181results about How to "Improve fermentation performance" patented technology

The invention discloses a saccharomyces cerevisiae strain with high yield of ester and low yield of higher alcohol as well as a building method of the saccharomyces cerevisiae strain, and belongs to the technical field of bioengineering. According to the building method provided by the invention, through completely knocking out an amino acid transaminase gene BAT2 and an ester hydrolase gene IAH1 in an original strain, and selecting a strong promoter PGK1 over-expression alcohol acetyltransferase I gene ATF1 at the same time, the saccharomyces cerevisiae strain with high yield of ester and low yield of higher alcohol is obtained. Compared with a parent strain, other fermentation performances of built recombinant bacteria are not affected, the total quantity of acetic acid ester is obviously increased and reaches 1303.6mg/L, wherein the content of ethyl acetate is 52 times that of the original strain, isoamyl acetate is increased to 73.7mg/L, the content of main higher alcohol is 151.8mg/L and is reduced by 61.4 percent in comparison with that of the original strain. By using the saccharomyces cerevisiae, ester yield is significantly increased while the higher alcohol yield is reduced, the higher requirements of white spirit related fields on yeast are met and the application prospect is wide.

The invention provides newly-separated Lactobacillus plantarum HEW-A490. The Lactobacillus plantarum HEW-A490 has the advantages that the Lactobacillus plantarum HEW-A490 is evident in probiotic performance and stress resistance, the probiotics prepared by the Lactobacillus plantarum HEW-A490 can fast activate and proliferate after entering the intestines of animals and can form a beneficial dominant bacterial community to maintain intestinal track balance, animal feed utilization efficiency can be increased, cost can be saved, the steady state of the digestive tract internal environments in the animals is improved, nutrition absorption is promoted, animal growth is promoted, economic benefits are increased, the Lactobacillus plantarum HEW-A490 is promising in application prospect, and the like.

The invention discloses enterococcus faecalis HEW-A131 which is high in thermal resistance, wide in acid-alkali resistance range, high in stress resistance and relatively high in probiotic property. The strain is preserved in the China General Microbiological Culture Collection Center on June 17th, 2014, the preservation serial number is CGMCC NO.9353, and the classification name is enterococcus faecalis. The enterococcus faecalis HEW-A131 has excellent microorganism characteristics, remarkable probiotic property and stress resistance and super high fermentation property, not only is the utilization efficiency of feed of animals increased, but also the cost is reduced, moreover the stability of the environment inside the alimentary canals of animals is greatly improved, the adsorption and utilization of nutrition are promoted, the animal growth is promoted, the production performance, immunity and breeding property of the animals are remarkably improved, the production cost is lowered, and the economic benefits are increased.

The invention discloses a preparation method for a high-temperature delay type organic zirconium cross-linking agent. The preparation method comprises the following steps of: sufficiently dissolving inorganic zirconium salt in water and adding a proper amount of organic acid; under the catalytic action of NaOH, controlling the pH value of the system and raising the temperature and reacting to obtain organic acid-zirconium prepolymer; keeping the temperature constant, adding a ligand and a cross-linking shielding agent into the organic acid-zirconium-prepolymer to perform reaction; continuously keeping the temperature constant, adding polyalcohol and a high-temperature stabilizing agent, adjusting the pH value and reacting at constant temperature; removing the high-temperature stabilizing agent residue after reaction is finished so as to obtain the modified clarified transparent high-temperature delay type cross-linking agent. The aqueous fracturing fluid prepared by the invention has good shear resistance, good sand carrying property and wide application prospect, greatly enhances the heat resistance property, reduces the use cost and relieves damage to the stratum environment.

The invention discloses a beneficial microorganism antibiont-free feed and a preparation method of the beneficial microorganism antibiont-free feed. The preparation method comprises the following steps of: preparing a Lactobacillus acidophilus bacteria solution, a lactobacillus plantarum bacteria solution, a Saccharomyces cerevisiae bacteria solution and a Bacillus licheniformis bacteria solution respectively by carrying out secondary fermentation; then mixing the Bacillus licheniformis bacteria solution with cottonseed meal to carry out aerobic fermentation; mixing the fermented cottonseed meal, corn flour, beer slag and premixes uniformly, and adding a compound bacteria solution prepared from the Lactobacillus acidophilus bacteria solution, the lactobacillus plantarum bacteria solution and the Saccharomyces cerevisiae bacteria solution, mixing uniformly, carrying out constant temperature fermentation, thus obtaining the antibiont-free feed. The antibiont-free feed disclosed by the invention is not added with antibiotic medicines, is high in the content of the beneficial microorganism, has good attractant performance, palatability, and high nutrition digestion and absorption rate, increases the feed intake of animals, promotes the animal growth, enhances the level of animal health, and improves animal immune resistance to disease. The antibiont-free feed is applicable to breeding of antibiont-free livestock and poultry products, so that the breeding cost can be lowered, the economic efficiency is enhanced and the healthy development of aquaculture is promoted.

