290results about "Gel preparation" patented technology

The preparation method comprises the following steps: mixing walnut oil and idesia oil according to a mass ratio of (85-90): (10-15), stirring and heating to 68-72 DEG C under the condition of a rotating speed of 55-70 rpm, adding a gel compounded by edible wax and polyglycerol fatty acid ester according to a mass ratio of (4-6): 1, continuing to stir until complete dissolution, adding an emulsifying agent, and uniformly stirring to obtain the beta '-crystal-form woody oil-based oleogel, so as to obtain the beta'-crystal-form woody oil-based oleogel, namely the beta '-crystal-form woody oil-based oleogel. The preparation method comprises the following steps: preparing a liquid grease gel solution, directly putting the liquid grease gel solution under the condition of-10 to-40 DEG C for quick freezing, then stirring for 3-6 minutes under the condition of the rotating speed of 150-250 rpm, and standing for 5-6 days under the environment of 20-25 DEG C to prepare the woody oil-based grease gel. The grease gel prepared by the method disclosed by the invention contains a stable beta'crystal form, is high in oil holding rate and proper in hardness, has relatively good oxidation stability, and can be applied to baking instead of animal butter or hydrogenated vegetable oil.

The invention discloses a preparation method of a salt-resistant double-layer photo-thermal aerogel evaporator. The preparation method comprises the following steps: S1, cutting and cleaning foamed nickel, and then carrying out vacuum drying; s2, preparing Cu2O / NF (nickel nitrate); s3, preparing ppy-Cu2O / NF (nickel fluoride); s4, preparing hydrogel; and S5, flatly placing a sample ppy-Cu2O / NF on the top surface of the hydrogel, continuing to stand for gelling, enabling the photo-thermal layer and the bottom layer hydrogel to be fully combined, pre-freezing, transferring into a freeze dryer, and freeze-drying to finally obtain the salt-tolerant double-layer photo-thermal aerogel evaporator, namely the lightweight porous aerogel material ppy-Cu2O / NF / FCP with the double-layer structure. The device has the advantages that the high-level evaporation rate and efficient energy conversion are achieved, and efficient photo-thermal conversion and heat management are achieved through the double-layer structure design.

The invention discloses a preparation method of DNA (Deoxyribonucleic Acid) hydrogel with programmable mechanical strength. By regulating and controlling the molar ratio of the catalytic initiating chains to the total amount of the DNA monomer building blocks, multiple hydrogels which are equivalent in mass concentration and have different mechanical strengths are constructed. According to the method, a strategy similar to catalytic assembly is adopted, the feeding molar ratio of a catalytic initiating chain to a DNA monomer building block is creatively adjusted, the proportion of cross-linking reactions at different levels is regulated and controlled, then the DNA hydrogel with different mechanical strengths is synthesized under the isothermal condition, and the storage modulus range is about 200-2000 Pa. In addition, the change of the mechanical strength is independent of the total concentration of the matrix (DNA) of the hydrogel, the DNA sequence, the stress relaxation rate and other properties. The method provides a novel convenient method for constructing DNA hydrogels with different mechanical strengths, and possibly provides a novel tool for researching the specific influence of mechanical properties of materials on cell / tissue behaviors.

The present invention relates to a colloidal composition comprising unmodified, discrete cellulose microparticles, such as a colloidal emulsion or colloidal gel, a method of preparing the colloidal composition and uses of the colloidal composition and cellulose particles The colloidal composition comprises a continuous phase and a dispersed phase, wherein the continuous phase is a liquid; wherein the dispersed phase comprises cellulose microparticles which are dispersed in the continuous phase of the colloidal composition, wherein the cellulose microparticles have a mean average particle length of from 0.1 to 20 µm a mean average particle width from 0.05 to 1.3 µm, and a maximum thickness not exceeding 0.2 µm determined by scanning electron microscopy; and an aspect ratio of length / width of 1 to 25, and wherein the colloidal composition has a flow index n of ≤ 1 determined by a rheometer at NTP (normal temperature (25°C) and pressure (1 atm)).

