118 results about "Choleic Acid" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus cereus as well as a culture and application thereof. The preservation number of the bacillus cereus AK-LY1 is CCTCC (China Center For Type Culture Collection) NO: M 2025756. The bacillus cereus AK-LY1 disclosed by the invention is excellent in trypsin resistance, good in cholate resistance, acid resistance and high temperature resistance, good in inhibition performance on antibiotics such as strong penicillin and chloramphenicol, and relatively good in adhesion capacity in intestinal tracts, and provides a relatively good microecological preparation reference basis for expanding a bacillus cereus resource library and applying the bacillus cereus.

The invention relates to an exosome fusion liver targeting liposome drug delivery system as well as a preparation method and application thereof, and belongs to the field of high polymer material science and pharmaceutical preparations. The invention discloses an exosome-fused liver-targeted liposome drug delivery system, which combines high-concentration PEG8000 and Nycodenz in a breakthrough manner, synergistically improves the fusion efficiency of exosome and liposome, and forms bionic vesicles with uniform size and high drug loading capacity. The exosome not only can exert the anti-inflammatory and anti-oxidation characteristics, but also plays a role in resisting a severe gastrointestinal environment and crossing a biological barrier. In addition, liposome is modified by cholic acid derivatives through EDC / NHS mediated amidation reaction, precise targeting of the liver is achieved, and the liposome is fully accumulated in the liver. The effects synergistically realize the treatment of type II diabetes and non-alcoholic steatohepatitis.

Methods for stereo-selectively crystallizing 3-keto-5β-cholanic acid ("3-KCA") and 3-keto ursodeoxycholic acid ("3-KUDCA") of exceptional purity, salts useful in carrying out such methods, particularly the t-butylamine and cyclohexylamine salts of 3-KCA, the t-butylamine salt of 3-KUDCA, and crystalline forms of such salts, and methods of making commercial steroids from such purified materials.

The invention discloses a method for extracting bilirubin, cholic acid and chenodeoxycholic acid from chicken bile. The method comprises the steps of pretreatment, chicken bile treatment, hydrolysis reduction, chromatographic separation and the like. The method comprises the following steps: slicing frozen chicken gallbladder, heating, unfreezing and melting to denature protein into solid, then cooling, performing three-phase centrifugal degreasing and deproteinization, and adding three enzymes including biliverdin reductase, coenzyme I and glucose dehydrogenase to reduce free biliverdin and combined biliverdin, so as to obtain the biliverdin-free biliverdin-glucose dehydrogenase-free biliverdin-glucose dehydrogenase-free biliverdin-glucose dehydrogenase. The method comprises the following steps: adding cholic acid and chenodeoxycholic acid into a container, adding gluconic acid hydrolase and bile salt hydrolase to hydrolyze biliverdin and bile acid, adding trypsin to hydrolyze impure protein, performing chromatography by using macroporous silica gel, and separating bilirubin, cholic acid and chenodeoxycholic acid by using different molecular space structures to obtain high-purity bilirubin, cholic acid and chenodeoxycholic acid. The method has the advantages of simple process, few types of used solvents, low toxicity, and facilitation of environmental protection and industrial production.

The invention discloses a method for reducing the lipid content of an oocyte or increasing the freezing survival rate of the oocyte. The method comprises the following steps: (1) preheating an incubation solution; incubating the oocytes in the preheated incubation liquid; (2) freezing and unfreezing the incubated oocytes; wherein the incubation liquid is prepared by adding bezafibrate and cholic acid into a culture medium 199 containing 10wt% of fetal calf serum. Compared with a conventional culture medium 199 containing 10 wt% of fetal calf serum, the incubation liquid used in the method provided by the invention has the advantages that bezafibrate and cholic acid which are added have a synergistic lipid-lowering effect, so that the lipid content of the oocytes can be remarkably reduced or the freezing survival rate of the oocytes can be increased by the method. The method has application prospects in the aspects of reducing the lipid content of the oocytes or improving the freezing survival rate of the oocytes and the like.

