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Application of Citrus chachiensis seed polymethoxylated flavonoid compound in preparation of antioxidant

A technology of polymethoxylated flavonoids and antioxidants, applied in the fields of application, anti-inflammatory agents, anti-toxic agents, etc., can solve the problems that the medicinal value has not been fully utilized, and achieve good clinical anti-oxidation effects, scavenging free radicals, Reduces the effect of oxidative stress damage

Pending Publication Date: 2022-03-08
SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no development of tea twigs and mandarin oranges into antioxidant drugs, health food or cosmetics in the market, and its medicinal value has not been fully utilized

Method used

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  • Application of Citrus chachiensis seed polymethoxylated flavonoid compound in preparation of antioxidant
  • Application of Citrus chachiensis seed polymethoxylated flavonoid compound in preparation of antioxidant
  • Application of Citrus chachiensis seed polymethoxylated flavonoid compound in preparation of antioxidant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 A kind of preparation method of polymethoxylated flavonoids extract of tea branches and citrus embryos

[0050] (1) Grind and sieve the dried tea twig citrus (collected from Chakeng Village, Xinhui District, Jiangmen City, Guangdong Province) to 14 meshes, and add 95% ( v / v) ethanol aqueous solution assisted by ultrasonic extraction for 3 times (power 300W, frequency 40kHz, time 30min, temperature 40°C), filtered, combined filtrates, concentrated under reduced pressure to obtain extract A1;

[0051] (2) Dissolve extract A1 in water (mass volume ratio of extract A1 to water (kg / L) 1:0.8) to obtain a suspension; add ethyl acetate (volume ratio of ethyl acetate to suspension is 3: 1) extract 3 times, combine ethyl acetate extracts, concentrate under reduced pressure to obtain extract A2;

[0052] (3) Freeze-dry the extract A2 to a constant weight to obtain the polymethoxylated flavonoid extract of Chazhi Citrus.

Embodiment 2

[0053] Embodiment 2 A kind of preparation method of polymethoxylated flavonoids extract of tea branches and citrus embryos

[0054] (1) Grind and sieve the dried tea twig citrus (collected from Chakeng Village, Xinhui District, Jiangmen City, Guangdong Province) to 14 meshes, and add 80% ( v / v) ethanol aqueous solution assisted by ultrasonic extraction 4 times (power 300W, frequency 40kHz, time 30min, temperature 40°C), filtered, combined filtrates, concentrated under reduced pressure to obtain extract A1;

[0055] (2) Dissolve extract A1 in water (mass volume ratio of extract A1 to water (kg / L) 1:0.8) to obtain a suspension; add ethyl acetate (volume ratio of ethyl acetate to suspension is 5: 1) extract twice, combine ethyl acetate extracts, concentrate under reduced pressure to obtain extract A2;

[0056] (3) Freeze-dry the extract A2 to a constant weight to obtain the polymethoxylated flavonoid extract of Chazhi Citrus.

Embodiment 3

[0057] Embodiment 3 A kind of preparation method of polymethoxylated flavonoids extract of tea branches and citrus embryos

[0058] (1) Grind and sieve the dried tea twig citrus (collected from Chakeng Village, Xinhui District, Jiangmen City, Guangdong Province) to 14 meshes, and add 70% ( v / v) ethanol aqueous solution assisted by ultrasonic extraction 5 times (power 300W, frequency 40kHz, time 30min, temperature 40°C), filtered, combined filtrates, concentrated under reduced pressure to obtain extract A1;

[0059] (2) Dissolve extract A1 in water (mass volume ratio of extract A1 to water (kg / L) 1:0.8) to obtain a suspension; add ethyl acetate (volume ratio of ethyl acetate to suspension is 4: 1) extracting 4 times, combining ethyl acetate extracts, concentrating under reduced pressure to obtain extract A2;

[0060] (3) Freeze-dry the extract A2 to a constant weight to obtain the polymethoxylated flavonoid extract of Chazhi Citrus.

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Abstract

The invention belongs to the technical field of plant effective component extraction and application, and discloses application of Citrus chachiensis seed polymethoxylated flavonoid compounds in preparation of antioxidants. The Citrus chachiensis embryo polymethoxylated flavone extract has a protection effect on oxidative damage of cells, can significantly reduce the ROS level in the cells and recover the membrane potential of mitochondria so as to recover the functions of the mitochondria, further reduces oxidative stress damage, shows a good clinical anti-oxidation effect, and has good clinical application prospects. The compound can be applied to various medicines, foods and skin care products with higher requirements on safety, and the oxidation resistance of corresponding products is improved; or independently as an antioxidant; the beverage can also be used as a dietary supplement for removing redundant free radicals in the body and preventing or delaying senescence; or the compound can be used as a medicine or an auxiliary medicine for treating diseases such as neurodegenerative diseases, tumors or inflammations related to oxidative damage.

Description

technical field [0001] The invention belongs to the technical field of extraction and application of effective plant components, and in particular relates to the application of polymethoxylated flavonoids of tea branches and mandarin oranges in the preparation of antioxidants. Background technique [0002] In organisms, there is a redox balance. When the body ages and suffers from disease, this redox balance will be destroyed, shifting to the direction of oxidation, and excessive accumulation of various free radicals and oxides, such as reactive oxygen species. radicals (ROS) and reactive nitrogen radicals (RNS), etc. These oxidative substances are very easy to combine with DNA, protein, phospholipids, etc., causing oxidative damage to various organelles in cells, thereby accelerating the process of the disease. Previous studies have shown that the progression of diseases such as vascular injury diseases, inflammation or tumors are all related to the oxidative damage of cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/752A61K31/352A61P39/06A61P25/00A61P29/00A61P35/00A61K8/9789A61K8/49A61Q19/08A23L33/105A61K131/00
CPCA61K36/752A61K31/352A61P39/06A61P25/00A61P29/00A61P35/00A61K8/9789A61K8/498A61Q19/08A23L33/105A23V2002/00A23V2200/30A23V2200/302A23V2200/308A23V2200/322A23V2250/2116
Inventor 杨得坡张荣菲梅振英赵志敏刘鄂湖
Owner SUN YAT SEN UNIV
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