1 results about "Phosphomannose Isomerase" patented technology

The invention discloses a platycodon grandiflorum phosphomannose isomerase gene PgPMI as well as an encoded product and application thereof, and belongs to the technical field of platycodon grandiflorum genes. According to the invention, the platycodon grandiflorum phosphomannose isomerase gene PgPMI is cloned from platycodon grandiflorum, and the nucleotide sequence of the platycodon grandiflorum phosphomannose isomerase gene PgPMI is shown as SEQ ID NO. 1. According to the invention, the expression of the PgPMI gene in escherichia coli is successfully realized, and an enzymatic experiment verifies that the PgPMI has catalytic activity, so that mannose-6-phosphoric acid and fructose-6-phosphoric acid can be mutually converted, and a foundation is laid for revealing biosynthesis of platycodon grandiflorum polysaccharide. In addition, the platycodon grandiflorum phosphomannose isomerase can also be applied to biosynthesis pathways of plant ascorbic acid, cell walls, glycosylated proteins, lipoproteins, glycosyl phosphate inositol ankyrin and the like.