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Lactobacillus plantarum HEW-A490 and application thereof

A HEW-A490, Lactobacillus plantarum technology, applied in the application, bacteria, microorganisms and other directions, can solve the problems of heat resistance, acid and alkali resistance, bacteriostatic performance and fermentation performance of Lactobacillus plantarum, which cannot meet the needs of industrialization. , to achieve the effect of regulating animal intestinal microbial balance, improving animal production performance and improving nutritional value

Active Publication Date: 2017-02-15
BEIJING HESWOF BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing Lactobacillus plantarum is not ideal in heat resistance, acid and alkali resistance, antibacterial performance and fermentation performance, and cannot meet the needs of growing industrialization, especially from the current goals of the feed industry and animal husbandry.

Method used

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  • Lactobacillus plantarum HEW-A490 and application thereof
  • Lactobacillus plantarum HEW-A490 and application thereof
  • Lactobacillus plantarum HEW-A490 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Isolation, screening, identification and preservation of Lactobacillus plantarum HEW-A490

[0052] 1. Isolation and purification of Lactobacillus plantarum

[0053] Aseptically take the intestinal contents of healthy chickens and carry out enrichment culture in 100mL MRS liquid medium, draw 0.5mL enrichment culture solution into 4.5mL sterile saline, carry out 10-fold gradient dilution in turn, select the appropriate dilution to take Spread 0.1mL of the diluted solution on the MRS+ calcium carbonate medium plate, spread evenly, incubate upside down at 37°C for 24-48h, pick out the colonies of different shapes that produce calcium-dissolving circles and inoculate them for pure culture, and store them at 4°C for later use.

[0054] 2. Observation of colony morphology

[0055] On the MRS plate, the colony is milky white, round, with neat edges, indicating that it is moist and smooth and easy to pick up.

[0056] 3. Screening of excellent Lactobacillus plantarum...

Embodiment 2

[0087] The preparation of embodiment 2 Lactobacillus plantarum HEW-A490 probiotics

[0088] Lactobacillus plantarum HEW-A490 (preservation number is CGMCC No.12554) was prepared according to the method of step 3 of Example 1 to prepare HEW-A490 fermentation broth, and the fermentation broth was centrifuged at 10000r / m for 30min to obtain Lactobacillus plantarum HEW-A490 active sludge , the Lactobacillus plantarum HEW-A490 active bacteria slime and protective agent were mixed evenly in a weight ratio of 1:0.8, and water was added to adjust the bacterial concentration to 10 11 CFU / g, made into microcapsule wet powder, then mixed evenly with the coating agent solution, put into a spray granulation dryer, dried and granulated to obtain Lactobacillus plantarum HEW-A490 microecological preparation, the effective number of viable bacteria reached 1 ×10 11 CFU / g.

[0089] The protective agent is 8.5% trehalose, 2.4% inulin, 11% skimmed milk powder, 14% gelatinized modified starch, 2...

Embodiment 3

[0091] Example 3 Research on the Application of Lactobacillus plantarum HEW-A490 Microecological Preparation Fermented Feed

[0092] Take 20kg of ordinary soybean meal, adjust the humidity to 60%, after sterilization, add 0.2g of the Lactobacillus plantarum HEW-A490 microecological preparation prepared in Example 2, activate it with sterilized physiological saline for 15-30min, inoculate it into the soybean meal, mix and stir evenly , packed into barrels and sealed, fermented at 37°C for 3 days, dried and crushed, sieved (80 mesh), and the content of anti-nutritional factors before and after fermentation of soybean meal was detected. The results are shown in Table 4. Adding Lactobacillus plantarum HEW-A490 can significantly reduce the content of anti-nutritional factors such as sucrose, stachyose, raffinose, tannin, glycinin and trypsin inhibitor in soybean meal, improve the nutritional value of soybean meal and improve the flavor , which is conducive to the feeding of fermen...

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Abstract

The invention provides newly-separated Lactobacillus plantarum HEW-A490. The Lactobacillus plantarum HEW-A490 has the advantages that the Lactobacillus plantarum HEW-A490 is evident in probiotic performance and stress resistance, the probiotics prepared by the Lactobacillus plantarum HEW-A490 can fast activate and proliferate after entering the intestines of animals and can form a beneficial dominant bacterial community to maintain intestinal track balance, animal feed utilization efficiency can be increased, cost can be saved, the steady state of the digestive tract internal environments in the animals is improved, nutrition absorption is promoted, animal growth is promoted, economic benefits are increased, the Lactobacillus plantarum HEW-A490 is promising in application prospect, and the like.

Description

technical field [0001] The invention relates to probiotics for animal husbandry, in particular to a strain of Lactobacillus plantarum HEW-A490 and its application. Background technique [0002] Lactobacillus plantarum (Lactobacillus plantarum) is a normal intestinal flora of humans and animals, widely present in traditional fermented foods. Many studies have proved that Lactobacillus plantarum can inhibit the growth and reproduction of pathogenic bacteria, promote the body's digestion and absorption of nutrients, and improve the body's Immunity and other aspects have important biological functions. Lactobacillus plantarum inhibits the growth of pathogenic bacteria by competing with the ability of pathogenic bacteria to limit nutrients, regulates the composition of intestinal microecology, and forms a biological barrier. At the same time, through the effects of the metabolites lactic acid, bacteriocin, and diacetyl produced by its metabolism on other bacteria, adjust the rel...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18C12R1/25
CPCC12N1/20C12N1/205C12R2001/25
Inventor 李雪平
Owner BEIJING HESWOF BIOTECH CO LTD
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