15results about How to "Improve fermentation performance" patented technology

The invention provides a temperature-resistant and salt-resistant cross-linking tackifying filtrate reducer for a soil-phase-free water-based drilling fluid as well as a preparation method and application of the temperature-resistant and salt-resistant cross-linking tackifying filtrate reducer, and belongs to the technical field of drilling. The temperature-resistant and salt-resistant cross-linking tackifying filtrate reducer for the soilless phase water-based drilling fluid comprises the following raw materials in parts by weight: N, N-dimethylformamide, a cross-linking agent and a cross-linking agent, the invention relates to a water-based adhesive which is prepared from the following components in parts by weight: 15 to 25 parts of N, N-dimethylacrylamide, 1 to 10 parts of 3-[(3-methacrylamidopropyl) dimethylammonium] propane-1-sulfonate, 1 to 10 parts of long-chain alkyl polyoxyethylene ether methacrylate, 0.005 to 0.015 part of macromolecular cross-linking agent, 0.03 to 0.08 part of initiator A and 80 to 100 parts of deionized water. The tackifying filtrate reducer can resist the temperature of 180 DEG C, can resist NaCl to be saturated, has excellent tackifying, shear-improving and filtrate-reducing effects under high-temperature and high-salt conditions, improves the rheological property and the filtrate-reducing property of drilling fluid, and ensures safe and efficient drilling of a deep well.

According to the amido-modified polyanionic cellulose filtrate reducer and the preparation method thereof, amido functional groups are introduced into a molecular structure, and a final product is modified into a zwitterionic type product, so that the temperature resistance of the amido-modified polyanionic cellulose filtrate reducer can be improved, and the temperature resistance of the product can reach 160 DEG C. Besides, due to introduction of amido and a long alkylene oxide chain in the molecular structure, the adsorption capacity and the film-forming lubricating capacity of a modified product can be improved, the filtrate loss reduction performance of the product is remarkably improved, the filtrate loss reduction performance at the normal temperature is improved by at least 30% compared with that of conventional polyanionic cellulose, and the filtrate loss after aging at 160 DEG C is smaller than 13 mL. The amido modified polyanionic cellulose filtrate reducer provided by the invention is good in temperature resistance and obvious in filtrate reduction effect.

The invention discloses a 13-hydroxyglucopiericidin A heterologous expression engineering strain, a construction method of the 13-hydroxyglucopiericidin A heterologous expression engineering strain and an application of the 13-hydroxyglucopiericidin A heterologous expression engineering strain. According to the engineering strain, Streptomyces avicularis SUKA17 is taken as a host, and heterologous synthesis of the chalicimycin glycoside, especially 13OH-GPA, is realized by introducing a biological synthesis gene cluster of the chalicimycin glycoside; the bacterial strain is classified and named as Streptomyces sp. SK-SA23E, and the preservation number of the bacterial strain is GDMCC NO.67271. The invention further discloses a preparation method of the bacterial strain. According to the invention, the efficient heterologous expression of the biological synthesis gene cluster of the chalcone glycoside is realized for the first time, the yield of the 13OH-GPA in the engineering strain is increased by 2.8 times compared with that of the original production strain, the relative content of the 13OH-GPA in the chalcone glycoside is increased to 48.1% from 23.6%, and the method is suitable for large-scale preparation of the 13OH-GPA.

The application discloses a process for improving the quality of palm kernel meal feed by enzyme and bacteria synergistic fermentation, and belongs to the field of feed biotechnology.The process is characterized by the following steps: crushing and screening palm kernel meal, mixing the palm kernel meal with 20% bran and 4% ammonium sulfate as dry matter to obtain a basic substrate;compounding bacillus subtilis, aspergillus niger and saccharomyces cerevisiae at a ratio of 1:1:1 of viable bacterial number, adding 0.67% mannanase and 0.3% xylanase to prepare a bacteria-enzyme mixed solution, inoculating the bacteria-enzyme mixed solution at a 10% inoculation amount, adjusting the moisture content to 50% and the pH to 6.4, and then sealing and storing the bacteria-enzyme mixed solution for use after 4 days of fermentation at 33.5 DEG C; and the bacteria-enzyme mixed solution can be stored for a long time.The process can efficiently degrade crude fiber and anti-nutritional factors, improve the protein content and the digestibility, is stable and easy to mass-produce, can replace part of corn and soybean meal in the daily ration of ruminants, and realizes high-value utilization of palm kernel meal.

