Method for cultivating millet mutant by using EMS mutagenesis

A mutant and seed technology, applied in the field of crop breeding, can solve the problems of long working cycle, heavy workload, and narrow diversity of genetic resources for excellent germplasm, achieve good comprehensive agronomic traits, short process time, and avoid bad gene linkage Effect

Pending Publication Date: 2019-03-01
甘肃省农业科学院作物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of breeding new varieties of broomcorn millet, we are also faced with the problem of narrow diversity of genetic resources. Using EMS mutagenesis technology to create new varieties of broomcorn millet is an effective and rapid breeding method, but this technology has not yet been used for EMS mutagenesis on broomcorn millet. Technology optimization system, the work cycle of traditional breeding is long and the workload is heavy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Step 1: Seed pretreatment: Select a number of plump seeds of the millet variety Longmi No. 9 with a germination rate of more than 98%, take 26 25ml beakers, and mark T1E1, T1E2, T1E3, T1E4, T1E5, T2E1, T2E2, T2E3, T2E4 respectively , T2E5, T3E1, T3E2, T3E3, T3E4, T3E5, T4E1, T4E2, T4E3, T4E4, T4E5, T5E1, T5E2, T5E3, T5E4, T5E5, CK, take 1000 grains of selected Longmi No. 9 seeds respectively in the beaker, and use Soak in an appropriate amount of pure water for 8 hours, after the treatment is completed, use filter paper to dry the water on the surface of the seeds for later use;

[0023] Step 2: Seed treatment: Add 10ml of EMS solutions with a concentration of 0.4%, 0.6%, 0.8%, 1.0%, and 1.2% into the beakers marked with E1, E2, E3, E4, and E5, and follow the label T1 , T2, T3, T4, and T5 beakers soaked the seeds for 8h, 12h, 16h, 20h, and 24h respectively. After the treatment time was completed, the seeds were taken out and rinsed with pure water for 3 times. Continue...

Embodiment 2

[0030] Step 1: Seed pretreatment: select a number of plump seeds of the millet variety Jinshu No. 8 with a germination rate of more than 98%, take 26 25ml beakers, and mark T1E1, T1E2, T1E3, T1E4, T1E5, T2E1, T2E2, T2E3, T2E4 respectively , T2E5, T3E1, T3E2, T3E3, T3E4, T3E5, T4E1, T4E2, T4E3, T4E4, T4E5, T5E1, T5E2, T5E3, T5E4, T5E5, CK, respectively take 1000 grains of selected Jinshu No. 8 seeds in the beaker, and use Soak in an appropriate amount of pure water for 8 hours, after the treatment is completed, use filter paper to dry the water on the surface of the seeds for later use;

[0031] Step 2: Seed treatment: Add 10ml of EMS solutions with a concentration of 0.4%, 0.6%, 0.8%, 1.0%, and 1.2% into the beakers marked with E1, E2, E3, E4, and E5, and follow the label T1 , T2, T3, T4, and T5 beakers soaked the seeds for 8h, 12h, 16h, 20h, and 24h respectively. After the treatment time was completed, the seeds were taken out and rinsed with pure water for 3 times. Continue...

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Abstract

The invention a method for cultivating millet mutant by using EMS mutagenesis, and belongs to the technical field of crop breeding. The method for cultivating the millet mutant by using the EMS mutagenesis comprises the following steps of seed treatment, seeding, statistics and screening. A novel germplasm screened by the mutagenesis does not need to pass field plant selection, a plant-to-row test, a strain comparison test and a regional test, the process time is short, the working amount is small, the phenomenon of bad gene linkage is prevented from occurring, and in addition, the novel germplasm has better comprehensive agronomic characters.

Description

technical field [0001] The invention discloses a method for cultivating proso millet mutants by EMS mutagenesis, and specifically belongs to the technical field of crop breeding. Background technique [0002] At present, the breeding of millet varieties mainly includes systematic breeding and cross-breeding. The systematic breeding method forms a group through repeated screening and comparison of an excellent mutant individual plant. The process of experimenting and eventually becoming a new variety is longer. However, when the hybrid breeding method is used to introduce high-quality genetic resources from wild broomcorn millet resources to cultivated broomcorn millet, it is often accompanied by bad gene linkage, which will destroy the best genetic composition of the good germplasm, and may bring unfavorable traits, and the final effect may still be unsatisfactory. EMS (Ethyl Methane Sulfonate, ethyl methane sulfonate) mutagenesis technology has been widely used in wheat, c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 董孔军杨天育何继红刘天鹏张磊任瑞玉
Owner 甘肃省农业科学院作物研究所
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