210 results about "Aconitine" patented technology

The present invention provides one medicine composition for treating coronary heat disease ventricular premature beat based on the Chinese medicine theory of benefiting vital energy, nourishing Yin, promoting blood circulation, dredging meridian, clearing away heat-evil in the pericardium and tranquilizing. The medicine composition has 12 kinds of Chinese medicinal materials including ginseng, ophiopogon root, dogwood, red sage, common jujube seed, etc. The pharmacological test shows that the composite medicine capsule has obvious effect of preventing mouse's arrhythmia caused by CaCl2 and decreasing the sustained time of rat's arrhythmia caused by aconitine.

The invention discloses a method for detecting quality of alkaloids in a Tibetan medicine herba aconiti tangutici and preparations thereof. The method comprises the steps of identification, check and content determination, wherein thin layer chromatography is adopted for identification and is characterized by taking a herba aconiti tangutici control medicine as the control and normal hexane-ethyl acetate-methanol-ammonia water as a developer; high performance liquid chromatography is adopted for check and is characterized by taking a mixed solution of an aconitine control substance, a hypaconitine control substance and a new aconitine control substance as the control, acetonitrile-tetrahydrofuran as a mobile phase A and an ammonium acetate water solution with glacial acetic acid as a mobile phase B to carry out gradient elution; high performance liquid chromatography is adopted for hordenine content determination and is characterized by taking a hordenine control substance as the control and an acetonitrile-potassium dihydrogen phosphate water solution as a mobile phase; and back titration is adopted for total alkaloid content determination. The method is stable and reliable, has strong specificity, can ensure the safety, effectiveness and quality controllability of the alkaloids in the Tibetan medicine herba aconiti tangutici and the preparations thereof, and has higher scientificity and application values.

The invention relates to a method for establishing fingerprint of alkaloid in traditional Chinese medicine aconite or extract thereof. The method adopts an opposite phase high performance liquid chromatography, uses octadecylsilane chemically bonded silica or octadsilane chemically bonded silica as a filling agent, and uses an organic phase-buffer solution as a mobile phase, wherein pH value of the organic phase-buffer solution is 3.0 to 8.0, detection wavelength is 230 to 240 nanometers, the organic phase accounts for 20 to 70 percent of volume percentage of the mobile phase, and the organic phase is acetonitrile or methanol; the buffer solution is one of ammonium acetate-acetic acid buffer solution, triethylamine-acetic acid buffer solution, triethylamine-ammonium acetate-acetic acid buffer solution and triethylamine-acetic acid ammonium-acetic acid buffer solution; and the method comprises the following steps: using proper amount of aconitine, mesaconine or hypaconitine to prepare reference substance solution, preparing test solution, and pouring the reference substance solution and the test solution into a high performance liquid chromatograph respectively by accurately sucking to establish the fingerprint of the alkaloid in the traditional Chinese medicine aconite or the extract thereof.

The present invention provides a composition of transdermally administered aconitine alkaloids for ameliorating pain and inflammation. In one aspect, an aconitine alkaloid is delivered in a sufficient amount to achieve and maintian a blood plasma aconitine alkaloid level of about 0.5 ng/mL to about 400 ng/mL. Aconitine alkaloids may be delivered by themselves, or in combination with other elements, such as additional analgesics, other drugs, or positive health promoting substances. Various formulations for the transdermal delivery of aconitine alkaloids are disclosed, and may include selected penetration enhancers.

The invention relates to a preparation and quality control method of a Shaolin rheumatism trauma paste. The original production process of the Shaolin rheumatism trauma paste has the problems that (1) power consumption of plastication is great; (2) temperature rises quickly in the plastication, which results in easy aging and loss of cohesive force; (3) the menthol, menthyl salicylate and borneol in the prescription are once tried for soaking rubber to carry out the plastication, which leads to excessive loss of the components in finished products; when petrolatum is used for replacing the components to soak the rubber for carrying out the plastication, serious problem of oil permeation occurs in the finished products; (4) the relative density of the extract of medical material specified by the standards can not be controlled. The invention aims at providing the preparation and quality control method of the Shaolin rheumatism trauma paste, proposing a reasonable prescription of base materials and a production technique to ensure that finished products have clean and smooth appearance and good cohesive force and reducing the loss of active components of the finished products, particularly the volatile components such as menthol, menthyl salicylate and borneol. The quality standards essentially comprises thin layer identification of angelica, forsythia fruit, rhubarb root, root of common peony and resina draconis, limit tests on adhesion ability and aconitine and determination of the content of menthol, menthyl salicylate and borneol of the products by gas chromatography.

