70 results about "Reproductive technology" patented technology

The present invention relates to the use of an oxytocin receptor antagonist in females undergoing embryo transfer as part of an assisted reproductive technology. In particular, methods are provided for increasing ongoing implantation rate, increasing ongoing pregnancy rate, increasing clinical pregnancy rate, and/or increasing live birth rate in a female subject undergoing embryo transfer. Specifically, the antagonists are released in the luteal phase when the endometrium is receptive for embryo implantation and/or when the embryo has reached the blastocyst-stage.

Artificial insemination of female animals and in vitro fertilization of oocytes with fresh or frozen-thawed semen have been applied to the reproduction of animals. According to the traditional theory and conventional procedures, a great number of sperm cells are needed to ensure a successful fertilization.

The invention relates to the field of genetic diagnosis and human assisted reproduction, and more particularly relates to an embryo pre-implantation genetic diagnosis technique (PGD). According to the method provided by the invention, for patients suffering from chromosomal balanced translocation (including reciprocal translocation and Robertson translocation) and mates thereof, family haplotype linkage analysis is performed on the chromosomes of the embryos obtained by in vitro fertilization and the relatives of the translocation carrier, the embryos with chromosomal balanced translocation and the embryos with normal chromosomes can be rapidly, simply and accurately distinguished, and at the same time, the chromosome copy number variation of the embryo is screened, so that the clinical pregnancy rate is improved, the genetic transmission of the chromosomal balanced translocation to the next generation is timely blocked before embryo implantation, and thus the development and progress of the human assisted reproductive technology are promoted to a certain extent.

The invention discloses in vitro human sperm culture solution for improving sperm motility and application thereof. The culture solution contains fructose 6-phosphoric acid (F6P). The in vitro human sperm culture solution for improving the sperm motility of human sperms is used for culturing the purified human sperms which leave from a human body; the vitality of normal human sperms is capable of maintaining sperm movement and can be obviously improved; and a meaningful reference is provided for improving and assisting the reproductive technology. The F6P is directly used for cultivating and diluting the culture solution, and does not relate to organic solvents such as ethanol or dimethylsulfoxide (DMSO) and the like; the toxic action of the ethanol or the DMSO on the sperms is avoided; by adopting the F6P added to the sperm culture solution, the vitality of the sperms of clinical patients with asthenospermia can be obviously improved; the improvement degree of the vitality is the most obvious for severe oligospermia; the activity of a part of cultured sperms reaches the normal sperm vitality range; and the improvement degree of the vitality for the severe oligospermia is the most obvious. This fully demonstrates that low motility of the sperms of the patients with asthenospermia can be improved by using of the F6P.

The invention relates to a sperm washing liquid and a preparation method thereof and belongs to the technical field of human assisted reproduction. The sperm washing liquid is prepared by dissolving heparin sodium dry powder or heparin sodium salt solution in a salt solution containing antibiotics and indicator. Heparin sodium is added into the sperm washing liquid, and by combination with a receptor on the surface of sperm, heparin can activate a signal transduction system coupled with the receptor, so a capacity function of sperm mitochondria and calcium ion internal flow are improved mildly and effectively, sperm activity is improved, and not only can the artificial insemination success rate be improved, but also safety of the assisted reproductive technology is strongly ensured.

The invention relates to sperm motility fluid and a preparation method thereof, belonging to the technical field of human assisted reproduction. The sperm motility fluid is prepared by dissolving ATPsodium salt, dried heparin sodium powder/heparin sodium salt solution into a salt solution containing antibiotics and indicators. The ATP sodium salt and the heparin sodium are added into the sperm motility fluid disclosed by the invention, the ATP sodium salt can provide energy substances for sperm motility and contribute to sperm middle-piece mitochondria capacity, the heparin can activate a receptor-coupled signal transduction system by binding with a receptor on the sperm surface, the capacity function and calcium influx of the sperm mitochondria are gently and effectively improved, and the sperm motility is further improved. Therefore, the sperm motility can be effectively improved, the capacity of the sperm middle-piece mitochondria is wakened and activated, and the success rate of intra-uterine insemination (IUI) is improved. Moreover, a powerful guarantee is provided for the safety of an assisted reproductive technology.

