65results about How to "Reduces oxidative stress damage" patented technology

The invention relates to a wheat germ protein source antioxidative peptide, and a preparation method and an application thereof. The wheat germ protein source antioxidative peptide comprises sequences shown as SEQ ID NO.1 and SEQ ID NO.2. The preparation method comprises the steps of making fresh wheat germs into defatted wheat germ protein powder; hydrolysizing with an alkaline protease, inactivating enzyme, centrifuging at a high speed, subjecting supernatant to ultrafiltration so as to remove large molecular substances, freeze-drying filtrate to obtain a wheat germ protein hydrolysate, passing a wheat germ protein hydrolysate solution through a macroporous absorbent resin column and a gel filtration chromatography column successively, and collecting components with the strongest antioxidant activity being a target product. The preparation method is simple and efficient in process. The obtained wheat germ protein source antioxidative peptide has relatively high antioxidant activity, has relatively small molecular weight and definite amino acid sequence, can clear free radicals and alleviate oxidative stress injuries, can also be used for antioxidant dietary supplements, and has the advantages of wide raw material sources, high security, low cost and the like, and has wide application prospects in the fields of foods, medicines, etc.

The invention provides a lactobacillus plantarum AR113 strain with a protection function on stroke. The lactobacillus plantarum AR113 is preserved in the China General Microbiological Culture Collection Center on March 22, 2017, and the preservation number is CGMCCC No.13909. By adopting the lactobacillus plantarum AR113 strain provided by the invention is capable of improving antioxidant enzyme activities of brain tissue, reducing levels of oxidation products, activating Nrf-ARE signal channels, regulating relative expression amounts of antioxidation factors Nrf2, NQO01 and HO-1, meanwhile, reducing miRNA (micro ribonucleic acid) expression of apoptosis-promoting factors Cyt-C, Caspase-3 and Bax, increasing relative expression of an anti-apoptosis factor Bcl-2, and furthermore, relievingbran cell damage caused by cerebral ischemia reperfusion.

The invention discloses a hydrogel microcapsule for encapsulating a nicotinic acid metal organic framework and a preparation method and application thereof. The preparation method of the hydrogel microcapsule for encapsulating the nicotinic acid metal organic framework comprises the steps that a nicotinic acid solution is used as an inner phase 1; a mixed solution of a zinc acetate solution and acopper acetate solution is used as an inner phase 2; a sodium alginate solution is used as an outer phase; and a microfluidic device is used for spraying mixed liquid drops of the inner phase 1 and the inner phase 2 wrapped by the outer phase into a calcium chloride solution to prepare the hydrogel microcapsule for encapsulating the nicotinic acid metal organic framework. The hydrogel microcapsulefor encapsulating the nicotinic acid metal organic framework, which is prepared by the invention, has ideal controlled release effect, antibacterial effect and anti-oxidative stress effect, and can be used for preparing wound healing materials or medical instruments.

The invention discloses a traditional Chinese medicine composition for treating Parkinson disease. The composition is prepared from 6-15g of cistanche and 6-12g of rhizoma acori graminei, wherein the effect of cistanche in relieving body oxidative stress injury and eliminating free radicals is utilized and is combined with the effect of the rhizoma acori graminei in improving permeability of blood brain barrier to prepare powder, granules, electuary or other traditional Chinese medicine preparations for treating Parkinson disease. Experiments prove that the composition can be used for repairing injured dopaminergic neurons, effectively increasing the content of dopamine in substantia nigra-striatum, up-regulating expression of dopaminergic neurons TH, reducing the degree of brain tissue lipid peroxidation, increasing the antioxidative level in brain and reducing apoptosis of dopaminergic neuron of MPP+ induced Parkinson disease model MES23.5, and does not have remarkable toxins.

The invention belongs to the field of biological medicines, and particularly relates to application of coniferyl aldehyde in the preparation of a medicine for treating neonatal necrotizing enterocolitis. The coniferyl aldehyde provided by the invention inhibits an HMGB1 transcription function by activating an Nrf2 / HO-1 pathway, and alleviates oxidative stress injury of intestinal epithelial cells, so that the neonatal necrotizing enterocolitis is improved. The effect of the Nrf2 / HO-1 / HMGB1 pathway in NEC intestinal epithelial cell oxidative damage and the intervention effect of coniferyl aldehyde are defined, and a new strategy is provided for prevention and treatment of neonatal necrotizing enterocolitis.

