16 results about "Aconitine" patented technology

The invention relates to a formula and a preparation method of compound desert cistanche granules, the compound desert cistanche granules are prepared by adding flavor on the basis of Qingzheng dialectical record yang-warming soup, the raw materials comprise desert cistanche, bighead atractylodes rhizome, monkshood, Chinese angelica, cynomorium songaricum, fried semen raphani and perillaseed, and the total weight of a single bag is 7g. The production process comprises the key steps of pretreatment of medicinal materials, independent first decoction of radix aconiti carmichaeli for detoxification, extraction of semen raphani and perillaseed volatile oil, inclusion of beta-cyclodextrin, combined decoction and concentration of all the medicinal materials, dry granulation and the like, and the technical effects that granules are instantly dissolved within 45 + / -5 seconds and the transfer rate of crude drugs is 91.2% are achieved by optimizing the proportion of auxiliary materials and process parameters. The quality of the finished product meets the requirements of Chinese Pharmacopoeia, the content of aconitine is less than or equal to 0.02%, the total number of microbial limit aerobic bacteria is less than or equal to 100 CFU / g, the problems that a traditional compound cistanche deserticola preparation is slow in dissolution speed, unstable in toxicity control and non-standard in production process are solved, and the method is suitable for large-scale industrial production.

The invention discloses a method for determining the content of a quality marker of radix aconiti lateralis praeparata (Linjiang tablets) based on a UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) technology, relates to the technical field of sample detection, and in particular relates to development and application of a method for identifying the quality marker of the radix aconiti lateralis praeparata (Linjiang tablets) and a content method. The method comprises the following steps: firstly, identifying quality markers, including benzoyl mesaconitine, benzoyl aconitine, benzoyl hypaconitine, mesaconitine, hypaconitine and aconitine, before and after processing of the Cinnai radix aconiti carmichaeli (Yinjiang tablets); the quality markers comprise benzoyl mesaconitine, benzoyl mesaconitine, benzoyl hypaconitine, benzoyl mesaconitine, benzoyl hypaconitine and aconitine; secondly, a rapid, accurate and reliable multi-component content determination method based on UPLC-QQQ-MS / MS is established, and finally, multiple batches of actual radix aconiti lateralis praeparata (Linjiang tablets) samples are determined. The established method is high in precision, good in stability and excellent in repeatability, the sample adding recovery rate meets the requirement, accurate quantification of the multiple quality markers can be achieved, and powerful support can be provided for quality control and evaluation of the radix aconiti lateralis praeparata (Linjiang tablets).

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a specific chromatogram establishment method and a content determination method of short-pedicel aconite root. The specific chromatogram construction method comprises the following steps: a, preparation of a test solution: taking short-pedicel aconite root to prepare the test solution; b, preparing a reference substance solution, namely adding a solvent into a brachypodum brachypodum heptin reference substance, a brachypodum brachypodum A reference substance and an aconitine reference substance to prepare a mixed reference substance solution; c, detection: respectively and precisely sucking the reference solution and the test solution, injecting into a high performance liquid chromatograph for detection, taking the specific chromatogram of the reference solution as a reference chromatogram, selecting a common peak from the specific chromatogram of the test solution, and constructing the specific chromatogram of the short-pedicel aconite root. The invention also provides a method for determining the content of short-pedicel aconite root. By adopting the detection conditions, the content of the radix aconiti brachypodi medicinal material can be simultaneously detected, the specific chromatogram of the radix aconiti brachypodi medicinal material can be established, the whole detection process is short in time consumption, and the time and the cost are greatly saved.

The present application belongs to the technical field of traditional Chinese medicine quality detection, and particularly relates to a characteristic spectrum construction method and detection method of Shangtong Tincture developed by using a new technology of ultra-high performance liquid chromatograph-diode array detector combined with multiple wavelength segment switching. The present application specifically comprises the following steps: a. Preparation of reference substance solution: taking appropriate reference substances of Bergenin, Herba Artemisiae Scopariae A, Rutin, Cinnamyl Aldehyde, Paeonol and Aconitine, dissolving the reference substances in a solvent to obtain the reference substance solution; b. Preparation of test sample solution: taking Shangtong Tincture, adding an ethanol solution to prepare the test sample solution; c. Detection: injecting the reference substance solution and the test sample solution into an ultra-high performance liquid chromatograph for detection, taking the characteristic spectrum of the reference substance solution as a reference spectrum, selecting common peaks from the characteristic spectrum of the test sample solution to construct the characteristic spectrum of Shangtong Tincture, and determining 13 common peaks; and the present application also provides a rapid and comprehensive detection method of Shangtong Tincture.

