141 results about "Choleic Acid" patented technology

Provided is the use of a conjugated cholic acid, tryptophan-conjugated bile acids (TrpCA) or a TrpCA-producing strain for preventing and / or treating a metabolic disease. The present disclosure relates to the use of tryptophan-conjugated cholic acid or its producing bacteria strain in the preparation of a composition for preventing and / or treating a metabolic disease, or for promoting GLP-1 and / or insulin production. The inventors have discovered for the first time that the tryptophan-conjugated cholic acid can significantly promote the secretion of GLP-1 in both in vivo animal model and in vitro cell model, thereby reducing weight, lowering blood glucose and relieving symptoms of non-alcoholic fatty liver disease. Moreover, colonization of the selected probiotics can also have the same metabolic improvement effects.

There is provided the use of Lactobacillus paracasei or a afterbiogen in the preparation of a pharmaceutical composition or a food composition for the production of tryptophan-binding cholic acid. The Lactobacillus paracasei or the afterbiogen thereof can be used to prevent and / or treat metabolic diseases or to promote the production of GLP-1 and / or insulin, or to maintain blood glucose healthy levels, to maintain blood lipid health and / or to control fat in the body.

The invention provides application of combined cholic acid TrpCA and producing bacteria thereof in treating and preventing metabolic diseases. The present invention relates to the use of tryptophan-binding cholic acid or a producing bacterium thereof in the preparation of a composition for preventing and / or treating a metabolic disease or for promoting the production of GLP-1 and / or insulin. The inventor finds for the first time that tryptophan-bound cholic acid can remarkably promote secretion of GLP-1 on an in-vivo animal model and an in-vitro cell model, the effects of reducing weight, reducing blood sugar and relieving the symptoms of the non-alcoholic fatty liver disease are achieved, and the same metabolism improvement effect can also be achieved through planting of probiotics capable of generating cholic acid TrpCA.

The invention discloses a culture solution for reducing lipid of animal embryos before freezing as well as a preparation method and application of the culture solution. The culture solution is prepared by adding bezafibrate and cholic acid into an NCSU23 culture solution. The invention also discloses a preparation method of the culture solution, which comprises the following steps: preparing an NCSU23 culture solution, and adding bezafibrate and cholic acid into the NCSU23 culture solution. Compared with a conventional NCSU23 culture solution, the culture solution provided by the invention has the advantages that the added bezafibrate and cholic acid have a synergistic lipid-lowering effect, so that the content of lipid in an embryo can be remarkably reduced, and the survival rate and the blastocyst hatching rate after the embryo is frozen and unfrozen are finally effectively improved. The culture solution provided by the invention has application prospects in the aspects of animal embryo lipid reduction, frozen embryo survival and development and the like.

The disclosure relates to a traditional Chinese medicine composition, comprising active ingredients derived from Cornu caprae or Cornu saigae tataricae, Radix scutellariae, Bulbus fritillariae ussuriensis, Radix glycyrrhizae, Radix et rhizoma rhei, Gypsum fibrosum, Calculus bovis artifactus and Lapis Chloriti, the said active ingredients comprise in parts by weight: at least 1600 parts of amino acids; at least 120 parts of gallic acid; at least 130 parts of liquiritin; at least 20 parts of liquiritigenin; at least 400 parts of baicalin; at least 40 parts of oroxyloside; at least 120 parts of wogonoside; at least 280 parts of glycyrrhizic acid; at least 12 parts of chrysin-7-O-β-D-glucoronic acid; at least 50 parts of aloe-emodin-8-O-β-D-glucopyranoside; at least 30 parts of chrysophanol-1-O-β-D-glucopyranoside; at least 45 parts of chrysophanol-8-O-β-D-glucopyranoside; at least 260 parts of hyodeoxycholic acid; at least 150 parts of cholic acid. The traditional Chinese medicine composition in the present invention possess antipyretic, anti-inflammatory and antitussive effects.

