Sample pretreatment method based on flow combination ICP-MS (Inductively Coupled Plasma Mass Spectrometry) single cell protein detection
A single-cell protein and ICP-MS technology, which is applied in the field of pretreatment of human peripheral blood samples, can solve the problems that pretreatment methods cannot be applied to the detection of metal-labeled cells, and achieve the advantages of easy quenching, simple operation and convenient processing Effect
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[0046] Such as figure 1 As shown, the steps of the sample pretreatment method based on flow cytometry combined with ICP-MS single-cell protein detection include the following:
[0047] (1) Processing and cryopreservation of human peripheral blood samples (extraction of mononuclear cells-cell viability staining-cell stimulation-cell fixation)
[0048] 1. Reagent preparation. a. Reagent equilibrated to normal temperature: Ficoll lymphocyte separation medium; Ca2+ / Mg 2+ Free PBS; 2×Fixation Solution (3.2% PFA in PBS); Human red blood cell lysate (optional); Cell Staining Buffer (0.5% BSA+0.02% NaN 3 in PBS). b. Reagents that need to be preheated: FBS Free DMEM / 1640 (37°C). c. Place the following reagents on ice: Cell Staining Buffer containing 10% DMSO; Cell Staining Buffer; Cisplatin 5mM;
[0049] 2. Collection and transportation of whole blood samples. Blood sample collection is an important part of quality control before analysis. It is recommended to use heparin or ci...
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