The invention relates to application of n-octanol to the prolonging of the in-vitro ageing of an ovum of a mammal. According to the application, the n-octanol is added in a culture solution containing fetal calf serum, and the concentration of the n-octanol is 0.1-4 mM. By using the application, the speed of the in-vitro ageing of an oocyte of the mammal is obviously deferred; meanwhile, the in-vitro development capability of the oocyte is guaranteed, so as to provide a novel approach for deferring or reversing the ageing of the ovum; and a reference is provided for improving an auxiliary reproductive technology. The technology provided by the invention is not reported in public at home and abroad, has extremely high innovativeness and has a great impellent action on the field of the embryo engineering of the mammal.

The invention relates to methods for assisted reproduction technology in mammals. Specifically the invention relates to methods for in vitro mammalian oocyte culture and oocyte maturation, in vitro fertilization, and in vitro embryo development.

The invention belongs to the technical field of assisted reproduction, and relates to an oocyte activating solution and application thereof. The oocyte activating solution comprises 1.1 to 1.8 mg/L ofionomycin, 6 to 12 mg/L of calcium ion carrier A23187, 63 to 94 mug/L of puromycin, 19 to 43 mg/L of 6DMAP, 3.4 to 6.6 mg/L of actinone CHX, 28 to 53 mug/L of phorbol PMA, 7.8 to 12.7 mug/L of inositol triphosphate, 1.5 to 2.4 g/L of SrCl2, 16 to 25 mg/L of heparin, and basic culture medium. The oocyte activating solution can effectively activate oocytes, and is suitable for activating the oocytes after ICSI in an in-vitro fertilization assisted reproduction technology.

The invention relates to the technical field of assisted reproduction, and discloses a cryoprotectant and an application thereof, a sperm refrigerating fluid and a preparation method of the sperm refrigerating fluid. The sperm refrigerating fluid comprises a cryoprotectant, a composition and a basic culture solution, wherein the cryoprotectant comprises 0.3 to 1.1 mg/L of paclitaxel, 16 to 22 g/Lof trehalose, 7 to 13 g/L of cane sugar and 80 to 250 mL/L of glycerinum; the composition comprises 12 to 26 mg/L of resveratrol, 0.2 to 1.9 mg/L of reduced glutathione, 436 to 775 mg/L of lecithin, 0.5 to 1.6 mg/L of cholesterol, 9 to 17 mg/L of ATP sodium salt, 11 to 28 mg/L of heparin, 93 to 299 mg/L of phosphatidylserine and 5 to 25 mg/L of coenzyme Q10. The sperm refrigerating fluid can reduce damage of sperms in low-temperature freezing and freezing resuscitation processes, improve vitality of the sperms after freezing resuscitation, prolong survival time of the sperms after freezing resuscitation, promote sperm capacitation and enhance penetrating fertilization capacity of the sperms to egg cells. Thus, the fertilization success rate and blastocyst formation rate of the ova are improved, and finally the effect of improving the pregnancy success rate of the in-vitro fertilization assisted reproduction technology is achieved.

The invention relates to a cleavage culture solution and a preparation method thereof and belongs to the technical field of human assisted reproductive technology. The cleavage culture solution has the advantages that the cleavage culture solution is prepared by adding alanine, aspartic acid, asparagine, glutamic acid, glutamine, glycine, proline, taurine, serine, threonine, tyrosine, methionine,lipoic acid, hyaluronic acid and a chelating agent into a salt solution, the activity and nutrition of an embryo can be kept effectively, the chelating agent can chelate some unnecessary heavy metal ions, the toxicity of the culture solution is lowered, the development of the early embryo can be promoted favorably, the success rate of the assisted reproductive technology is increased, and powerfulguarantee is provided for the safety of the assisted reproductive technology.

