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Glycated albumin enzymatic detection kit and detection method thereof

A technology for detection kits and detection methods, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as low specificity, long time for detection results, and complicated operation methods.

Inactive Publication Date: 2014-11-26
BEIJING HOMA BIOLOGICAL ENG
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Problems solved by technology

However, the existing composition for measuring glycated protein has low specificity and low sensitivity, and it takes a long time to obtain the test result, the operation method is complicated, and the test result is not accurate and reliable enough.

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  • Glycated albumin enzymatic detection kit and detection method thereof
  • Glycated albumin enzymatic detection kit and detection method thereof
  • Glycated albumin enzymatic detection kit and detection method thereof

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Embodiment Construction

[0026] The purpose of this invention is to establish an accurate and rapid method for the determination of Glycated Albumin (GA), and for the first time in China to develop and produce a commercialized liquid double reagent Glycated Albumin Detection Kit (enzymatic method) for clinical laboratories Automatic biochemical analyzer detection use. The main principle of the present invention is to use protease, fructosyl amino acid oxidase (Fructosyl amino acid oxidase, FAOD) and other hydrolysis and catalytic reactions to measure serum GA content, and at the same time use bromocresol purple colorimetric method to quantify albumin, and obtain the GA percentage by calculation . Its key technologies mainly include FAOD expression, purification and large-scale production; protease screening and activity determination technology; kit stability research (including buffer and stabilizer screening). The key issues to be solved are: the stability of raw material verification (FAOD, protea...

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Abstract

The invention relates to a glycated albumin (GA) enzymatic detection kit, which comprises a GA reagent 1 and a GA reagent 2. The GA reagent 1 comprises tris(hydroxymethyl)aminomethane, aminoantipyrine (4-AAP), protease K, calcium acetate (CaAc2), potassium ferrocyanide trihydrate (K4Fe(CN)6.3H2O), copper acetate (CuAc2) and Cholic acid. The GA reagent 2 includes tris(hydroxymethyl)aminomethane, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt (TOOS), fructosaminase, Triton X-100 and peroxidase. The invention aims to provide the glycated albumin enzymatic detection kit and its detection method with the characteristics of strong specificity, high sensitivity, short time, simple operation mode, and accurate and reliable detection result.

Description

technical field [0001] The invention relates to a glycosylated albumin (GA) enzyme method detection kit and a detection method thereof. Background technique [0002] Glucose in the human body undergoes a non-enzymatic glycosylation reaction with the N-terminus of serum protein, 90% of which is combined with the 189th lysine in the serum protein chain to form a macromolecular ketamine structure, collectively called glycated serum protein , of which more than 90% is glycated albumin. Therefore, glycated albumin (Glycated Albumin, GA) can reflect the overall level of glycated serum protein. In the diagnosis and monitoring of diabetes, glycated protein is of great significance. Blood sugar monitoring is a very important part of the daily diagnosis and treatment of diabetes. Good blood sugar control can effectively delay the occurrence and development of acute and chronic complications of diabetes. In clinical work, it is very important to control the short-term average blood ...

Claims

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Application Information

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IPC IPC(8): C12Q1/37C12Q1/28C12Q1/26
Inventor 鄢盛恺沈林
Owner BEIJING HOMA BIOLOGICAL ENG
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