The invention provides polyacrylic acid-carboxylated chitosan interpenetrating network hydrophobic modified hydrogel as well as a preparation method and application thereof, and belongs to the technical field of wastewater treatment. The preparation method comprises the following steps: mixing an acrylic monomer, a hydrophilic acrylate monomer, an etherification type acrylamide monomer, an allyl quaternary ammonium salt type hydrophobic monomer, a crosslinking monomer and water, then mixing with a chitosan compound, and adjusting the pH value to obtain a reaction solution; and mixing the reaction liquid and a photoinitiator, then carrying out polymerization reaction, and sequentially pre-freezing and freeze-drying the obtained polymer hydrogel. Through multiple synergistic effects of photo-initiated rapid polymerization, amphiphilic hydrophobic modification, etherification auxiliary crosslinking and a chitosan compound interpenetrating network, the structural stability, complexing efficiency, industrial applicability and heavy metal adsorption performance are comprehensively improved; the adsorption rate and capacity of the hydrogel disclosed by the invention are obviously superior to those of the existing similar polyacrylic acid-based or chitosan-based hydrogel.

The invention relates to a moisture power generation device and a preparation method and application thereof, and belongs to the technical field of moisture power generation. The moisture power generation device comprises a flexible substrate, an upper electrode, a lower electrode and a power generation active material, the power generation active material is arranged between the upper electrode and the lower electrode; the power generation active material is of a core-sheath structure, a sheath layer on the edge is of a cross-linked hydrogel structure, and a core layer is a hydrogel precursor solution; the sheath layer limits the appearance of the hydrogel precursor solution in the core layer and is in contact with the atmosphere. The core-sheath structure of the power generation active material is prepared by an ultraviolet light induced polymerization / gelation one-step method. The moisture power generation device provided by the invention ensures stable and continuous electric energy output and remarkably improved moisture-electric energy conversion efficiency, adopts a miniaturized design, has the remarkable advantages of compact structure and convenience in integrated application, and is beneficial to large-scale production due to assembly convenience.

The invention relates to the field of functional gel composite material preparation and application thereof, in particular to an alpha-FeOOH gel composite material and a preparation method thereof.The preparation method includes the steps that 1, mixed solid powder is prepared and added into humic acid to be mixed to obtain gel, and the gel is added into a FeSO4 solution to be stirred and mixed; 2, a NaOH solution is dropwise added into the mixture, the mass ratio of FeSO4 to NaOH and the pH value of the NaOH solution are controlled, air is introduced for oxidation, and a supported alpha-FeOOH seed crystal suspension compound is obtained; 3, dispersing chitosan in a mixed aqueous solution of humic acid and acetic acid to obtain a mixed aqueous solution of chitosan and humic acid; 4, mixing the suspension compound with the mixed aqueous solution to obtain a cross-linked mixture solution; and 5, adding the FeSO4 solution again, introducing air to oxidize, and dropwise adding the NaOH solution to control the pH value of the cross-linked mixture solution, so that alpha-FeOOH is precipitated and grows on the seed crystal, and the alpha-FeOOH-gel slow-release composite material is obtained. The humic acid enables the degradable plastic to age, exposes functional groups, and is easier to mix.

This invention discloses an ultra-high toughness multifunctional self-assembled C-S-H gel material and its preparation method. The ultra-high toughness multifunctional self-assembled C-S-H gel material includes a stacked core-shell structure layer, which is composed of core-shell structure particles. The core-shell structure particles have hydrated calcium silicate nanoparticles as the core and polyacrylic acid-acrylamide copolymer as the shell. The obtained ultra-high toughness multifunctional self-assembled C-S-H gel material has high toughness, good tensile deformation properties, and temperature-sensitive effect.

