The invention relates to a process for extracting
heparinoid or
protein from
heparin by-products. The method comprises the following steps: (1) adding hot water into the
heparin by-products for dissolving, and then adding
sodium chloride until the
heparin by-products are completely dissolved; (2) after the heparin by-products are completely dissolved, adding an
enzyme reagent A, adjusting the pH to 8-10, heating and then cooling to the
room temperature, and centrifuging to obtain first centrifugate; (3) adding a precipitant B into the first centrifugate, stirring, and centrifuging to obtain second centrifuged wet
solid; (4) adding hot water into the second centrifuged wet
solid for dissolving, and then adding the
sodium chloride until the
solid is completely dissolved; (5) after the secondcentrifuged wet solid is completely dissolved, adding
ethanol for precipitating, and collecting precipitate I; (6) adding water for dissolving the precipitate I, and carrying out
ultrafiltration to obtain ultrafiltrate; (7) adding the
sodium chloride into the ultrafiltrate for dissolving, then adding
ethanol, and collecting precipitate II; (8)
drying the precipitate II to obtain the
heparinoid orthe
protein. The process has the advantages that the process can improve the
recovery rate and
titer of the
heparinoid or the
protein.