45 results about "Heparinoids" patented technology

The present invention provides composition and methods for the synthesis of low molecular weight heparins and heparinoids. The present invention also provides compositions having substantially homogenous populations of desired heparin molecules, or molecules useful in the synthesis of heparin oligosaccharides.

Preparation of synthetic monosaccharides, disaccharides, trisaccharides, tetrasaccharides and pentasaccharides for use in the preparation of synthetic heparinoids.

The invention discloses a synthesis method of a 6-O-carboxymethyl chitosan sulfation product. The method comprises the following steps: (1) chitin alkaline treatment of chitin; (2) carboxymethylation of C6-O site of chitin; (3) deacetylation reaction of 6-O-carboxymethyl chitin; and (4) sulfation of 6-O-carboxymethyl chitosan. The synthesis method disclosed by the invention is a bran-new method for selectively replacing and controlling the replacement rate by using chitin. The synthesis product of the method provides N-site -SO3H with main anticoagulant activity and introduces -COOH, so that a lot of -COOH and SO3H which have negative electricity in the molecular structure are regularly distributed, and an anticoagulant effect of heparinoid is generated by the synergistic effect. High molecular polysaccharide is a heparinoid drug which is selectively modified by chitin via a safe reagent, so that reagent pollution of bulk drugs is reduced, the virus contamination risk of heparin biological extraction is avoided, the safety performance of the drug in the clinical experiment is more excellent in theory as compared with heparin sodium, so the 6-O-carboxymethyl chitosan sulfation product provided by the invention is expected to serve as a cheap direct thrombin inhibitor to replace heparin sodium anti coagulation drugs.

Dermatan sulfates and/or O-desulfated heparins useful in treating and preventing heparinoid-induced autoimmune responses, in particular heparin-induced thrombocytopenia (HIT) and its associated disease states. The dermatan sulfates comprise repeating disulfated and/or trisulfated disaccharide units of L-iduronic acid and N-acetyl-D-galactosamine. The O-desulfated heparins comprise heparin molecules selectively O-desulfated at the 2-O and/or 3-O positions of the uronic acid and glucosamine saccharide residues. Particularly effective dermatan sulfate HIT antagonists have a mean molecular weight of from about 2000 to about 10,000 Daltons.

The invention discloses a heparinoid gel microsphere. The heparinoid gel microsphere is prepared through coagulating bath reaction between a polymer solution and a heparinoid reaction fluid; and the heparinoid gel microsphere has a functional group similar to anticoagulant heparin. The heparinoid gel microsphere disclosed by the invention has the advantages that the anticoagulation property is excellent, the dimension is stable, and breakage cannot be caused by a reason of swelling or a pressure in the blood treatment process. The invention further provides a preparation method of the heparinoid gel microsphere; and the preparation method of the heparinoid gel microsphere is simple and suitable for industrialization. The invention further discloses a purpose of the heparinoid gel microsphere and can be used for the field of blood purification.

The invention relates to a heparinoid-modified polyvinyl alcohol hydrogel thin nano-compound hematodialysis film and a preparation method thereof. A heparinoid hydrogel skin layer is arranged as an outer layer of a thin nano-compound hematodialysis film and a porous supporting layer with nano-pores communicated with each other is arranged as an inner layer of the thin nano-compound hematodialysis film. The preparation method comprises the following steps: performing electrostatic spinning on a PAN solution, thereby acquiring a nanometer fiber as the supporting layer of a compound film; adding sodium-hydrogen into the PVA solution, and then adding 1,3-propane sultone, reacting for 8-24h at 40-80 DEG C, filtering and drying, thereby acquiring s-PVA; mixing s-PVA with PVA, adding a solvent, adjusting pH, adding a cross-linking agent, coating on the supporting layer after cross-linking, and sealing at room temperature, thereby acquiring the heparinoid-modified polyvinyl alcohol hydrogel thin nano-compound hematodialysis film. The preparation method provided by the invention is simple in reaction process and is easily performed. The prepared thin nano-compound hematodialysis film has the characteristics of permanent hydrophily, low protein adsorbability and excellent biocompatibility.

The present invention relates to inhibition of heparinoids anti-coagulation activity by a non-active form of a eukaryotic endoglycosidase or any fragment or peptide thereof comprising at least one heparin-binding domain. More particularly, the invention provides compositions and methods for the inhibition of heparinoids anti-coagulation activity and for the treatment of coagulation related pathologic clinical conditions, using a non-active form of mammalian heparanase or peptides thereof comprising at least one heparin-binding domain.