The invention belongs to the technical field of scale inhibitors, and particularly relates to a slow-release scale inhibitor and a preparation method thereof.The preparation method comprises the steps that 3, 4, 5-allyl trihydroxybenzoate, itaconic acid, sodium hydrogen sulfite, methacryloyloxyethyl-n-hexadecyl-dimethyl ammonium bromide and deionized water are mixed, after water bath heating, an ammonium persulfate aqueous solution is slowly added, and the scale inhibitor is obtained after reaction; the preparation method comprises the following steps: dissolving agar in deionized water, adding polyethylene glycol modified sodium alginate and a scale inhibitor into the deionized water, stirring, dropwise adding into edible oil to obtain gel microspheres, adding the gel microspheres into a calcium chloride aqueous solution, slowly stirring, cross-linking, cleaning and drying to obtain the slow-release scale inhibitor. The hydrogel microspheres swell to slowly release the scale inhibitor, meanwhile, the released scale inhibitor is hydrolyzed in water, ester groups are slowly broken, polycarboxylic acid, 3, 4, 5-trihydroxybenzoic acid and hydroxyethyl-n-hexadecyl-dimethyl ammonium bromide are slowly released, and good scale inhibition and corrosion inhibition effects are achieved.

The invention belongs to the technical field of aviation fuel, and provides a method for producing sustainable aviation fuel by utilizing straws, which comprises the following steps: firstly, preparing synthesis gas from the straws; then carrying out a catalytic reaction on the synthesis gas and dimethyl ether through a primary molecular sieve catalyst, carrying out a catalytic reaction on the obtained catalytic product through a secondary metal catalyst, and separating to obtain ethanol; then preparing ethylene from the ethanol; and finally, preparing the aviation fuel from ethylene. The preparation method of the primary molecular sieve catalyst comprises the following steps: mixing a phosphoric acid solution with deionized water, adding a template agent according to a molar ratio of phosphoric acid to the template agent of 1: (0.5-0.55), and adding an aluminum source, a cerium source and silica sol while stirring to obtain gel; heating and crystallizing the gel; centrifugally washing the product to be neutral, drying and roasting; immersing the roasted product into a zinc nitrate solution, drying and calcining to obtain a zinc-loaded product; and performing dealumination treatment on the zinc-loaded product by using a citric acid solution, and roasting to obtain the zinc-loaded catalyst. According to the method, the ethanol yield in the aviation fuel production process can be remarkably improved.

The invention belongs to the technical field of photo-thermal material engineering, and particularly relates to a salt-resistant hydrogel evaporator utilizing solar evaporation for power generation and a preparation method and application thereof. The salt-tolerant hydrogel evaporator is prepared from the following raw materials: polyvinyl alcohol, hydrophilic conductive nanoparticles, bacterial nanocellulose and carbon nanotubes. Polyvinyl alcohol (PVA) is used as a framework, bacterial cellulose (BC) jointly forms a hydrogel structure with a hierarchical nanostructure, modified polyaniline conductive nanoparticles (CPs) are used for enhancing the electric signal transmission capacity of the hydrogel evaporator, and the salt-resistant hydrogel solar evaporator capable of realizing seawater desalination and electric power generation is prepared by adopting a simple and convenient method.

The invention discloses a method for wrapping stem cells by adopting programmable DNA (deoxyribonucleic acid) hydrogel and application of the method, under isothermal physiological conditions, three-dimensional DNA hydrogel is formed through a hybridization chain reaction of an initiation chain (I) and hairpin structure DNA to realize reversible wrapping and release of the stem cells. The hairpin structure DNA is formed by denaturation-renaturation treatment of at least two DNA monomer building blocks, and the mechanical rigidity of the hydrogel is realized by regulating and controlling the molar ratio of the initiation chain (I) to the total amount of the DNA monomer building blocks; the DNA hydrogel can reversibly wrap and release stem cells, and the wrapped stem cells can maintain activity and dryness and recover normal multiplication capacity after being released. According to the method disclosed by the invention, the activity and dryness of the stem cells are remarkably maintained, obvious immune activation is avoided, and the method provides a brand new tool for in-vitro culture and engineering application of the stem cells and has a wide application prospect.

