26 results about "Reproductive technology" patented technology

The invention provides a method for overspeed preparation of gene edited goldfish. The method comprises the following steps: S1, obtaining gobiocypris rarus without primordial germ cells as receptor fish; s2, designing a target spot for the goldfish amh gene, introducing a Cas9 / gRNA complex into a goldfish fertilized egg, performing sequencing verification to obtain a mutant individual, and feeding the mutant individual as a donor fish; s3, taking gonads of donor fish, cutting into pieces, performing enzymolysis digestion, filtering, and performing density gradient centrifugation to enrich donor germline stem cells; s4, transplanting the germline stem cells of the donor fish into the receptor fish body, and feeding to obtain the gene edited goldfish sperms and ova. S5, hybridizing and feeding goldfish amh mutant sperms and ova produced by gobiocypris rarus to obtain gene edited goldfish individuals. According to the invention, gobiocypris gobiocypris is used as a receptor, the cross-subfamily gene edited goldfish is used as a donor, and an efficient, stable and easy-to-popularize method for preparing the gene edited goldfish is provided by utilizing a belly borrowing reproduction technology.

The invention discloses application of Kitl in promoting meiosis of germ cells cultured in vitro. By adding a small molecule Kit ligand (Kitl), germ cells (including primordial germ cells PGCs, primordial germ cell-like cells PGCLCs and the like) cultured in vitro are promoted to enter and complete the first meiosis earlier stage, meiosis key protein expression is improved, and homologous chromosome association and recombination efficiency is improved. The method has a wide transformation medical prospect in the fields of human assisted reproductive technology and reproductive medicine.

A novel PRP manufacturing set comprises a blood drawing tube, a PRP sampling tube and a common syringe, the outer wall of the blood drawing tube is fixedly connected with a 40ml scale line, the outer wall of the PRP sampling tube is fixedly connected with a 20ml scale line, and the front end of the blood drawing tube is provided with a butt joint tube structure. According to the novel PRP manufacturing set, the first piston head at the front end can be pushed through the detachable push rod to be pulled and slide in the blood drawing tube, the first piston head can drive the interior of the blood drawing tube to achieve blood drawing, blood feeding and PRP manufacturing through being communicated with the PRP sampling tube through negative pressure, the PRP sampling tube is designed to be of a short and thick structure, the maximum capacity is 20 ml, the short and thick design is adopted, the head can be clamped, and the PRP sampling tube is convenient to use. According to the novel PRP manufacturing set, blood drawing operation does not need to be completed, blood can be quantitatively conveyed into the PRP sampling tube through a long injector structure and a short injector structure, the PRP manufacturing process can be more accurately and reasonably achieved, and the using effect of the novel PRP manufacturing set is improved.

The invention belongs to the technical field of biology, and particularly relates to a method for improving in-vitro mature oocyte telomere length and maturation quality and application of the in-vitro mature oocyte telomere length and the maturation quality. And daphnetin. According to the preparation disclosed by the invention, at least one of the Sema3 type protein and the daphnetin is added, so that the telomere length of an oocyte and an in-vitro embryo is effectively maintained, repaired or prolonged, the maturation quality of the in-vitro oocyte and the development potential and development efficiency of the in-vitro embryo are further remarkably improved, and further, the development potential and development efficiency of the in-vitro embryo are further improved. The application provides a more effective new strategy for in-vitro embryo production of livestock, animal genetic improvement, assisted reproduction technology and the like, and the application prospect is wide.

Stabilized amorphous calcium carbonate (ACC) for treatment of several neurological, muscular and infertility diseases and conditions is provided. In particular, the stabilized ACC may be used in the treatment of axonal defects and muscular dystrophy. In addition, provided are improved methods used in assistant reproductive technology. Examples of such methods are in vitro fertilization and improvement of sperm quality. The improved IVF method, for example, comprises addition of the stabilized ACC to the cell culture medium in which the stages of fertilization and embryo development occurs.

The invention relates to a low-temperature preservation method for sheep semen, and belongs to the technical field of animal reproduction. The invention provides a method for low-temperature preservation of sheep semen, which comprises the following steps: (1) collecting breeding male sheep semen by a false vagina method to obtain the collected sheep semen; (2) mixing the sheep semen collected in the step (1) with a semen diluent and fullerene to obtain a sheep semen diluent; and (3) preserving the sheep semen diluent under the condition of 2-8 DEG C. The sheep semen is preserved according to the method, the effective application time of sperm preservation can be remarkably prolonged, the blastocyst forming rate of in-vitro fertilization embryos can be remarkably increased, and the method is applied to reproduction technologies such as artificial insemination (AI) and in-vitro fertilization (IVF) of sheep, the utilization rate of high-quality breeding rams can be increased, and genetic resource protection and efficient breeding can be promoted.