The invention relates to a strengthening agent for reproductive capacity of foxes and raccoon dogs, which belongs to the field of animal nutrition and reproductive technology.Every 100g product consists of 20-30g of soybean flour, 15-20g of fish powder, 8-12g of algae powder, 10-15g of yolk powder, 3-8g of Chinese onion seed powder, 2-6g of Chinese chive seed powder, 0.8-1.5g of linoleic acid, 100-500mg of vitamin E, 100-200mg of methionine manganese, 300-800mg of methionine zinc, 50-100mg of lysine selenium and 1.5-4.0g of vitamin complex , the rest is corn-meal. The invention can supply the foxes and raccoon dogs with sufficient nutrition, improve development of reproductive organs of the foxes and raccoon dogs, promote secretion of androgen, increase sperm motility, survival rate and fertilization rate, reduce sperm abnormality rate and enhance reproductive capacity of male foxes and raccoon dogs.

A method for adjusting the maturation state of mammalian sperm for use in assisted reproductive technologies (ART) is disclosed. A mammalian ejaculate is provided and incubated under controlled conditions. Aliquots of the ejaculate are assayed during incubation period at intervals to determine maturation state and changes in the maturation state by observing the percent positive cells in the aliquot. The assays are repeated with successive aliquots at intervals during incubation to observe real time changes in the maturation state. The ejaculate remaining is processed for the desired ART after the percentage of positive cells in the latest aliquot being assayed begins to decline.

The invention belongs to the field of heredity diagnosis and mankind assisted reproduction, and more particularly, relates to preimplantation genetic testing (PGT). According to the method of the invention, pedigree haplotype linkage analysis is performed on a patient with chromosome inversion, a spouse thereof, an embryo after external fertilization, and the chromosome of an inversion carrier relative, thereby quickly, simply and accurately discriminating the chromosome inversion carrying type embryo and the chromosome normal type embryo. The chromosome normal type embryo is preferably transplanted. The method realizes heredity transmission blocking of chromosome inversion to a next generation before embryo transplantation in time. The method has an important meaning for promoting sound child rearing of population, reducing birth defects and improving mankind reproduction health. Furthermore the method promotes development of mankind assisted reproduction technology to a certain extent.

The invention belongs to the field of animal breeding and particularly relates to an artificial reproduction-hybridization joint breeding method for a meat goat. The method comprises the steps of selecting one goat variety with good meat performance as a female parent, selecting another goat variety with a large body size as a male parent; culturing oocytes of the female parent in vitro into mature oocytes containing a first polar body, and soaking semen of the male parent with a semen motility enhancement liquid and then preparing a semen suspension; carrying out in vitro fertilization on themature oocytes and the semen suspension, culturing zygotes into a hybrid blastocyst form, transplanting hybrid blastocysts into a body of the female parent for developing to natural delivery in the body of the female parent and obtaining hybrid F1; and backcrossing the hybrid F1 and the female parent, backcrossing backcross descendants and the female parent for multiple times until a breeding objective is met, and carrying out crossbred fixing to obtain a new variety/line with good characters. According to the method, an artificial assistant reproduction technology is combined with a traditional hybridization technology, so that the number of animal embryos can be increased, the survival rate of the animal embryos can be improved and the method has a good application prospect in the fieldof animal breeding.

The invention relates to a blastocyst culture liquid and a preparation method thereof, and belongs to the field of human-assisted reproduction technique. The blastocyst culture liquid is prepared by adding alanine, aspartic acid, glutamic acid, glycine, proline, taurine, serine, threonine, tyrosine, methionine, lipoic acid, arginine, cystine, histidine, isoleucine, leucine, lysine, phenylalanine,threonine, valine, folic acid, inositol, vitamin B12, vitamin B6, vitamin B5, vitamin B2, vitamin B1, vitamin B2, thiamine, hypoxanthine, pantothenic acid and chelating agent into a salt solution. Theblastocyst culture liquid has the advantages that the energy and nutrition of embryo are effectively maintained; several unnecessary heavy metal ions can be chelated by the chelating agent, so that the toxicity of the culture liquid is decreased; the blastocyst culture liquid is suitable for promoting the development of embryo and the formation of blastocyst in the early phase, improving the success rate of assisted reproduction technique, and providing powerful guarantee for the safety of assisted reproduction technique.