Method for improving acid stress resistance of candida glabrata

Abstract

The invention discloses a method for improving the acid stress resistance of candida glabrata, and belongs to the field of bioengineering. The method comprises the following steps: improving the candida glabrata, constructing a gene-deleted mutant strain Cgade12Delta, measuring the intracellular ATP level of the constructed strain, and determining the organic acid tolerance of the strain by a plate growth experiment and cellular activity analysis. After the deletion of the gene CgADE12 is found out, the intracellular ATP level of the strain under the stress of 0.2 percent acetic acid is lowered, the growth capability under the stress of acetic acid is improved, and the acetic acid tolerance is improved.

Description

technical field

[0001] The invention relates to a method for improving the acid stress resistance of Toruula glabrata, belonging to the field of bioengineering. Background technique

[0002] Candida glabrata is the main strain for microbial fermentation to produce pyruvate. In addition, Torulopsis glabrata also has great potential in the production of organic acids such as malic acid, fumaric acid and α-ketoglutaric acid. During the fermentation process, with the accumulation of organic acids, the extracellular pH decreased continuously, and the degree of dissociation of organic acids also continued to decrease, so that they entered the cells in an undissociated state. The higher pH in the cytoplasm dissociates organic acids, releases protons and accumulates, eventually leading to acidification of the cytoplasm and affecting the metabolism and growth of cell metabolism, thereby affecting the fermentation process. In industrial production, usually add a large amount of NaOH...

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