The invention relates to preparation and detecting methods of a traditional Chinese medicine composition for treating wind-cold-wetness evil, belonging to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition is prepared from zaocys dhumnades, schizonepeta, radix saposhnikoviae, prepared rhizoma cyperi with vinegar, radix angelicae pubescentis, ground beeltle, radix clematidis, cassia twig, notopterygium roots, earthworms, radix aconiti preparata and radix aconiti kusnezoffii preparata. The traditional Chinese medicine composition is prepared into easily swallowed pills by using a plastic molding and extensive preparation combined application technology, so that defects of the traditional Chinese medicine composition in clinical application are overcome. The detection for the composition is strengthened through the additional increment of thin-layer chromatography identification, meanwhile, the problem that spots on the limited amount of reference substances of aconitine as a toxic ingredient disappear instantly or do not appear is solved by using a thin layer chromatography, and a method for measuring 5-O-methylvisammioside as a main ingredient is improved by using a high-performance liquid chromatography, so that the technical problems that the content is not up to the standard under the condition that the quailed raw materials are put into production and the transfer rate of effective ingredients is relatively low are solved. The detecting method provided by the invention not only is suitable for industrial production, but also provides a safe and effective detecting means for clinical medication.

The invention discloses a quality inspection method of a traditional Chinese medicine composition twenty-five-ingredient lung disease capsule and a preparation thereof. On the basis of the primary standard, high-performance liquid chromatography (HPLC) is additionally used to perform limit test to aconitine in the twenty-five-ingredient lung disease capsule, and simultaneously the high-performance liquid chromatography (HPLC) is adopted to perform quantitative detection to active ingredients of hydroxysafflor yellow A, swertiamarin and cholic acid, as well as differentiation to fructus terminaliae billericae, sandalwood, kummel, Baxiaga and licorice root. The method improves the product quality, and ensures that the preparation can guarantee the drug use safety and the effectiveness while curing diseases.

The invention relates to a biological pesticide composition. The biological pesticide composition comprises a component A, a component B and a component C, wherein the component A comprises camphor, rhubarb, fennel, pepper and accessories; the component B comprises sophocarpidine, nicotine, aconitine, lavender essential oil, patchouli oil and perilla leaf oil; the component C comprises rhizoma atractylodis, tuckahoe, raw pinellia ternata, pericarpium citri reticulatae and radix angelicae; the weight ratio of the component A to the component B to the component C is 1 to (1.2-2) to ( 2.5-3). The biological pesticide composition has a synergistic interaction effect due to the combination of multiple components, belongs to a compatible pesticide for killing pests, mites and bacteria and can be applied to disease and pest prevention and control for vegetables, fruit trees, flowers and the like; the biological pesticide composition is harmless to crops, has no residue, cannot pollute the environment and agricultural products, cannot kill natural enemies and has no teratogenic, carcinogenic and mutagenic harms to mammals, has a strong pest killing effect and a good prevention effect and is efficient and safe.

The invention relates to a detecting method for an eighteen-component codonopsis pilosula preparation, which comprises one or more of the following identification and/or detection methods: identifying the morphological characteristics of medical materials such as Tibetan codonopsis pilosula, Bangna (a traditional Chinese medicament), cassia seed, Tibetan drug sweetflag rhizome, Chinese tinospora stem, denucleated medicament terminalia fruit, gymnadenia conopsea, fructus terminaliae billericae, frankincense, Kukuinabu (a traditional Chinese medicament) and Baxiaga (a Traditional Tibetan medicament containing vasicine) in the preparation through a microscope; qualitatively identifying costustoot, the Tibetan drug sweetflag rhizome and the frankincense in the preparation through thin layer chromatography; and detecting the content of aconitine in the preparation. By the method, the eighteen-component codonopsis pilosula preparation can be effectively detected.

