32 results about "Aconitine" patented technology

The present invention discloses a method for controlling rice brown planthopper based on sustained-release nano-matrine, belonging to the field of nano-pesticides. In the present invention, matrine is loaded into the mesoporous structure of mesoporous silica, and then encapsulated with chitosan to obtain sustained-release nano-matrine. Then, the sustained-release nano-matrine is formulated into a sustained-release nano-matrine solution, which is then applied as a pesticide to the leaves of rice seedlings to control rice brown planthopper. The sustained-release nano-matrine adopted in the present invention can induce rice seedlings to produce the plant defense hormone jasmonic acid precursor 12-oxophytodienoic acid and the brown planthopper-resistant substances luteolin and cis-aconitine. These substances have a targeted toxic effect and can inhibit the reproduction of offspring, thereby better controlling rice brown planthopper.

The invention relates to a formula and a preparation method of compound desert cistanche granules, the compound desert cistanche granules are prepared by adding flavor on the basis of Qingzheng dialectical record yang-warming soup, the raw materials comprise desert cistanche, bighead atractylodes rhizome, monkshood, Chinese angelica, cynomorium songaricum, fried semen raphani and perillaseed, and the total weight of a single bag is 7g. The production process comprises the key steps of pretreatment of medicinal materials, independent first decoction of radix aconiti carmichaeli for detoxification, extraction of semen raphani and perillaseed volatile oil, inclusion of beta-cyclodextrin, combined decoction and concentration of all the medicinal materials, dry granulation and the like, and the technical effects that granules are instantly dissolved within 45 + / -5 seconds and the transfer rate of crude drugs is 91.2% are achieved by optimizing the proportion of auxiliary materials and process parameters. The quality of the finished product meets the requirements of Chinese Pharmacopoeia, the content of aconitine is less than or equal to 0.02%, the total number of microbial limit aerobic bacteria is less than or equal to 100 CFU / g, the problems that a traditional compound cistanche deserticola preparation is slow in dissolution speed, unstable in toxicity control and non-standard in production process are solved, and the method is suitable for large-scale industrial production.

The invention relates to application of aconitine and a malignant glioma resisting medicine, and relates to the technical field of biological medicine. According to the application, multiple aconitine is applied to intervene in human brain astroblastoma U87-MG cells, and through a CCK-8 cell viability experiment, a Transwell cell migration experiment and a cell apoptosis experiment, it is found that the multiple aconitine can effectively inhibit growth and migration of the human brain astroblastoma U87-MG cells and promote U87-MG cell apoptosis, and then malignant progression of glioma is inhibited; a new medicine is provided for treatment of glioma, and the application prospect is good.

The invention discloses a yang-warming and cold-dispelling health-care liquid and a preparation method thereof. The aconitine content is less than or equal to 0.015 mg / mL and the heat effect lasting time is more than or equal to 8 hours by combining the radix aconiti carmichaeli-rhizoma zingiberis toxicity attenuation synergistic compatibility with the phase change temperature control technology. The transdermal rate of the masticinic acid is increased by 3.5 times by innovatively adopting a micro-jet nanocrystallization process, and the production safety is ensured by matching with near-infrared online monitoring. Animal experiments prove that microcirculation disturbance can be remarkably improved (the blood flow speed is increased by 178%), and the traditional Chinese medicine composition is suitable for local warming and dredging conditioning of people with yang-deficiency constitution.

The invention discloses a tendon-dredging and bone-penetrating health-care liquid and a preparation method thereof. According to the preparation, erycibe obtusifolia benth and radix aconiti preparata are taken as cores, the compatibility design of detoxification of liquorice and synergism of cassia twig is combined with a beta-cyclodextrin inclusion technology, so that the content of aconitine is smaller than or equal to 0.02 mg / mL, and the transdermal rate of ligustrazine is larger than or equal to 65%. The preparation process comprises three modules of dynamic circulation extraction, membrane separation refining and sterile filling, so that the retention rate of volatile components is greater than 85%. Animal experiments prove that the onset time of the analgesic effect is shortened by 40% compared with that of similar products, and the traditional Chinese medicine composition is suitable for conditioning sub-health states such as joint flexion and extension disadvantage.

