Process for extracting heparinoid or protein from heparin by-products
A technology of heparin by-product and heparin is applied in the process field of extracting heparin or protein from heparin by-product, which can solve the problems of low titer of heparin and low recovery rate of heparin, and achieve the effect of improving the yield
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment
[0024] The process for extracting heparinoids from heparin by-products in this embodiment comprises the following steps:
[0025] (1) Dissolve 80kg of heparin by-products in 1000kg of hot water, then add 50kg of analytically pure sodium chloride with a mass percentage of 5% until completely dissolved;
[0026] (2) After 80 kg of heparin by-products are completely dissolved, add 3 kg of enzyme reagent, which is made of 14 parts of cysteine, 2 parts of trypsin and 0.5 part of sodium sulfite in parts by weight, and adjust the pH to 9; then heat to 55°C and keep it warm for 2 hours, then raise the temperature to 100°C, and finally cool down to room temperature with circulating water. After cooling down to room temperature, the first centrifugation removes solid impurities to obtain 1200kg of first centrifugate;
[0027] (3) Add 12kg of precipitant to the first centrifugal liquid, which is made of 1.5 parts by weight of 5-sulfosalicylic acid, 22.5 parts of acetone, 57.5 parts of me...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com