The invention provides analgesic and antiphlogistic medicines and a micro-needle transdermal administration method for the same. Analgesic and antiphlogistic traditional Chinese medicines with aconitum alkaloid as a representative have the characteristics of long action and non-frequent administration; a micro-needle administration system enables slow release and sustained release of aconitum alkaloid to be realized. A plasma concentration in a range of 0.5 to 500 ng/ml is achieved in 0.5 to 14 h after administration. The invention provides a micro-needle transdermal administration method and system for analgesic and antiphlogistic medicines capable of enhancing pain or inflammation, with aconitum alkaloid included. According to the invention, usage amount of aconitum alkaloid is sufficient to enable a plasma concentration of about 0.5 to 400 ng/ml to be achieved and maintained; administration through skin pricking enables painless administration to be realized, and the medicines themselves do not cause direct irritation or damage to skin surface.

A disclosed environment-friendly pesticide containing natural plants is prepared from the following raw materials in parts by weight: 10-15 parts of raw ginger extracting liquid, 37-59 parts of purified water, 3-6 parts of geranium wilfordii, 6-8 parts of artemisia argyi, 4-8 parts of artemisia carvifolia, 5-10 parts of tomato leaves, 5-9 parts of common cnidium fruit, 5-8 parts of rhizoma ligustici oil, 2-7 parts of tripterygium wilfordii, 9-11 parts of stemona sessilifolia, 6-9 parts of aconitine, 8-12 parts of azone, 3-7 parts of brucine, 15-20 parts of matrine, 4-7 parts of nicoteine and 6-9 parts of clove. The provided environment-friendly pesticide containing natural plants has no drug damage to crops, no residue and no pollution to environment and agricultural products, and is strong in insect killing effect, high in safety, good in preventing effect and low in cost.

The invention relates to a method for simultaneously determining 5 alkaloids in Sini agent vegetable drug plasma, which comprises the following steps: (1) taking medicated plasma of a mammal given Sini agent vegetable drugs, adding bulleyaconitine A (interior label), alkalifying with ammonia water, scrolling, adding ethyl acetate for extraction, scrolling, centrifugating, extracting the subnatant with ethyl acetate twice, merging the ethyl acetate layers, blow-drying with nitrogen, and dissolving the residue with a mobile phase; and (2) HPLC-MS-MS (high performance liquid chromatograph-mass spectrometer-mass spectrometer) determination: the mobile phases comprise a phase A acetonitrile-0.1% glacial acetic acid (1:99, v/v) and a phase B acetonitrile-water (99:1, v/v); the mass spectrometer conditions are as follows: electric spray ion source (ESI) and cation detection mode; and the detection ions are as follows: aconitine m/z 646.3->586.3, mesaconine m/z 632.3->572.3, hypaconitine m/z 616.3->556.3, benzoylmesaconine m/z 590.3->558.3, and benzoylaconine m/z 604.3->554.3. The method is suitable for pharmacokinetic research of aconitine, hypaconitine, mesaconine, benzoylmesaconine and benzoylaconine in Sini agent vegetable drugs in a mammal.

The invention discloses a compound biological pesticide and a preparation method thereof. The compound biological pesticide is prepared by components in parts by weight as follows: 0.1 part of lentinan, 1-5 parts of amino acid, 10-20 parts of bupleurum, 10-30 parts of codonopsis pilosula, 10-20 parts of aconitine, 15-20 parts of radix euphorbiae lantu, 15-25 parts of ephedra and 1-5 parts of a suspending aid. Compared with ordinary chemical pesticide in the prior art, the prepared compound biological pesticide has the characteristics of good effect and low toxicity and can be used as an ideal environment-friendly agricultural plant protection drug; meanwhile, two or more pesticides are compounded, so that on one hand, the bactericidal and insecticidal spectrum can be widened, and on the other hand, the pesticide effect can be improved; and more importantly, the problem of drug resistance due to the fact that a single pesticide is used for a long term is solved.

The invention relates to a benzaconine transdermal gel having effects of relieving pain and resisting inflammation. The gel comprises the following components in percentage by weight: 0.1 to 10 percent of benzaconine, 2 to 30 percent of transdermal penetrating agent, 60 to 95 percent of solvent and 1 to 20 percent of gel matrix. The gel is prepared by dissolving the benzoyl aconine transdermal, the transdermal penetrating agent and the solvent in the gel matrix. The benzaconine keeps the biological activities of relieving the pain and resisting the inflammation of aconitine, the toxicity is obviously reduced, medicaments can directly reach lesion sites through transdermal medicament delivery, and the safety is further improved. The benzaconine and the aconitine have basically consistent transdermal penetrating behaviors, can be directly penetrated into skin and have quick response. Animal medicinal effect tests in which the benzaconine is prepared into the gel show that the gel has obvious and exact pharmacological effects of relieving the pain and resisting the inflammation.

