A method for screening dandelion salt-tolerant mutants
A mutant and dandelion technology, which is applied in the field of screening dandelion salt-tolerant mutants, can solve the problems that the screening research of dandelion salt-tolerant mutants has not yet been reported, and achieve the effects of stable mutants, wide application prospects and high efficiency
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Embodiment 1
[0030] Example 1 Screening of dandelion salt-tolerant mutants
[0031] The medium configuration of this embodiment:
[0032] Induction medium: containing 0.5mg / L 6-benzylaminopurine (6-BA), 0.2mg / L dichlorobenzyloxyacetic acid (2,4-D), 7g / L agar powder and 30g / L sucrose, pH value 5.7-6.0 MS medium.
[0033] Liquid differentiation medium: MS medium containing 1mg / L kinetin (KT), 0.3mg / L naphthaleneacetic acid (NAA), 30g / L sucrose, pH 5.7-6.0;
[0034] Solid differentiation medium: MS medium containing 1mg / L kinetin (KT), 0.3mg / L naphthaleneacetic acid (NAA), 7g / L agar powder, 30g / L sucrose, pH 5.7-6.0.
[0035] Rooting medium: MS medium containing 0.5mg / L naphthaleneacetic acid (NAA), 7g / L agar powder, 30g / L sucrose, pH 5.7-6.0.
[0036] Specific test method:
[0037] 1. Aseptic vaccine culture
[0038] Select plump seeds and treat them with 75% ethanol for 30 seconds, then wash them with sterile water for 3 times, soak them in 0.1% mercury chloride for 12 minutes, then wa...
Embodiment 2
[0053] Identification of embodiment 2 salt-tolerant plants
[0054] Salt tolerance identification of salt-tolerant plants was carried out by potting method with soil full salt content of 0.2%, 0.4%, 0.6%, 0.8%, 1.0% saline-alkali soil, and regenerated plants without mutagenesis and NaCl screening were used as controls , record the leaf length and leaf width of the plant before transplanting, record the survival rate, leaf length and leaf width of the plant again 30 days after transplanting, and observe the growth of the plant.
[0055] The results showed that in the treatment with 0.2% total soil salt content, there was basically no difference between the salt-tolerant plants and the control in terms of survival rate and growth; in the treatment with 0.4% total soil content, the survival rate of the control plants was about 80%, and the salt-tolerant plants were basically All survived, and there was no obvious difference between the two in terms of growth; in the treatment of ...
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