The invention discloses a method for determining the content of a quality marker of radix aconiti lateralis praeparata (Linjiang tablets) based on a UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) technology, relates to the technical field of sample detection, and in particular relates to development and application of a method for identifying the quality marker of the radix aconiti lateralis praeparata (Linjiang tablets) and a content method. The method comprises the following steps: firstly, identifying quality markers, including benzoyl mesaconitine, benzoyl aconitine, benzoyl hypaconitine, mesaconitine, hypaconitine and aconitine, before and after processing of the Cinnai radix aconiti carmichaeli (Yinjiang tablets); the quality markers comprise benzoyl mesaconitine, benzoyl mesaconitine, benzoyl hypaconitine, benzoyl mesaconitine, benzoyl hypaconitine and aconitine; secondly, a rapid, accurate and reliable multi-component content determination method based on UPLC-QQQ-MS / MS is established, and finally, multiple batches of actual radix aconiti lateralis praeparata (Linjiang tablets) samples are determined. The established method is high in precision, good in stability and excellent in repeatability, the sample adding recovery rate meets the requirement, accurate quantification of the multiple quality markers can be achieved, and powerful support can be provided for quality control and evaluation of the radix aconiti lateralis praeparata (Linjiang tablets).

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a specific chromatogram establishment method and a content determination method of short-pedicel aconite root. The specific chromatogram construction method comprises the following steps: a, preparation of a test solution: taking short-pedicel aconite root to prepare the test solution; b, preparing a reference substance solution, namely adding a solvent into a brachypodum brachypodum heptin reference substance, a brachypodum brachypodum A reference substance and an aconitine reference substance to prepare a mixed reference substance solution; c, detection: respectively and precisely sucking the reference solution and the test solution, injecting into a high performance liquid chromatograph for detection, taking the specific chromatogram of the reference solution as a reference chromatogram, selecting a common peak from the specific chromatogram of the test solution, and constructing the specific chromatogram of the short-pedicel aconite root. The invention also provides a method for determining the content of short-pedicel aconite root. By adopting the detection conditions, the content of the radix aconiti brachypodi medicinal material can be simultaneously detected, the specific chromatogram of the radix aconiti brachypodi medicinal material can be established, the whole detection process is short in time consumption, and the time and the cost are greatly saved.

The present application belongs to the technical field of traditional Chinese medicine quality detection, and particularly relates to a characteristic spectrum construction method and detection method of Shangtong Tincture developed by using a new technology of ultra-high performance liquid chromatograph-diode array detector combined with multiple wavelength segment switching. The present application specifically comprises the following steps: a. Preparation of reference substance solution: taking appropriate reference substances of Bergenin, Herba Artemisiae Scopariae A, Rutin, Cinnamyl Aldehyde, Paeonol and Aconitine, dissolving the reference substances in a solvent to obtain the reference substance solution; b. Preparation of test sample solution: taking Shangtong Tincture, adding an ethanol solution to prepare the test sample solution; c. Detection: injecting the reference substance solution and the test sample solution into an ultra-high performance liquid chromatograph for detection, taking the characteristic spectrum of the reference substance solution as a reference spectrum, selecting common peaks from the characteristic spectrum of the test sample solution to construct the characteristic spectrum of Shangtong Tincture, and determining 13 common peaks; and the present application also provides a rapid and comprehensive detection method of Shangtong Tincture.

This invention belongs to the pharmaceutical field, specifically relating to a composition for enhancing innate immunity and exerting anti-infective effects, and its uses. Specifically, this invention selects ALOX15 enzyme activity inhibitors, including baicalin; compounds that promote ALOX15 transcriptional activity, including aconitine; and compounds that inhibit ALOX15 protein degradation, including forsythoside A / E / I, and combines them in a multi-strategy scientific formulation. Experimental studies have shown that the composition containing ALOX15 enzyme activity inhibitors, ALOX15 transcriptional activity promoters, and ALOX15 protein degradation inhibitors can effectively enhance the body's innate immunity, increase the synthesis and secretion of IFN-β, and exert anti-pathogen infection effects. The composition of this invention can also inhibit the production of pathogen-induced inflammatory factors and has a therapeutic effect on pathogen-induced secondary inflammatory symptoms, showing great promise for clinical application.

The invention relates to the technical field of antibodies, in particular to a monoclonal antibody generated by an aconitine hybridoma cell strain and application of the monoclonal antibody. The aconitine hybridoma cell strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the aconitine hybridoma cell strain is classified and named as the aconitine hybridoma cell strain, the preservation date is June 5, 2025, and the preservation number is CGMCC No.46541. The preparation method comprises the following steps: preparing an artificially synthesized antigen with immunocompetence and characteristics by taking aconitine as a raw material, inducing a mouse to generate a specific antibody in an immunization manner, and then preparing a hybridoma cell strain capable of specifically recognizing diester-type aconitine (aconitine, hypaconitine and mesaconitine) through cell fusion. The generated monoclonal antibody is successfully applied to products such as an immunoaffinity column, an enzyme-linked immunosorbent assay kit and a colloidal gold immunochromatography detection card.

