35 results about "Phosphomannose Isomerase" patented technology

The object is to develop a bacterium capable of fermenting glucose, mannose and xylose simultaneously, which can ferment a saccharified solution of a cellulose-type or lignocellulose-type biomass resource to produce ethanol, and to construct an energy-saving high-efficiency bioethanol conversion process. Thus, disclosed is Zymomonas mobilis bacterium which is prepared by integrating a gene encoding a phosphomannose isomerase derived from Escharichia coli into a levansucrase gene located on the chromosome by the double cross-over by means of a homologous recombsination method, and then introducing recombinant DNA prepared by binding a DNA fragment containing genes encoding a xylose isomerase, a xylulokinase, a transaldolase and a transketolase, respectively, all derived from Escherichia coli to a vector. Also disclosed is a method for producing ethanol by continuously fermenting a saccharified solution of a cellulose-type biomass resource in a system on which the Zymomonas mobilis bacterium is immobilized.

The invention provides phosphomannose isomerase from chlorella variabilis. The phosphomannose isomerase from the chlorella variabilis is named as ChloPMI. The invention further provides a prokaryotic expression vector containing the ChloPMI, and the prokaryotic expression vector can be used for identifying the proteometabolism mannose activity of the ChloPMI. The invention further provides an expression cassette containing the ChloPMI, a plant expression vector, application of the expression cassette and the application of the plant expression vector on the aspect of plant genetic transformation. The plant expression vector constructed by utilizing the ChloPMI uses mannose as a screening reagent and successfully achieves conversion of rice cells. The phosphomannose isomerase is successfully separated and cloned from the chlorella variabilis, due to the facts that the chlorella variabilis derives from the chlorella variabilis, is environmentally friendly and has no potential risks to the human body, application and popularization of transgenic products are facilitated, and doubts about transgenosis are cleared.

The invention provides a method of effectively introducing exogenous genes into rice cells by utilizing PMI (phosphomannose isomerase) selective marker, a transformation system, as well as a preparation method of genetically modified rice by utilizing the method.

The invention discloses a gene and its protein of phosphomannose isomerase, and its application. The purpose is that providing a gene and its protein of phoshomannose isomerase, and applying the gene and protein in selecting trans-genic plant. The gene has one of nucleotide sequences as follow: 1) the SEQ ID NO: 1 DNA sequence in sequence table; 2) coding the DNA sequence of SEQ ID NO: 2 in the sequence table; 3) the nucleotide sequence that can hybrid with restrict DNA sequence of SEQ ID NO: 1 in the sequence table at high strict condition. Mannose has no harm for human and animal, degradation-liable in environment and selected marker system has the advantages of product yield safety, the selecting regent price is cheap, the selecting procedure is simplicity and the selected marker system don't impact the metabolism balance of transformation plant, the trans-genic plant grow vigorous, significance effect selection. The selected marker system has potential and extensive application outlook in trans-genic plant study and its market process; it may be the leading selected marker system in plant transformation.

The invention provides a method for introducing an exogenous gene into japonica rice with close glume pollination by using PMI (phosphomannose isomerase, phosphomannose isomerase) screening marker. Specifically, the present invention is mediated by the Agrobacterium strain EHA105 containing the pCAMBIA1381-PMI vector, and using mannose as a screening agent, the exogenous gene is introduced into the japonica rice variety 8m30 pollinated by close glumes. Detection and identification by PCR indicated that the exogenous gene had been introduced into 8m30. This method successfully realizes the genetic transformation of japonica rice varieties pollinated by closed glumes, and does not require the use of antibiotics or herbicides for screening. While speeding up the process of trait improvement, it avoids gene drift caused by pollen spreading, and improves the degree of environmental friendliness.

The invention relates to a fucosyllactose-producing recombinant expression plasmid carrier, a metabolic engineering bacterium and a production method, and belongs to the fields of metabolic engineering and food fermentation technology; the invention synthesizes fucose de novo by expressing it in Corynebacterium glutamicum Encodes GDP‑mannose‑6‑dehydrogenase, GDP‑fucose synthase, lactose permease, and α‑1,2‑fucosyltransferase or α‑1,3‑ulosyltransferase required for the lactose-based pathway The gene of alcosyltransferase is used to construct recombinant Corynebacterium glutamicum to realize the synthesis of 2'-fucosyllactose or 3-fucosyllactose; Sugar isomerase, phosphomannose mutase and mannose-1-phosphate guanine base transferase genes can obtain high yield of fucosyllactose; the engineering bacteria obtained by the method of the present invention can utilize glucose or glycerol to synthesize fucose Lactose-based, has the advantages of fast growth and high safety, and has obvious potential for industrial production.

The invention provides a method of effectively introducing exogenous genes into rice cells by utilizing PMI (phosphomannose isomerase) selective marker, a transformation system, as well as a preparation method of genetically modified rice by utilizing the method.