The present discloses provides the use of tributyrin as an additive for embryonic development culture medium in vitro, in the present disclosure, the tributyrin is applied as an additive to the development culture medium of mouse embryos in vitro for the first time, the tributyrin can significantly increase the rate of blastocyst, and reduce the ROS content in the embryos, improve the mitochondrial membrane potential in the embryos, increase the ATP level and the expression of antioxidant genes in the embryos, improve the DNA methylation and histone modification level in the embryos, promote the embryos development in vitro; In addition, tributyrin, as natural antioxidant and apparent drug, which is safe, non-toxic and side effects; the tributyrin provides strong support for the efficient embryos development of human assisted reproductive technology, mammalian fertilization embryos, parthenogenetic embryos and somatic cell cloned embryos and other embryo engineering technologies in vitro.

This invention discloses a method for cryopreservation of donkey semen using antioxidants, belonging to the field of animal reproductive technology. The method is based on a specific basal diluent, with the addition of targeted mitochondrial antioxidants SKQ1 or SS-31. First, a basal diluent containing sugars, citrate, proline, skim milk powder, and antibiotics is prepared. Then, SKQ1 (dissolved in DMSO) or SS-31 (dissolved in physiological saline) is separately prepared into a stock solution, which is then mixed with the basal diluent to obtain a composite preservation solution. Finally, the donkey semen treated with this composite preservation solution is diluted and stored at 4°C. The method of this invention can significantly improve sperm motility and acrosome integrity rate of donkey semen stored at 4°C for 5 days, reduce ROS content and abnormality rate, and significantly increase the insemination and conception rate of the preserved semen to over 40%, effectively extending the practical preservation period of donkey semen.

This invention discloses a novel use of pyrroloquinoline quinone (PQQ) in improving conception rates after artificial insemination in beef cows, as well as compositions containing rumen-protected PQQ and their application methods, belonging to the field of animal nutrition and reproductive technology. Addressing the problem of low conception rates in beef cattle after artificial insemination due to early embryonic oxidative stress death, this invention proposes continuously adding an effective dose of rumen-protected PQQ to the cow's diet starting from the day of artificial insemination. This method significantly increases the conception rate of cows from approximately 40.00% in the control group to 80.00%. This invention is safe, effective, and easily applicable in large-scale farming.

The invention provides a myxocyprinus asiaticus individual genetic resurgence method which comprises the following steps: S1, taking a myxocyprinus asiaticus gonad tissue, cleaning, cutting into pieces, adding an L-15 culture medium containing double antibodies and a DPBS buffer solution, and centrifuging to obtain the gonad tissue; s2, performing water bath on the gonad tissues to obtain resuscitated gonad tissues; s3, digesting, filtering and centrifugally purifying the resuscitated gonad tissues to obtain germline stem cells; s4, injecting morpholino to obtain gobiocypris rarus without primordial germ cells; and S5, transplanting the germline stem cells into gobiocypris rarus juvenile fish bodies, and culturing to obtain the myxocyprinus asiaticus gametes. S6, the generated myxocyprinus asiaticus gametes are fertilized, and myxocyprinus asiaticus individuals are obtained. According to the method, the sperms of the myxocyprinus asiaticus are successfully obtained by utilizing a gonad cryopreservation and abdomen borrowing reproduction technology, and a feasible way is provided for individual genetic resurgence of rare and endangered fishes.

The utility model relates to the field of assisted reproductive technology equipment discloses a kind of bovine in vitro embryo culture environment simulation device, including device body assembly, the device body assembly includes simulation device body, the outer surface of the simulation device body is rotatably connected with turnover observation window, the bottom of the simulation device body is fixedly connected with buffer rod by bottom mounting seat.The utility model is through the series connection effect of first spring damper, buffer spring and second spring damper, device forms gradient shock-absorbing network in three-dimensional space, horizontal direction impact is preferentially absorbed by first spring damper and converted into heat energy dissipation, vertical load is guided to spiral spring by buffer rod and carried out flexible support, extend the action cycle to reduce peak acceleration, moving block linkage mechanism cooperates second damper to realize the vector decomposition of oblique disturbance, finally reduce the vibration amplitude at culture dish, provide nearly stationary growth interface for embryo cell division.

