89 results about "Raccoon Dogs" patented technology

The invention discloses a complex additive for foxes, raccoon dogs and martens, which pertains to the technical field of animal nutrition. In the invention, a variety of vitamins and microelements which are required to promote the reproductive performance of the foxes, the raccoon dogs and the martens are strongly strengthened, and the balance of all nutritional factors are also synthetically considered; the additive is added with high-quality yolk powder, active microzyme and complex enzyme preparation; the components and contents of each kilogram of the additive are restricted within the best range. The basic nutrition requirements of the foxes, the raccoon dogs and the martens at the breeding stage are fully considered in the invention, and a plurality of nutrient factors which affect the reproductive performances of the foxes, the raccoon dogs and the martens are also emphasized. By adopting the additive, the breeding animals can reach good posture, the development of the generative organs is promoted, the estrus expression is obvious, the hybridization time is concentrated and advanced, the birth amount and ablactation survival rate are improved, and the barren rate and dystocia rate are reduced, thus effectively improving the reproductive performance of the foxes, the raccoon dogs and the martens.

The invention provides a feed additive for improving the quality of the fur of a furry animal. The additive is obtained by mixing a semi-finished vitamin product, a semi-finished trace element product, dihydropyridine, methionine, cystine and corn gluten meal according to a weight ratio of 1:1:0.3-1:0.2-0.9:0.1-0.6:2, wherein the semi-finished vitamin product is prepared by uniformly mixing the following components: 5-22 parts of vitamin A, 3-8 parts of vitamin D, 10-25 parts of vitamin E, 3-8 parts of vitamin B1, 8-15 parts of biotin, 1-3 parts of vitamin B6, and 120-160 parts of the corn gluten meal, and the semi-finished trace element product is prepared by uniformly mixing the following components: 10-20 parts of methionine chelated zinc, 2-5 parts of methionine chelated copper, and 70-90 parts of zeolite powder. The additive of the invention allows the quality of the fur of the furry animals (martens, foxes and raccoon dogs) to be substantially improved, the hair density to be increased, the hair length to be improved, the hide toughness to be increased and the culture benefit to be improved.

The invention discloses an animal-derived component detection kit. The kit comprises a primer combination of at least three primer pairs in primer pairs disclosed as SEQ ID NO.1-22. The kit can be used for detecting components derived from pigs, cattle, raccoon dogs, sheep, chickens, ducks, rabbits, foxes, minks, donkeys and mice, and has the characteristics of high specificity, high sensitivity and high detection result visualization degree. The animal-derived component detection kit can be judged directly with the naked eyes, is low in cost, and thus, is suitable for large-scale detection on animal-derived components of various foods.

The invention discloses immune colloidal gold test paper capable of simultaneously detecting canine distemper and canine parvo of foxes, raccoon dogs and minks and a method for preparing the immune colloidal gold test paper. The test paper consists of a test paper box and a test paper tape, and the test paper tape is made by sequentially bonding a hard polyvinyl chloride lining plate, a nitrocellulose membrane, a long colloidal gold combination pad, a short colloidal gold combination pad, a sample pad and an absorption pad. VP2 gene monoclonal antibody- colloidal gold biomarkers resistant to mink-source canine distemper viruses and parvoviruses are sprayed on the long and short colloidal gold combination pads respectively. Two detection lines formed by rabbit anti-mink source canine distemper virus and parvovirus antibodies and a quality control line formed by a rabbit antimouse antibody are sprayed on the nitrocellulose membrane. The test paper is applicable to quick detection of pestilences of foxes, raccoon dogs and minks, and detection of two pestilences including canine distemper and canine parvo of foxes, raccoon dogs and minks can be simultaneously completed by the same test paper, and detection is simple, convenient, quick and accurate.

The invention relates to a manufacturing method for animal feed, in particular to a manufacturing method for a special animal feed exclusively used in fox, mink and raccoon dog. The method has the advantages that: raw materials of corn and soybean meal required in the formula are bulked by a dry method or wet method and ground; the ground material is mixed with meat and bone meal and imported fishmeal, and then bulked again (by the dry method or wet method); the second bulking is completely different from other bulking methods for an annular space discharging mode is adopted in the discharging in front of a bulking machine in the second bulking and the bulking temperature is 100-130 DEG C, which not only realizes the aim of sterilization and curing, but also avoids breaking the nutritional factors of the materials due to over-high temperature; the product produced by the process has high bulking degree, good water binding capacity, good palatability and thorough sterilization. The premix materials are added and mixed twice to avoid reducing the effect caused by heating.