This invention belongs to the pharmaceutical field, specifically relating to a composition for enhancing innate immunity and exerting anti-infective effects, and its uses. Specifically, this invention selects ALOX15 enzyme activity inhibitors, including baicalin; compounds that promote ALOX15 transcriptional activity, including aconitine; and compounds that inhibit ALOX15 protein degradation, including forsythoside A / E / I, and combines them in a multi-strategy scientific formulation. Experimental studies have shown that the composition containing ALOX15 enzyme activity inhibitors, ALOX15 transcriptional activity promoters, and ALOX15 protein degradation inhibitors can effectively enhance the body's innate immunity, increase the synthesis and secretion of IFN-β, and exert anti-pathogen infection effects. The composition of this invention can also inhibit the production of pathogen-induced inflammatory factors and has a therapeutic effect on pathogen-induced secondary inflammatory symptoms, showing great promise for clinical application.

This invention discloses a breiaconitine A derivative having analgesic activity, a method for preparing the same, and its use, relating to the field of medicinal chemistry. A breiaconitine A derivative is a compound represented by formula (I), its isomer, its deuterated compound, its solvate, its prodrug, its metabolite, its crystalline form, or its pharmaceutically acceptable salt. The breiaconitine A derivatives provided in this invention have advantages such as high analgesic activity, good anti-inflammatory activity, low toxicity, high therapeutic index, and high water solubility, and can be applied to the preparation of low-toxicity, highly effective, and independent anti-inflammatory analgesics. [Formula 1] TIFF2026511621000134.tif45170 Formula I

This invention relates to the field of traditional Chinese medicine processing technology, and discloses a method for detoxifying Aconitum carmichaelii (Fuzi) for treating various ailments. The method involves sequentially soaking and softening, slicing, steaming once, initial drying, low-temperature fermentation, high-temperature blanching, rinsing with clean water, steaming a second time, and drying the finished product to obtain Aconitum carmichaelii slices. By using double high-heat steaming to destroy the toxic structure of aconitine, combined with low-temperature long-term fermentation to degrade residual toxicity, and supplemented by high-temperature blanching and multiple rinsing with clean water, deep detoxification is achieved. This invention can completely remove the highly toxic aconitine from Aconitum carmichaelii, leaving no toxic residue, while fully preserving the core medicinal activity of Aconitum carmichaelii. The process is stable and controllable, and the resulting slices have high safety for medicinal use and can be used alone or in combination for the treatment of various ailments.

The application discloses a method for determining the content of aconite alkaloids in a traditional Chinese medicine composition containing Ramulus Cinnamomi, which comprises the following steps: detecting test sample solution and control sample solution of the traditional Chinese medicine composition, and obtaining the content information of the control sample of the traditional Chinese medicine composition according to the detection result; wherein the traditional Chinese medicine composition comprises Ramulus Cinnamomi, Radix Paeoniae Alba, Radix Glycyrrhizae, Ephedra, Rhizoma Zingiberis Recens, Rhizoma Atractylodis Macrocephalae, Rhizoma Anemarrhenae, Radix Saposhnikoviae and Heishunpian, and the control sample is benzoyl neoorientaline, benzoyl orientaline, benzoyl hypoorientaline, neoorientaline, hypoorientaline and aconitine. The application carries out the content monitoring research on the monoester type alkaloids of Heishunpian medicinal flavor in the reference sample of the traditional Chinese medicine composition containing Ramulus Cinnamomi, and carries out the limited control research on the diester type alkaloids, and the research on the reference sample of the traditional Chinese medicine composition containing Ramulus Cinnamomi is carried out from the multiple dimensions of safety and effectiveness, and has important quality control significance.

