01; the fresh grass increment of the first time and the second tiome of cutting for the clonal progeny of the plants is higher than thecontrol by 9.2% and 7.4% respectively in 1 / 2Hoagland nutrient solution with 8.25g / L sea-salt; therefore, the salt-tolerant somatic mutant of good comprehensive agronomic characters and significantly high salt-tolerance is selected. The invention has the advantages of simple-operation, short-cycle, and high selection efficiency.

Description

In Vitro Screening Method of Elephant Grass Salt-tolerant Somatic Mutants 1. Technical field The invention relates to an in vitro screening method for salt-tolerant somatic cell mutants of Elephant grass, which belongs to the field of biotechnology and is specially used for engineering breeding of Elephant grass salt-tolerant cells. 2. Technical background Elephant grass (Pennisetum purpureum Schumach), also known as purple pennisetum, is native to tropical Africa and was introduced into my country in the 1920s. It is especially suitable for perennial grasses planted in tropical and subtropical regions. Studies at home and abroad have shown that elephant grass is not only a high-quality green feed for grass-fed livestock and poultry, an ideal soil and water conservation crop for road slope protection, a high-quality raw material for medium and high-grade pulp and wood-based panels, but also an ideal biomass energy crop. Although elephant grass is a perennial herb, it canno...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/04A01H4/00A01H1/06C12N5/04
Inventor 钟小仙佘建明顾洪如倪万潮张建丽丁成龙
Owner JIANGSU ACAD OF AGRI SCI
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