This invention discloses a breiaconitine A derivative having analgesic activity, a method for preparing the same, and its use, relating to the field of medicinal chemistry. A breiaconitine A derivative is a compound represented by formula (I), its isomer, its deuterated compound, its solvate, its prodrug, its metabolite, its crystalline form, or its pharmaceutically acceptable salt. The breiaconitine A derivatives provided in this invention have advantages such as high analgesic activity, good anti-inflammatory activity, low toxicity, high therapeutic index, and high water solubility, and can be applied to the preparation of low-toxicity, highly effective, and independent anti-inflammatory analgesics. [Formula 1] TIFF2026511621000134.tif45170 Formula I

This invention relates to the field of traditional Chinese medicine processing technology, and discloses a method for detoxifying Aconitum carmichaelii (Fuzi) for treating various ailments. The method involves sequentially soaking and softening, slicing, steaming once, initial drying, low-temperature fermentation, high-temperature blanching, rinsing with clean water, steaming a second time, and drying the finished product to obtain Aconitum carmichaelii slices. By using double high-heat steaming to destroy the toxic structure of aconitine, combined with low-temperature long-term fermentation to degrade residual toxicity, and supplemented by high-temperature blanching and multiple rinsing with clean water, deep detoxification is achieved. This invention can completely remove the highly toxic aconitine from Aconitum carmichaelii, leaving no toxic residue, while fully preserving the core medicinal activity of Aconitum carmichaelii. The process is stable and controllable, and the resulting slices have high safety for medicinal use and can be used alone or in combination for the treatment of various ailments.

The present invention relates to the technical field of extraction and application of traditional Chinese medicine, and in particular to aconitine A and its extraction method and application. 18 The new compound of diterpenoid alkaloids has a good ability to scavenge free radicals. 50 The value is 87.2±2.4μg / mL, which can be used as an alkaline antioxidant and can be used in sunscreen cosmetics and free radical scavenging related drugs.

The application discloses a method for determining the content of aconite alkaloids in a traditional Chinese medicine composition containing Ramulus Cinnamomi, which comprises the following steps: detecting test sample solution and control sample solution of the traditional Chinese medicine composition, and obtaining the content information of the control sample of the traditional Chinese medicine composition according to the detection result; wherein the traditional Chinese medicine composition comprises Ramulus Cinnamomi, Radix Paeoniae Alba, Radix Glycyrrhizae, Ephedra, Rhizoma Zingiberis Recens, Rhizoma Atractylodis Macrocephalae, Rhizoma Anemarrhenae, Radix Saposhnikoviae and Heishunpian, and the control sample is benzoyl neoorientaline, benzoyl orientaline, benzoyl hypoorientaline, neoorientaline, hypoorientaline and aconitine. The application carries out the content monitoring research on the monoester type alkaloids of Heishunpian medicinal flavor in the reference sample of the traditional Chinese medicine composition containing Ramulus Cinnamomi, and carries out the limited control research on the diester type alkaloids, and the research on the reference sample of the traditional Chinese medicine composition containing Ramulus Cinnamomi is carried out from the multiple dimensions of safety and effectiveness, and has important quality control significance.

The invention belongs to the field of drug detection and quality evaluation, and provides a papaya pill adulteration identification method. According to the papaya pill adulteration identification method, the content ratio of benzoyl mesaconitine to benzoyl hypaconitine is used as a prepared radix aconiti agrestis adulteration evaluation standard, and the content ratio of benzoyl mesaconitine to benzoyl aconitine is used as a prepared radix aconiti agrestis adulteration evaluation standard. A large number of experiments accumulate and analyze that the ratio of benzoyl mesaconitine to benzoyl aconitine to benzoyl hypaconitine in radix aconiti preparata, radix aconiti kusnezoffii preparata and related counterfeit products has regular difference, and through statistical analysis, the characteristic ratio capable of being used for counterfeit product identification is found out. Therefore, whether counterfeit feeding exists in the Chinese patent medicine preparation or not is judged, and the problem of true and false feeding of the radix aconiti preparata and the radix aconiti kusnezoffii preparata in the papaya pill preparation is solved.

