5results about How to "Stable secretion" patented technology

The application belongs to the technical field of biology and particularly relates to an African swine fever virus pNP868R protein monoclonal antibody and a preparation method and application thereof. The application takes the pNP868R protein prepared by eukaryotic expression as an antigen, immunizes BALB / c mice, uses hybridoma technology, and obtains a hybridoma cell line capable of stably secreting the anti-African swine fever virus pNP868R protein monoclonal antibody through cell fusion, indirect ELISA method screening, limited dilution method subcloning and hybridoma continuous passage culture. The application also obtains an African swine fever virus pNP868R protein monoclonal antibody. The monoclonal antibody has high affinity with the pNP868R protein and can be applied in Western-Blot experiments, indirect immunofluorescence experiments, immunoprecipitation experiments and the capture of proteins interacting with pNP868R, and can be used for the diagnosis, prevention or treatment of African swine fever.

This invention relates to a hybridoma cell line, its secreted mouse anti-human Hb monoclonal antibody, and its applications, belonging to the fields of immunology and in vitro diagnostics. A hybridoma cell line is deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: C2023339. The hybridoma cell line prepared by this invention can stably secrete mouse anti-human Hb monoclonal antibodies. The mouse anti-human Hb monoclonal antibodies secreted by the hybridoma cell line have characteristics such as small batch-to-batch variability and high affinity. The detection kit prepared using the mouse anti-human Hb monoclonal antibody secreted by the hybridoma cell line of this invention has a wide linear range, high specificity, and good positive / negative differentiation.

The application belongs to the technical field of biology, and discloses a hybridoma cell strain 2F8 secreting a calmodulin 2 monoclonal antibody, a specific monoclonal antibody, and preparation and application. The hybridoma cell strain 2F8 secreting the calmodulin 2 monoclonal antibody is classified and named as 2F8, and is preserved in the China General Microbiological Culture Collection Center on April 9, 2025, with a preservation number of CGMCC No.46343. The application also provides the calmodulin 2 monoclonal antibody secreted by the hybridoma cell strain 2F8 and a polynucleotide encoding the calmodulin 2 monoclonal antibody. The antibody has specific recognition ability for the calmodulin 2, and is suitable for immunological detection, in particular, Western-blot and immunohistochemical detection. The antibody can be used for preparing a reagent, a kit or other immunological detection product for detecting the calmodulin 2, and has practical application value.

This invention relates to the fields of microbial fermentation and genetic engineering technology, specifically disclosing a method for preparing human type III collagen fragments. Based on Pichia pastoris, this invention constructs a multidimensional synergistic expression system of "molecular chaperone co-expression - precursor supply - osmotic pressure adaptation regulation," reducing the structural instability of collagen fragments during secretion and post-processing, and constructing an expression system suitable for stable and efficient secretion of collagen fragments. The method described in this invention can achieve efficient extracellular secretory expression of human type III collagen fragments, and the obtained protein has high structural uniformity and stability, with a protein yield of up to 6.55 g / L under 5 L fermenter conditions. The method described in this invention is suitable for the large-scale preparation of human type III collagen fragments and provides a new technical solution for obtaining collagen products with high structural uniformity and good functional activity.

The application discloses a monoclonal antibody targeting all subtypes of CD45 and an application thereof, and belongs to the field of monoclonal antibody preparation. The application stably expresses shorter and longer molecular subtypes of an extracellular region of human CD45 in L929 mouse fibroblasts, immunizes BALB / c mice with L929 cells expressing both molecular subtypes of human CD45, fuses spleen cells with SP20 myeloma cells after the serum titer reaches a standard, successfully obtains a hybridoma cell strain stably secreting an antibody against all subtypes of human CD45, and obtains the monoclonal antibody targeting all subtypes of CD45. The monoclonal antibody provided by the application shows high affinity binding characteristics with all molecular subtypes of human CD45, has important application value in human CD45 molecular detection, and can be used as a therapeutic antibody in the fields of tumor immunotherapy, autoimmune disease treatment and anti-transplant rejection and the like.