15 results about "Atp level" patented technology

The present disclosure provides polypeptide constructs that, when exposed to sufficient levels of ATP, cause them to assemble into dimers or higher order complexes (e.g., trimers, tetramers, etc.), thereby acting as immune cell function agonists. The constructs can also be conjugated to one or more therapeutic, chemotherapeutic, or labeling agents. The complexes of the constructs are capable of stimulating immune cells (e.g., cytotoxic CD8+ T cells and / or NK cells) that function to promote an anti-tumor immune response, and delivering the conjugated agent to the tumor microenvironment. The constructs can be used as anti-cancer / therapeutic agents for the treatment of solid tumors with elevated ATP levels.

The present discloses provides the use of tributyrin as an additive for embryonic development culture medium in vitro, in the present disclosure, the tributyrin is applied as an additive to the development culture medium of mouse embryos in vitro for the first time, the tributyrin can significantly increase the rate of blastocyst, and reduce the ROS content in the embryos, improve the mitochondrial membrane potential in the embryos, increase the ATP level and the expression of antioxidant genes in the embryos, improve the DNA methylation and histone modification level in the embryos, promote the embryos development in vitro; In addition, tributyrin, as natural antioxidant and apparent drug, which is safe, non-toxic and side effects; the tributyrin provides strong support for the efficient embryos development of human assisted reproductive technology, mammalian fertilization embryos, parthenogenetic embryos and somatic cell cloned embryos and other embryo engineering technologies in vitro.

This invention discloses the application of disodium 4,4'-diisothiocyanate-2,2'-stilbene sulfonate in the preparation of drugs for the prevention, delay, and / or treatment of blue light-induced retinal damage. It belongs to the field of biomedical technology. This invention demonstrates for the first time that sodium DIDS can effectively protect photoreceptor cells from blue light damage: at the cellular level, sodium DIDS can restore mitochondrial membrane potential, increase ATP levels, reduce reactive oxygen species accumulation, and inhibit mitochondrial-dependent apoptosis; at the animal level, intravitreal injection of sodium DIDS can improve vision in mice exposed to blue light, restore the amplitude of a-waves and / or b-waves in electroretinograms, and protect the outer segment length and outer nuclear layer thickness of cone cells. Sodium DIDS provides a novel therapeutic strategy for blue light-induced retinal photodamage through multi-target synergistic protection of mitochondrial function.

The invention discloses a multi-mode mitochondrial activation device, wearable infrared physiotherapy equipment and application. The multi-mode mitochondrial activation device comprises a shell; the multi-mode stimulation unit is arranged on the shell and comprises at least one stimulation source of a light biological regulation module for providing light stimulation, a vibration module for providing vibration stimulation and an electrical stimulation module for providing electrical stimulation, and a temperature and light intensity sensing module; the control and communication module is integrated on the shell and comprises a micro-control unit and a wireless communication unit, and the micro-control unit is electrically connected with the multi-mode stimulation unit and is configured to receive feedback data of the temperature and light intensity sensing module and dynamically control at least two of the light biological regulation module, the vibration module and the electrical stimulation module; and the power supply module is electrically connected with the multi-mode function unit and the control and communication module. The method has the beneficial effects that after multi-modal stimulation, the ATP level of cells is increased, ROS is reduced, the mitochondrial membrane potential is recovered, and the tissue energy metabolism is remarkably improved.

The invention discloses a cyclometalated iridium (III) complex for targeting glycometabolism and a synthetic method and application thereof.The cyclometalated iridium (III) complex for targeting glycometabolism has good cell uptake capacity and capacity of targeting mitochondria in tumor cells, can inhibit PDK2 expression level in the tumor cells, meanwhile, reduces the pPDH / PDH ratio, and has the advantages of being capable of inhibiting tumor cell proliferation and inhibiting tumor cell proliferation. Therefore, the glycolytic pathway of tumor cells is inhibited; besides, the complex disclosed by the invention also can cause mitochondrial membrane potential reduction and intracellular ATP level reduction by inducing generation of active oxygen, so that tumor cell apoptosis is induced, and the complex has good anti-tumor activity.