The invention relates to the technical field of traditional Chinese medicine decoction piece processing, and particularly discloses a processing method of radix aconiti carmichaeli (Linjiang tablets). The invention relates to a traditional processing technology of 'Cinnamomum camphora' characteristic, which solves the problems of unstable toxic components and large quality fluctuation of processed products in the prior art by accurately controlling the soaking and bleaching time sequence, the steaming time length and the drying temperature. Through processing, the mass fraction of the toxic component mesaconitine in the radix aconiti carmichaeli is reduced to be not detected from 0.0300% before processing; the mass fraction of hypaconitine and the mass fraction of aconitine are both reduced from 0.0156% to 0.0064% to below ten thousandth. The mass fraction of the effective component benzoyl mesaconitine is increased to 0.0097% and 0.0013% from 0.0034% and 0.0004% before processing, and the mass fractions of the effective component benzoyl mesaconitine and the benzoyl mesaconitine are respectively increased to 285.97% and 353.59%. According to the processing method, the content of toxic components in the salted radix aconiti lateralis praeparata can be remarkably reduced, the content of effective components can be increased, the effects of reducing toxicity and increasing efficiency are achieved, the safety index of the Chinese pharmacopoeia is met, and the processing method is suitable for standardized production of toxic decoction pieces of radix aconiti lateralis praeparata.

The invention discloses an external traditional Chinese medicine formula for treating child abdominal pain and a preparation method, and belongs to the field of traditional Chinese medicines. Syzygium aromaticum and cortex cinnamomi in a ratio of 1: 2.5 enhance the effects of warming spleen and stomach for dispelling cold, and fennel and fructus evodiae in a ratio of 2: 1 enhance the effects of promoting qi circulation and relieving pain. The innovation points are as follows: radix aconiti carmichaeli is boiled for 2 hours by liquorice and ginger (5: 1: 1) to degrade diester aconitine, and then the diester aconitine and fructus amomi (10: 1) are stir-fried for secondary toxicity reduction, so that the safety is ensured; the medicinal materials are dried at a low temperature of less than or equal to 50 DEG C, are subjected to superfine grinding to 200 meshes and are mixed with rice vinegar in a ratio of 3: 1 to prepare paste, so that the transdermal absorption rate is increased by 30%. During external application, the Shenque acupoint and the Zhongwan acupoint are combined, hot ironing is carried out at 40-45 DEG C for 10 minutes, 4-6 hours are taken every day, and the curative effect is improved by 20% compared with that of a single acupoint. Clinical verifications prove that the total effective rate on complex syndromes such as congealing cold, qi stagnation and deficiency-cold in spleen and stomach is 92%, and the adverse reaction rate of skin is lower than 3%. According to the scheme, through toxicity control, proportion optimization and process standardization, the problems of toxicity residues, unstable pesticide effect and the like of a traditional external treatment method are solved.

The invention relates to the technical field of quality analysis of Chinese patent medicines, in particular to an establishment method and content determination method of HPLC characteristic chromatograms of related components of bone-strengthening musk analgesic ointment, and the establishment method comprises the following steps: step 1) preparation of a test solution; taking a bone-strengthening musk analgesic paste sample, pre-treating to remove a volatile matrix, adding a solvent, carrying out ultrasonic extraction, fixing the volume, filtering, and taking a subsequent filtrate to obtain a sample solution; 2) preparing a mixed reference substance solution; taking reference substances of aconitine, 4-methoxysalicylaldehyde, cinnamaldehyde, white angelica brain, gingerol, imperatorin and 6-shogaol, and adding methanol to a constant volume to obtain a reference substance stock solution; weighing the reference substance stock solution, and adding methanol to dilute the reference substance stock solution into a mixed reference substance solution; and (3) respectively measuring the sample solution in the step (1) and the mixed reference substance solution in the step (2) by adopting a high performance liquid chromatography, determining a common peak according to the relative retention time of a reference substance, determining a characteristic peak by utilizing a standard substance, and establishing the HPLC characteristic chromatogram of the bone-strengthening musk analgesic paste. The method has the advantages that the precision, the stability and the repeatability are good, the sample adding recovery rate meets the requirement, and the method can be used for actual quality control of the product.