The invention belongs to the field of drug detection and quality evaluation, and provides a papaya pill adulteration identification method. According to the papaya pill adulteration identification method, the content ratio of benzoyl mesaconitine to benzoyl hypaconitine is used as a prepared radix aconiti agrestis adulteration evaluation standard, and the content ratio of benzoyl mesaconitine to benzoyl aconitine is used as a prepared radix aconiti agrestis adulteration evaluation standard. A large number of experiments accumulate and analyze that the ratio of benzoyl mesaconitine to benzoyl aconitine to benzoyl hypaconitine in radix aconiti preparata, radix aconiti kusnezoffii preparata and related counterfeit products has regular difference, and through statistical analysis, the characteristic ratio capable of being used for counterfeit product identification is found out. Therefore, whether counterfeit feeding exists in the Chinese patent medicine preparation or not is judged, and the problem of true and false feeding of the radix aconiti preparata and the radix aconiti kusnezoffii preparata in the papaya pill preparation is solved.

The invention relates to the technical field of quality analysis of Chinese patent medicines, in particular to an establishment method and content determination method of HPLC characteristic chromatograms of related components of bone-strengthening musk analgesic ointment, and the establishment method comprises the following steps: step 1) preparation of a test solution; taking a bone-strengthening musk analgesic paste sample, pre-treating to remove a volatile matrix, adding a solvent, carrying out ultrasonic extraction, fixing the volume, filtering, and taking a subsequent filtrate to obtain a sample solution; 2) preparing a mixed reference substance solution; taking reference substances of aconitine, 4-methoxysalicylaldehyde, cinnamaldehyde, white angelica brain, gingerol, imperatorin and 6-shogaol, and adding methanol to a constant volume to obtain a reference substance stock solution; weighing the reference substance stock solution, and adding methanol to dilute the reference substance stock solution into a mixed reference substance solution; and (3) respectively measuring the sample solution in the step (1) and the mixed reference substance solution in the step (2) by adopting a high performance liquid chromatography, determining a common peak according to the relative retention time of a reference substance, determining a characteristic peak by utilizing a standard substance, and establishing the HPLC characteristic chromatogram of the bone-strengthening musk analgesic paste. The method has the advantages that the precision, the stability and the repeatability are good, the sample adding recovery rate meets the requirement, and the method can be used for actual quality control of the product.

The invention provides application of mesaconitine in a medicine for treating chronic inflammatory pain and an extraction method of the mesaconitine, and belongs to the technical field of mesaconitine extraction. An acid alcohol-alkali alcohol gradient elution method is adopted for elution and impurity removal, the obtained extract is a mesaconitine monomer component, then column chromatography refining and purification are carried out, mesaconitine with the purity being 95% or above is obtained, the extracted component is single, the preparation quality can be accurately controlled, and pharmacokinetic research and reasonable medication are facilitated. And the new aconitine has a strong effect and provides a new target spot for analgesia.

The invention relates to a method suitable for detecting three aconitine in various compound traditional Chinese medicine preparations, which comprises the following steps: preparing a reference extract solution and a test solution, introducing a magnetic solid-phase extraction technology when the test solution is prepared, and accurately'capturing 'and separating target aconitine from a complex chemical component background; a multi-reaction monitoring mode of triple quadrupole mass spectrometry is adopted, and a Shim-pack Scept C18-120 chromatographic column is adopted as a chromatographic column; the column temperature is 40 DEG C; a mobile phase is 0.14% formic acid aqueous solution (A)-acetonitrile (B), gradient elution is carried out for 0-5 min, 95% A > 5% A; the flow rate is 0.3 mL * min <-1 >; the sample size is 1 microliter; a mass spectrum ion source is an electrospray ionization source, a positive ion multi-reaction monitoring mode is adopted, the capillary voltage is 3.5 kV, the ion source temperature is 150 DEG C, and the solvent removal temperature is 500 DEG C. The high-efficiency purification of MCX and the high-specificity detection of LC-MS / MS are combined to form a set of powerful method system, the method system is successfully applied to various dosage forms with different formulas, and the industry dilemma of'one preparation and one method 'is solved.