The invention provides a paclitaxel molecular umbrella delivery system prodrug as well as a preparation method and application thereof. The paclitaxel molecular umbrella delivery system prodrug comprises a molecular umbrella target head part which is formed by connecting two surface amphiphilic molecules through spermidine; a paclitaxel drug moiety; and a linking group that links the molecular umbrella target head portion to the paclitaxel drug portion through a disulfide bond; wherein the surface amphiphilic molecule is selected from cholic acid, deoxycholic acid or sodium sulfonate thereof. Compared with paclitaxel, the paclitaxel molecular umbrella delivery system prodrug has the advantages that the water solubility is obviously improved, the toxicity to normal cells is lower than that of free paclitaxel, and the paclitaxel molecular umbrella delivery system prodrug has obviously improved safety; the paclitaxel molecular umbrella delivery system prodrug has the following characteristics in a physiological environment: an active drug is released in a high GSH / ROS microenvironment of tumor cells; and maintaining the prodrug state in normal cells.

The application relates to the technical field of biological pharmacy, and discloses a method for preparing high-bionic bovine bezoar by using a 3D printing technology and application. The preparation raw material of the high-bionic bovine bezoar comprises the following components: fermented bovine bile, saturated calcium hydroxide, hydroxypropyl methyl cellulose, bilirubin, cholic acid, deoxycholic acid and taurine; wherein the fermented bovine bile is obtained by fermenting bovine bile by using escherichia coli. The high-bionic bovine bezoar prepared by using the preparation raw material to perform 3D printing can be comparable to natural bovine bezoar in terms of components, effects and appearance, the main pharmaceutical components can be adjusted according to actual requirements, the production cost of the bovine bezoar can be greatly reduced, and the demand of people for the bovine bezoar can be met.

The application belongs to the field of neuropharmacology and medicine, and particularly relates to application of taurine cholic acid in preparation of a drug for preventing or treating Alzheimer's disease. Through animal model experiment verification, the A beta lateral ventricle injection is used to prepare an AD mouse model, and after oral administration of TCA for 2 weeks, cognitive behavior and pathological indexes are significantly improved.

A method for determining the presence of prostate cancer in a subject, the method comprising the steps of: (i) determining the level of one or more biomarkers in a sample from the subject; (ii) compar

The invention belongs to the technical field of detection and analysis, and provides an HPLC detection method for taurocholic acid in snake gall bulbus fritilariae liquid, which comprises the following steps: collecting chromatograms of a test sample and a reference substance through HPLC, and judging whether a co-outflow phenomenon exists or not; if the co-outflow phenomenon occurs, interference proportion analysis is carried out, and main interferents are identified; the judged main interferents are removed; carrying out retention analysis to obtain a taurocholic acid retention rate, judging whether the taurocholic acid retention rate accords with expectation or not based on the taurocholic acid retention rate, and if not, judging whether a trailing signal is triggered or not; if the trailing signal is triggered, performing cause analysis to determine the cause of triggering the trailing signal; when the optimal methanol proportion is obtained, a comprehensive detection signal is triggered; if the comprehensive detection signal is triggered, performing comprehensive adaptation analysis of the HPLC detection method to obtain an adaptation index, and evaluating the comprehensive performance of the HPLC detection method according to the adaptation index; and the accuracy of HPLC taurocholic acid detection is effectively improved.

The application provides a preparation method and application of a near-infrared two-region fluorescent sensor, and chiral single-walled carbon nanotubes are separated by using a double water phase extraction method; materials in the separation process include deionized water, polyethylene glycol, dextran, sodium dodecyl sulfate, sodium deoxycholate and cholic acid sodium hydrate; the chiral single-walled carbon nanotubes are dispersed and mixed with 4-nitrobenzene tetrafluoroboric acid diazonium salt to obtain the fluorescent sensor; high-purity (6,5)-SWCNT is efficiently and simply separated by using the double water phase extraction technology, the prepared SWCNT-NBD nanomaterial has good optical performance, including bright light emission in the near-infrared two-region, has good penetration capacity on biological tissues, the fluorescent detector (SWCNT-NBD) has low detection line, wide linear range, good selectivity on DA, and is convenient to prepare and low in cost.