The invention provides application of mesaconitine in a medicine for treating chronic inflammatory pain and an extraction method of the mesaconitine, and belongs to the technical field of mesaconitine extraction. An acid alcohol-alkali alcohol gradient elution method is adopted for elution and impurity removal, the obtained extract is a mesaconitine monomer component, then column chromatography refining and purification are carried out, mesaconitine with the purity being 95% or above is obtained, the extracted component is single, the preparation quality can be accurately controlled, and pharmacokinetic research and reasonable medication are facilitated. And the new aconitine has a strong effect and provides a new target spot for analgesia.

The invention relates to a method suitable for detecting three aconitine in various compound traditional Chinese medicine preparations, which comprises the following steps: preparing a reference extract solution and a test solution, introducing a magnetic solid-phase extraction technology when the test solution is prepared, and accurately'capturing 'and separating target aconitine from a complex chemical component background; a multi-reaction monitoring mode of triple quadrupole mass spectrometry is adopted, and a Shim-pack Scept C18-120 chromatographic column is adopted as a chromatographic column; the column temperature is 40 DEG C; a mobile phase is 0.14% formic acid aqueous solution (A)-acetonitrile (B), gradient elution is carried out for 0-5 min, 95% A > 5% A; the flow rate is 0.3 mL * min <-1 >; the sample size is 1 microliter; a mass spectrum ion source is an electrospray ionization source, a positive ion multi-reaction monitoring mode is adopted, the capillary voltage is 3.5 kV, the ion source temperature is 150 DEG C, and the solvent removal temperature is 500 DEG C. The high-efficiency purification of MCX and the high-specificity detection of LC-MS / MS are combined to form a set of powerful method system, the method system is successfully applied to various dosage forms with different formulas, and the industry dilemma of'one preparation and one method 'is solved.

The invention relates to the technical field of medicines, and discloses diterpenoid alkaloid 3-acetyl aconitine as well as a pharmaceutical composition and application of the diterpenoid alkaloid 3-acetyl aconitine. The C19 type diterpenoid alkaloid 3-acetyl aconitine provided by the invention shows remarkable anti-depression activity in a mouse behavioral despair model experiment, is obviously superior to a positive drug fluoxetine, and can be used for developing anti-depression drugs.

The invention discloses an application of disulfiram and / or irisin in preparation of a medicine for treating aconitine poisoning. It is found through a large number of attempts that disulfiram can inhibit abnormal expression of alpha-syn in central nervous system caused by aconitine, irisin can inhibit aggregation of alpha-syn, both disulfiram and irisin can rescue PI3K / Akt / mTOR pathway abnormity caused by aconitine, disulfiram or irisin is applied to dyskinesia caused by aconitine, the remarkable improvement effect can be achieved, and the application range of disulfiram or irisin is widened. And an effective strategy is provided for toxicity reduction treatment of clinical aconitine-containing drug poisoning.

The invention discloses a preparation method of an external traditional Chinese medicine liquid for treating synovitis osteoarthrosis, and relates to the technical field of external traditional Chinese medicine preparations, and the preparation method comprises the following steps: raw material pretreatment: taking zaocys dhumnade, radix aconiti preparata, radix aconiti kusnezoffii preparata, radix angelicae pubescentis, rhizoma pinelliae preparata, eucommia ulmoides, radix achyranthis bidentatae, salvia miltiorrhiza, safflower carthamus, lumbricus, centipede, herba portulacae, lycopodium clavatum, phryma leptostachya, semen strychni preparata, radix et rhizoma rhei preparata, borneol and mint; according to the formula disclosed by the invention, aiming at the core pathogenesis of wind-cold-dampness arthralgia and blood stasis blocking collaterals, insect medicines capable of strongly activating meridians to stop pain and medicinal materials such as aconite and semen strychni capable of expelling wind and removing cold are selected, and blood activating and transdermal components are combined, so that the pain can be quickly relieved, the joint function can be improved, and the curative effect is good. The radix aconiti, the radix aconiti agrestis, the semen strychni and the rhizoma pinelliae in the formula are processed products (processed), toxic components (such as aconitine and strychnine) of the radix aconiti, the radix aconiti agrestis, the semen strychni and the rhizoma pinelliae are partially converted or reduced in the processing process, and the medication safety is greatly improved on the premise of strictly controlling the external dosage and range.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a method for determining the content of alkaloid components in short-pedicel aconite roots through an HPLC-ELSD method. The method comprises the following steps: a, preparation of a test solution: taking short-pedicel aconite root, adding an ethanol solution for extraction, filtering, and taking a subsequent filtrate to obtain the test solution; b, preparation of a reference substance solution: weighing a brachypodum brachypodum B reference substance, a benzoyl aconitine reference substance, a brachypodum brachypodum heptin reference substance, an aconitine reference substance and a brachypodum brachypodum A reference substance, and adding methanol to prepare a mixed reference substance solution; and c, respectively precisely sucking the reference solution and the test solution, injecting into a high performance liquid chromatograph, detecting by adopting an evaporative light-scattering detector, and calculating by adopting an external standard two-point method logarithmic equation. The method disclosed by the invention has good linearity, repeatability, stability and recovery rate, and the quality of the short-pedicel aconite root can be well controlled.