The invention discloses a preparation method of an external traditional Chinese medicine liquid for treating synovitis osteoarthrosis, and relates to the technical field of external traditional Chinese medicine preparations, and the preparation method comprises the following steps: raw material pretreatment: taking zaocys dhumnade, radix aconiti preparata, radix aconiti kusnezoffii preparata, radix angelicae pubescentis, rhizoma pinelliae preparata, eucommia ulmoides, radix achyranthis bidentatae, salvia miltiorrhiza, safflower carthamus, lumbricus, centipede, herba portulacae, lycopodium clavatum, phryma leptostachya, semen strychni preparata, radix et rhizoma rhei preparata, borneol and mint; according to the formula disclosed by the invention, aiming at the core pathogenesis of wind-cold-dampness arthralgia and blood stasis blocking collaterals, insect medicines capable of strongly activating meridians to stop pain and medicinal materials such as aconite and semen strychni capable of expelling wind and removing cold are selected, and blood activating and transdermal components are combined, so that the pain can be quickly relieved, the joint function can be improved, and the curative effect is good. The radix aconiti, the radix aconiti agrestis, the semen strychni and the rhizoma pinelliae in the formula are processed products (processed), toxic components (such as aconitine and strychnine) of the radix aconiti, the radix aconiti agrestis, the semen strychni and the rhizoma pinelliae are partially converted or reduced in the processing process, and the medication safety is greatly improved on the premise of strictly controlling the external dosage and range.

The invention belongs to the technical field of network toxicology and metabonomics detection, and provides an aconitine poisoning biomarker as well as a screening method and application thereof, the aconitine poisoning biomarker is phenylalanine, tyrosine and beta-phenylethylamine, specifically, the ratio of phenylalanine to tyrosine is increased, and the ratio of tyrosine to beta-phenylethylamine is decreased. According to the invention, the relative change of increase of phenylalanine / tyrosine ratio and decrease of tyrosine / beta-phenylethylamine ratio is simultaneously used as a judgment standard of related evidence of poisoning caused by taking the aconitum traditional Chinese medicine before life, so that the influence caused by individual difference is greatly reduced; the method disclosed by the invention is suitable for most organic toxic drugs with multi-path and multi-target toxic effects, particularly has a great development prospect in research on poisoning of natural drugs and toxicants, and can be used for rapidly and accurately determining poisoning biomarkers with lower cost through network toxicology and molecular docking technologies; the reference and the application value can be well provided for the prenatal poisoning judgment of the natural medicine poison.

The invention discloses a quality control method for a ginseng-radix aconiti lateralis praeparata injection based on toxicity-effective concentration double detection. The quality control method comprises the following steps: (1) detecting the total amount of aconitine toxic components and the total amount of ginsenoside medicinal components in a ginseng-radix aconiti lateralis praeparata injection test sample; (2) calculating the toxicity-effect concentration ratio, namely the ratio of the total amount of aconitine toxic components to the total amount of ginsenoside medicinal components; and (3) if the total amount of aconitine toxic components is less than or equal to 0.01 mg / mL, the total amount of ginsenoside medicinal components is more than or equal to 0.15 mg / mL and the toxicity-effect concentration ratio is less than or equal to 1: 15, judging that the quality of the ginseng-monkshood injection is qualified. A'toxicity-effect concentration 'dual-detection method is established for the first time, and the core of the method is as follows: synchronous detection of toxic components and medicinal components is realized, and triple control standards of'the upper limit of the total amount of the toxic components, the lower limit of the total amount of the medicinal components and the upper limit of the toxicity-effect concentration ratio' are set, so that the toxicity-effect balance of the preparation is ensured.

The application discloses a kind of aconite alkaloids liquid chromatography analysis method based on ionic liquid technology, belong to pharmaceutical analysis detection technical field.The method of the present application is prepared with aconite medicinal material to prepare test solution, while preparing aconite alkaloids mixed control solution, using high performance liquid chromatography to carry out analysis, wherein ionic liquid is used as mobile phase additive.The method of the present application not only has good separation effect, the obtained chromatographic peak tailing factor tends to 1, is more sharp and has good symmetry, so that benzoyl neowuheadine, benzoyl aconine, benzoyl hypaconine, neowuheadine, hypaconine and aconine these six kinds of aconite alkaloids can be simultaneously detected and analyzed efficiently and accurately.In addition, the present application effectively shortens retention time, saves analysis time, and causes less damage to chromatographic column, and is more suitable for industrial application.