The invention relates to core-shell hydrogel microsphere preparation equipment which comprises a microsphere preparation device and a microsphere collection device, the microsphere preparation device is provided with a nuclear phase inlet, a shell phase inlet, a plurality of nuclear phase distribution runners communicated with the nuclear phase inlet, and a plurality of shell phase distribution runners communicated with the shell phase inlet; the nuclear phase distribution flow channels and the shell phase distribution flow channels are converged into coaxial flow channels at the tail ends in a one-to-one correspondence manner; the microsphere collecting device comprises a microsphere collecting pool, the microsphere collecting pool is arranged below the coaxial flow channel, and the microsphere collecting pool is used for containing oil phase fluid; the nuclear phase fluid entering from the nuclear phase inlet and the shell phase fluid entering from the shell phase inlet form layered fluid at the coaxial flow channel, and the layered fluid is dropped into the microsphere collecting pool. According to the core-shell hydrogel microsphere preparation equipment provided by the invention, high-flux preparation of core-shell hydrogel microspheres can be realized.

The invention discloses a polyaluminum chloride-based flocculant as well as a preparation method and application thereof, belongs to the technical field of preparation of flocculants, and aims at solving the technical problem that the flocculation performance and the stability of a flocculant in the prior art need to be further improved. The surface charges of iron-based particles are adjusted through sodium citrate, so that the problem of particle agglomeration is effectively avoided, the coating uniformity of microgel is improved, and the specific surface area of the material is increased; abundant cation sites are introduced into the trimethylamine modified chitosan, so that the electrostatic adsorption capacity of the microgel on anion pollutants is remarkably enhanced, and meanwhile, the electrical property and the stability of a system are improved; the addition of sodium metasilicate further promotes the modified PAC to form a stable Al-O-Si bridging structure, the material is endowed with good three-dimensional skeleton strength and composite interface stability, the three components cooperate with each other and play key roles in particle dispersity, charge construction and skeleton stability, and finally, an efficient, stable and storable composite flocculation system is constructed.

The invention relates to the technical field of gas adsorption materials, in particular to a heteropoly acid modified GO-coated MMT composite material and a preparation method and application thereof. The preparation method comprises the following steps: ultrasonically dispersing graphite oxide in deionized water, stirring for 2-4 hours to obtain a graphite oxide solution, adding methylcyclopentadienyl manganese tricarbonyl into ethylene glycol, and stirring for 12-24 hours to obtain an MMT solution; pouring the graphite oxide solution into the MMT solution, reacting at 150-180 DEG C for 8-12 hours, washing, and freeze-drying to obtain GO-coated MMT composite hydrogel; and adding the GO-coated MMT composite hydrogel into deionized water, then adding heteropoly acid, stirring at 60-80 DEG C for 8-12 hours, and carrying out suction filtration and washing to obtain the heteropoly acid modified GO-coated MMT composite material. According to the heteropolyacid modified GO (at) MMT composite material disclosed by the invention, a complex with strong electrostatic adsorption force can be formed on the surface of GO (at) MMT by introducing heteropolyacid, and meanwhile, Bronsted acid sites and Lewis acid sites are increased, so that the adsorption performance is enhanced, and low-concentration ammonia gas adsorption is realized.

The continuous production device comprises a first tank body, a second tank body and a cooling box, first heating plates are fixedly installed at the two ends of the bottom of the first tank body, and a first electromagnetic valve is fixedly installed at the middle end of the bottom of the first tank body; and second heating plates are fixedly mounted at the two ends of the bottom of the second tank body. According to the invention, the extraction of the bamboo leaf extract and the preparation of the hydrogel are effectively linked, so that the production operation efficiency of the bamboo leaf hydrogel is effectively improved, and meanwhile, a group of cooling parts can be commonly used in the extraction process of the bamboo leaf extract and the preparation process of the hydrogel; and under the condition that bamboo leaf extract extraction and hydrogel preparation work do not interfere with each other, cooling positions can be correspondingly switched according to work requirements, and the use cost needed in the whole production and operation process is greatly reduced.