The invention belongs to the field of medicinal chemistry, and particularly discloses application of oxime cholic acid analogues as TGR5 allosteric agonists. The preparation method comprises the following steps: by taking cholic acid as a raw material, obtaining cholic acid intermediates of which the 12th site or the 7th site and the 12th site are oximes through a series of reactions, and then carrying out a series of modification on the 24th site and oximes of the intermediates to obtain the cholic acid analogue as shown in a formula 1 or a formula 2. According to the invention, a GloSensor cAMP accumulation experiment is adopted to test the functional activity and allosteric regulation mechanism of all compounds on TGR5 on a G protein dependent signal channel. Pharmacological results show that all the synthesized target compounds have relatively good agonist activity on TGR5, and can positively allosteric regulate the functional activity of chenodeoxycholic acid CDCA, that is, the target compounds disclosed by the invention are all TGR5 positive allosteric modulators or allosteric agonists. # imgabs0 # imgabs1 #

Provided are methods of treating diabetes and / or obesity in a subject in need thereof, and methods of increasing the amount of cholic acid-7-sulfate (CA7S) in a subject. Further provided herein are methods of administering CA7S to a subject. Also provided are compositions and kits comprising cholic acid-7-sulfate, or a salt thereof for use in the treatment of diabetes and / or obesity.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus cereus as well as a culture and application thereof. The preservation number of the bacillus cereus AK-LY1 is CCTCC (China Center For Type Culture Collection) NO: M 2025756. The bacillus cereus AK-LY1 disclosed by the invention is excellent in trypsin resistance, good in cholate resistance, acid resistance and high temperature resistance, good in inhibition performance on antibiotics such as strong penicillin and chloramphenicol, and relatively good in adhesion capacity in intestinal tracts, and provides a relatively good microecological preparation reference basis for expanding a bacillus cereus resource library and applying the bacillus cereus.

The invention relates to an exosome fusion liver targeting liposome drug delivery system as well as a preparation method and application thereof, and belongs to the field of high polymer material science and pharmaceutical preparations. The invention discloses an exosome-fused liver-targeted liposome drug delivery system, which combines high-concentration PEG8000 and Nycodenz in a breakthrough manner, synergistically improves the fusion efficiency of exosome and liposome, and forms bionic vesicles with uniform size and high drug loading capacity. The exosome not only can exert the anti-inflammatory and anti-oxidation characteristics, but also plays a role in resisting a severe gastrointestinal environment and crossing a biological barrier. In addition, liposome is modified by cholic acid derivatives through EDC / NHS mediated amidation reaction, precise targeting of the liver is achieved, and the liposome is fully accumulated in the liver. The effects synergistically realize the treatment of type II diabetes and non-alcoholic steatohepatitis.

The invention relates to a construction method and application of a bile acid fingerprint spectrum in bile, and relates to the technical field of traditional Chinese medicine detection. The construction method comprises the following steps: preparing a reference substance solution: weighing at least two of taurocholic acid, taurodeoxycholic acid, glycocholic acid, cholic acid and glycodeoxycholic acid reference substances, and preparing the reference substance solution; preparing a test sample solution, namely filtering bile, taking filtrate, heating in a gradient manner, drying and grinding to obtain a test sample, and preparing the test sample solution; and constructing a fingerprint spectrum: respectively injecting the reference substance solution and the test solution into a high performance liquid chromatograph, and determining under a predetermined liquid phase condition to construct the bile acid fingerprint spectrum. The construction method can be used for fully and effectively separating and identifying various bile acid components in the bile, can be used for simultaneously detecting and measuring the various bile acid components in the bile, and has important significance in the aspects of quality control, authenticity identification, basic research on pharmacodynamic substances and the like of the bile.