The invention relates to the field of prepared kusnezoff monkshood roots which are traditional Chinese medicine decoction pieces, in particular to a processing method for prepared kusnezoff monkshood root medicinal materials. The method includes the following steps that a, unprocessed kusnezoff monkshood root medicinal materials are obtained, cleaned with water and then soaked in water, the mass ratio of the medicinal materials to the water is 1:(6-10), and the water is replaced once every 12 hours; b, HPLC detection is carried out on soaking liquid, according to a detection result, soaking can be finished when the total concentration of aconitine, mesaconitine and hypaconitine in the soaking liquid is 10-20 mg/L, the medicinal materials are taken out, placed in a cooking pot and boiled in the way of adding water, the mass ratio of the medicinal materials to the water is 1:(6-10), and clear water is replaced once every an hour; and c, the medicinal materials are boiled for 2-4 hours, then taken out, dried in the air, sliced, and dried for 4-6 h at the temperature of 65-75 DEG C, and processed kusnezoff monkshood root products, namely the prepared kusnezoff monkshood roots can be obtained. By the adoption of the method provided by the invention, kusnezoff monkshood root medicinal materials different in quality can be processed effectively, prepared kusnezoff monkshood root products meeting the national pharmacopeia standard are obtained, and economic benefits of enterprises are increased while vast patients are benefited.

The invention discloses a preparation method of Chinese traditional medicine aconite root; the preparing process of black prepared lateral root of aconite includes that aconite root is firstly immersed in water solution; then the aconite root is washed by clean water to be vertically cut into slices with the thickness of approximate 0.5 cm; then the slices are steamed completely and rinsed by clean water, then the aconite root slices are dripped and dyed brown through toning solution; then the aconite root slices are steamed until an oil surface is formed and the aconite root slices have luster; finally, the aconite root slices are dried in the sun or dried under low temperature; the preparing process of white prepared lateral root of aconite includes that aconite root is firstly immersed in water solution; then the aconite root is taken out and peeled to be vertically cut into slices with the thickness of approximate 0.3 cm; the slices are steamed completely and rinsed by clean water, the aconite root slices are dripped and steamed until an oil surface is formed; finally, the aconite root slices are dried in sun or dried under low temperature. The preparing method enables the toxic ingredient aconitine in aconite root to hydrolyze sufficiently so that the application of the aconite root is safer; meanwhile, the total content of effective alkaloid in the aconite root products is maintained to be high and the efficacy is improved. Besides, the preparation method of Chinese traditional medicine aconite root has no environmental pollution and is applicable to industrialization production.

The invention provides a method for converting aconite diester-type alkaloids into ester-type alkaloids. The method comprises the following steps: adding an aconite diester-type alkaloids material into methylbenzene, making the methylbenzene submerge the material, adding long-chain fatty acid into the methylbenzene, then adding pyridine into the methylbenzene, and heating and refluxing the mixture to obtain an ester-type alkaloids product. The aconite diester-type alkaloids material is an aconitine standard product, a medium aconitine standard product or a defective aconitine standard product, or dried powder of Chinese medicinal aconite root or radix aconiti agrestis or monkshood. The electric spray mass spectrometry shows that the method converts the virulent aconite diester-type alkaloids into the ester-type alkaloids, and the conversion rate is more than 90 percent; in addition, the method can realize direct use of Chinese medicinal plants as reaction raw materials, and can avoid the early extraction and purification operations of reactants.

The invention relates to an application method for thaliadine in preparing a medicament for treating arrhythmia. The thaliadine is used for preparing the medicament for treating the arrhythmia; and the prepared medicament can obviously resist the arrhythmia of chloroform induced mice, adrenaline induced rabbits, barium chloride induced rats and aconitine induced rats, and has remarkable effect.

