Separation method of heparan sulfate and dermatan sulfate in heparinoid

A technology of heparan sulfate and dermatan sulfate, which is applied in the field of medicine, can solve problems such as the inability to separate heparan sulfate and dermatan sulfate in heparan or precise quantitative control, and achieve the effect of easy amplification, good effect and avoiding loss

Active Publication Date: 2019-08-27
SUZHOU WISMED PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the content of chondroitin sulfate and dermatan sulfate can be precisely and quantitatively controlled in the above invention, the separation or precise quantitative control of heparan sulfate and dermatan sulfate in heparan cannot be realized.

Method used

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  • Separation method of heparan sulfate and dermatan sulfate in heparinoid

Examples

Experimental program
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Embodiment 1

[0021] This embodiment provides a method for separating heparan sulfate and dermatan sulfate in heparan, which comprises the following steps:

[0022] (a) 40 g of heparinoid (specific optical rotation +5°, anticoagulant factor Xa activity 15 IU / mg) was dissolved in 400 ml of deionized water to prepare a 10% heparinoid solution;

[0023] (b) Inject the heparinoid solution into a polyacrylate anion exchange column with a column volume of 4 L for adsorption;

[0024] (c) Use 0~1mol / L sodium chloride solution for gradient elution (that is, linear gradient elution), the elution time is 100 minutes, and collect the eluent of each component; track with an ultraviolet detector (210nm ) to get as figure 1 The spectrogram shown;

[0025] (d) Concentrate the eluent of each component respectively (concentrate the eluent of each component to a sodium chloride concentration of 3~4wt%), add ethanol precipitation (so that each component elutes the ethanol in the mixed solution The concentr...

Embodiment 2

[0028] This example provides a method for separating heparan sulfate and dermatan sulfate in heparan, which is basically the same as the method in Example 1, except that: in step (a), a 5% heparan solution is prepared; finally 17 g of heparan sulfate and 17.5 g of dermatan sulfate were obtained.

Embodiment 3

[0030] This example provides a method for separating heparan sulfate and dermatan sulfate in heparan, which is basically the same as the method in Example 1, except that: in step (a), an 8% heparan solution is prepared; finally 17.2 g of heparan sulfate and 18.1 g of dermatan sulfate were obtained.

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Abstract

The invention relates to a separation method of heparan sulfate and dermatan sulfate in heparinoid. The separation method comprises the following steps of a, dissolving the heparinoid in water to prepare a heparinoid solution; b, adsorbing the heparinoid solution through anion resin; c, adopting a 0-1 mol / L sodium chloride solution for gradient elution to collect all component eluents; d, concentrating the component eluents separately and adding ethanol for precipitation to obtain the heparan sulfate and dermatan sulfate. In this way, the byproducts of heparinoid generated during production offine products of heparin from crude products of heparin can serve as raw materials, the loss of effective ingredients in the prior art is avoided, no impurities are introduced, the process is simple,the separation cost is low, the effect is excellent, and amplification is easy.

Description

technical field [0001] The invention belongs to the field of medicine and relates to a separation method, in particular to a separation method of heparan sulfate and dermatan sulfate in heparinoids. Background technique [0002] Heparin was first discovered in the liver and got its name. It is a mucopolysaccharide sulfate extracted from animals. It has anticoagulant and antithrombotic effects. It has been used to treat thrombosis since the 1930s. However, due to the different animal sources of heparin, there will be the possibility of species virus contamination, causing some problems when using heparin or low molecular weight heparin; at the same time, due to animal breeding and biochemical pollution during the extraction process of heparin, the heparin Applications are restricted to varying degrees. More than 40 years ago, when some European and American scientists headed by Lindahl and Choay were studying the anticoagulant and antithrombotic effects of heparin, they esta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10C08B37/08
CPCC08B37/0069C08B37/0075
Inventor 王芃
Owner SUZHOU WISMED PHARMA CO LTD
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