The application is suitable for the field of biological detection, and provides a
cholesterol detection method based on polydopamine / β-
cyclodextrin modified micro-nano
optical fiber.The method comprises a narrow
line width erbium-doped
fiber light source, a multimode
fiber jumper, a sensing unit, a
spectrometer and a computer.The sensing unit is prepared by removing the
coating layer of the multimode
fiber through Miller
forceps and is made into a biconical fiber (
waist diameter 8 μm, length 12 mm) through
hydrogen flame tapering, and the surface is modified with a polydopamine / β-
cyclodextrin combination (
dopamine solution concentration 2 mg / mL, β-
cyclodextrin solution concentration 5 mg / mL).During detection, the
light beam of the
light source (center
wavelength 1550 nm) passes through the sensing unit, and due to the local
refractive index change, further causes the evanescent field distribution and leads to the
red shift of the characteristic
wavelength of the
signal light, the
wavelength drift amount is observed by the
spectrometer, and the data is transmitted into the computer for wavelength
demodulation to realize the quantitative analysis of
cholesterol.The method has the advantages of low cost, high sensitivity, strong anti-interference and integration by improving the
light source stability through the double-ring cavity light source, increasing the sensing area through the biconical fiber and using specific
coating recognition molecules, and provides a new technical scheme for
cholesterol detection.