The present application provides a bisborate compound with ROS scavenging activity and cell protection effect, a pharmaceutically acceptable salt, ester, solvate, crystal form or prodrug thereof, a pharmaceutical composition containing the same and uses thereof, and provides an in vitrocell model of an oxidative stress model and a method for screening ROS scavengers using the same. The platform provided by the present application for efficiently screening ROS scavengers has the characteristics of simple operation and good reproducibility, and can be used for drug screening and development of oxidative stress related diseases.