6 results about "LacZ Genes" patented technology

The invention relates to a polynucleotide comprising a lacZ gene (lacZFS) encoding a β-galactosidase characterized by a particular profile with respect to its lactose hydrolysis efficiency. The invention also relates to a strain of Streptococcus thermophilus comprising a lacZFS allele and a bacterial composition thereof, and its use for obtaining fermented milk that does not undergo subsequent acidification.

This invention relates to the field of bioengineering, and more particularly to genomes, expression cassettes, expression vectors, recombinant strains, and their applications. The invention provides a genome including the NGF gene, the malF gene, and the lacZ gene. This invention provides a reliable and efficient system and method for expressing and screening disulfide bond-rich proteins, which not only has broad application prospects in the expression of disulfide bond-rich proteins, but also provides new ideas and methods for research and applications in related fields.

The invention discloses a biosynthesis method of alpha-lipoic acid, an engineering strain and a preparation method of the engineering strain. The construction of the engineering strain comprises the following steps: transforming lipocaprylamide enzyme Lpa of enterococcus faecalis, constructing the lipocaprylamide enzyme Lpa to an arabinose promoter or a Cold temperature-sensitive promoter, and then replacing the lipocaprylamide enzyme Lpa to a LacZ gene and an lpxM gene of escherichia coli through RED recombination; or the genes of LipD, lplA, lipA and lpA which are subjected to gene code modification are expressed in the saccharomyces cerevisiae through a pESC vector. The engineering strain is cultured to biologically synthesize alpha-lipoic acid, so that the fermentation cost is further reduced, cyclic synthesis is realized, and the yield is greatly increased.

The invention relates to a polynucleotide comprising a lacZ gene (lacZFS) encoding a β-galactosidase characterized by a particular profile regarding its efficiency of hydrolysis of lactose. The invention is also directed to a Streptococcus thermophilus strain comprising a lacZFS allele and bacterial composition thereof, and their use to obtain fermented milk not undergoing post-acidification.

This invention provides genetically modified bacteria that produce L-valine, as well as a method for constructing and using such bacteria. By knocking out the yjiT gene of E. coli to express brnF and brnE, knocking out the yjiV gene to express ilvE and ilvD, knocking out the trpR gene to express the ilvH mutant protein, knocking out the lacI and lacZ genes to express DNA polymerase, and / or knocking out the ycgH gene to express ilvC, the resulting genetically modified bacteria can increase L-valine production. These genetically modified bacteria can be used for L-valine production and have promising application prospects.

This invention relates to the field of biotechnology, specifically disclosing a cell-free protein expression system (CFS), its applications, and a kit. The CFS comprises: 35-50 vol% *E. coli* cell extract, 40-50 vol% lysis buffer, 8-12 vol% gene template, and 2-15 vol% nuclease-free water; wherein the *E. coli* is a recombinant bacterium obtained by modifying a recipient *E. coli*, the modification including: (1) knocking out the LacZ gene in the recipient *E. coli*; and (2) adding the LyseR gene to the recipient *E. coli*. The CFS of this invention features high expression activity and gene stability, making it suitable for expressing high molecular weight proteins and for in vitro diagnostic applications based on cell-free sensors.