Titanium dioxide (TiO2) forms the basis of devices for applications including sensing devices, solar cells, photo-electrochromics, and photocatalysis. Such devices exploit different phases of TiO2 within such devices and accordingly it would be beneficial to have an amorphous TiO2 precursor which allows crystalline phase spatial patterning, for the crystallization of the amorphous TiO2 precursor to be triggered at low energies, and with the crystalline phase controllable at room-temperature without necessitating complex handling whilst providing TiO2 phases that are stable over a prolonged period of time. Accordingly, there are provided processes for providing a TiO2 precursor and controlling the conversion of the TiO2 precursor from amorphous-to-anatase, amorphous-to-rutile, amorphous-to-mixture of anatase / rutile or from amorphous-to-anatase-to-rutile in a simple and efficient manner.

This invention proposes a patterned PEDOT:PSS hydrogel based on electrochemical pulse deposition and its preparation method. The preparation apparatus includes an electrolytic cell, an electrochemical workstation, a substrate, a conductive layer, and a sacrificial metal layer. The electrolytic cell contains a PEDOT:PSS aqueous solution. The conductive layer is connected to the working electrode of the electrochemical workstation. Under the excitation of the electrochemical workstation, the sacrificial metal layer forms metal cations, which electrostatically adsorb PEDOT:PSS in the PEDOT:PSS aqueous solution, ultimately forming a patterned shape on the conductive layer using the sacrificial metal layer as a template. This invention achieves thickness control of the patterned PEDOT:PSS hydrogel by adjusting various deposition parameters. The preparation process is simple, low-cost, and highly practical, fully realizing the patterning of PEDOT:PSS hydrogels.

This invention provides a titanate hydrogel preparation device with an auxiliary structure, relating to the field of titanate hydrogel preparation technology. The device includes: a base; the base is placed on the ground, with two first connecting blocks symmetrically welded to its top surface, and a mixing chamber rotating on the two first connecting blocks; a base block is fixed to the top surface of the base, and a second connecting block slides on the base block. A motor drives a drive block to rotate, causing the mixing chamber to reciprocate and oscillate through compression, achieving preliminary mixing of the liquid macroscopically; a mixing plate inside the mixing chamber slides on a guide rod, and as the mixing chamber oscillates, the mixing plate gradually moves to the left, performing secondary stirring and mixing of the liquid; a mixing pipe in the mixing mechanism is connected to an external air supply pump, and gas is discharged through an annular array of vents at a 45-degree angle, which not only assists in mixing the liquid but also uses the reaction force of the gas jet to drive the mixing plate to move to the right, achieving further mixing of the liquid.

The application discloses a preparation method of a progesterone vaginal gel, relates to the technical field of gynecological medicine preparation, and realizes the preparation method of the progesterone vaginal gel by using a preparation device of the progesterone vaginal gel. The preparation device of the progesterone vaginal gel comprises a mixing kettle, a top plate is fixedly arranged at the top of the mixing kettle, a driving scraping mechanism is arranged inside the mixing kettle and at the inner side of the top plate, an oil phase preparation mechanism is arranged outside the driving scraping mechanism, a water phase preparation mechanism is arranged at the bottom outside the oil phase preparation mechanism, a premix preparation mechanism is arranged at the top outside the oil phase preparation mechanism, and the top plate and the water phase preparation mechanism are jointly provided with a driving supporting mechanism. The application can effectively shorten the transfer time of the premix and the oil phase, thereby improving the preparation efficiency of the gel product, and the residual oil phase can be recycled, thereby avoiding the waste of the oil phase and guaranteeing the quality of the gel product.

The invention relates to recyclable ionic gel with a microphase separation structure as well as a preparation method and application of the recyclable ionic gel, and belongs to the technical field of composite gel materials. The method comprises the following steps: dissolving an ionic liquid monomer containing unsaturated double bonds in an ionic liquid solvent, and uniformly mixing; and carrying out irradiation treatment on the solution to prepare the recyclable ionic gel with microphase separation. Inside the gel, alkyl chains tend to aggregate to reduce contact with a polar ionic environment. Non-polar alkyl chains are gathered to form an alkyl-rich region, anions and cations form an ion-rich region, and micro-phase separation is synergistically induced. The ionic gel prepared by the invention has the characteristics of excellent mechanical property, recoverability and the like, and effectively solves the problems of poor mechanical property and the like of the traditional non-covalent ionic gel.