The invention relates to sulfonic acid modified carboxymethyl hydroxypropyl guar gum, and a preparation method and application thereof. The structural formula of the sulfonic acid modified carboxymethyl hydroxypropyl guar gum is shown in the specification, wherein R is H, and n is 0, 1, 2 or 3; or R is OH, and n is 0-6. The preparation method comprises the following steps: adding carboxymethyl hydroxypropyl guar gum into a reaction vessel, and adding an alcohol water solution; in a nitrogen atmosphere, dropwisely adding a sodium hydroxide water solution, heating to 65-80 DEG C while stirring, dropwisely adding a sulfonating agent, and reacting for 3-10 hours; and regulating the pH value of the solution to 7 with acid, filtering, collecting solids, and drying to obtain light yellow powder, namely the sulfonic acid modified carboxymethyl hydroxypropyl guar gum. The prepared sulfonic acid modified carboxymethyl hydroxypropyl guar gum is high in thickening efficiency, low in surface/interface tension, easy to realize viscosity break and beneficial to flowback.

The invention relates to a plugging-type filtrate reducer for water-based drilling fluid and a preparation method thereof. The filtrate reducer can be selected in accordance with different well section temperatures and strata characteristics, and comprises polymer modified oxidized asphalt (PMA), a modifying agent and an auxiliary filtrate reducer; and the filtrate reducer series samples can be prepared by a simple surface modification and dry-blending process. The sample disclosed by the invention is excellent in performance of reducing filtrate of the drilling liquid with the reducing rate of 80%, a dense and tough mud cake is formed, and the crack and fracture of the strata can be plugged in real time based on high-temperature softening and deforming characteristic of the asphalt; an appropriate product formula can be selected depending on the applied strata characteristics and well section temperatures; and the plugging-type filtrate reducer for the water-based drilling fluid is flexile to use, simple in production process, moderate in condition, high in production efficiency, low in cost, free from waste and dust, and low in harm on the body of an operator.

The invention relates to a temporary plugging steering acidification method applicable to a sandstone reservoir, which belongs to the technical field of yield increasing and injection increasing of oil and gas fields. By adopting pad-acid and synergistic liquid, the temporary plugging of a high permeable layer of the non-homogenous sandstone reservoir can be realized, and the temporary plugging performance is good, so that the subsequently injected main body acid liquid can be successfully diverted to enter a low permeable layer and to acidify the low permeable layer. By adopting the acidification method, the sandstone reservoir can be effectively and homogenously acidified, and the non-homogeneity of the reservoir can be improved.

The invention discloses a high temperature yeast (Saccharomyces cerevisiae) CGMCC No.7513 for producing msalais through fermentation and msalais obtained by a preparation method through fermentation by utilizing the strain. By virtue of analysis at different temperatures, sugar degrees, alcohol contents and low nitrogen tolerances, metabolism property determination, detection of wine production capacity, flocculability, autolysis, H2S production capacity and biogenic amine production characteristic, determination of pectinase activity and determination test on activity of beta-glucosaccharase, the high temperature msalais yeast strain with excellent brewing performance is screened, and the high temperature msalais yeast strain is widely applied to the fields of light industry production and the like.

The invention discloses an efficient traceless gene knockout method for saccharomyces cerevisiae. By optimizing the forward homologous sequence, galactose concentration and galactose induction time ina traceless gene knockout system, the probability of homologous recombination of the second step reaches 6.86*10<-4>. Haploids alpha 5 of saccharomyces cerevisiae AY15 serves as starting strains, BAT2 genes serves as the target gene, efficient and traceless knockout of wild saccharomyces cerevisiae genes BAT2 is achieved, through a liquor fermentation experiment, the contents of n-propanol, isobutanol and isoamyl alcohol of the modified strains are reduced by 20.32%, 47.85% and 23.14% respectively compared with these of parent strains, and the aim of low yield of higher alcohols is achieved.The method can be widely used in genetic modification of yeast and other microorganisms, because obtained mutants have no exogenous genes left and thus can be safely used in industrial production, anda useful reference is provided for gene knockout directly conducted on industrial strains.

A method for enhancing flavor of low-salt soy sauce obtained by solid state fermentation, which solves the problems of the prior low-salt soy sauce obtained by solid state fermentation, including poor flavor, low content of total esters and no ester flavor. The method comprises (1) immobilizing yeast cells with magnetic chitosan microspheres as a carrier to obtain immobilized yeast cells; and (2) inoculating the immobilized yeast cells in soy sauce mash, and fermenting to obtain low-salt soy sauce with enhanced flavor. In the obtained low-salt soy sauce with enhanced ester flavor, the content of total esters is increased by 15-20 wt%, the content of ethyl acetate is increased by 11-20 wt%, and the content of 4-ethyl guaiacol is increased by 12-15 wt%.

