The invention discloses needle mushroom biscuits and a preparation method thereof. The needle mushroom biscuits are prepared from the following raw materials in parts by weight: 40-60 parts of needle mushrooms, 150-180 parts of flour, 18-24 parts of olive juice, 20-30 parts of pawpaws, 10-20 parts of fructo-oligose, 6-8 parts of wheat sprouts, 8-10 parts of durian seed kernels, 4-6 parts of aonori powder, 10-20 parts of green peppers, 10-20 parts of taros, 1-2 parts of momordica grosvenori leaves, 1-2 parts of haw flowers, 2-3 parts of pineapple leaves, 1-2 parts of flores aurantii, 3-5 parts of natto powder, 3-6 parts of xanthan gum, 20-40 parts of rose wine, 1-2 parts of baking soda, an appropriate amount of linseed oil, and 8-12 parts of a nutrition additive. The needle mushroom biscuits prepared by the method disclosed by the invention are fragrant, crisp and soft, and adopt the rose wine for soaking the needle mushrooms, so that the nutrient ingredients of the needle mushrooms are reserved furthest, the flavor and the taste of the biscuits are improved, the immunity function of human bodies is improved, and the nutrition is rich and balanced; varied natural plants are added for replacing tea ingredients, so that the effects of aroma enhancement and seasoning are realized, the digestion is promoted and the health of the human bodies is facilitated.