Preparation method and product of freeze-thawed acellular laser microporous irradiated heterogeneous dermal matrix

Abstract

The invention belongs to the field of xenogenic acellular dermal matrixes and preparation methods thereof. The preparation method of a freeze-thawing xenogenic laser microporous irradiated acellular dermal matrix is characterized by comprising the following steps: chipping a skin graft of piglet skin, and drawing the skin graft; perforating with laser; cleaning by adopting a buffer solution; performing freeze-thawing, and adding the buffer solution which is enough to immerse the skin graft for ultrasonic oscillation so as to remove destroyed dead cells and cell debris to maintain the completeness of structure and base membrane in acellular derma; cleaning with purified water, adding sulfadiazine zinc less than or equal to 0.12mg / cm<2>, and freezing at minus 20 DEG C; and sealing, disinfecting and sterilizing. The freeze-thawing xenogenic laser microporous irradiated acellular dermal matrix prepared with the method is characterized in that: the thickness is between 0.3mm and 0.4mm, the pitch is between 1.0mm and 2.0mm, and the pore diameter is between 500um and 808um.

Description

technical field [0001] The invention belongs to the field of decellularized xenograft matrix and its preparation method. Background technique [0002] "Micro-skin + large allogeneic skin" technology is currently an effective means of repairing and covering wounds of patients with large-area deep burns in China. This technology can effectively use the patient's residual skin, combined with allogeneic skin transplantation to temporarily cover large-area deep burns. The final wound surface solves the problem of lack of skin plaques. However, because the expansion of dermal tissue cannot be synchronized with the formation of epidermal epithelialization, lack of dermal tissue, and the quality of skin healing can hardly meet the requirements of function and appearance. After wound healing, the patient has severe contracture of scars, poor skin elasticity, hyperplasia of scars, pain and itching, etc. Affect the appearance and function, the reason is related to the lack of dermis c...

AI Technical Summary

Problems solved by technology

[0003] There are various biological materials used for the treatment of burn wounds, such as heterogeneous skin, heterogeneous skin, various biofilms and synthetic collagen fiber membranes. The problem is that there is rejection and may carry potential pathogens; and the source of heterogeneous skin is Very difficult, various synthetic materials are expensive, and clinical applications are limited

[0004] The previous decellularization methods mainly include enzymatic digestion method and chemical detergent method, etc. These methods can easily cause damage to the three-dimensional structure of the dermis, reduce the physical properties of the dermis, and it is difficult to completely remove cell debris; use glutaraldehyde to increase the dermis Toughness, which will lead to the destruction of collagen denatured structure, increase the cytotoxicity of dermal scaffold, and also inhibit the growth of normal cells