The invention relates to the field of drilling fluids for petroleum, natural gas and natural gas hydrate drilling and discloses a water-based clay-free drilling fluid and application of the drilling fluid to polar region frozen soil stratigraphical drilling. The drilling fluid comprises an ultralow-temperature base liquid, a tackifier, a filtrate reducer, a blocking agent, a lubricating agent andwater, wherein the ultralow-temperature base liquid is composed of inorganic salt and alcohol, the solidifying point of the ultralow-temperature base liquid is lower than or equal to -28 DEG C, and the heat conduction coefficient is smaller than 0.5W/m.K. The water-based clay-free drilling fluid has good rheological properties, filtration properties, low heat conduction characteristics, well wallstability and environment-friendliness even under the condition of -25 DEG C and can be applied to polar region frozen soil stratigraphical drilling.

The invention discloses a Saccharomyces cerevisiae strain low in higher alcohol yield and a building method thereof and belongs to the technical field of bioengineering. The Saccharomyces cerevisiae strain is built by regulating the biotin synthesis route of Saccharomyces cerevisiae and knocking out the gene complete sequence of precursor substances, capable of promoting Saccharomyces cerevisiae biotin synthesis, in an original Saccharomyces cerevisiae strain, and the specific genes are a BIO3 gene for encoding 7,8-diamino-pelargonic acid aminotransferase and a BIO5 gene for encoding 8-amino-7-ethyl oxopelargonate carrier protein. After the Saccharomyces cerevisiae strain is fermented in a fermentation culture medium using wheat as the raw material, and the total higher alcohol generationquantity of the Saccharomyces cerevisiae strain is lowered by 16.85% and 23.55% as compared with that of the parent strain; the strain is good in fermentation performance and growth performance, and the condition that the growth performance of the recombinant strain is affected or other conditions can be avoided.

The invention discloses saccharomyces cerevisiae CGMCC No. 3027 for producing pomegranate fruit wine and the pomegranate fruit wine fermented and brewed by utilizing same. A plurality of strains of yeast are separated and purified through different approaches, saccharomyces cerevisiae with excellent wind-making performance of pomegranate fruit wine is selected out through tests of resistance against alcohol, SO2, high-low temperature as well as high glucose and an abricotine fermentation test, and the fermentation technology of pomegranate fruit wine is determined; and clear and transparent pomegranate fruit wine with cherry color, typical pomegranate fruit fragrance, alcohol degree of 6.6-9.7 percent by V/V, total sugar of 180g/L, total acid of 4g/L and good alcohol flavor is brewed through closed fermentation of main fermentation at the temperature of 25 DEG C and post fermentation at the control temperature of 18-22 DEG C. The pomegranate fruit wine has full body, fineness, mellowness, good harmony and moderate acidity, and the invention is widely applied to the field of the industrial production of the pomegranate fruit wine.

The invention belongs to the technical field of microorganisms, and particularly relates to debaryomyces hansenii with good fermentation performance and an aroma production function and a screening method thereof. The strain is named as Debaryomyces hansenii SH4, which is preserved in the China General Microbiological Culture Collection Center on May 12, 2021, and the preservation number of the Debaryomyces hansenii SH4 is CGMCC No.22507. After the debaryomyces hansenii SH4 is inoculated into a fermented sausage simulation system and fermented for 12 days, the contents of aldehydes, acids, alcohols and esters are obviously higher than those of an uninoculated control group (P is greater than 0.05). The fat taste of the simulation system is reduced, the fermentation taste, the wine fragrance, the fruit fragrance and the flower fragrance are increased, and good quality as an aroma production leavening agent is shown. The debaryomyces hansenii provided by the invention is applied to the air-dried sausages and has important significance in improving the fragrance of products and increasing the strength and complexity of the fragrance.

The invention discloses a filtration reducing agent for a drilling fluid and a preparation method thereof. The filtration reducing agent comprises the components in parts by weight: 200-250 parts of phenol, 50-60 parts of sodium chloroacetate, 50-60 parts of epoxy chloropropane, 380-430 parts of formaldehyde, 80-100 parts of sodium metabisulfite, 80-90 parts of anhydrous sodium sulfate, 65-78 parts of sodium hydroxide, 60-70 parts of trimethylamine, 36-45 of sodium 2-chloroethanesulfonate, 25-35 parts of carboxymethyl cellulose, 30-40 parts of acrylic amine, 28-40 parts of vinyl acetate, 30-36 parts of dimethyl diallyl ammonium chloride, and 800-1000 parts of water. The preparation method comprises the steps of firstly dissolving sodium hydroxide in water, adding phenol and sodium chloroacetate, carrying out a reaction, then adding epoxy chloropropane and trimethylamine, continuing to carry out a reaction, after the reaction, adding other components, successively carrying out reactions, and carrying out spray drying on the finally-obtained mixture to obtain the filtration reducing agent product.