The invention provides a silibinin mixed micelle injection as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. The cholate / lecithin mixed micelle system constructed by adopting specific cholic acid and phospholipid has good stability, can be well compatible with the silibinin so as to well encapsulate the silibinin, can obviously improve the solubility of the silibinin, has higher safety under the condition of ensuring the stability of the silibinin, and can be used for preparing a medicine for treating the silibinin. Therefore, the bioavailability of the silibinin is greatly improved. According to the invention, a shearing dispersion method is adopted to prepare the mixed micelles, and the mixed micelles with high drug loading capacity, clarification and good stability for silibinin are formed by destroying and shearing the raw and auxiliary materials in a molecular level, so that the mixed micelles have better physical and chemical stability, the drug quality is easier to control, the process is more simplified, the clinical medication is safer and the cost is lower; and the accessibility of the medicine is improved.

Methods for stereo-selectively crystallizing 3-keto-5β-cholanic acid ("3-KCA") and 3-keto ursodeoxycholic acid ("3-KUDCA") of exceptional purity, salts useful in carrying out such methods, particularly the t-butylamine and cyclohexylamine salts of 3-KCA, the t-butylamine salt of 3-KUDCA, and crystalline forms of such salts, and methods of making commercial steroids from such purified materials.

The invention discloses a method for extracting bilirubin, cholic acid and chenodeoxycholic acid from chicken bile. The method comprises the steps of pretreatment, chicken bile treatment, hydrolysis reduction, chromatographic separation and the like. The method comprises the following steps: slicing frozen chicken gallbladder, heating, unfreezing and melting to denature protein into solid, then cooling, performing three-phase centrifugal degreasing and deproteinization, and adding three enzymes including biliverdin reductase, coenzyme I and glucose dehydrogenase to reduce free biliverdin and combined biliverdin, so as to obtain the biliverdin-free biliverdin-glucose dehydrogenase-free biliverdin-glucose dehydrogenase-free biliverdin-glucose dehydrogenase. The method comprises the following steps: adding cholic acid and chenodeoxycholic acid into a container, adding gluconic acid hydrolase and bile salt hydrolase to hydrolyze biliverdin and bile acid, adding trypsin to hydrolyze impure protein, performing chromatography by using macroporous silica gel, and separating bilirubin, cholic acid and chenodeoxycholic acid by using different molecular space structures to obtain high-purity bilirubin, cholic acid and chenodeoxycholic acid. The method has the advantages of simple process, few types of used solvents, low toxicity, and facilitation of environmental protection and industrial production.

The invention relates to a preparation method of bionic calculus bovis, the bionic calculus bovis and application of the bionic calculus bovis, and belongs to the technical field of traditional Chinese medicine raw material preparation. According to the preparation method, natural oxgall is used as a fermentation substrate, a fermentation and / or enzymatic reaction system is used for preparing the bionic calculus bovis, and the fermentation and / or enzymatic reaction system is selected from at least one of synthetase plasmids, synthetase high-expression engineering bacteria and calculus bovis forming bacteria as a fermentation strain for direct in-vitro fermentation. The strains of the bezoar forming bacteria, the carriers of the synthetase high-expression engineering bacteria and the specific types of the synthetase plasmids are described in the specification. The bionic calculus bovis prepared by the method is highly consistent with natural calculus bovis in component, the content of cholic acid (CA) is 8.5%-20.0%, the content of deoxycholic acid (DCA) is 2.0%-6.5%, the fingerprint similarity is greater than or equal to 88.0%, and pharmacological experiments show that the bionic calculus bovis prepared by the method has the same acting effect as the natural calculus bovis and is superior to that of each pair of calculus bovis.

The invention discloses a method for reducing the lipid content of an oocyte or increasing the freezing survival rate of the oocyte. The method comprises the following steps: (1) preheating an incubation solution; incubating the oocytes in the preheated incubation liquid; (2) freezing and unfreezing the incubated oocytes; wherein the incubation liquid is prepared by adding bezafibrate and cholic acid into a culture medium 199 containing 10wt% of fetal calf serum. Compared with a conventional culture medium 199 containing 10 wt% of fetal calf serum, the incubation liquid used in the method provided by the invention has the advantages that bezafibrate and cholic acid which are added have a synergistic lipid-lowering effect, so that the lipid content of the oocytes can be remarkably reduced or the freezing survival rate of the oocytes can be increased by the method. The method has application prospects in the aspects of reducing the lipid content of the oocytes or improving the freezing survival rate of the oocytes and the like.

