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Protective effect of biochanin A on hippocampal neuron cells

A technology of hippocampal neurons and biochanin, applied to nervous system diseases, medical preparations containing active ingredients, organic active ingredients, etc., can solve problems such as little research

Inactive Publication Date: 2019-09-13
TAISHAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, the interaction between GH and estrogen has been confirmed by many experiments, but there are few studies on the influence of the two on the nervous system

Method used

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Experimental program
Comparison scheme
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Embodiment Construction

[0005] The present invention will be described in detail below in combination with specific embodiments.

[0006] Effects of biochanin A on hydrogen peroxide-induced injury in rat primary hippocampal neurons. Rat hippocampal neuron cells within 24 hours after birth were taken for primary cell culture, and the growth status of the cells was observed under a microscope. After culturing for about 10 days, neuron-specific antibody MAP-2 was added to identify the purity of neuron cells by fluorescence. Bio A with different concentration gradients (10 -7 、10 -6 、10 -5 、10 -4 、10 -3 mol / L) or different concentration gradients of H 2 o 2 (100, 200, 400, 800 μmol / L) stimulated hippocampal neurons for 24 hours, and CCK8 method was used to observe the effect of BioA on the viability of hippocampal neurons. The cultured primary hippocampal cells were divided into normal group, H 2 o 2 group, Bio A low, medium and high dose groups, three dose groups were given according to the scr...

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Abstract

The invention discloses a protective effect of biochanin A on hippocampal neuron cells. The effect of the biochanin A on the injury of primary hippocampal neurons in rats induced by hydrogen peroxideincludes: Bio A preconditioning group can significantly inhibit the injury of hippocampal neurons induced by H2O2; Bio A treatment group can obviously reduce the ROS level in cells; and after Bio A treatment, the invention can up-regulate Bcl-2 protein expression of model cells, and down-regulate Bax and Casepase-3 protein expression dose-dependently.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the protective effect of biochanin A on hippocampal neuron cells. Background technique [0002] Alzheimer's disease (AD), commonly known as senile dementia, belongs to the categories of "dementia", "dementia" and "dementia" in traditional Chinese medicine, and is one of the most common types of senile dementia. Epidemiological and clinical experimental studies at home and abroad have shown that with the decline of estrogen levels in middle-aged and elderly women after menopause, their risk and rate of AD will also increase greatly, and the degree of cognitive impairment in AD patients is significantly higher than that in elderly patients. male. For example, giving estrogen replacement therapy (hormone replacement therapy, HRT) to perimenopausal women (especially women after oophorectomy) can greatly reduce the risk of AD and improve the cognitive function of AD patients. Nevertheless...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P25/00A61P25/28
CPCA61K31/352A61P25/00A61P25/28
Inventor 周延萌宋欣欣
Owner TAISHAN MEDICAL UNIV
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