35 results about "Phosphomannose Isomerase" patented technology

The invention provides phosphomannose isomerase from chlorella variabilis. The phosphomannose isomerase from the chlorella variabilis is named as ChloPMI. The invention further provides a prokaryotic expression vector containing the ChloPMI, and the prokaryotic expression vector can be used for identifying the proteometabolism mannose activity of the ChloPMI. The invention further provides an expression cassette containing the ChloPMI, a plant expression vector, application of the expression cassette and the application of the plant expression vector on the aspect of plant genetic transformation. The plant expression vector constructed by utilizing the ChloPMI uses mannose as a screening reagent and successfully achieves conversion of rice cells. The phosphomannose isomerase is successfully separated and cloned from the chlorella variabilis, due to the facts that the chlorella variabilis derives from the chlorella variabilis, is environmentally friendly and has no potential risks to the human body, application and popularization of transgenic products are facilitated, and doubts about transgenosis are cleared.

The invention relates to a fucosyllactose-producing recombinant expression plasmid carrier, a metabolic engineering bacterium and a production method, and belongs to the fields of metabolic engineering and food fermentation technology; the invention synthesizes fucose de novo by expressing it in Corynebacterium glutamicum Encodes GDP‑mannose‑6‑dehydrogenase, GDP‑fucose synthase, lactose permease, and α‑1,2‑fucosyltransferase or α‑1,3‑ulosyltransferase required for the lactose-based pathway The gene of alcosyltransferase is used to construct recombinant Corynebacterium glutamicum to realize the synthesis of 2'-fucosyllactose or 3-fucosyllactose; Sugar isomerase, phosphomannose mutase and mannose-1-phosphate guanine base transferase genes can obtain high yield of fucosyllactose; the engineering bacteria obtained by the method of the present invention can utilize glucose or glycerol to synthesize fucose Lactose-based, has the advantages of fast growth and high safety, and has obvious potential for industrial production.