The invention discloses a method for enhancing a nucleic acid expression effect. The core of the method is that mRNA encoding NADH dehydrogenase (ND) is co-delivered to the same cell with a nucleic acid molecule of interest. The expression product of the ND mRNA provides sufficient energy for translation of target nucleic acid molecules by improving the ATP level in cells, so that the protein expression quantity of the target nucleic acid molecules is remarkably amplified. The method is high in universality, is suitable for various nucleic acid types such as mRNA, circular RNA, self-amplified RNA and DNA, can be widely applied to the fields of vaccines, tumor immunotherapy, gene therapy and the like, and realizes multiplication of the curative effect and saving of the dosage.

This invention relates to the field of natural product extracts and mitochondrial quality control regulation technology, and provides a mitochondrial quality control regulation extract composition derived from red lettuce and its uses. The composition contains active ingredients such as chlorogenic acid, chicoric acid, quercetin glycosides, and anthocyanins, and possesses a stable HPLC fingerprint. Experimental results show that the composition can significantly restore ATP levels in cells damaged by oxidative stress, improve mitochondrial membrane potential and respiratory function, activate AMPK-related pathways, inhibit downstream mTORC1 signaling, enhance mitophagy, and promote mitochondrial regeneration, thereby achieving synergistic regulation of the mitochondrial quality control system. The composition has the advantages of well-defined composition, batch stability, and overall superior effects compared to artificial mixtures, making it suitable for preparing products that regulate mitochondrial function.

The invention belongs to the technical field of biology, and particularly relates to a preparation for in-vitro embryo production and application of the preparation, and the preparation contains at least one of the following components: SEMA4 protein; an Fbxo48 inhibitor; the invention discloses a flavone C-glycoside compound. According to the preparation for in-vitro embryo production, at least one of SEMA4 type protein, Fbxo48 inhibitor and flavone C-glycoside compound is added, so that mitochondrial division and biosynthesis are promoted, mitochondrial functions (including membrane potential, respiratory intensity, ATP level and the like) are enhanced, active oxygen is effectively removed, lipid peroxidation is inhibited and the like; therefore, the maturation quality of in-vitro oocytes and the development potential of in-vitro embryos are remarkably improved in a plurality of stages such as in-vitro maturation of the oocytes, and the application prospect is wide.

The invention provides PQQ keep-alive type mitochondrial microvesicles, a preparation method and application, and belongs to the technical field of cosmetics. The microvesicle is obtained through microfluidic low-oxygen denucleation, PQQ-containing buffer solution wrapping, ultrasonication, gradient ultrasound and PEG surface modification, has high stability, permeability and activity preservation rate, and can avoid inactivation of active components. The ceramide and the lecithin form a flexible liposome, and the flexible liposome wraps a plurality of components such as the PQQ keep-alive type mitochondrial microvesicles, the acetyl hexapeptide and the Ectoine, so that the transdermal absorption type anti-aging composition is formed. Under the high permeability of the flexible liposome, the nano-scale PQQ keep-alive type mitochondrial microvesicles and the like can penetrate through the stratum corneum to deeply nourish skin cells, the transdermal absorption rate is improved by 2.5 times or more, the ATP level is improved, the slow release and long-acting effects of functional components in the skin are ensured, and the effects of smoothing wrinkles and preventing and reversing skin aging are remarkable.

This invention relates to a DNA construct and a vector containing the construct, a cell line transfected with the vector or the construct, isolated mRNA transcribed from the construct, and their use in measuring extracellular ATP levels, the DNA construct comprising the following elements operatively linked from 5' to 3': i) a DNA sequence encoding an extracellular ATP-dependent luciferase; ii) an IRES sequence; and iii) a DNA sequence encoding a cytoplasmic ATP-independent luciferase.

The invention discloses an anti-aging drug screening method based on mitochondrial multilevel analysis-targeted intervention, and particularly relates to the technical field of biological drug manufacturing. The method comprises the following steps: constructing a rotenone-induced aging human mesenchymal stem cell model; the ATP level fluctuation, the active oxygen level, the mitochondrial network fusion and division dynamics and the autophagy flux are monitored in real time by using a fluorescent probe; calculating a baseline function entropy through multi-dimensional data analysis; evaluating the influence of the candidate drugs on the dynamic response entropy of the senescent cells under the condition of mild oxidative stress; an optimal drug is screened and combined with spermidine or urolithin A in a low dosage, and the synergistic effect of the drug in the aspects of maintaining low functional entropy, promoting removal of damaged mitochondria, not causing excessive autophagy and the like is verified. According to the method, multi-dimensional and complete-cycle mitochondrial function evaluation is realized through functional entropy quantification and dynamic stress recovery testing, the method has the advantages of high screening efficiency, objective result and good safety, and a new strategy is provided for the development of anti-aging drugs.

