Method for screening dandelion salt-tolerance mutants

A technology for mutants and dandelion, which is applied in the field of screening dandelion salt-tolerant mutants, can solve the problems of heavy and unreported screening of dandelion salt-tolerant mutants, and achieves the effects of stable mutants, good effects and broad application prospects.

Active Publication Date: 2015-06-24
COASTAL AGRI RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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Problems solved by technology

[0004] To sum up, the screening of salt-tolerant mutants of dandelion is mainly obtained by somatic cell cloning, and the identification of salt-tolerant mutants is mainly carried out by NaCl aqueous solution; Although it has been applied to salt-tolerant breeding of various plants, it has not been reported in the research of screening salt-tolerant mutants of dandelion

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  • Method for screening dandelion salt-tolerance mutants
  • Method for screening dandelion salt-tolerance mutants

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Embodiment 1

[0030] Example 1 Screening of dandelion salt-tolerant mutants

[0031] The medium configuration of this embodiment:

[0032] Induction medium: containing 0.5mg / L 6-benzylaminopurine (6-BA), 0.2mg / L dichlorobenzyloxyacetic acid (2,4-D), 7g / L agar powder and 30g / L sucrose, pH value 5.7-6.0 MS medium.

[0033] Liquid differentiation medium: MS medium containing 1mg / L kinetin (KT), 0.3mg / L naphthaleneacetic acid (NAA), 30g / L sucrose, pH 5.7-6.0;

[0034] Solid differentiation medium: MS medium containing 1mg / L kinetin (KT), 0.3mg / L naphthaleneacetic acid (NAA), 7g / L agar powder, 30g / L sucrose, pH 5.7-6.0.

[0035] Rooting medium: MS medium containing 0.5mg / L naphthaleneacetic acid (NAA), 7g / L agar powder, 30g / L sucrose, pH 5.7-6.0.

[0036] Specific test method:

[0037] 1. Aseptic vaccine culture

[0038] Select plump seeds and treat them with 75% ethanol for 30 seconds, then wash them with sterile water for 3 times, soak them in 0.1% mercury chloride for 12 minutes, then wa...

Embodiment 2

[0053] Identification of embodiment 2 salt-tolerant plants

[0054] Salt tolerance identification of salt-tolerant plants was carried out by potting method with soil full salt content of 0.2%, 0.4%, 0.6%, 0.8%, 1.0% saline-alkali soil, and regenerated plants without mutagenesis and NaCl screening were used as controls , record the leaf length and leaf width of the plant before transplanting, record the survival rate, leaf length and leaf width of the plant again 30 days after transplanting, and observe the growth of the plant.

[0055] The results showed that in the treatment with 0.2% total soil salt content, there was basically no difference between the salt-tolerant plants and the control in terms of survival rate and growth; in the treatment with 0.4% total soil content, the survival rate of the control plants was about 80%, and the salt-tolerant plants were basically All survived, and there was no obvious difference between the two in terms of growth; in the treatment of ...

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Abstract

The invention discloses a method for screening dandelion salt-tolerance mutants. The method comprises the steps that full seeds are selected and cultured; aseptic seedlings are acquired and cut into slices; the slices are subjected to callus induction; embryonic calluses are obtained, sequentially inoculated to a liquid differential medium containing EMS (Ethylmethane Sulfonate) and a solid differential medium containing NaCl, and screened; salt-tolerance test-tube seedlings are obtained; acclimatization and transplant are performed to make the salt-tolerance test-tube seedlings survive; then the salt-tolerance test-tube seedlings are transplanted a greenhouse; and salt-tolerance dandelion plants are obtained with the combination of coastal original saline alkali soil potting saline-alkaline tolerance identification method. The method for screening the dandelion salt-tolerance mutants is simple to operate, good in effect, high in efficiency, and very wide in application prospect, and accelerates a dandelion salt-tolerance breeding course.

Description

technical field [0001] The invention relates to the technical field of salt-tolerant plant breeding, in particular to a method for screening salt-tolerant mutants of dandelion. Background technique [0002] Soil salinization is a major stress factor affecting agricultural production. There are nearly 100 million hectares of saline soil in my country, and the salinization and secondary salinization continue to expand. In order to ensure the sustainable development of human beings, it is necessary to explore effective governance and utilization methods. Traditional governance methods consume a lot of money and water resources with little effect. Years of practice have proved that planting salt-tolerant plants is one of the biological measures to improve saline-alkali land, and it is the most economical, effective and sustainable method in the improvement and utilization of saline-alkali land, which has attracted more and more attention. However, the shortage of salt-tolerant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘雅辉王秀萍张国新鲁雪林李强赵敏刘艳芬梁伟玲
Owner COASTAL AGRI RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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