The invention discloses a silver nanoparticle / polyvinyl alcohol hydrogel material, a preparation method thereof and application of the silver nanoparticle / polyvinyl alcohol hydrogel material in antibiotic detection. The silver nanoparticle / polyvinyl alcohol hydrogel material comprises monodisperse silver nanoparticles (AgNPs) and polyvinyl alcohol hydrogel, the monodisperse silver nanoparticles are embedded into the polyvinyl alcohol hydrogel, and an AgNPs / PVA composite substrate is formed through calcium ion induced aggregation and freeze thawing crosslinking. The silver nanoparticle / polyvinyl alcohol hydrogel material is used as a hydrogel SERS (Surface Enhanced Raman Scattering) chip for detecting residual veterinary drugs in animal-derived foods, shows good linear response to different antibiotics, has an adding standard recovery rate of 76-86%, is low in cost, and has extremely high sensitivity and relatively strong reproducibility, stability and uniformity.

The invention relates to a high-strength high-temperature-resistant cementing material and a preparation method thereof, belongs to the technical field of cementing materials, and solves the problems of complex preparation process, low strength, easiness in bleeding and segregation, poor high temperature resistance and the like of the traditional cementing material. The preparation method comprises the following steps: stirring silica sol to uniformly disperse the silica sol; adding a silane coupling agent into the silica sol, and stirring until the materials are uniformly mixed to obtain a first mixed solution; adding the first enhanced raw material powder into the first mixed solution, and stirring until the first enhanced raw material powder is uniformly dispersed to obtain a second mixed solution; adding the second enhanced raw material powder into the second mixed solution, and stirring until the second enhanced raw material powder is uniformly dispersed to obtain a third mixed solution; adding a gel accelerator into the third mixed solution, and stirring to obtain sol; after the sol is molded, standing and aging are conducted, and gel is obtained; and drying the gel in a drying oven to obtain the high-strength high-temperature-resistant cementing material. The high-strength high-temperature-resistant cementing material prepared by the method disclosed by the invention is good in integrity, excellent in mechanical property and good in high temperature resistance and freezing resistance.

The application provides a composite biochar material with a gradient pore structure and a preparation method thereof. The composite biochar material is obtained by high-temperature carbonization of a composite gel with a gradient pore structure, the composite gel with the gradient pore structure is obtained by centrifugal separation and directional photocuring, and the composite gel is composed of pre-activated dolomite and alkali pretreated soybean dregs. In the application, the density difference between the soybean dregs and the dolomite forms a gradient distribution of a layered structure during centrifugation, and the layered structure is solidified to maintain the gradient pore structure of the material in the subsequent carbonization process.

The invention relates to the field of catalysts, in particular to a multi-scale microporous catalyst and a preparation method thereof.Firstly, diffusion and mass transfer of reactants and products are greatly accelerated through a through multi-stage pore network of the catalyst, the exposure degree of active sites is remarkably increased, and therefore the catalytic efficiency is improved; secondly, according to the system, the pore structure and the material composition can be optimized and combined according to the characteristics of different catalytic reactions, such as reactant sizes, reaction media and conditions, so that the system can be widely adapted to various reaction types such as a gas state and a liquid state, and covers most of catalytic scenes; furthermore, various preparation methods can be flexibly combined, especially the 3D printing technology is applied, so that design customization of multi-scale pores and the appearance of the catalyst is realized, and the preparation difficulty of a complex structure is greatly reduced; and finally, reasonable framework material selection and multistage pore design synergistically enhance the catalytic effect and inactivation resistance of the catalyst, so that the service life of the catalyst is prolonged compared with that of a traditional catalyst.