The invention discloses a cholic acid derivative as well as a preparation method and application thereof. The cholic acid derivative is prepared by the following steps: dissolving bile acid in vinyl ethyl ether, adding a catalyst, reacting at room temperature, removing acidic components and byproducts, filtering, evaporating and drying. Vinyl ethyl ether is used for modifying carboxyl side chains and hydroxyl groups of bile acid, so that the bile acid is endowed with higher hydrophobicity, and a structure with carboxyl and hydroxyl can be quickly recovered when the bile acid is in contact with water. The cholic acid derivative can spontaneously form a stable emulsion in a self-emulsifying drug delivery system, the solubility and bioavailability of drugs are improved, good biocompatibility is kept, and the cholic acid derivative is suitable for delivery of various drugs and has good application prospects. And the problems that the emulsifying time in the existing self-emulsifying system is relatively long, a toxic nonionic surfactant and a toxic cosolvent need to be used and the like are also solved.

The utility model relates to the technical field of chromatographic separation devices, and discloses a cholic acid chromatographic separation device which comprises a supporting plate, an anti-skid cushion block is fixedly connected to the lower surface of the supporting plate, a fixing rod is fixedly connected to the interior of the supporting plate, a sliding rod is connected to the outer wall of the fixing rod in a sliding mode, a plurality of second grooves are formed in the sliding rod, and a plurality of second grooves are formed in the second grooves. An adjusting ring is fixedly connected to the outer wall of the sliding rod, a hinge seat is fixedly connected to the outer wall of the adjusting ring, a rotating rod is rotatably connected to the interior of the hinge seat, a spring clamping block is arranged in the rotating rod, a plug pin is slidably connected to the interior of the adjusting ring, and the outer wall of the plug pin is slidably connected to the inner wall of one second groove. According to the utility model, the sliding rod slides on the fixed rod, the slotted hole in the adjusting ring is aligned with the groove in the proper position and is fixed by the bolt, and the spring clamping block connected with the rotating rod is clamped in the clamping groove, so that the sliding rod is supported and assisted in further adjustment, the operation difficulty is reduced, and the working efficiency is improved.

The invention belongs to the technical field of biomedicine synthesis, and particularly relates to the technical field of chemical synthesis of natural drugs / prodrugs for controlling metabolic abnormalities such as blood sugar and blood fat. The invention provides a preparation method of a cholic acid derivative. The preparation method comprises the following steps: adding benzyl bromide into an alkaline solution containing cholic acid to obtain benzyl cholate; the method comprises the following steps: adding a dichloromethane solution containing acyl chloride into a dichloromethane solution containing benzyl cholate and 4-dimethylaminopyridine under protective gas to obtain a colorless oily product; and introducing hydrogen into the absolute ethyl alcohol in which the colorless oily product is dissolved to obtain the cholic acid derivative. The chemical synthesis of the cholic acid derivative is realized.

The invention belongs to the technical field of biochemical pharmacy, and particularly relates to a compound bezoar composition, decoction piece type compound bezoar and a preparation method of the decoction piece type compound bezoar. The compound calculus bovis composition provided by the invention comprises 94-96% by mass of main agents and 8 kinds of calculus bovis intermediates, the mass content of the auxiliary agent is 4-6%, and the auxiliary agent is six commercially available finished product combined cholic acid. The bilirubin content of the bezoar intermediate used in the invention is increased by more than 30% and the yield is increased by more than 25% compared with the existing biochemical reaction process of oxgall. The DPPH free radical scavenging rate of the compound bezoar composition reaches 89.6% + / -2.1% and is increased by 25.7-36.9% compared with 58.3% + / -3.5% of a product prepared from ox bile, and through t inspection, p is smaller than 0.01 (n is equal to 5). Free cholic acid intervention is strictly prohibited, lithocholic acid addition is completely eradicated, and the composition has the advantages of being high in bilirubin content, high in oxidation resistance and high in biological activity and adapts to the physiological characteristics of the human body.

The invention belongs to the technical field of separation equipment, and particularly relates to separation equipment of a cholic acid extraction reaction kettle and a separation method thereof, the separation equipment comprises a kettle base assembly, a separation assembly and a stirring assembly; the kettle base assembly is of an open structure, a kettle cover is clamped to the top of the kettle base assembly, a servo motor is embedded in the top of the kettle cover, and the separation assembly is slidably connected to the inner wall of the kettle base assembly; the stirring assembly is driven by the output end of the servo motor to rotate, so that the stirring assembly stirs raw materials for cholic acid extraction reaction in the separation assembly, in the process that the separation assembly slides along the inner wall of the kettle base assembly, the separation assembly and the stirring assembly are separated, and the stirring assembly is switched to a cholic acid screening state; the stirring assembly is continuously driven by the servo motor to rotate, solid or liquid of cholic acid in the separation assembly is separated, and the stirring or separation state switching use efficiency of the reaction kettle is improved.