The invention discloses a self-heating type temperature partition maintenance instrument. The instrument comprises a hot compress disc, a second handle is arranged at the upper end of the hot compressdisc; a plurality of feeding pipes are arranged in the hot compress disc, a cover plate for sealing the feeding pipes is arranged at the upper ends of the feeding pipes, a first handle is arranged onthe cover plate, a feeding cavity is formed in each feeding pipe, each feeding cavity is filled with a grid, a heating bag is arranged above each grid, a first sponge layer is arranged at the lower end of each heating bag, and the space between each first sponge layer and the corresponding grid is filled with a medicine layer. The self-heating type temperature partition maintenance instrument prepared by the invention has excellent antibacterial and water supplementing properties.

The invention provides an application of water-soluble polysaccharide extracted from a rosaceae rubus plant, rubus suavissimus, in protection of liver and activation of acetaldehyde dehydrogenase.

The invention relates to a traditional Chinese medicine compound resisting duck and rabbit viral liver damage, belonging to the technical field of preparation of traditional Chinese veterinary medicines. The traditional Chinese medicine compound resisting the duck and rabbit viral liver damage is prepared by the following steps of combining herba hyperici japonica, white paeony root, pseudo-ginseng and liquorice according to a certain ratio; adding water in an amount which is 15 times the mass of the mixture; repeatedly decocting for three times; concentrating the decoction solution; and subpackaging and sterilizing. An artificial infection method discovers that after the compound is applied, the death rate of ducks suffering from viral hepatitis and the death rate of rabbits suffering from viral hemorrhagic disease are respectively reduced by 22.2% and 30% compared with those of the non-treatment control groups, the course of diseases is shortened by 1 to 2 days, the content of viruses in the animals after counteraction to toxic substances is obviously reduced, and the change of duck and rabbit liver damage biochemical indexes and oxidative stress injury caused by virus attack are effectively reduced. The traditional Chinese medicine compound has a good treatment effect on duck viral hepatitis and rabbit viral hemorrhagic disease, effectively reduces duck and rabbit liver damage caused by infection of the two viruses, and is simple in component and preparation process and low in cost.

The invention discloses a preparation method of a thermally responsive bladder extracellular matrix bioactive hydrogel, relates to the field related to bioactive hydrogel preparation methods, and solves the problems that most of existing methods for obtaining an extracellular matrix (ECM) in the prior art have the defects of insufficient elution and large damage to ECM, and preparation of bladder-derived ECM hydrogels has not been reported so far. The preparation method comprises the following steps: step 1: taking a fresh pig bladder, keeping the ureter and urethral structure, and conductingrinsing with 3000 mL of double distilled water for 2 h; step 2: after wrapping by tin foil paper, quickly place the wrapped object in a refrigerator of -80 DEG C for 1 hour, then taking the object outand balancing the object to room temperature; step 3: repeating the step two for two times; step 4: conducting perfusion and immersing by double distilled water for 4 h, and immersing the whole bladder in a perfusate; and step 5: conducting perfusion and immersing by 1% Triton X-100 for 4 h, and immersing the whole bladder in a 1% Triton X-100 perfusate, and then conducting rinsing by double distilled water for 20 min.

The invention discloses anti-oxidative magnetic iron oxide nanoparticles which comprise magnetic mesoporous silicon dioxide nanoparticles with a core-shell structure formed by coating the surface of an Fe3O4 inner core with mesoporous silicon dioxide, and also comprise an antioxidant N-acetylcysteine, wherein the antioxidant N-acetylcysteine is supported in mesoporous silicon dioxide. The invention also provides a preparation method of the anti-oxidative magnetic iron oxide nanoparticles. The method comprises the steps of preparing an antioxidant N-acetylcysteine solution; weighing a magneticmesoporous silicon dioxide nanoparticle solution with the core-shell structure, executing centrifugation, then adding the antioxidant N-acetylcysteine solution; sealing with a sealing film after ultrasonic treatment, placing the solution into a shaking table to shake, taking out a sample after completion, and centrifuging and drying the sample to obtain the anti-oxidative magnetic iron oxide nanoparticles. After the anti-oxidative magnetic iron oxide nanoparticles reach a target part, the antioxidant is released, so that the oxidative stress damage mediated by the iron oxide is relieved, and the histocompatibility of the magnetic iron oxide nanoparticles is improved.