The invention relates to a composite premix feed for foxes and raccoon dogs. The composite premix feed is applied to the field of fox and raccoon dog breeding or the field of fur animal feed production and is particularly used for improving the productivity of the foxes and the raccoon dogs. The composite premix feed comprises formulas for the growth period and the reproduction period. The formula mainly comprises multi-vitamin, blue copperas, baile copper, ferrous sulfate monohydrate, baile iron, zinc sulfate monohydrate, baile zinc, manganese sulfate monohydrate, baile manganese, potassium iodide, sodium selenite, aureomycin, choline, angel active dry yeast, angel composite yeast, calcium hydrophosphate and corn protein powder. Composite premix feed constituents and contents of the constituents for two key production stages, namely the growth period and the reproduction period, of the foxes and the raccoon dogs are researched; the growth performance of the foxes and the raccoon dogs is improved, and the reproduction performance of seed beast is improved.

The invention relates to an efficient-secretion canine parvovirus (CPV) resistant monoclonal antibody hybridoma cell A135 strain and belongs to the technical field of biology. The hybridoma cell A135 strain selected from the secretion CPV resistant monoclonal antibody hybridoma cell bank is excellent in biological performance and is injected with ascetic fluid generated in a BALB / C mouse peritoneal, so that neutralizing titer is up to 1010. In addition, the hybridoma cell A135 strain has moderate neutralizing capacity for various CPV subtype virus strain such as CPV-2a, CPV-2b, CPV-2c (a) and CPV-2c (b) and CPV strains from foxes and raccoon dogs, is wide in anti-CPV strain range and is used for clinical treatment of attacked dogs with CPV diseases, and effective rate is up to 100%.

The invention relates to a refined forage for enhancing the immunity of raccoon dogs. The refined forage comprises the following raw materials, by weight, 40-45 parts of corn, 20-25 parts of soybean meal, 20-25 parts of fish meal, 3-5 parts of blood powder, 3-5 parts of bone meal, 1-3 parts of edible oil, 1-2 parts of lysine, 1-2 parts of threonine, 0.5-1 part of calcium hydrogen phosphate, 0.5-1 part of a composite vitamin, 0.1-0.5 parts of composite trace elements, 0.5 parts of salt, and 0.05-0.1 parts of spirulina. The refined forage is obtained through mixing and uniformly stirring the raw materials. The forage has the advantages of scientific compatibility, scientific calculation in proportioning, overcoming of the disadvantages comprising single nutrition and bad disease resistance of present forages, shine fur color and uniform and thick under hair of raccoon dogs, and improvement of the quality of the raw material for fur products.

The invention relates to a preparation method for obtaining blood used for preparing homologous antiserums and a spleen byproduct used for preparing transfer factors from the bodies of fur bearing animals such as foxes, raccoon dogs and minks; the furs of the foxes, the raccoon dogs and the minks are taken concentratedly in December and confirmed according to the fur-taking dates of different areas; healthy individuals are picked up 45 days before a predicated fur-taking date; the booster immunization of univalent vaccines or polyvalent vaccines of canine distemper, Canine parvovirus enteritis, adenovirus disease, corona virus laxness, parainfluenza, and pasteurellosis is carried out for 3 times by purified and condensed antigens on the foundation of carrying out immunization for once in summer; when the furs are taken, aseptic anticoagulation blood collection is carried out to a heart to improve the blood serum yield; and the spleens of the animals are collected for extracting specific or common transfer factors simultaneously. The blood can be conveniently prepared into the blood serums which resist communicable diseases, and the transfer factors can be extracted the spleen, thus providing guarantee for the healthy development of the breeding in the next year; the invention is used for curing the corresponding communicable diseases of the wild animals of the same species; and when a small amount of communicable disease cases appear in a breeding crowd, the corresponding pathogeny hyper-immune serums and transfer factors are mainly injected to a supposed healthy crowd after the pathogeny is confirmed so as to cure the affected animals in the early period of disease and control the spreading of the epidemic situations.