The invention belongs to the technical field of fish and shrimp breeding feed additives, and particularly relates to a bile acid composition for fish and shrimp feed and a preparation method of the bile acid composition, and the composition comprises the following components in parts by weight: 50-60 parts of hyocholic acid, 40-55 parts of chenodeoxycholic acid, 40-60 parts of hyodeoxycholic acid, 30-45 parts of methionine glycine dipeptide and 60-115 parts of a compound plant extract. According to the invention, the methionine glycine dipeptide is used for improving the lipid metabolism capability of fishes and shrimps; the composite plant extract is used for enhancing the immunity and the anti-stress capability of the fish and the shrimps.

The application discloses a kind of 3-(cholic acid pentanoyl amide) cyclobutane-1,1-dicarboxylic acid purification method, belong to the technical field of pharmaceutical chemistry intermediate purification.The method includes: (1) its precursor diethyl ester is hydrolyzed in methanol and / or acetonitrile, concentrated after dissolving with water, with organic solvent extraction is discarded organic phase, water phase is adjusted to 2-4 to pH, again with organic solvent extraction, concentrated and obtained crude product;(2) the crude product is dissolved in alcohol solvent, after filtration, ester solvent is added to crystallize, alcohol ester volume ratio 1:2-1:10, filtration, washing, drying.The application removes unreacted ester, monocarboxylic acid byproduct and cholic acid analogue amide and other impurities by alkaline hydrolysis combined with "alkali dissolving acid extracting" and "alcohol dissolving-ester crystallizing" recrystallization.The product obtained by the method has HPLC purity ≥98.2%, yield ≥80%, and the process is stable, simple to operate, suitable for large-scale production.

An esketamine liquid preparation. The esketamine liquid preparation is an aqueous solution agent, comprising esketamine or a pharmaceutically acceptable salt thereof, cholic acid or a derivative thereof, and water, with a pH value of 2.5-5.7. The esketamine liquid preparation is administrated via oral mucosa, and the preparation is high in stability, fast in absorption, high in bioavailability, simple in administration mode, and good in compliance. The esketamine liquid preparation can be used for preventing, relieving, or treating major depression, unipolar depression, treatment-refractory depression, intractable depression, agitated depression, and bipolar depression.

The application provides a paclitaxel molecular umbrella delivery system prodrug, a preparation method and application thereof. The paclitaxel molecular umbrella delivery system prodrug comprises a molecular umbrella target head part composed of two surface amphiphilic molecules connected by spermidine, a paclitaxel drug part, and a connecting group connecting the molecular umbrella target head part and the paclitaxel drug part through a disulfide bond; wherein the surface amphiphilic molecule is selected from cholic acid, deoxycholic acid or a sodium sulfonate thereof. Compared with paclitaxel, the paclitaxel molecular umbrella delivery system prodrug has significantly improved water solubility, lower toxicity to normal cells than free paclitaxel, and significantly improved safety; and the paclitaxel molecular umbrella delivery system prodrug has the following characteristics in a physiological environment: releasing active drugs in the high GSH / ROS microenvironment of tumor cells; and maintaining a prodrug state in normal cells.

This application discloses a lipid control solution, comprising a buffer solution, cholesterol or cholesterol and glycerol, cholic acid or deoxycholic acid and its salts, and cyclodextrin. The lipid control solution provided in this application uses cholic acid or deoxycholic acid and its salts and cyclodextrin as solubilizers to form a complex, allowing cholesterol to dissolve in a water-soluble matrix. It is prepared by direct stirring at room temperature, without the involvement of any organic flammable or harmful reagents. The process is simple, safe, reliable, and exhibits good stability with a shelf life of up to 12 months.