The invention relates to a detection method of a Chinese medicinal composition for treating traumatic injuries, and belongs to the technical field of traditional Chinese medicines and natural medicines. On the basis of thin-layer chromatography identification of radix angelicae sinensis, borneol, fructus gardeniae, radix notoginseng and vinegar processed rhizoma corydalis and limit detection of aconitine, thin-layer detection is performed on frankincense and flos chrysanthemi, a high performance liquid chromatography technology is used, and the determination method for the content of the radixnotoginseng taken as a monarch drug of the Chinese medicinal composition is improved; furthermore, the content determination method of cinnabar which is a toxic medicinal material is newly built. Byadopting the detection method of the Chinese medicinal composition for treating the traumatic injuries, disclosed by the invention, the counterfeit phenomenon of one-sided use of a single extract to replace valuable medicinal materials caused by the low control method of the composition is solved, furthermore, the clinical safety and effectiveness are improved, so that the method is not only suitable for the total quality control of the composition in industrial production, but also provides a safe and effective guarantee for clinical medication.

The invention discloses a pharmaceutical composition of ginseng and monkshood, wherein the content of monkshood alkaloid is not lower than 50%. The pharmaceutical composition provided by the invention has the advantages of controllable quality, better stability, and improved pharmacological actions. The monkshood alkaloid extract is prepared through specially treating monkshood, hydrolyzing di-ester aconitine in monkshood so as to convert it into low-toxicity alkaloid. The obtained composition can be used for treating infectious shock, bleeding shock and cardiogenic shock.

The invention relates to a traditional Chinese medicine preparation for treating rheumatism, which is prepared from the following pharmaceutical products: monkshood, ligusticus wallichii franchet, radix paeoniae rubra, cassia twig, ephedra, asarum, notopterygium root, radix angelicae pubescentis, rhizoma cibotii, garden balsam stem, variegated coralbean bark, pepper stem, herb of common forkstamenflower, rhizoma atractylodis, semen coicis, cortex periplocae and liquorice. The preparation method of the traditional Chinese medicine preparation for treating rheumatism comprises the steps of: extracting pharmaceutical volatile oil from the pharmaceutical products except monkshood, dipping the monkshood in water for one hour, then decocting for three hours, adding the residual dregs of the pharmaceutical products subjected to pharmaceutical volatile oil extraction and residual medicine in the monkshood to decoct twice, filtering, standing the filtrate, extracting supernatant for concentrating to be thick paste, and adding the pharmaceutical volatile oil to prepare the preparation, wherein all of the pharmaceutical products can be extracted to be thick paste for preparing various formulations. The preparation method includes a quality control method. The invention effectively removes aconitine in the monkshood and reserves active ingredients in the monkshood by adopting a particular preparation process. In the invention, the traditional Chinese medicine preparation has definite curative effect, high safety and better curative effect on all kinds of rheumatism, treats both principal and secondary aspect of the disease, and is prepared from pure traditional Chinese medicines so as to be convenient, safe and reliable to use.

The invention relates to a biological pesticide composition. The biological pesticide composition comprises an A component, a B component and a C component. The A component comprises camphor, rheum officinale, chili and accessories. The B component comprises matrine, nicotiana alkaloids, aconitine, lavender essential oil, patchouli oil and perilla leaf oil. The C component comprises rhizoma atractylodes, poria cocos, dried pinellia, dried orange peel and dahurian angelica root. The weight ratio of the A component, the B component and the C component is 1:1.2-2:2.5-3. The combination of the plurality of components has a synergistic effect. The composition belongs to an insecticidal acaricidal bactericidal pesticide, and can be used for disease and pest control for vegetables, fruit trees, flowers, and the like. The composition is free of phytotoxicity for crops, free of residue, free of pollution to the environment and agricultural products, capable of not killing natural enemies, free of hazards causing abnormality, cancer and mutation for mammals, high in pest killing effects, efficient, safe and good in control effects.

The invention relates to a method for detecting medicine composition for warming the spleen and tonifying the kidney and releasing turbidity and eliminating stasis. The method includes content determination and identification methods or detection of limited quantity of aconitine. In the content determination method, the high performance liquid chromatography is adopted. The detection method of the invention also includes identification of ginseng, liquorice and rhizoma zingiberis and detection of aconitine. The detection method of the invention features good specificity, high stability, good repeatability and high precision, thus being more suitable for industrialized production and ensuring safe, effective and reliable clinical medication. In addition, the preparation prepared by the alcohol extraction method has better effect.