The invention relates to a compound premixed feed capable of promoting the growth and improving the hide length of a racoon dog in winter hair growth period. Every 1kg of the compound premixed feed is prepared from the following components by weight: 10-25g of vitamin, 10-20g of compound enzyme, 10-24g of amino acid, 3.0-25g of trace elements, 15-35g of beneficial bacteria, 41-62g of nutritional agents, 200-300g of anti-diarrhea drugs and the balance being rice hull powder. According to the long-term feeding tests, the compound feed disclosed by the invention has effects of promoting the growth, achieving the health care, lowering diarrhea and improving the hide length, so that the best growth performances of the raccoon dog are achieved in the winter hair growth period.

The invention relates to a strengthening agent for reproductive capacity of foxes and raccoon dogs, which belongs to the field of animal nutrition and reproductive technology.Every 100g product consists of 20-30g of soybean flour, 15-20g of fish powder, 8-12g of algae powder, 10-15g of yolk powder, 3-8g of Chinese onion seed powder, 2-6g of Chinese chive seed powder, 0.8-1.5g of linoleic acid, 100-500mg of vitamin E, 100-200mg of methionine manganese, 300-800mg of methionine zinc, 50-100mg of lysine selenium and 1.5-4.0g of vitamin complex , the rest is corn-meal. The invention can supply the foxes and raccoon dogs with sufficient nutrition, improve development of reproductive organs of the foxes and raccoon dogs, promote secretion of androgen, increase sperm motility, survival rate and fertilization rate, reduce sperm abnormality rate and enhance reproductive capacity of male foxes and raccoon dogs.

The invention relates to a Chinese medicinal composition for treating diarrhea of foxes, raccoon dogs and martens. The Chinese medicinal composition is prepared by smashing and mixing the following Chinese medicines in parts by weight: 5-10 parts of Chinese pulsatilla root, 2-8 parts of baikal skullcap root, 20-40 parts of pomegranate peel, 8-20 parts of red halloysite, 5-20 parts of fried dark plum fruit and 15-30 parts of liquoric root. The Chinese medicinal composition disclosed by the invention is mainly used for clearing away heat and toxic materials, stopping dysentery and thickening intestinal tracts, is easy to prepare, is low in cost, and is safe and reliable; and after foxes, raccoon dogs and martens are fed with the Chinese medicinal composition, symptoms are relieved rapidly, quick recovery is realized, and a remarkable curative effect is achieved.

The invention belongs to the technical field of textiles and relates to a production method of blended fabric of water-soluble fiber and racoon dog fur. A technical process of mixing, carding, spinning, spooling, plying and double-twisting is provided; water-soluble fiber is added to raccoon dog fur in a certain ratio, so that fewer broken ends occur during spinning of racoon dog fur products, andspinnability is improved. In addition, finished textile fabric finally made can retain the original style of raccoon dog fur products after special afterfinishing and removal of the water-soluble fiber in the textile fabric.

The invention discloses a special anti-wilt biological organic fertilizer for strawberries prepared from mink, fox and raccoon dung and a preparation method thereof. The specific anti-wilt biologicalorganic fertilizer is prepared by taking the fresh mink, fox, raccoon dung and corn straw as stacking materials and inoculating a composite microbial fermentation agent consisting of bacillus subtilisABTNL-2, bacillus thuringiensis ABTNL-4, Herbaspirillum huttiense ZL-3, bacillus megaterium and bacillus mucilaginosus and bacillus licheniformis ABTNL-1 biocontrol agent through a one-step through composting fermentation process to obtain the biological organic fertilizer with anti-strawberry wilt function. According to the invention, a static aerobic composting system is established to realizeharmless treatment and resource utilization of agricultural wastes, and the Bacillus licheniformis which has an obvious inhibiting effect on strawberry wilt and the one-step through composting fermentation process are connected, so that the special anti-wilt bio-organic fertilizer for strawberries is prepared, and the production process of the bio-organic fertilizer is optimized to reduce the production cost.

