Full-automatic cell non-staining image recognizing and counting method

A technology of image recognition and counting methods, applied in the field of detection, can solve the problems of inability to judge the activity and quantity of endocytic cells, culture and proliferation, embryonic cells, etc.
CN103146800BActive Publication Date: 2014-08-13广州博大博聚科技有限公司
1 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
广州博大博聚科技有限公司
Publication Date
2014-08-13

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a fully automatic cell non-stained image recognition and counting method, which selects the image recognition method automatic cell counter, directly adds the cell suspension to be tested into the counting sheet and inserts it into the counter, and reduces the red light R of the photosensitive chip. , the receiving ability of green light G light intensity, the operation of enhancing the receiving ability of blue light B light intensity, and manual adjustment of exposure time and exposure gain, so that the background of the display is light blue, and the circular bright spot is the image of living cells, while The circular blue spot darker than the background blue is the image of dead cells. Through the analysis of cell image recognition software, accurate analysis data of cell activity of various sizes can be obtained. The invention can count the activity of the cells without the use of dyes, avoiding the potential toxicity of the cells to be tested by using trypan blue staining, the cells after detection can continue to survive, and the activity can be counted during the culture process. At the same time, the invention can realize non-toxic Stained real-time online cell counting can be widely used in the field of life science research.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a detection method, in particular to a fully automatic cell non-staining image recognition and counting method for detecting cell quantity and activity. Background technique

[0002] Since the cells must maintain a certain concentration in order to have better activity during culture, the cells need to be counted several times according to the actual situation in the process of cell recovery, culture, and cryopreservation; at present, the commonly used cell counting methods include automatic image recognition method and electrical impedance counting method.

[0003] The electrical impedance counting method is based on the detection of the resistance change caused by the cells suspended in the electrolyte solution when they pass through the counting channel, and the cell count and volume are determined. This method is called the electrical impedance method, also known as the Coulter principle. .

[0004] Disadvantages of the e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More