The invention relates to polyvinyl alcohol polynitrogen isoacrylamide cellulose hydrogel with an anisotropic structure as well as a preparation method and application of the polyvinyl alcohol polynitrogen isoacrylamide cellulose hydrogel, and belongs to the technical field of water catching materials. The preparation method of the hydrogel comprises the following steps: S1, adding polyvinyl alcohol, glutaraldehyde and lithium chloride into deionized water to obtain a mixed solution A; s2, adding the cellulose nanocrystal powder into deionized water to obtain a cellulose nanocrystal dispersion liquid; s3, adding N-isoacrylamide and N, N-methylene bisacrylamide into deionized water, so as to obtain a mixed solution B; s4, adding the mixed solution A and the cellulose nanocrystal dispersion solution into the mixed solution B, and reacting to obtain a mixed solution C; s5, ammonium persulfate, hydrochloric acid and tetramethylethylenediamine are added into the mixed solution C, freezing, low-temperature crosslinking and unfreezing are conducted, and then freezing-unfreezing circulation crosslinking is conducted; and finally, taking out, unfreezing and dialyzing. The hydrogel provided by the invention has a highly anisotropic structure and regular vertical pore channels.

The present application relates to a kind of core-shell hydrogel microsphere preparation device.The core-shell hydrogel microsphere preparation device includes microfluidic chip and hydrophobic connecting pipe, the microfluidic chip is equipped with core phase flow channel, shell phase flow channel, water same axis flow channel, oil phase flow channel, ball forming cavity and outlet flow channel, the end of the core phase flow channel and the shell phase flow channel meet, the meeting point is connected with the first end of the water same axis flow channel, the water same axis flow channel and the end of the oil phase flow channel meet in the ball forming cavity, the ball forming cavity is communicated with the first end of the outlet flow channel, the inner diameter of the ball forming cavity is 0.2~2 mm, the inner diameter of the outlet flow channel is 0.4~2 mm, the outer diameter of the hydrophobic connecting pipe is matched with the inner diameter of the outlet flow channel, the inner diameter of the hydrophobic connecting pipe is less than or equal to the inner diameter of the ball forming cavity, one end of the hydrophobic connecting pipe is inserted into the outlet flow channel.

The present application relates to in situ model modeling method, especially for a kind of non-invasive cervical cancer in situ model modeling method of mouse, including selecting several 8 weeks old BALB / c-nude mouse, all mice are divided into non-invasive group and operation group, the age, weight of two groups of mice are compared, and there is no statistically significant difference;Non-invasive group is widened with the cotton stick of diameter 3mm and wipes dry vagina, the tumor block with luciferase fluorescence is immersed in modified hydrogel, after heating and making modified hydrogel solidification, the tissue glue mixed with hard gypsum in modified hydrogel is dropped on the surface of modified hydrogel and quickly inserted into vagina, when there is obstruction, stop deepening, pause 30s and wait for tissue glue to be completely dry, when tumor block is fixed in cervix, it is modeling completion;Operation group first opens abdominal cavity, and tumor tissue of same size is sutured on cervix.This kind of non-invasive cervical cancer in situ model modeling method of mouse, using non-invasive method to replace operation method to establish non-invasive cervical cancer in situ model, can reduce the pain of mouse, reduce experimental difficulty.

The application provides a high-rigidity high-flow-rate polysaccharide gel microsphere, a preparation method and an application, and the method comprises the following steps: S1, dispersing a polysaccharide powder in water to perform heterogeneous modification; S2, emulsifying and preparing a ball after the modified polysaccharide is dissolved; and S3, activation and crosslinking of the modified polysaccharide gel microsphere. The application adopts heterogeneous modification, pre-introduces a certain amount of a crosslinking agent, forms high-rigidity microspheres through crosslinking after solidification into balls, the linear flow rate of the microspheres is above 4000 cm / h, the microspheres have high flow rate and good mechanical strength, and the demand of large-scale separation and purification can be met. In addition, the application adopts heterogeneous modification, simplifies the process step of pre-introducing the crosslinking agent, and the heterogeneous modification makes the modified sites uniformly distributed, is beneficial to controlling the modification degree, and is beneficial to forming a rigid skeleton in the crosslinking process. The crosslinking reaction in the method provided by the application can be continuously performed, the process complexity is reduced, the method has the characteristics of simple operation, and is easy to be enlarged for large-scale industrial production.