The invention discloses a raccoon dog distemper freeze-dried live vaccine and a preparation method and application thereof and belongs to the technical field of biological products for animals. The effective component of the raccoon dog distemper freeze-dried live vaccine is a raccoon dog distemper virus attenuated vaccine strain. The attenuated vaccine strain is a dog distemper attenuated vaccine strain which is obtained through acclimatization on a dog distemper vaccine strain CDV-O2, and is safe to a raccoon dog, good in immunogenicity and stable in titer, the attenuated vaccine strain is named as CDV-OR12 strain, and the microbial preservation number of the attenuated vaccine strain is CGMCC NO. 13793. Experiments verify that the vaccine can effectively prevent the raccoon dog from being attacked by dog distemper virus virulent viruses, the protection rate is more than 90 percent, raccoon dog distemper can be effectively prevented, and the immunity period is 6 months. A new technical way for preventing and controlling raccoon dog distemper is provided.

The invention provides a feed for supplementing calcium for a fox, a raccoon dog and a marten. The feed is prepared from the following raw materials in parts by weight: 20-40 parts of calcium-containing additive, 10-30 parts of cane sugar, 3-8 parts of taurine and 30-70 parts of auxiliary materials. The calcium-containing additive is a mixture of calcium lactate and calcium chloride, and the auxiliary materials are at least one of mountain flour, zeolite powder and maifanite. The feed for supplementing the calcium has the characteristics of high absorption speed and high bioavailability, can be effectively prevented from being corroded by gastric acid, is beneficial to the increase of the stability, and can be widely applied to the culture of the fox, the raccoon dog and the marten.

The invention relates to a compound feed for foxes and raccoon dogs, which comprises the following components in percentage by weight: 30-40% of corn meal, 15-25% of bean pulp, 20-25% of fish meal, 5-8% of blood powder, 3-5% of oil, 1-3% of amino acid, 0.5-1% of table salt, 5-10% of calcium lactate, 3-5% of calcium chloride, 1-3% of taurine and 1-5% of premix. A preparation method comprises the step of uniformly mixing the components according to the ratio to obtain a finished product. According to the compound feed for foxes and raccoon dogs, the added calcium lactate and calcium chloride do not consume gastric acid in the animal bodies, are high in absorption degree, effectively avoid feed erosion caused by gastric acid, are good in mouth feeling, and provide sufficient calcium for the animal growth; taurine participates in maintaining the environment homeostasis regulation in the organism, and has important regulation effect on the normal playing of physiological functions; the microelements in the premix can improve the disease resistance of the foxes and the raccoon dogs.

The invention relates to a premixed feed stuff for fox and raccoon dog containing organic microelements including ferric methionine, Zinc-methionine, Copper methionine and Manganese methionine, the charging amounts include (calculated by the tracer element contents): ferrum 100-200mg / kg, zinc 100-220mg / kg, copper 10-50mg / kg, manganese 10-80mg / kg, the inorganic microelements include ferric persulfate, zinc sulfate, copper sulphate and manganese sulphate, the charging amounts include (calculated by the tracer element contents): ferrum 500-1000mg / kg, zinc 500-1100mg / kg, copper 50-250mg / kg, manganese 50-400mg / kg.

The invention relates to a premixed feed stuff for pigs containing organic microelements including ferric methionine, Zinc-methionine, Copper methionine and Manganese methionine, the charging amounts include (calculated by the tracer element contents): ferrum 100-200mg / kg, zinc 100-220mg / kg, copper 10-50mg / kg, manganese 10-80mg / kg, the inorganic microelements include ferric persulfate, zinc sulfate, copper sulphate and manganese sulphate, the charging amounts include (calculated by the tracer element contents): ferrum 500-1000mg / kg, zinc 500-1110mg / kg, copper 50-250mg / kg, manganese 50-400mg / kg.

The invention relates to a kit for detecting a canine distemper virus blocking ELISA (Enzyme Linked Immunosorbent Assay) antibody of a mink, a fox and a raccoon dog. The kit is characterized by comprising an ELISA batten, a blocking antibody, an enzyme-labeled antibody, a substrate color developing solution, a stop solution, positive control, negative control and ELISA envelope antigen, wherein the ELISA envelope antigen adopts the canine distemper virus with the preservation number of CGMCCNO.4858. The kit for detecting the canine distemper virus blocking ELISA antibody which is developed by using an ELISA technology provides a detection method which is accurate, rapid and good in safety, and can be used for screening a large number of canine distemper virus antibodies.

