The invention belongs to the field of drug processing and particularly relates to a minimal hepatic encephalopathy intestinal flora regulating Chinese herbal preparation and a preparation method thereof. The minimal hepatic encephalopathy intestinal flora regulating Chinese herbal preparation is mainly composed of kudzuvine root, coptis chinensis, red date, honeysuckle, red ginseng, semen coicis,ginsenoside, astragalus polysaccharides, gynostemma pentaphylla, clove tea extract, radix paeoniae alba, liquorice, eryngo, timosaponin, catechinic acid, apigenin, fructus evodiae, brucea javanica, lobelia chinensis, amaranthus retroflexus, frankincense, eucalyptus honey and lichen. The minimal hepatic encephalopathy intestinal flora regulating Chinese herbal preparation can accelerate intestinalperistalsis and promote defecation; during defecation, intestinal pernicious bacteria and unhealthy metabolites are excreted to improve intestinal flora disequilibrium; contained organic acids maintain an acid intestinal environment and reduce the pH of intestinal tracts to provide a suitable growing environment for intestinal probiotics. The minimal hepatic encephalopathy intestinal flora regulating Chinese herbal preparation can inhibit bacteria by damaging cytoderm synthesis, protein synthesis and genetic material synthesis of the bacteria.