The invention provides a paclitaxel molecular umbrella delivery system prodrug as well as a preparation method and application thereof. The paclitaxel molecular umbrella delivery system prodrug comprises a molecular umbrella target head part which is formed by connecting two surface amphiphilic molecules through spermidine; a paclitaxel drug moiety; and a linking group that links the molecular umbrella target head portion to the paclitaxel drug portion through a disulfide bond; wherein the surface amphiphilic molecule is selected from cholic acid, deoxycholic acid or sodium sulfonate thereof. Compared with paclitaxel, the paclitaxel molecular umbrella delivery system prodrug has the advantages that the water solubility is obviously improved, the toxicity to normal cells is lower than that of free paclitaxel, and the paclitaxel molecular umbrella delivery system prodrug has obviously improved safety; the paclitaxel molecular umbrella delivery system prodrug has the following characteristics in a physiological environment: an active drug is released in a high GSH / ROS microenvironment of tumor cells; and maintaining the prodrug state in normal cells.

The application relates to the technical field of biological pharmacy, and discloses a method for preparing high-bionic bovine bezoar by using a 3D printing technology and application. The preparation raw material of the high-bionic bovine bezoar comprises the following components: fermented bovine bile, saturated calcium hydroxide, hydroxypropyl methyl cellulose, bilirubin, cholic acid, deoxycholic acid and taurine; wherein the fermented bovine bile is obtained by fermenting bovine bile by using escherichia coli. The high-bionic bovine bezoar prepared by using the preparation raw material to perform 3D printing can be comparable to natural bovine bezoar in terms of components, effects and appearance, the main pharmaceutical components can be adjusted according to actual requirements, the production cost of the bovine bezoar can be greatly reduced, and the demand of people for the bovine bezoar can be met.

The application belongs to the field of neuropharmacology and medicine, and particularly relates to application of taurine cholic acid in preparation of a drug for preventing or treating Alzheimer's disease. Through animal model experiment verification, the A beta lateral ventricle injection is used to prepare an AD mouse model, and after oral administration of TCA for 2 weeks, cognitive behavior and pathological indexes are significantly improved.

A method for determining the presence of prostate cancer in a subject, the method comprising the steps of: (i) determining the level of one or more biomarkers in a sample from the subject; (ii) compar

The invention provides a butylphthalide injection and a preparation method thereof, and relates to the technical field of medicines.The butylphthalide injection comprises butylphthalide, cholate, phospholipid and water for injection, the cholate is prepared through a reaction of cholic acid and sodium hydroxide; the molar ratio of the phospholipid to the cholic acid is (0.3-1.2): 1. The mass fraction of butylphthalide in the butylphthalide injection is 0.025%-1%, the butylphthalide, cholate and phospholipid are mixed and proportioned under the conditions of certain concentration and mass ratio and cholic acid and phospholipid in the cholate under the condition of certain molar ratio, and the butylphthalide injection of a clear solution is prepared; the preparation process of the butylphthalide injection is simple, the stability of the injection is further improved through repeated dissolving and stirring, clinical medication requirements are met, and industrial production is facilitated.

An esketamine liquid preparation. The esketamine liquid preparation is an aqueous solution agent, comprising esketamine or a pharmaceutically acceptable salt thereof, cholic acid or a derivative thereof, and water, with a pH value of 2.5-5.7. The esketamine liquid preparation is administrated via oral mucosa, and the preparation is high in stability, fast in absorption, high in bioavailability, simple in administration mode, and good in compliance. The esketamine liquid preparation can be used for preventing, relieving, or treating major depression, unipolar depression, treatment-refractory depression, intractable depression, agitated depression, and bipolar depression.