The invention belongs to the technical field of microbial fermentation, and particularly relates to screening and application of lactic acid bacteria with high yield of gamma-aminobutyric acid. According to the method disclosed by the invention, the high-content gamma-aminobutyric acid is produced by utilizing the newly screened lactobacillus mucus fermentation SJM8. By optimizing the fermentation process, the content of gamma-aminobutyric acid produced by SJM8 is increased by 2.5 times compared with that before optimization, the fermentation period is shortened, and the energy consumption and the production cost are reduced while the yield of gamma-aminobutyric acid is increased.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a compound microbial agent and application thereof in fermentation of high-acidity Hmong's red sour soup. The red sour soup with high acidity and low nitrite content is produced by fermenting tomatoes, hot peppers and other auxiliary materials by using a compound microbial agent prepared from newly screened lactobacillus plantarum MX14 and pichia membranaefaciens PY33, and mixing and homogenizing the tomatoes, the hot peppers and the other auxiliary materials. Compared with naturally fermented sour soup, the preparation method has the advantages that the fermentation period is short, the prepared red sour soup has higher titratable acid content, lower pH and lower nitrite content, the fermentation period of rapid acidification of the red sour soup is shortened, nitrite in the red sour soup is reduced, and the safety of products is improved.

The invention discloses a preparation method of an environment-friendly salt-resistant and high-temperature-resistant filtrate reducer for a long open hole section. The preparation method specifically comprises the following steps: step 1, preparing a first mixed solution; step 2, preparing dispersion liquid; step 3, mixing the first mixed solution and the dispersion solution, and then shearing for 1-2 hours to obtain a shearing solution; step 4, adding the shear fluid into a reaction kettle, introducing nitrogen into the reaction kettle, discharging air in the reaction kettle, keeping the temperature at 70 DEG C, stirring for 10 minutes while keeping the temperature, adding an initiator, and stirring for 10-12 hours to obtain a reaction liquid; step 5, adding the reaction liquid into isopropanol according to a volume ratio of 1: 1.5, stirring for 40 minutes, standing for 2 hours, and filtering to obtain a reaction material; and step 6, washing the reaction material with absolute ethyl alcohol and methanol for 10 minutes respectively, and then drying to obtain the environment-friendly salt-resistant and high-temperature-resistant filtrate reducer for the long open hole section. The problem that an existing filtrate loss agent is poor in filtrate loss reduction performance in a long open hole section is solved.

The present invention belongs to the technical field of microbial fermentation, and particularly relates to a composite microbial agent and its application in preparing white sour soup. The present invention uses a composite microbial agent prepared by newly screened Lactobacillus paracasei MY6 and Pichia pastoris PY4 to ferment glutinous rice homogenate to produce white sour soup with high acidity and low nitrite content. Compared with naturally fermented sour soup, the fermentation cycle prepared by the preparation method of the present invention is short, and the prepared white sour soup has a higher titratable acid content, a lower pH and a lower nitrite content, which not only shortens the fermentation cycle of rapid acidification of white sour soup, but also reduces the nitrite in the white sour soup, thereby improving the safety of the product.

The invention discloses a leavening agent containing leuconostoc mesenteroides subsp. Mesenteroides and application of the leavening agent in fermentation of dendrobium milk, and belongs to the technical field of fermentation engineering. The fermentation agent is prepared from leuconostoc mesenteroides subsp. Mesenteroides JMCC0041, bifidobacterium animalis subsp. Lactis and lactobacillus mucus fermentation according to the viable count ratio of 1: 1: (1-5). When the fermentation agent is used for fermenting dendrobium nobile milk, the content of polysaccharide, alkaloid and small molecule peptide in a fermentation product can be remarkably increased, and the effect of protecting gastric mucosa is achieved by inhibiting the activity of helicobacter pylori and relieving inflammatory reaction. The fermentation process is simple, convenient and rapid, does not need segmented fermentation, and is suitable for preparing functional products through fermentation.

The application belongs to the technical field of utilization of waste biomass by microorganisms, and specifically discloses an abnormal williamsonia strain Y8 and application thereof in beer dreg fermentation. The preservation number of the strain is CCTCC NO: M 2026579. The strain has good tolerance to extreme chemical environments such as high salt, high sugar, strong acid and high-concentration ethanol; can ferment polysaccharides and monosaccharides; under the condition of rich nutrition, the content of cell protein can reach 57%; can adapt to the liquid physicochemical environment of beer dregs and effectively utilize beer dregs to grow. With the aid of enzyme preparation composed of beta-glucanase and xylanase, the efficiency of the strain in utilizing beer dregs to grow is significantly improved, and after 72 h of fermentation, the fermentation liquor with viable cell count of the strain ≥1.8×10 9 CFU / mL is obtained, and the prepared fermentation feed is effectively enriched with total phenols and improved in antioxidant performance. The application is green, mild and efficient, and has excellent application prospect.