Disclosed in the present invention are a composition and method for increasing the number and proportion of inner cell mass cells of an in vitro embryo and use. According to the present invention, in view of the problems of in vitro fertilized embryos such as low blastocyst rate during early development, low inner cell mass cell number in the blastocyst stage, low proportion of the inner cell mass cells, and poor subsequent embryonic development potential, by adding one or two of regulators folic acid and laxiflorin B into the culture medium for early embryo in vitro culturing, the blastocyst rate, the number and proportion of the inner cell mass cells in the blastocyst, the survival rate of blastocysts after extended culturing, and the diameter of blastocysts are significantly improved, effectively improving the developmental potential of in vitro embryos. In addition, the present invention also effectively improves the ratio of in vitro fertilized embryos that can be subsequently transplanted. The present invention is low in cost, safe, and efficient, provides a new strategy for in vitro embryo production of livestock and development of human-assisted reproductive technology, and exhibits broad application prospects.

The present invention relates to assisted reproductive management technologies common to animal husbandry, more specifically technologies and processes enabling timed artificial insemination (TAI) and timed embryo transfer (TET), superovulation for multiple ovulation embryo transfer (MOET) and superstimulation for ovum pickup (OPU) prior to in-vitro fertilization. More specifically, the present invention is directed to simplified, single-injection methods and formulations to control the onset of estrus and ovulation occurring within the reproductive technologies described above. The formulations focus on progesterone (P4) and luteinizing hormone (LH).

The invention provides a trophoblast cell feature extraction method and device, electronic equipment and a storage medium, and relates to the technical field of assisted reproduction. The method comprises the following steps: determining a mask image of an inner cell mass in a target blastocyst, a first trophoblast cell mask image and a second trophoblast cell mask image; according to the first trophoblast cell mask image, the second trophoblast cell mask image and the mask image of the inner cell cluster, determining marking information of trophoblast cells in the target blastocyst; performing curved surface mapping on the mask image of the inner cell cluster, the mask image of the first trophoblast cell and the mask image of the second trophoblast cell according to marking information of the trophoblast cells to generate a curved surface mapping result; and according to a curved surface mapping result, carrying out feature extraction on the trophoblast cell region in the target blastocyst to obtain multiple three-dimensional features of the trophoblast cell region. According to the method, more core three-dimensional characteristics of trophoblast cells can be fully excavated, and the blastocyst development potential evaluation accuracy is improved.

This invention provides a method and system for predicting the potential of bovine in vitro embryo production based on AMH concentration, relating to the field of livestock reproductive technology. The method includes: obtaining a blood sample from a donor cow before the start of the in vitro embryo production cycle; performing serum separation processing on the blood sample to obtain a serum sample; detecting the anti-Müllerian hormone concentration in the serum sample to obtain a serum AMH concentration value; and predicting the number of follicles in the donor cow based on the serum AMH concentration value using a cubic polynomial regression model. This invention establishes serum AMH concentration as an objective biomarker for predicting bovine in vitro embryo production potential and constructs a corresponding quantitative prediction model, achieving early, non-invasive, and quantitative prediction of the individual potential of donor cows before incurring high production costs.

The invention discloses a library building method for detecting G-quadruplex structures in oocytes, early embryos and trace cells, and belongs to the technical field of whole genome immunoprecipitation library building. The DNA G-quadruplex structure provided by the invention is a DNA secondary structure which is different from a classic double-helix structure; g-quadruplex structure abnormity can damage telomere stability, genome stability, transcriptional activity and the like; this is an important reason for poor ovum quality and embryonic development retardation. The method provided by the invention can be used for evaluating the quality of the ova of the infertile women and judging reasons causing immaturity of the ova; abnormal enrichment of in-vivo and in-vitro development arrest embryo whole genome G-quadruplex is detected and can be used as an important index for screening reasons causing embryonic development retardation; the method can be used for guiding whether corresponding clinical patients are suitable for a series of assisted reproductive technologies (ART) such as in vitro fertilization-embryo transfer (IVF-ET) and the like.

