A method for breeding japonica rice resistant to imidazolinone herbicides

An imidazolinone and herbicide-resistant technology, applied in the field of rice breeding, can solve the problems of difficult popularization and application, and the comprehensive properties of materials are not very good, so as to shorten the time, solve the difficult problem of weedy rice control, and save costs.

Active Publication Date: 2021-05-07
SHANGHAI ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, most of the herbicide-resistant rice materials obtained in China are obtained by natural selection or mutation, but the comprehensive properties of the obtained materials are not very good, such as yield, rice quality, etc., and it is difficult to popularize and apply them in production.

Method used

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  • A method for breeding japonica rice resistant to imidazolinone herbicides
  • A method for breeding japonica rice resistant to imidazolinone herbicides
  • A method for breeding japonica rice resistant to imidazolinone herbicides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Obtaining the Japonica Rice Mutant “CL55” Resistant to Imidazolinone Herbicides

[0033] With reference to the method of Gu Jiaqing et al. (Gu Jiaqing et al., Shanghai Agricultural Journal, 2005, 21 (1): 7-11), 3000 seeds of Hudao No. 55 (crop selection of Shanghai Academy of Agricultural Sciences, passed Shanghai Variety Approval in 2011) Soak in distilled water for 24 hours. The temperature should not be too high to prevent germination. After draining the water, treat it with 0.6% EMS for 24 hours. During the treatment, keep stirring. The leaf stage was treated with the imidazolinone herbicide "Dou Xerox" water agent produced by Shandong Xianda Chemical Co., Ltd., and the treatment concentration was 8 g / mu. There was a phytotoxicity reaction 7 days after the spraying, and the seedlings' hearts and leaves began to turn yellow, most of them gradually rotted, and finally died. Two of them survived normally, and the seedlings were moved to the breeding field aft...

Embodiment 2

[0035] Example 2 Effects of different concentrations of imidazolinone herbicides on rice germination

[0036] Using "CL55" and its parent "Shangdao 55" as experimental materials, imidazolinone at different concentrations of 0.0%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 1.0% and 1.2% were studied. Effects of similar herbicides (volume ratio) on the germination rate of rice grains. The experiment was repeated 2 times. Select 100 full seeds of each material and put them into a petri dish covered with 2 layers of absorbent paper, add different concentrations of herbicides respectively, put them in a constant temperature incubator at 31°C, and count the seed germination after 5 days (Table 1) .

[0037] Table 1

[0038]

[0039]

[0040] The results showed that when the herbicide concentration started from 0.1%, the germination rate of herbicide-resistant and non-herbicide-resistant varieties began to differ. The herbicide variety "Hudao 55" did not germinate at this concentr...

Embodiment 3

[0041] Example 3 Effects of different concentrations of herbicides and different treatment periods on rice growth

[0042] Using "CL55" and "Shanghai Rice 55" as test materials, different concentrations of herbicides (40 g / mu, 60 g / mu, 80 g / mu, prepared in 30 kg of water) and different treatment periods (1 leaf 1 core, 2 leaves 1 core, 3 leaves 1 core) on rice growth. After soaking the seeds of the two test materials to accelerate germination, select the germinated seeds that have sprouted neatly and sow them evenly. The area of ​​the plot is 1.5m 2 . Choose a sunny day, and spray different concentrations of herbicides evenly on the surface of 1 leaf with 1 core, 2 leaves with 1 core, and 3 leaves with 1 core. After 2 days, start investigating the plant damage and dead seedlings. The results are shown in Table 2 below. Different concentrations of herbicides Effects of pesticides and different treatment periods on rice growth.

[0043] Table 2

[0044]

[0045]

[004...

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Abstract

The invention discloses a method for breeding japonica rice resistant to imidazolinone herbicides. The imidazolinone herbicide-resistant rice mutant CL55 is used as a parent to hybridize with high-quality and high-yielding conventional japonica rice varieties. High-yield and high-quality japonica rice varieties resistant to imidazolinone herbicides were bred by the combination of herbicide identification and screening techniques. Compared with CL55, this variety has greater improvement and improvement in agronomic traits, yield and quality. The method of the present invention adopts non-transgenic technology, and the imidazolinone herbicide-resistant japonica rice varieties bred can solve the problem of preventing and controlling weedy rice in direct-seeded paddy fields, reduce labor intensity, save production costs, and promote the development of light rice cultivation technology in China. develop.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for breeding japonica rice resistant to imidazolinone herbicides. Background technique [0002] Rice is the main food crop in my country and the world, and is the main source of calories for nearly half of the world's population. In my country, due to the lack of agricultural labor force, the rice planting method has changed from the traditional transplanting method to the direct seeding method, and the weedy rice has spread, and this phenomenon is becoming more and more serious. With the promotion and application of light-weight rice cultivation technology in the past ten years, the occurrence and damage of weedy rice have become more and more serious, almost all over the major rice-producing areas in my country, and have become one of the main problems restricting the promotion of light-weight rice cultivation technology. [0003] Weedy rice is similar to ordina...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04A01H5/00A01H6/46A01G7/06A01C1/00
Inventor 朴钟泽白建江杨瑞芳方军张才辉
Owner SHANGHAI ACAD OF AGRI SCI
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