7results about "Maltose production" patented technology

This invention discloses a mutant of raw starch hydrolase with improved stability and its applications. Using α-amylase AmyZ2 as the starting enzyme, and through computer-aided design to clarify the mutation site, a mutant was heterologously expressed in Bacillus subtilis, resulting in an α-amylase with improved stability. When using raw corn starch as a substrate, the mutant exhibits 1.57 times the specific enzyme activity and 2 times the stability of the starting enzyme. While maintaining the specific enzyme activity, the mutant enzyme shows a significant improvement in thermostability. This mutant has potential application value in the production of starch sugars using raw corn starch as a substrate.

The utility model relates to the technical field of evaporation and concentration, discloses a maltose evaporation and concentration device, and solves the problems that when maltose is evaporated through a heating pipe, the maltose close to a container is gradually reduced after being evaporated, and in the reduction process, the inner wall of the container is directly contacted with the maltose, so that the maltose cannot be evaporated. The problems that the evaporation efficiency in a container is reduced due to the fact that maltose adheres to the inner wall of the section, where maltose drops, of the container, coking is caused, and meanwhile due to the fact that the maltose is high in adhesion, most maltose adheres to the inner wall of the container in the discharging process, and cleaning is difficult are solved. An exhaust pipeline is installed on the outer side of the concentration tank, a connecting device is installed at the upper end of the concentration tank, the concentration tank comprises a heat preservation outer shell, a bottom plate is fixedly installed at the lower end of the heat preservation outer shell, a heating pipe is installed on the inner side of the heat preservation outer shell, a heat preservation inner shell is installed on the inner side of the heating pipe, and a stirring assembly is installed in the heat preservation inner shell.

The present invention relates to a method for producing disaccharides using beta-glucosidase and its cofactors, and a composition for inducing enzyme production in a Trichoderma strain, which contains the produced disaccharides. Specifically, the method comprises inducing beta-glucosidase and a divalent metal ion, manganese ion (Mn ion), as a cofactor, on a glucose substrate. 2+ ), magnesium ion (Mg 2+ ), zinc ion (Zn 2+ ) or copper ions (Cu 2+ ), the disaccharide production rate can be increased and disaccharides can be obtained at a high concentration. In addition, since the composition with a high content of disaccharides produced by the production method of the present invention has an excellent effect of inducing enzyme production in Trichoderma strains, it is possible to improve the enzyme productivity of Trichoderma by applying a fermentation process that utilizes the produced disaccharides at a high concentration.

The present disclosure is directed to the generation and use of a synthetic tetrasaccharide designed based on a portion of a mycobacterial polar glycolipid as a vaccine antigen to limit or prevent tuberculosis infections.

The invention discloses a method for relieving microbiological contamination in the saccharification process of malt syrup production, and belongs to the technical field of starch sugar production. The method comprises the following steps: adjusting the pH value of starch milk to 5.1-5.4, adding acid-resistant alpha-amylase for spray liquefaction, and then performing secondary spray enzyme deactivation to obtain liquefied liquid; carrying out vacuum flash evaporation on the liquefied liquid, cooling to 60.5-64.0 DEG C by using a plate heat exchanger, adding the liquefied liquid and saccharifying enzyme into a saccharifying tank, and saccharifying to obtain saccharified liquid; and finally, carrying out decoloration, filtration, ion exchange treatment and vacuum concentration on the saccharified liquid to obtain the malt syrup. According to the method, by selecting the acid-resistant amylase and adjusting the initial pH and the saccharification temperature, bacterial contamination in the saccharification process of maltose production is remarkably relieved, so that the problem of bacterial contamination in the saccharification process is solved, unstable saccharification quality caused by bacterial contamination in the saccharification process is avoided, metabolites generated by bacterial contamination are reduced, the refining burden is relieved, and the saccharification quality is improved.

The invention discloses a production method and device of malt syrup, and relates to the field of food production. In the production process of the malt syrup, a secondary injection technology is reasonably arranged, so that the DE value of the obtained finished product is kept in a range of 38-46, meanwhile, the iodine value of the finished product is reduced, and the filtering speed of the material is increased; the plate-and-frame filter press and the rotary drum filter are used and cooperate to improve the feeding speed and the discharging quality; and the falling film evaporator MVR is used in the evaporation process, so that the production efficiency is improved.

The utility model relates to evaporation equipment technical field discloses a kind of multiple-effect evaporation device, including multiple evaporators connected in series in turn, the steam outlet of previous evaporator is connected with the steam inlet of following evaporator, first group multiple-effect evaporator is for several in front of multiple evaporators, second group multiple-effect evaporator is for several behind;The outlet of feed pipeline is connected with the material inlet of the evaporator of the first evaporator of second group multiple-effect evaporator most front part, the material outlet of previous evaporator in second group multiple-effect evaporator is connected with the material inlet of following evaporator, the material outlet of last evaporator in second group multiple-effect evaporator is connected with the material inlet of last evaporator of first group multiple-effect evaporator, the material outlet of following evaporator in first group multiple-effect evaporator is connected with the material inlet of previous evaporator.A kind of maltose preparation equipment, including the multiple-effect evaporation device of any preceding. The present application has the advantages of high thermal efficiency, high evaporation efficiency and improving product quality.