The compositions and methods provided herein are related, in part, to the discovery of cholic acid 7-sulfate as a treatment for diabetes. Provided herein is a method for treating a metabolic disorder (e.g., diabetes, obesity), or an inflammatory disease (e.g., Crohn's disease, inflammatory bowel disease, ulcerative colitis, pancreatitis, hepatitis, appendicitis, gastritis, diverticulitis, celiac disease, food intolerance, enteritis, ulcer, gastroesophageal reflux disease (GERD), psoriatic arthritis, psoriasis, and rheumatoid arthritis) in a subject in need thereof comprising administering to a subject a compound of Formulae (I)-(XVII).

This invention discloses an evaluation method for bile acids in alleviating intestinal inflammation in grouper, belonging to the field of aquaculture technology. Addressing the intestinal inflammation problem caused by feed oxidation, pathogen infection, or water quality deterioration in intensive grouper farming, this invention proposes to achieve efficient regulation of intestinal health by adding a compound bile acid composition to the feed. The core technical solution of the method is as follows: a compound bile acid is formed by compounding porcine deoxycholic acid, chenodeoxycholic acid, and porcine cholic acid in a specific ratio, combined with a carrier premixing process to prepare a bile acid premix, which is then added to the basal feed at 0.15%-0.60% of the total feed mass. This invention effectively alleviates enteritis symptoms in grouper by enhancing the integrity of the intestinal epithelial structure, repairing damaged mucosal barriers, regulating inflammatory responses, and optimizing intestinal flora composition. It provides a safe and efficient technical solution for replacing feed antibiotics in aquaculture, with significant economic and ecological benefits.

The invention provides application of ergosterol in preparation of a medicine for treating pulmonary arterial hypertension. The preparation method of the ergosterol comprises the following steps: cleaning, crushing and drying silkworm chrysalis cordyceps hainanensis sporocarp to obtain cordyceps militaris powder; the method comprises the following steps: mixing cholic acid, deoxycholic acid, methoxypolyethylene glycol and absolute ethyl alcohol to obtain ethanol mother liquor of a supramolecular solvent; carrying out gradient synergistic extraction on the cordyceps militaris extract; carrying out reduced pressure distillation and drying to obtain an extract, and carrying out chromatographic separation and drying; and recrystallizing, cleaning and drying to obtain the product. According to the invention, CO2 expanded ethanol liquid is adopted as a solvent, a cholic acid-deoxycholic acid-methoxypolyethylene glycol supramolecular solvent system technology and homologous adaptability of steroid nucleus structures of etocholic acid and deoxycholic acid and ergosterol are utilized to construct a multi-stage cavity recognition network, and C5-C6 double bonds and C22-C23 double bonds of ergosterol are specifically combined, so that the detection sensitivity of ergosterol is improved, and the detection sensitivity of ergosterol is improved. Meanwhile, the pore diameter of the cavity is regulated and controlled through methoxypolyethylene glycol, and the content of ergosterol in the extract is increased.

The invention belongs to the field of medicine, and provides an aprepitant micelle composition and a preparation method thereof, the aprepitant micelle composition is composed of an active component aprepitant and auxiliary materials such as phospholipid, cholic acid or a salt thereof, a nano-micelle stabilizer, a pH regulator and the like, the dosage form is a freeze-dried powder injection, and the solubility of the aprepitant is remarkably improved; the combination of the nano-micelle stabilizer can significantly improve the stability of the preparation, avoids the use of a large amount of soybean oil for solubilization, and reduces the risk of hyperlipidemia. Ethanol is not contained, so that ethanol anaphylaxis is avoided, and the safety of clinical medication can be remarkably improved. After the freeze-dried powder injection is redissolved, a hot-pressing sterilization mode can be adopted, so that the sterile guarantee level is remarkably improved, and the clinical medication risk is reduced. The aprepitant micelle composition is prepared by adopting a tert-butyl alcohol-water freeze-drying method, the process is simple and convenient, large-scale production is easy, a common freeze-dried powder injection production line can be adopted, special equipment such as a high-pressure homogenizer and a special fat emulsion production line are not needed, and the production cost can be remarkably reduced.

The present invention relates to the field of medicinal chemistry. Specifically disclosed is a use of oxime-based cholic acid (CA) analogs as allosteric agonists for TGR5. In the present invention, CA used as a raw material undergoes a series of reactions to obtain CA intermediates each having an oxime at either or both of positions 7 and 12, and a series of modifications are then carried out on positions 24 and the oximes of these intermediates to obtain CA analogs as represented by formula 1, formula 2, or formula 3. The present invention uses a GloSensor cAMP accumulation assay to test the functional activity and allosteric mechanism of all target compounds on TGR5. Pharmacological results have shown that all synthesized target compounds have relatively good agonistic activity on TGR5 and can positively allosterically regulate the functional activity of chenodeoxycholic acid (CDCA) or lithocholic acid (LCA), that is, the target compounds are all positive allosteric modulators or allosteric agonists for TGR5.