The invention belongs to the technical field of detection and analysis, and provides an HPLC detection method for taurocholic acid in snake gall bulbus fritilariae liquid, which comprises the following steps: collecting chromatograms of a test sample and a reference substance through HPLC, and judging whether a co-outflow phenomenon exists or not; if the co-outflow phenomenon occurs, interference proportion analysis is carried out, and main interferents are identified; the judged main interferents are removed; carrying out retention analysis to obtain a taurocholic acid retention rate, judging whether the taurocholic acid retention rate accords with expectation or not based on the taurocholic acid retention rate, and if not, judging whether a trailing signal is triggered or not; if the trailing signal is triggered, performing cause analysis to determine the cause of triggering the trailing signal; when the optimal methanol proportion is obtained, a comprehensive detection signal is triggered; if the comprehensive detection signal is triggered, performing comprehensive adaptation analysis of the HPLC detection method to obtain an adaptation index, and evaluating the comprehensive performance of the HPLC detection method according to the adaptation index; and the accuracy of HPLC taurocholic acid detection is effectively improved.

The application provides a preparation method and application of a near-infrared two-region fluorescent sensor, and chiral single-walled carbon nanotubes are separated by using a double water phase extraction method; materials in the separation process include deionized water, polyethylene glycol, dextran, sodium dodecyl sulfate, sodium deoxycholate and cholic acid sodium hydrate; the chiral single-walled carbon nanotubes are dispersed and mixed with 4-nitrobenzene tetrafluoroboric acid diazonium salt to obtain the fluorescent sensor; high-purity (6,5)-SWCNT is efficiently and simply separated by using the double water phase extraction technology, the prepared SWCNT-NBD nanomaterial has good optical performance, including bright light emission in the near-infrared two-region, has good penetration capacity on biological tissues, the fluorescent detector (SWCNT-NBD) has low detection line, wide linear range, good selectivity on DA, and is convenient to prepare and low in cost.

The invention discloses a cholic acid derivative as well as a preparation method and application thereof. The cholic acid derivative is prepared by the following steps: dissolving bile acid in vinyl ethyl ether, adding a catalyst, reacting at room temperature, removing acidic components and byproducts, filtering, evaporating and drying. Vinyl ethyl ether is used for modifying carboxyl side chains and hydroxyl groups of bile acid, so that the bile acid is endowed with higher hydrophobicity, and a structure with carboxyl and hydroxyl can be quickly recovered when the bile acid is in contact with water. The cholic acid derivative can spontaneously form a stable emulsion in a self-emulsifying drug delivery system, the solubility and bioavailability of drugs are improved, good biocompatibility is kept, and the cholic acid derivative is suitable for delivery of various drugs and has good application prospects. And the problems that the emulsifying time in the existing self-emulsifying system is relatively long, a toxic nonionic surfactant and a toxic cosolvent need to be used and the like are also solved.

The utility model relates to the technical field of chromatographic separation devices, and discloses a cholic acid chromatographic separation device which comprises a supporting plate, an anti-skid cushion block is fixedly connected to the lower surface of the supporting plate, a fixing rod is fixedly connected to the interior of the supporting plate, a sliding rod is connected to the outer wall of the fixing rod in a sliding mode, a plurality of second grooves are formed in the sliding rod, and a plurality of second grooves are formed in the second grooves. An adjusting ring is fixedly connected to the outer wall of the sliding rod, a hinge seat is fixedly connected to the outer wall of the adjusting ring, a rotating rod is rotatably connected to the interior of the hinge seat, a spring clamping block is arranged in the rotating rod, a plug pin is slidably connected to the interior of the adjusting ring, and the outer wall of the plug pin is slidably connected to the inner wall of one second groove. According to the utility model, the sliding rod slides on the fixed rod, the slotted hole in the adjusting ring is aligned with the groove in the proper position and is fixed by the bolt, and the spring clamping block connected with the rotating rod is clamped in the clamping groove, so that the sliding rod is supported and assisted in further adjustment, the operation difficulty is reduced, and the working efficiency is improved.