The present invention relates to the technical field of oil drilling fluid, and in particular to a lubricant additive and preparation method, lubricant, water-based drilling fluid and preparation method, the lubricant additive comprises the following raw materials: 6 parts of taurine, 30-60 parts of 1-bromohexadecane, 8.5-15 parts of potassium hydroxide, 0.5-0.9 parts of potassium iodide, 30-60 parts of thionyl chloride, 2-4 parts of N,N-dimethylformamide, 7-17 parts of ammonia water and 30 parts of chloroform, the lubricant additive uses sulfonic acid amine group as active adsorption group, and can be stably adsorbed on drill string, casing and mud cake. At the same time, it has two C16 flexible chains, can form a dense lubricating film, and efficiently reduce friction resistance.

The invention relates to the technical field of microorganisms, in particular to Enterobacter hormaechei C4 and application thereof, the Enterobacter hormaechei C4 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, the preservation date is January 13, 2023, and the preservation number is CGMCC No.26480. The enterobacter hormaechei C4 provided by the invention can generate more flavor substances by utilizing cellulose, starch and other components in the tobacco in the fermentation process, so that the aroma quality, aroma quantity, concentration, irritation, aftertaste and sweetness of the tobacco are improved, and the tobacco has more excellent sensory quality.

The invention belongs to the technical field of drilling fluid, and particularly relates to supersaturated brine drilling fluid as well as a preparation method and application thereof. The supersaturated salt water drilling fluid comprises water, a pH regulator, a tackifier, a filtrate reducer, a dispersant, a salt inhibitor, a powdery soluble salt and a soluble salt. The supersaturated salt water drilling fluid has a supersaturated functional characteristic, supersaturated salt suspends in the drilling fluid in the form of particles, dissolution of a salt-gypsum layer is prevented, salt particles have a temporary plugging function, reservoir damage can be prevented, and the supersaturated salt water drilling fluid has a very good reservoir protection effect.

The invention discloses a method for preparing micro-endosperm corn plant-based yoghourt through enzymolysis and bitterness removal and a product, and belongs to the technical field of fermentation. According to the invention, flavourzyme, glutamine transaminase and alpha-amylase are combined to carry out enzymolysis treatment on a micro-endosperm corn fermentation base, and hydrophobic amino acids at the tail ends of proteins are removed by directionally hydrolyzing the proteins before fermentation, so that the bitter taste of the yoghourt product is effectively reduced; more importantly, the stability of a protein gel network structure is enhanced through a synergistic enzymolysis effect, energy is provided for subsequent strain fermentation, and the purposes of improving the fermentability of the strain and improving the yield of the microendosperm corn plant-based yoghurt are achieved by combining the subsequent optimization of a preparation process of the microendosperm corn plant-based yoghurt and determining the optimal sugar addition amount, the fermentation agent inoculation amount and the fermentation time. The purpose of improving the quality and the taste of the yoghourt is achieved, the brand-new high-quality micro-endosperm corn plant-based yoghourt is prepared, industrialization of micro-endosperm corn is promoted, and important data support is provided for research of the preparation technology of the plant yoghourt in China.

This invention discloses a method for preparing liquid fermented tea that enhances the floral and fruity aroma of tea leaves, belonging to the field of fermented tea technology. This invention uses Pichia pastoris (a type of yeast). Pichia cactophila FL2 fermented tea liquor, after fermentation, shows an increase of more than 11.59 times in the content of floral and fruity aroma compounds such as terpinene, an increase of 86.58 times in the content of phenylethanol, an increase of 82.83 times in the content of phenethyl acetate, and an increase of 369.26 μg / mL in the content of 1-butanol and o-cymene from 0. In addition, the increases in cymene, α-terpineol, and nerolidol reached 5.41, 13.54, and 3.34 times, respectively. The interaction of various aroma components gives the tea liquor a unique "lemon and citrus fruity aroma" flavor, while also containing sweet and sour aroma compounds. It has a good flavor and is of industrial promotion value.