The invention provides an addition and production method of poultry butts into feed for foxes or raccoon dogs in winter fur period. The addition and production method is characterized in that: grain raw materials are selected, puffed and crushed into particles of 40 mesh, animal raw materials are selected and crushed into particles of 40 mesh, trace elements are premixed, and the three types of the above raw materials are mixed; the mixture is secondarily micronized, namely the mixed raw materials are crushed into particles of 80 mesh and then subjected to seasoning, puffing and granulating, and cooling, and vitamin medicines are prepared into a premix and sprayed on the particle feed; finally the particle feed is screened and packaged. The fatty acids of the feed for foxes or raccoon dogs in winter fur period are balanced, and the quality of fur of foxes or raccoon dogs is improved. The cost of feed for foxes or raccoon dogs is greatly reduced. The pure protein utilization rate and nitrogen biological value are increased.

The invention relates to a raccoon dog parvoviral enteritis and canine distemper combined inactivated vaccine and a preparation method thereof. The raccoon dog parvoviral enteritis and canine distemper combined inactivated vaccine comprises the effective ingredients of mink parvoviral VP2 protein, acquired by culturing recombinant rhabdovirus RDPV-SN1 transfected Sf9 cells, and a canine distempervirus inactivated antigen. In order to solve the problem that the racoon dog and canine distemper and parvovirus combined active vaccine has the risk of virulence return, while the combined inactivated vaccines used in the market generally cause low immunity after the raccoon dog parvovirus is inactivated, the invention provides a combined inactivated vaccine prepared with raccoon dog parvoviral VP2 protein and the canine distemper virus inactivated antigen having high viral content. The combined inactivated vaccine herein is applicable to the prevention of raccoon dog parvoviral enteritis andcanine distemper, has the advantages of good immunogenicity, high safety, high controllability, high culture efficiency, high automation level, large-scale production and the like, can provide doublepreventions and has a good application prospect.

The invention discloses a raccoon dog hair fiber bleaching and decoloration method. The technology includes the first step of degreasing, the second step of pretreatment, the third step of cleaning, the fourth step of bleaching, the fifth step of cleaning, the sixth step of dip bleaching and the seventh step of drying in the sun. The raccoon dog hair fiber bleaching and decoloration method has the advantages that the raccoon dog hair fiber bleaching and decoloration technology is simple, damage to raccoon dog hair fibers is small under the condition that whiteness is guaranteed, and the hand feeling of the raccoon dog hair fibers is soft.

The invention discloses a fluorescent PCR (polymerase chain reaction) detection method, an amplifier and a probe for raccoon dogs. The fluorescent PCR detection method includes supplementing water into 10 microliters of Premix Ex Taq which is a real-time fluorescent PCR system, 0.4 microliter of upstream primers, 0.4 microliter of downstream primers, 0.4 microliter of the probe and 1 microliter of sample DNA (deoxyribonucleic acid) liquid obtained at a step 1 to obtain 20 microliters of liquid; carrying out reaction programs (1) at the temperatures of 95 DEG C for 10 sec, reaction programs (2) at the temperatures of 95 DEG C for 5 sec and reaction programs at the temperatures of 60 DEG C for 23 sec by the aid of a fluorescent quantitative PCR instrument; carrying out 40 cycles. The fluorescent PCR detection method, the amplifier and the probe have the advantages that endogenous components of the raccoon dogs can be accurately and quickly identified by the aid of the primers and the probe which are high in specificity and sensitivity, and the fluorescent PCR detection method, the amplifier and the probe can be effectively used for identifying the authenticity of meat products, fur and the like and can assist relevant departments to crack down severe behavior of meat and meat product fake production and sales.

The invention provides raccoon dog parvovirus VP2 gene, an expression vector, a recombinant bacterium, a method for preparing VP2 protein and an assembly method, and belongs to the technical field ofvaccine preparation. The raccoon dog parvovirus VP2 gene has a nucleotide sequence shown as SEQ ID No. 1. According to the invention, the raccoon dog parvovirus VP2 gene and a molecular chaperone arejointly transformed into ER2566 cells, and the molecular chaperone improves the correct folding rate of VP2 protein. The raccoon dog parvovirus VP2 gene provided by the invention can form high-qualityraccoon dog parvovirus-like particles. A raccoon dog parvovirus-like particle vaccine provided by the invention has a hemagglutination titer of 216 after being purified, and has a hemagglutinating property similar to that of a natural virus.