The application provides a preparation for enhancing the in-vitro proliferation capacity of pig spermatogonial stem cells, and belongs to the technical field of livestock reproduction. The preparation is composed of a basic culture solution, basic maintenance components, supportive growth factors and a core active component polypeptide Japonicin-1Npb. Research shows that under the condition of approaching the physiological temperature (38.5 DEG C) of pigs, the addition of 25-100 mu g / mL of the polypeptide Japonicin-1Npb can significantly improve the EdU positive rate of pig spermatogonial stem cells, promote cell proliferation, specifically up-regulate the expression of stemness genes PLZF, UCHL1 and GFR alpha 1, and inhibit the differentiation gene c-KIT. The application solves the problems of low in-vitro expansion efficiency and easy loss of stemness of pig spermatogonial stem cells, and the cells still retain good meiotic differentiation potential after the removal of the preparation, thereby providing technical support for the development of high-quality genetic resources of pigs and the research of reproductive biology.

The invention provides a method for reconstructing functional gametes of fish cryopreserved germline stem cells based on zebra fish nanos2- / -mutant receptors, which specifically comprises the following steps: S1, collecting embryos of selfing progenies of zebra fish nanos2- / -and nanos + / -mutants, culturing and screening out nanos2- / -mutants to obtain receptor fish; s2, after gonads of the cryopreserved fishes are recovered, donor fish germline stem cells are obtained; s3, transplanting the germline stem cells of the body fish in the step S2 into the receptor fish in the step S1, culturing to obtain positive individuals, and continuously culturing to obtain the functional gametes of the zebra fish. Based on a selection strategy taking a nanos2 <- / -> mutant receptor as an excellent receptor, verification is carried out in experimental animal zebra fish cryopreserved germline stem cells: colonization and differentiation of germline stem cells from a donor source can be effectively supported, so that functional ova and sperms are generated, and the limitation that donor ova are difficult to obtain in an existing belly borrowing reproduction technology is broken through; and a new way is provided for long-term preservation and recovery of fish germplasm resources.

This application provides a method and related equipment for DNA integrity assessment based on single sperm Raman spectroscopy, belonging to the field of sperm quality assessment technology in assisted reproductive technology. This method, based on a machine learning algorithm, directly assesses DNA integrity using the Raman spectra of single sperm, thereby achieving non-destructive screening of individual high-quality sperm in assisted reproductive technology. The process of constructing the algorithm training sample dataset includes a step-by-step fixation and analysis method for sample single sperm: first, Raman spectroscopy testing is performed, followed by fluorescence staining analysis, reducing the impact of sperm position changes during detection, analysis, or staining. Then, optical and fluorescence images of single sperm within preset shape units are matched to determine the validity of the sample single sperm. Finally, a training sample dataset is constructed based on the relevant data of valid single sperm, establishing a correlation between the Raman spectrum of the same single sperm and its sperm chromatin structure analysis results.

The application discloses an ultrasonic imaging method based on image fusion, and belongs to the field of assisted reproductive technology.The method comprises the following steps: collecting image data of a uterus by using uterine cavity ultrasonic and abdominal ultrasonic; preprocessing the collected uterine cavity ultrasonic image and abdominal ultrasonic image; extracting features and matching features of the two preprocessed images; transforming and aligning the two images after feature extraction and feature matching; and distributing weight of the two aligned images according to a fusion weight, and fusing the two images into a comprehensive image.The method combines the high-resolution characteristics of uterine cavity ultrasonic with the wide field of view of abdominal ultrasonic, and provides more comprehensive anatomical information by fusing the two ultrasonic images, overcoming the limitations of single imaging technology. The method provides clearer and more comprehensive ultrasonic imaging, improves the definition of the abdominal ultrasonic image, and makes the evaluation of the endometrium and embryo implantation site more accurate.

The utility model provides a kind of integrated frozen carrier, belong to the field of assisted reproductive technology. Including carrier rod body and sheath tube. Among them, one end of carrier rod body is provided with carrier sheet and is coaxially sliding fit with sheath tube. The one end of sheath tube is coaxially connected with protective sleeve, the end of protective sleeve away from sheath tube is provided with sealing metal ring, guide groove for guiding carrier sheet is opened on sheath tube lateral wall along axial direction, guide groove is communicated with protective sleeve, carrier sheet can be moved between the retraction position in protective sleeve and the advancing position outside protective sleeve, sealing metal ring is configured to make the end of protective sleeve closed when being radially compressed. The integrated frozen carrier can reduce the difficulty of sample vitrification freezing and storage operation using frozen carrier, improve operation efficiency while reducing operation risk.