The invention belongs to the technical field of biomedicine synthesis, and particularly relates to the technical field of chemical synthesis of natural drugs / prodrugs for controlling metabolic abnormalities such as blood sugar and blood fat. The invention provides a preparation method of a cholic acid derivative. The preparation method comprises the following steps: adding benzyl bromide into an alkaline solution containing cholic acid to obtain benzyl cholate; the method comprises the following steps: adding a dichloromethane solution containing acyl chloride into a dichloromethane solution containing benzyl cholate and 4-dimethylaminopyridine under protective gas to obtain a colorless oily product; and introducing hydrogen into the absolute ethyl alcohol in which the colorless oily product is dissolved to obtain the cholic acid derivative. The chemical synthesis of the cholic acid derivative is realized.

The utility model relates to the technical field of cholic acid purification, in particular to a cholic acid purification device which comprises a bottom plate, a centrifugal device fixedly connected to the top of the bottom plate through a second fixing rod, a first filtering box body fixedly connected to the top of the bottom plate through a first fixing rod, and a second filtering box body fixedly connected to the first filtering box body through a fifth fixing rod. The second filtering box body is fixedly connected with a distillation box body through a fourth fixing rod, the distillation box body is fixedly connected with a condensation recovery box body through a condensation pipe, a stirring barrel is fixedly installed at the top of the first filtering box body, a bile feeding port and an ethanol feeding port are formed in the top of the stirring barrel, and a first telescopic rod stretches out and draws back to drive a first long strip to move. Filtering of the bile and ethanol mixed solution is accelerated, the ethanol and cholic acid mixed solution generated by the first filtering box body, the second filtering box body and the condensation recycling box body is centrifugally layered, the centrifugally layered liquid flows into the drying box to dry the ethanol solution, and finally cholic acid is obtained.

The invention belongs to the technical field of biochemical pharmacy, and particularly relates to a compound bezoar composition, decoction piece type compound bezoar and a preparation method of the decoction piece type compound bezoar. The compound calculus bovis composition provided by the invention comprises 94-96% by mass of main agents and 8 kinds of calculus bovis intermediates, the mass content of the auxiliary agent is 4-6%, and the auxiliary agent is six commercially available finished product combined cholic acid. The bilirubin content of the bezoar intermediate used in the invention is increased by more than 30% and the yield is increased by more than 25% compared with the existing biochemical reaction process of oxgall. The DPPH free radical scavenging rate of the compound bezoar composition reaches 89.6% + / -2.1% and is increased by 25.7-36.9% compared with 58.3% + / -3.5% of a product prepared from ox bile, and through t inspection, p is smaller than 0.01 (n is equal to 5). Free cholic acid intervention is strictly prohibited, lithocholic acid addition is completely eradicated, and the composition has the advantages of being high in bilirubin content, high in oxidation resistance and high in biological activity and adapts to the physiological characteristics of the human body.

The invention belongs to the technical field of separation equipment, and particularly relates to separation equipment of a cholic acid extraction reaction kettle and a separation method thereof, the separation equipment comprises a kettle base assembly, a separation assembly and a stirring assembly; the kettle base assembly is of an open structure, a kettle cover is clamped to the top of the kettle base assembly, a servo motor is embedded in the top of the kettle cover, and the separation assembly is slidably connected to the inner wall of the kettle base assembly; the stirring assembly is driven by the output end of the servo motor to rotate, so that the stirring assembly stirs raw materials for cholic acid extraction reaction in the separation assembly, in the process that the separation assembly slides along the inner wall of the kettle base assembly, the separation assembly and the stirring assembly are separated, and the stirring assembly is switched to a cholic acid screening state; the stirring assembly is continuously driven by the servo motor to rotate, solid or liquid of cholic acid in the separation assembly is separated, and the stirring or separation state switching use efficiency of the reaction kettle is improved.

The present invention relates to a process for the preparation of cholic acid derivatives, in particular compounds of formula (V). The invention also relates to compounds useful for the preparation of such derivatives. # imgabs0 #