The invention relates to a traditional Chinese medicine composition for treating fox raccoon dog distemper. The traditional Chinese medicine composition is prepared by ephedra, cassia twig, almonds, liquorice, fagopyrum cymosum, gypsum, ophiopogon, pinellia ternate, ginseng, date, root-bark of tree peony, radix puerariae, fritillaria cirrhosa and asarum. The traditional Chinese medicine composition can be powder. The preparation method of the traditional Chinese medicine composition comprises the following steps: (1) selecting: selecting out all impurities, stones and metal, and removing non-medicinal parts; (2) elutriating: enabling 2-3 times of water to slowly enter medicine by using an infiltration method, so as to ensure that the medicine is infiltrated, and the water is used up; (3) drying: reducing the pressure and drying at a temperature of 40-60 DEG C until complete drying; (4) crushing: slightly crushing various components respectively and screening by using a 600-mesh screen; (5) mixing: weighing according to the weight and uniformly mixing for 10-20 minutes; (6) packaging into bags: ensuring that the weight of each bag is 100-200 g so as to obtain a finished product. The traditional Chinese medicine composition and the preparation method thereof have the benefits that the traditional Chinese medicine composition has the effects of inducing diaphoresis and relieving exterior syndrome, lifting Yang and stopping diarrhoica, clearing lung and eliminating phlegm, eliminating carbuncle and alleviating edema; the composition is a traditional Chinese medicine, is green, has no medicine residue, is safe and reliable and is one of main development directions of medicine for preventing and treating diseases in the fox raccoon aquaculture industry in future.

The invention provides preparation and application of premixed feed for a raccoon dog in a growing period, belongs to the technical field of feed, and particularly relates to premixed feed and a preparation method for increasing feed utilization rate and the growth speed and immunity of the raccoon dog in the growing period. According to the premixed feed, the digestive rate of the raccoon dog in the growing period for nutrient substances in the feed is increased, the intestinal environment of the raccoon dog in the growing period can be improved, intestinal immunity is improved, the feed utilization rate is increased, reduction of the growth performance due to diarrhea of the raccoon dog in the growing period can be prevented, the growth speed and immunity of the raccoon dog in the growing period can be effectively improved, intestinal diseases such as the diarrhea of the raccoon dog in the growing period is reduced, the anti-virus capability and immunity of animals are enhanced, the economic benefit of artificial raccoon dog breeding is improved, and drug residues and heavy metal content in a carcass are reduced.

The invention discloses maker polypeptides and a method for detecting non-edible meat from edible meat. The maker polypeptides comprise any one group, any two groups or all three groups of maker polypeptides from the following groups (a) to (c): the group (a) is maker polypeptides specific to foxes, raccoon dogs and minks, and comprises polypeptides shown as SEQ ID NO.1; the group (b) is maker polypeptides specific to foxes and raccoon dogs, and comprises polypeptides shown as SEQ ID NO.2 and / or 3 and / or 4 and / or 5; and the group (c) is maker polypeptides specific to minks, and comprises polypeptides shown as SEQ ID NO.6 and / or 7 and / or 8 and / or 9 and / or 10. According to the maker polypeptides and the method, a technology for identifying non-edible meat in different edible meat from a polypeptide level is established, and a new effective means is provided for improving food safety.

The invention discloses a raccoon dog parvovirus virus-like particle, a preparation method thereof and application thereof as well as a vaccine prepared by the virus-like particle, and belongs to thefield of recombinant vaccine preparation. In order to solve the problem that raccoon dog parvovirus enteritis inactivated vaccine is relatively low in immunity and virulence of the live vaccine is strong, the invention provides the raccoon dog parvovirus virus-like particle. A raccoon dog parvovirus gene RDPV-VP2-OPTI optimized through a codon is connected with an insect expression vector pFastBac1 to obtain a donor plasmid; the donor plasmid is converted into baculovirus DH10 Bac E.coli to culture to obtain recombinant baculovirus for expressing raccoon dog parvovirus RDPV-VP2 protein, is transfected to an SF21 cell, and is cultured until the cell is infected to collect a cell virus solution. After the raccoon dog parvovirus virus-like particle is mixed with an aluminum hydroxide gel adjuvant, the raccoon dog parvovirus enteritis vaccine can be obtained. The raccoon dog parvovirus virus-like particle is suitable for preparing the raccoon dog parvovirus enteritis vaccine.