The utility model discloses a split type vitrification freezing carrying rod, which belongs to the technical field of assisted reproduction, and comprises a carrying piece for placing a sample body and a carrying rod connected with the carrying piece, the tail end of the carrying rod is a handheld part, one end of a connecting part is connected with a thermal plugging head, the other end of the connecting part is matched with the tail end of the handheld part through a clamping assembly, and the carrying piece is connected with the carrying rod. The connecting part is inserted into the sheath after being matched with the handheld part through the clamping assembly, the handheld part is provided with a grip, the grip extends to form a stroke rod, the end of the stroke rod is fixedly connected with the protruding block, a sliding block is arranged on the stroke rod, the clamping assembly comprises a connecting frame with an inner cavity, a penetrating groove is formed in the side face of the connecting frame, a sliding rod is arranged in the penetrating groove, and a spring is arranged on the sliding rod. The technical problem that an integrally-injection-molded lengthened carrying rod cannot be detached is solved, and the loading rod is mainly applied to the aspect of cryopreservation of ova, embryos and cystic fetuses.

The application discloses an embryo development stage positioning method and device and a medium, and relates to the technical field of assisted reproduction, and comprises the following steps: acquiring a frame sequence of an original embryo development video, and constructing a positive sample pair and a negative sample pair; adopting a contrast loss function to optimize parameters of a feature encoder; calculating an inter-frame similarity matrix by using the optimized feature encoder; calculating variances in different time windows based on the inter-frame similarity matrix, dividing a similarity region by taking multiple optimal change points detected on a time axis by dynamic programming as time sequence boundaries, setting different optimal change point quantity sets, and generating multi-scale time sequence nominations; extracting video-level features from the original embryo development video; cutting nomination-level features from the video-level features according to the time sequence nominations; inputting the video-level features and the nomination-level features into a neural network after splicing, and outputting a development stage category and a boundary regression result of each time sequence nomination; and the method improves the accurate positioning capability of the time sequence boundary region.

This utility model relates to the field of assisted reproductive technology, specifically to an embryo transfer tube, comprising a first tube segment and a second tube segment connected to each other. The inner diameter of the second tube segment is larger than that of the first tube segment. A piece of absorbent cotton is placed inside the second tube segment. An annular limiting seat is provided on the inner wall of the second tube segment at the end away from the first tube segment, and the inner diameter of the annular limiting seat is smaller than that of the second tube segment. By setting an annular limiting seat at the end of the larger-diameter second tube segment, this utility model can reduce the air intake cross-sectional area, increase airflow resistance, and make the negative pressure generated by the suction bulb more gradual. This facilitates the operator's precise control of the embryo aspiration position, thereby reducing the risk of embryos being accidentally aspirated into the second tube segment. Simultaneously, the absorbent cotton placed inside the second tube segment further intercepts any embryos and fluids that may be accidentally aspirated.

The application discloses a blastocyst formation prediction method, system and device based on embryo formation score, relates to the field of assisted reproductive technology, and comprises the following steps: collecting embryo morphological information of a patient, and constructing a training set and a verification set; based on the training set, a generalized estimating equation is used for modeling to obtain a blastocyst formation prediction model; the blastocyst formation prediction model is verified and calibrated based on the verification set; and whether the blastocyst is formed is predicted through the calibrated blastocyst formation prediction model. The application can quantitatively generate the blastocyst formation opportunity of a single embryo and a patient individual, and provides a basis for clinical decision-making.

The present disclosure discloses a composition, method and application thereof for improving the quality of in vitro maturation of oocytes and the pregnancy rate after in vitro produced embryos transfer. The present disclosure aims at the problems of low maturation rate and poor subsequent embryo development potential caused by metabolic disorders during in vitro maturation of oocytes, and provides a composition including two or more regulators in vitamin C, L-carnitine and thrombopoietin to regulate the metabolism of oocytes, thereby greatly improving the in vitro maturation rate of oocytes and the subsequent embryo development potential. The present disclosure not only improves the efficiency of in vitro embryo production of livestock, but also provides a reference for improving the efficiency of human assisted reproductive technology. The method is simple, safe and efficient, and has great application value and broad application prospects.