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Beneficial microorganism antibiont-free feed and preparation method thereof

An anti-biological and microbial technology, applied in animal feed, animal feed, application, etc., can solve problems such as scientific selection and compounding of strains, and achieve the effect of promoting healthy development, easy assembly line operation, and improving animal health.

Inactive Publication Date: 2013-05-15
GUANGXI FULAIKANG FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems in the production of antibiotic-free feed, such as the optimization of key fermentation processes and the scientific selection and compounding of strains

Method used

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  • Beneficial microorganism antibiont-free feed and preparation method thereof
  • Beneficial microorganism antibiont-free feed and preparation method thereof
  • Beneficial microorganism antibiont-free feed and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Secondary fermentation preparation of beneficial microorganism liquid

[0027] Preparation of Lactobacillus acidophilus bacterial liquid: primary bacterial liquid - inoculate the activated strains in 500ml sterile MRS medium, the inoculum amount is 2% by volume, and culture in an anaerobic incubator at 37°C for 24 hours , the number of viable bacteria ≥ 1 × 10 9 CFU / ml; secondary bacterial liquid——put the primary bacterial liquid into 100L sterile MRS medium (in a fermentation tank) according to aseptic procedures, with an inoculation amount of 2%, anaerobic culture at a constant temperature of 37°C for 24 hours, every Stir the medium for 2 hours for 1 minute, the number of viable bacteria ≥ 1×10 9 CFU / ml;

[0028] Preparation of Lactobacillus plantarum liquid: primary bacterial liquid—inoculate the activated strain into 500ml of sterile MRS medium, the inoculum amount is 3% by volume, and culture it in an anaerobic incubator at 37°C for 36 hours. The number of viabl...

Embodiment 2

[0043] In step , 2 kg of Bacillus licheniformis bacterial liquid, fermented cottonseed meal small peptide content is 8%; According to the weight ratio of 3:3:1) 7kg, no antibiotic feed, small peptide content of 2.1% (calculated on a dry basis), pH value of 4.7. In addition, others are basically the same as in Embodiment 1.

[0044] Feeding test: select 80 lean-meat pigs with an initial body weight of 45 ± 0.68kg, divide them into test groups and control groups, and the test groups are fed with the antibiotic-free feed made in Example 2 (the amount of feed is converted to 88% by dry matter content) ), the control group was fed conventional diets containing antibiotics (without fermentation treatment), the pre-feeding period was 5 days, and the formal test period was 25 days. The results are shown in Table 2.

[0045] Table 2 embodiment 2 no antibiotic feed feeding test result

[0046]

Embodiment 3

[0048] In step , 3.5kg of Bacillus licheniformis bacterial liquid, the small peptide content of fermented cottonseed meal is 9.5%; Liquid (2:1:3 by weight ratio) 6kg, no antibiotic feed, small peptide content of 2.8% (calculated on a dry basis), pH value of 5.2. In addition, others are basically the same as in Embodiment 1.

[0049] Feeding test: select 100 lean-meat pigs with an initial body weight of 46 ± 0.95kg, divide them into test groups and control groups, and the test groups are fed with the antibiotic-free feed made in Example 3 (the amount of feed is converted to 88% by dry matter content) ), the control group was fed conventional diets containing antibiotics (without fermentation treatment), the pre-feeding period was 5 days, and the formal test period was 25 days. The results are shown in Table 3.

[0050] Table 3 embodiment 3 no antibiotic feed feeding test result

[0051]

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Abstract

The invention discloses a beneficial microorganism antibiont-free feed and a preparation method of the beneficial microorganism antibiont-free feed. The preparation method comprises the following steps of: preparing a Lactobacillus acidophilus bacteria solution, a lactobacillus plantarum bacteria solution, a Saccharomyces cerevisiae bacteria solution and a Bacillus licheniformis bacteria solution respectively by carrying out secondary fermentation; then mixing the Bacillus licheniformis bacteria solution with cottonseed meal to carry out aerobic fermentation; mixing the fermented cottonseed meal, corn flour, beer slag and premixes uniformly, and adding a compound bacteria solution prepared from the Lactobacillus acidophilus bacteria solution, the lactobacillus plantarum bacteria solution and the Saccharomyces cerevisiae bacteria solution, mixing uniformly, carrying out constant temperature fermentation, thus obtaining the antibiont-free feed. The antibiont-free feed disclosed by the invention is not added with antibiotic medicines, is high in the content of the beneficial microorganism, has good attractant performance, palatability, and high nutrition digestion and absorption rate, increases the feed intake of animals, promotes the animal growth, enhances the level of animal health, and improves animal immune resistance to disease. The antibiont-free feed is applicable to breeding of antibiont-free livestock and poultry products, so that the breeding cost can be lowered, the economic efficiency is enhanced and the healthy development of aquaculture is promoted.

Description

technical field [0001] The invention belongs to the field of microbial fermented feed, and in particular relates to a beneficial microbial antibiotic-free feed and a preparation method thereof. Background technique [0002] The modern breeding industry tends to be large-scale and intensive, and the use of antibiotics has become a key link to ensure the development of animal husbandry. However, due to unscientific drug use and drug abuse in the breeding industry, the drug residues in livestock and poultry meat products exceed the standard, and induce the aggravation of drug resistance of pathogenic bacteria. The residual drugs will affect the health of consumers through the food chain; Antibiotic drugs will change the inherent microbial structure of the soil and disrupt the ecological balance of nature. In recent years, with the frequent exposure of incidents of excessive drug residues in meat products, animal husbandry workers have begun to consider the coordinated developm...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/06A23K1/16A23K10/38
CPCY02P60/87
Inventor 夏宁莫武威
Owner GUANGXI FULAIKANG FEED
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