Full-automatic cell non-staining image recognizing and counting method

Abstract

The invention discloses a fully automatic cell non-stained image recognition and counting method, which selects the image recognition method automatic cell counter, directly adds the cell suspension to be tested into the counting sheet and inserts it into the counter, and reduces the red light R of the photosensitive chip. , the receiving ability of green light G light intensity, the operation of enhancing the receiving ability of blue light B light intensity, and manual adjustment of exposure time and exposure gain, so that the background of the display is light blue, and the circular bright spot is the image of living cells, while The circular blue spot darker than the background blue is the image of dead cells. Through the analysis of cell image recognition software, accurate analysis data of cell activity of various sizes can be obtained. The invention can count the activity of the cells without the use of dyes, avoiding the potential toxicity of the cells to be tested by using trypan blue staining, the cells after detection can continue to survive, and the activity can be counted during the culture process. At the same time, the invention can realize non-toxic Stained real-time online cell counting can be widely used in the field of life science research.

Description

technical field [0001] The invention relates to a detection method, in particular to a fully automatic cell non-staining image recognition and counting method for detecting cell quantity and activity. Background technique [0002] Since the cells must maintain a certain concentration in order to have better activity during culture, the cells need to be counted several times according to the actual situation in the process of cell recovery, culture, and cryopreservation; at present, the commonly used cell counting methods include automatic image recognition method and electrical impedance counting method. [0003] The electrical impedance counting method is based on the detection of the resistance change caused by the cells suspended in the electrolyte solution when they pass through the counting channel, and the cell count and volume are determined. This method is called the electrical impedance method, also known as the Coulter principle. . [0004] Disadvantages of the e...

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problems of the traditional cell counting method of image recognition after staining, the toxicity to living cells, the inability to count online, and the inability to stain embryonic cells For subsequent culture and proliferation and the inability to judge the activity and quantity of cells with endocytic function, the purpose of the present invention is to provide a fully automatic cell non-stained image recognition and counting method, which avoids the potential toxicity caused by the use of trypan blue, It can not only count the activity of cells of various sizes, but also can count the activity of cells that cannot be stained with trypan blue, such as germ cells and embryonic cells, and can also conveniently achieve accurate online counting

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