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Method for producing erythritol by using broken rice

A technology of erythritol and broken rice, which is applied in the field of enzyme engineering and microbial fermentation, can solve the problems of complex erythritol separation process, long production cycle, and many by-products, and avoid the use of a large amount of elution solvents, The effect of reducing production costs and reducing separation costs

Inactive Publication Date: 2011-02-23
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on erythritol in our country is relatively late, and the currently used strains include Torula, Torulopsis, and Candida, etc., but the yield is mostly low, there are many by-products, and the production technology is relatively backward. The separation process of erythritol in liquid is complicated, and the purity of erythritol crystals is low, resulting in high production cost of erythritol
In terms of carbon source utilization, cornstarch fermentation is mainly used to produce erythritol in industrial production, which has the disadvantages of high cost, low conversion rate, long production cycle, darker color of fermentation liquid, and complicated separation.
Japanese scholars have studied the production method of using glycerol and alkanes as fermentation carbon sources to ferment and produce erythritol, but it has not yet been industrialized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Production of broken rice sugar liquid

[0031] Wash the broken rice by-product of indica rice, pick out the impurities in the broken rice, soak in water at 40℃ for 2h, then refine with a refiner to a fineness of 40 mesh, add water to adjust the thick slurry to a concentration of 20°Bé. Get broken rice and rice milk;

[0032] Add liquid high-temperature resistant α-amylase into the broken rice milk, and add 160 mL of high-temperature resistant α-amylase per ton of broken rice. Use a high-temperature jet liquefier for liquefaction, the first jet temperature is 105°C, and the pressure is maintained for 15 minutes. The second injection temperature is 130℃, and the pressure is maintained for 2min. After cooling down to 100℃, pump into the laminar flow tank, add the same high-temperature α-amylase reaction again, add 240mL high-temperature α-amylase per ton of broken rice, reaction time 90min, reaction temperature 100℃, when the solid content is At 20%, the end of the re...

Embodiment 2

[0046] (1) Production of broken rice sugar liquid

[0047] Wash the broken rice by-product of the japonica rice, pick out the impurities in the broken rice, soak in water at 40℃ for 6 hours, and then refine with a refiner to a fineness of 40 mesh. Add water to adjust the thick slurry to a concentration of 18°Bé. Get broken rice and rice milk;

[0048] Add liquid high-temperature resistant α-amylase into the broken rice milk, and add 160 mL of high-temperature resistant α-amylase per ton of broken rice. Use a high-temperature jet liquefier for liquefaction, the first jet temperature is 105°C, and the pressure is maintained for 15 minutes. The second injection temperature is 130℃, and the pressure is maintained for 2min. After cooling down to 100℃, pump into the laminar flow tank, add the same high temperature resistant α-amylase reaction again, add 240mL high temperature resistant α-amylase per ton of broken rice, reaction time 100min, reaction temperature 95℃, when the solid co...

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Abstract

The invention relates to a method for producing erythritol by using broken rice. In the method, moniliellaacetoabutans (ATCC18455) is used as a production strain and broken rice sugar liquid is used as a fermentation substrate for preparing the high-purity erythritol; and the method comprises three steps, namely preparation of the broken rice sugar liquid, preparation of erythritol fermentation liquid and refining of an erythritol product. The erythritol has high transformation rate, short fermentation period and stable fermentation performance, and has no byproducts such as glycerol and the like; the method reduces the production cost, and has the advantages of low operating cost, small equipment space, high product yield, few impurities and simple process; and the purity of the final product reaches over 98 percent.

Description

Technical field [0001] The present invention belongs to the technical field of enzyme engineering and microbial fermentation, and specifically relates to the use of Acetomyces esculentus ( Moniliella acetoabutans ATCC 18455) as the starting strain, using the broken rice sugar solution after enzymatic hydrolysis as the fermentation substrate to produce erythritol by fermentation. Background technique [0002] Erythritol is a four-carbon sugar alcohol with a sweetness of about 70% of sucrose. Its chemical name is 1,2,3,4-butanetriol and its molecular formula is C. 4 H 10 O 4 . Erythritol is widely distributed in nature and exists in mushrooms, alcohol, soy sauce, cheese, tissue fluids and body fluids of humans and animals. Because of the unique metabolism of erythritol in the human body, erythritol has many excellent physiological functions, such as zero calorie value, high tolerance, low side effects, suitable for diabetics, non-cariogenic properties , Scavenging free radical fun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C12P19/14C12R1/645
Inventor 王泽南刘鹏李莹张秋子吴红引苏娅
Owner HEFEI UNIV OF TECH
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