The application provides application of ergosterol in preparation of a medicine for treating pulmonary arterial hypertension. A preparation method of the ergosterol is as follows: Cordyceps militaris Haining strain fruiting bodies are taken, washed, crushed, and dried to obtain Cordyceps militaris powder; cholic acid, deoxycholic acid, polyethylene glycol monomethyl ether and anhydrous ethanol are mixed to obtain an ethanol mother liquor of a supramolecular solvent; Cordyceps militaris extraction liquid is gradiently and cooperatively extracted; vacuum distillation, drying, chromatographic separation, drying, recrystallization, washing and drying are sequentially performed, and the ergosterol is obtained. In the application, CO2 expanded ethanol liquid is used as a solvent, and a cholic acid-deoxycholic acid-polyethylene glycol monomethyl ether supramolecular solvent system technology is used. Relying on the homologous adaptability of the steroid nucleus structure of cholic acid and deoxycholic acid and ergosterol, a multi-level cavity recognition network is constructed, the C5-C6 double bond and the C22-C23 double bond sites of ergosterol are specifically combined, and the content of ergosterol in the extract is improved by regulating the cavity aperture through the polyethylene glycol monomethyl ether.

The application belongs to the field of new adjuvants and new dosage forms of pharmaceutical preparations, and particularly relates to a type I and type II immunogenic cell death inducer ion pairing engineered nanoassemblies as well as construction and application thereof. The nanoassemblies are composed of type I ICD inducers, type II ICD inducers, hydrophobic auxiliary counterions and amphiphilic lipid ion pairing. The type I ICD inducer is selected from mitoxantrone, doxorubicin, oxaliplatin or cyclophosphamide; the type II ICD inducer is selected from hypericin; and the hydrophobic auxiliary counterion is selected from cholesteryl sodium sulfate, cholic acid, deoxycholic acid, chenodeoxycholic acid, lithocholic acid, glycolcholic acid, taurocholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, deoxycholic acid lysine derivative, oleanolic acid and ursolic acid. The type I and type II ICD inducers are combined in the application, and have a synergistic tumor treatment effect.

The invention discloses a method for preparing Aramchol through enzymatic synthesis, and belongs to the technical field of organic synthesis. The technical key points of the invention are as follows: firstly, preparing 3beta, 7alpha, 12alpha-trihydroxy-5beta-cholic acid through an enzyme catalyst with specific selectivity; the method comprises the following steps: reacting 3 beta, 7 alpha, 12 alpha-trihydroxy-5 beta-cholic acid with ammonia water to generate 3 beta-amino-7 alpha, 12 alpha-dihydroxy-5 beta-cholic acid; 3 [beta]-amino-7 [alpha], 12 [alpha]-dyhydroxyl-5 [beta]-cholic acid and methanol are subjected to esterification, such that 3 [beta]-amino-7 [alpha], 12 [alpha]-dyhydroxyl-5 [beta]-cholestane-24-acid methyl ester is generated; the preparation method comprises the following steps: carrying out condensation on 3 [beta]-amino-7 [alpha], 12 [alpha]-dyhydroxyl-5 [beta]-cholestane-24-acid methyl ester and arachidic acid to generate 3 [beta]-amino-7 [alpha], 12 [alpha]-dyhydroxyl-5 [beta]-cholestane-24-acid methyl ester, and then hydrolyzing to obtain Aramchol. The method overcomes the defects that in a chemical synthesis method in the prior art, the number of exposed reaction sites of three hydroxyls of 3alpha, 7alpha, 12alpha-trihydroxy-5beta-cholic acid is large, the consumption of sodium azide and arachiyl chloride is large, side reactions are complex, and reproducibility is poor. The method for synthesizing and preparing the Aramchol by using the enzymatic method is simple to operate and convenient in post-treatment, the process yield of the method is as high as 91% or above, and the method is suitable for industrial production.