The invention discloses a preparation method of heparinoid. The preparation method comprises the following steps : adopting chondroitin sulfate as a raw material, and treating to obtain heparinoid after sulfonation reaction. In the sulfonation reaction, the adopted solvent is formamide and the adopted sulfonating agent is free sulfur trioxide, fuming sulfuric acid or pyridine sulfur trioxide. The preparation method of heparinoid, disclosed by the invention, has the advantages that the synthesis process is simple, the production and the operation are easily controlled, no special requirement exists for production equipment, the molecular weight range of a product is stable, the physicochemical indexes of the product are controlled in the quality standard range, and simultaneously the product is high in yield and the method is suitable for large-scale industrial production.

The invention relates to methods of treating and preventing radiation damage from whole-body exposure. According to the methods of the invention, subjects are treated therapeutically and/or prophylactically with low-anticoagulant heparinoids. The invention also relates to methods of extending the life of subjects exposed to whole-body radiation.

The disclosure provides in vitro and in vivo methods for identifying Heparins and Heparinoids that modulate the activity of selectins . The disclosure also provides Heparins and Heparinoids that modulate the activity of selectins . The identification and isolation of these heparin formulations has the potential to mediate a wide variety of pathologies mediated by P- and/or L-selectin, including hematogenous metastasis, diseases associated with inflammation (e.g., asthma, arthritis, allergic dermatitis), ischemia-reperfusion injury, or other pathologies such as sickle cell anemia. Selectin inhibition can be achieved at plasma concentrations lower than those that cause excessive anticoagulation or unwanted bleeding in a human subject.

The invention relates to a process for extracting heparinoid or protein from heparin by-products. The method comprises the following steps: (1) adding hot water into the heparin by-products for dissolving, and then adding sodium chloride until the heparin by-products are completely dissolved; (2) after the heparin by-products are completely dissolved, adding an enzyme reagent A, adjusting the pH to 8-10, heating and then cooling to the room temperature, and centrifuging to obtain first centrifugate; (3) adding a precipitant B into the first centrifugate, stirring, and centrifuging to obtain second centrifuged wet solid; (4) adding hot water into the second centrifuged wet solid for dissolving, and then adding the sodium chloride until the solid is completely dissolved; (5) after the secondcentrifuged wet solid is completely dissolved, adding ethanol for precipitating, and collecting precipitate I; (6) adding water for dissolving the precipitate I, and carrying out ultrafiltration to obtain ultrafiltrate; (7) adding the sodium chloride into the ultrafiltrate for dissolving, then adding ethanol, and collecting precipitate II; (8) drying the precipitate II to obtain the heparinoid orthe protein. The process has the advantages that the process can improve the recovery rate and titer of the heparinoid or the protein.

The invention belongs to the technical field of medical devices, and discloses a covered implantable medical device and a preparation method thereof. The implantable medical device comprises a skeleton material, the surface of the skeleton material is sequentially covered with a coating and a thermoplastic polymer film, the surface of the thermoplastic polymer material film is grafted with an anticoagulant compound; the coating is formed by condensation polymerization of diamine A and dibasic acid B in the form of ABAB head-to-tail bonding on the skeleton surface through amidation reaction; the anticoagulant compound has carboxyl or sulfonic acid groups, specifically one or more of VA64, low molecular weight heparin, heparinoid copolymer and diosgenin. Bonding strength between the polymermaterial and the skeleton material is improved by arranging the coating on the surface of the skeleton material, anticoagulation modification is performed on the covered skeleton material, and the problem about blood compatibility of the implantable device is solved.

The invention belongs to the technical field of biological fibers and functional materials and specifically relates to a low density lipoprotein adsorption microsphere and a preparation method and anadsorption material. The low density lipoprotein adsorption microsphere comprises a carrier; a hydrophobic functional group and heparinoid are coupled to the carrier; the mass ratio of the carrier, the heparinoid and the hydrophobic functional group is 1:(0.1-0.5):(0.1-0.5); the hydrophobic functional group consists of Delta-tocopherol and Alpha-tocopherol, wherein the content of the Delta-tocopherol does not exceed 5-8wt%. The low density lipoprotein adsorption microsphere provided by the invention has the advantages that the adsorption selectivity for LDL in whole blood is high, the adsorption rate of the LDL can reach up to 95% and above; more importantly, the damage to erythrocyte is low; the erythrocyte decrement rate is only 3.21%; and meanwhile, the filtering speed is high, the costis low, and the low density lipoprotein adsorption microsphere is suitable for large-scale promotion and application.