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Muscovy duck parvovirus inactivation vaccine and application thereof

A technology of muscovy duck parvovirus and inactivated vaccines, which can be used in antiviral agents, medical preparations containing active ingredients, antibody medical ingredients, etc., can solve the problems of reduced vaccine potency, achieve good safety, and prevent ducklings Effects of parvovirus infection

Active Publication Date: 2015-06-10
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, due to the reduction of the potency of the vaccine used due to virus mutation, it is also necessary to screen out the mutated virus strain to prepare a vaccine with higher efficacy

Method used

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  • Muscovy duck parvovirus inactivation vaccine and application thereof
  • Muscovy duck parvovirus inactivation vaccine and application thereof
  • Muscovy duck parvovirus inactivation vaccine and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1. Screening of YBMDV strains

[0015] In 2011, an acute Muscovy duck parvovirus disease broke out from a duck farm in Zhejiang Province. The pancreas and intestines of the dead ducks were taken, and the pancreas and intestinal tissues were homogenized with non-physiological saline to make a 20% suspension, 3000r / Centrifuge for 15 min, take the bacteria removed, inoculate 13-day-old Muscovy duck embryos through the allantoic cavity respectively, incubate for 120 hours, collect the allantoic fluid and embryonic body tissue, after homogenization, freeze and thaw three times repeatedly, take the supernatant Freeze. The harvested virus liquid was purified and analyzed and tested for virus characteristics in terms of virus content, immunogenicity, specificity and purity. The results showed that the virus content of this strain was 10%. 5.7 ELD 50 / 0.2ml, the minimum immune dose is 10 2.0 ELD 50 / 0.2ml, the virus only reacts specifically with Muscovy duck parvovi...

Embodiment 2

[0019] Example 2 Preparation of vaccine

[0020] 1. Preparation of the virus liquid for seedling production The YBMDV strain of the virus seed for production was inoculated into the allantoic cavity of 12-14-day-old susceptible Muscovy duck embryos, 0.2 ml per embryo, incubated at 36-37 °C, and the embryos were examined twice a day. . Select embryos that die within 48-120 hours after inoculation, and have extensive hemorrhage in embryos, hemorrhage of hair follicles, liver degeneration and necrosis, and mild edema of choriouretic membranes. Take the allantoic fluid and embryos and put them in a tissue masher Pulverize, take the pulverized tissue and add it to normal saline at a ratio of 1:2 to mix; set it at 2-8°C for 4-12 hours, collect the allantoic fluid, amniotic fluid and embryos (remove the head and limbs). Homogenize with embryonic fluid, freeze and thaw for 3 times, centrifuge at 4000 r / min for 30 min, take the supernatant, mix it in a sterile container, and store at ...

Embodiment 3

[0044] 1. Preparation of antigens for seedling production

[0045] 1. Preparation of virus liquid for seedling production Inoculate 12-14-day-old susceptible muscovy duck embryos into the allantoic cavity of the virus species YBMDV strain for production, 0.2ml per embryo, incubate at 36-37°C, and inspect the embryos twice a day . Muscovy duck embryos that died within 48 to 120 hours after inoculation, and embryo bodies with extensive hemorrhage, hair follicle hemorrhage, liver degeneration and necrosis, and chorioallantoic membrane with mild edema were selected, and the allantoic fluid and Muscovy duck embryo bodies were put into Crush the tissue in a masher, take the crushed tissue and mix it with normal saline at a ratio of 1:2; place it at 2-8°C for 4-12 hours, and collect the allantoic fluid, amniotic fluid, and embryo body (remove the head and limbs). Homogenize with embryo fluid, freeze-thaw 3 times, centrifuge at 4000r / min for 30min, take supernatant and mix it in a st...

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Abstract

The invention provides a Muscovy duck parvovirus inactivation vaccine which comprises an antigen and a vaccine adjuvant, wherein the antigen is inactivated Muscovy duck parvovirus, and the collection number of the Muscovy duck parvovirus is CGMCC No.8504. The Muscovy duck parvovirus inactivation vaccine provided by the invention is prepared by the steps of screening a Muscovy duck parvovirus YBMDP strain with low toxicity and high immunogenicity; inoculating and collecting the virus liquid; performing ultrafiltration concentration and formaldehyde solution inactivation; adding an oil adjuvant and mixing and emulsifying to obtain the vaccine. The vaccine provided by the invention can improve the antibody level of Muscovy duck, guarantee the maternal antibody level of the filial generation and prevent the duckling parvovirus infection caused by the Muscovy duck parvovirus; the vaccine has the advantages of high efficiency and good safety.

Description

technical field [0001] The invention belongs to the technical field of poultry pathogenic microorganism screening, and in particular relates to a muscovy duck parvovirus inactivated vaccine and application thereof. Background technique [0002] Muscovy duck parvovirus disease is an acute or subacute infectious disease affecting young muscovy ducks, characterized by panting, diarrhea, pancreatic necrosis and hemorrhage; it is highly contagious, with high morbidity and mortality. Very high, often causing serious economic losses. The disease mainly occurs in Muscovy ducklings of 1 to 3 weeks old, especially Muscovy ducklings about 10 days old, and basically no disease when they reach the age of 3 weeks or more. The disease first appeared in the mid-to-late 1980s in Fujian, China and the Brittany region in western France. It was only recognized in the early 1990s that it was an independent disease different from gosling plague. If the dead muscovy duck is not handled properly,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/23A61P31/20
Inventor 李陆梅宫晓徐保娟王红宋新宇邹敏李芳
Owner YEBIO BIOENG OF QINGDAO
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