Preparation method of hexose-6-phosphoric acid composition and application of hexose-6-phosphoric acid composition in cosmetics

A technology of composition and phosphoric acid, which is applied in the direction of cosmetics, cosmetic preparations, medical preparations containing active ingredients, etc., can solve the problems of different timeliness, etc., and achieve the effect of reducing process difficulty, saving production cost, and reducing process routes

Active Publication Date: 2022-06-21
北京焉支山科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]However, due to the differences in the pathways involved in metabolism, the timeliness of different hexose phosphates as whitening and anti-wrinkle cosmetics is not the same

Method used

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  • Preparation method of hexose-6-phosphoric acid composition and application of hexose-6-phosphoric acid composition in cosmetics
  • Preparation method of hexose-6-phosphoric acid composition and application of hexose-6-phosphoric acid composition in cosmetics
  • Preparation method of hexose-6-phosphoric acid composition and application of hexose-6-phosphoric acid composition in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1: Genetically engineered bacteria I prepare hexokinase crude enzyme liquid by fermentation

[0067] (1) Bacterial fermentation:

[0068] The genetically engineered bacteria I capable of heterologously expressing polyphosphate-dependent mannokinase were inoculated into 50 mL of LB liquid medium (50 mg / L Kana), fermented for 8 h, at a temperature of 37 °C, and at a stirring speed of 180 rpm; The fermentation product was inoculated into 2L of LB liquid medium (50mg / L Kana) according to 1‰, and the air flow was controlled to be 4Nm under the conditions of temperature 30℃ and pH 7 3 / h; after the dissolved oxygen in the fermentation system is stabilized at 10%, an inducer IPTG is added, and the above fermentation conditions are maintained for 12 hours to obtain a fermentation broth.

[0069] (2) Preparation of crude enzyme solution:

[0070] The fermentation broth was centrifuged at 4°C and a rotating speed of 8000rpm to collect the cells, and the cells were re...

Embodiment 2

[0076] Example 2: Preparation of phosphate mannose isomerase crude enzyme liquid by fermentation of genetically engineered bacteria II

[0077] (1) Bacterial fermentation:

[0078] The genetically engineered bacteria II capable of heterologously expressing phosphomannose isomerase was inoculated into 50 mL of LB liquid medium (50 mg / L Kana), and fermented for 8 h at a temperature of 37°C and a stirring speed of 180 rpm; 0.5‰ was inoculated into 2L of LB liquid medium (50mg / L Kana), and the air flow was controlled to be 4Nm under the conditions of temperature of 30℃ and pH of 7 3 / h; after the dissolved oxygen in the fermentation system is stabilized at 10%, an inducer IPTG is added, and the above fermentation conditions are maintained for 12 hours to obtain a fermentation broth.

[0079] (2) Preparation of crude enzyme solution:

[0080] Carry out according to the method described in Example 1.

[0081] (3) Enzyme activity assay:

[0082] Accurately weigh 1mL of the crude ...

Embodiment 3

[0086] Embodiment three: the joint fermentation preparation of mixed enzyme crude enzyme liquid

[0087] (1) Bacterial fermentation:

[0088]The genetically engineered bacteria I capable of heterologously expressing polyphosphate-dependent mannokinase was inoculated into 50 mL of LB liquid culture medium (50 mg / L Kana); at the same time, the heterologous expression of mannose phosphate isomer The genetically engineered bacteria II of the enzyme was inoculated into 50 mL of LB liquid medium (50 mg / L Kana); the fermentation conditions of both were 37 ° C, 8 h, and 180 rpm; and then the fermentation products of the genetically engineered bacteria I were 1‰, gene The fermentation product of engineering bacteria II was inoculated into 2L of LB liquid medium (50mg / L Kana) according to 0.5‰, and the air flow was controlled to be 4Nm under the conditions of temperature of 30℃ and pH of 7 3 / h; after the dissolved oxygen in the fermentation system is stabilized at 10%, an inducer IPTG...

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Abstract

The embodiment of the invention relates to a preparation method of a hexose-6-phosphoric acid composition and application of the hexose-6-phosphoric acid composition in cosmetics. The preparation method comprises the following steps: S1, inoculating a genetically engineered bacterium I for expressing mannose kinase and a genetically engineered bacterium II for expressing phosphomannose isomerase into an LB liquid culture medium, and carrying out aerobic fermentation to obtain a liquid combined fermentation product; s2, separating the liquid-state combined fermentation product to obtain a compound enzyme crude enzyme solution; s3, performing enzyme catalytic reaction on the crude enzyme liquid of the compound enzyme to obtain a mixed product system; and S4, carrying out separation treatment on the mixed product system to obtain the hexose-6-phosphoric acid composition. According to the method, the hexose-6-phosphoric acid does not need to be refined and dried, so that the production and application cost is reduced; cell experiments, skin tests and other means prove that the hexose-6-phosphoric acid composition has good effects of improving skin tone, repairing aged skin, relieving skin wrinkles and the like.

Description

technical field [0001] The invention relates to the technical field of biological composition preparation, in particular to a preparation method of a hexose-6-phosphate composition and its application in cosmetics. Background technique [0002] As an important organ to protect the human body, the skin has a metabolic mechanism based on material and energy metabolism. Among them, the cells of the stratum corneum of the skin migrate from the basal layer and contain substantially no cytoplasm, and eventually die and slough off on the skin surface. The surface cells of normal young skin are continuously shed under the action of metabolism, so that the skin can be kept smooth and vibrant. However, when material and energy metabolism in basal cells is disrupted or cannot be performed efficiently, then dead cells accumulate in the stratum corneum and lead to wrinkled, dull, and scarred skin. For this reason, cosmetics that strengthen and enhance the skin's metabolic process and k...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12P19/24A61K8/60A61Q19/02A61Q19/08
CPCC12P19/02C12P19/24A61K8/60A61Q19/02A61Q19/08A61K2800/85A61K2800/805
Inventor 殷昊
Owner 北京焉支山科技有限公司
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