The invention belongs to the technical field of biological medicines, and particularly relates to an anti-aging mitochondrial preparation as well as a preparation method and application thereof. The mitochondrial preparation is formed by compounding mammalian-derived mitochondria and a pharmaceutically acceptable injection solvent, the membrane potential (delta psi m) of the mammalian-derived mitochondria is kept unchanged or reduced by 50%-90% compared with that before separation, and the outer membrane integrity rate is larger than or equal to 90%. Under the state that the membrane potential is reduced, the outer membrane structure is still kept complete, and the capability of inducing host cell PINK1 / Parkin dependent mitochondrial autophagy and network remodeling is achieved, so that inferior mitochondria is selectively removed, and mitochondrial neogenesis is promoted. The preparation can induce systemic mitochondrial quality remodeling in multiple tissues, significantly improve ATP level, reduce ROS generation, increase mitochondrial DNA copy number, down-regulate aging markers p16 and p21, and improve cognition, movement and organ functions in an old animal model.

The present invention provides compounds for treating or preventing a state of decreased intracellular ATP levels.The present invention relates to a compound represented by formula (I):in whichX and Y are the same or different and each represents a substituent selected from Substituent group A: This is the case when the atom connected to the phosphorus atom is oxygen; a group represented by formula 1: —O—R1 in which R1 is selected from the group consisting of a hydrogen, a C1 to C8 alkyl group, a C1 to C3 alkylaryl group, CH3OCH2—, CH3OCH2CH2—, an ester group, a phenyl group, a pyridyl group, a benzyl group, an indolyl group, and a naphthyl group, wherein the aromatic ring or heterocyclic ring thereof may be substituted with one to three of the following functional groups;or a substituent selected from Substituent groups B: This is the case when the atom connected to the phosphorus atom is nitrogen; a group represented by formula 2: —R2—R3 in which R2 is a residue collectively referred to as an aminocarboxylic acid, and R3 is a substituent that binds to the carboxylic acid part of the aminocarboxylic acid in R2; or a pharmaceutically acceptable salt or solvate thereof, and a pharmaceutical composition which comprises the same.

This invention belongs to the technical field of peptide coordination polymer hydrogel materials, and discloses a peptide coordination polymer hydrogel for detecting ATP levels in cancer cells, as well as its preparation method and detection device. The composition provided by this invention includes Ag. + And RGD cyclic peptides, which include free thiol and free guanidinyl groups, Ag + Ag is formed by coordination with RGD cyclic peptide. + -RGD cyclic peptide coordination polymer, Ag + -RGD cyclic peptide coordination polymers can undergo hierarchical assembly to achieve gelation in the presence of ATP or within a gelling concentration range, and the resulting gels exhibit excellent optical and mechanical responsiveness to ATP. Ag exists in solution or gel form. + -RGD cyclic peptide coordination polymers can serve as functional materials for the specific capture and immobilization of cancer cells and ATP detection, showing great promise for real-time-in-situ monitoring of cancer cell activity, evaluation of the therapeutic effects of anticancer drugs, or development of novel tumor diagnostic devices.

The application provides an application of tRF-22 in preparation of a drug for delaying ovarian aging, and a nucleotide sequence of the tRF-22 is TTATTCGACTCCTGGCTGGGC. Through in-vivo and in-vitro experiments, it is proved that the tRF-22 can improve the degeneration of the aging ovarian tissue, reduce the expression of the aging marker, inhibit the secretion of the SASP factor, reverse the aging phenotype of the human ovarian granulosa cell, improve the proliferation activity, relieve the cycle arrest, reduce the apoptosis and the accumulation of the active oxygen. Mechanism research shows that the tRF-22 can inhibit the expression of BNIP3, repair the damage of the mitochondrial ultrastructure, restore the mitochondrial autophagy flux, improve the membrane potential, the ATP level and the oxygen consumption rate, and reshape the mitochondrial homeostasis. The application provides a new active molecule and an action target for the nucleic acid drug for delaying ovarian aging.