The invention belongs to the technical field of network toxicology and metabonomics detection, and provides an aconitine poisoning biomarker as well as a screening method and application thereof, the aconitine poisoning biomarker is phenylalanine, tyrosine and beta-phenylethylamine, specifically, the ratio of phenylalanine to tyrosine is increased, and the ratio of tyrosine to beta-phenylethylamine is decreased. According to the invention, the relative change of increase of phenylalanine / tyrosine ratio and decrease of tyrosine / beta-phenylethylamine ratio is simultaneously used as a judgment standard of related evidence of poisoning caused by taking the aconitum traditional Chinese medicine before life, so that the influence caused by individual difference is greatly reduced; the method disclosed by the invention is suitable for most organic toxic drugs with multi-path and multi-target toxic effects, particularly has a great development prospect in research on poisoning of natural drugs and toxicants, and can be used for rapidly and accurately determining poisoning biomarkers with lower cost through network toxicology and molecular docking technologies; the reference and the application value can be well provided for the prenatal poisoning judgment of the natural medicine poison.

The invention relates to the technical field of medicine, in particular to a herbaceous plant formula for eliminating dampness, easing pain and stimulating menstrual flow, the herbaceous plant formula comprises 5.0 parts of radix aconiti, 5.0 parts of radix aconiti agrestis, 3.0 parts of asarum, 7.0 parts of unprocessed radix aconiti carmichaeli, 8.0 parts of radix angelicae, 8.0 parts of radix saposhnikoviae, 1.0 part of fructus rosae laevigatae, 12.0 parts of radix aucklandiae, 6.0 parts of rhizoma zingiberis, 1000g of honey and 500g of lotus root starch, the radix aconiti, the radix aconiti agrestis and the unprocessed radix aconiti carmichaeli in the formula are main forces for dispelling wind, eliminating dampness, warming meridians and relieving pain; a mode of changing clear water every 12 hours and lightly stirring the medicinal materials is adopted, so that the volume ratio of the medicinal materials to the water is 1: 5, and most toxins can be'driven 'into the water; the raw materials are steamed for 2 hours with steam of 100 DEG C and aired until the water content is 15%, toxic components can be destroyed, the effects of warming channels, dispelling cold and activating meridians to stop pain can be reserved, through the treatment, the content of aconitine components in the finished product can be reduced by 60%-70%, the drug use risk is greatly reduced while the effects of eliminating dampness, easing pain and stimulating menstrual flow are achieved, and the treatment cost is reduced. And people with long-term external application or sensitive skin can also use the composition at ease.

The invention provides radix aconiti lateralis praeparata slice formula granules and a preparation method thereof, and the preparation method comprises the steps of decocting, extracting, concentrating, drying and granulating radix aconiti lateralis praeparata slices in sequence to obtain the radix aconiti lateralis praeparata slice formula granules. Wherein the decocting and extracting step comprises the following steps of: putting radix aconiti lateralis preparata slices into boiling water in an extracting tank; sequentially carrying out three times of extraction, combining the extracting solutions obtained by each time of extraction, and filtering to obtain radix aconiti lateralis praeparata slice filtrate. The preparation method solves the technical problem of low extraction rate of benzoyl mesaconitine, benzoyl aconitine and benzoyl hypaconitine in the existing preparation method of the radix aconiti lateralis praeparata tablet formula granules.

The invention relates to the technical field of traditional Chinese medicine preparation detection, in particular to a detection method of a traditional Chinese medicine spray, during specific use, firstly, chromatographic conditions are set: a chromatographic column takes octadecylsilane chemically bonded silica as a filler, acetonitrile as a mobile phase A and a 0.2% glacial acetic acid solution as a mobile phase B for gradient elution, and the detection wavelength is 235-280 nm; then taking 20mg of aconite diester alkaloid reference extract marked with the content of mesaconitine, hypaconitine and aconitine, precisely weighing, putting into a 10ml measuring flask, dissolving with a solution, diluting to the scale, and uniformly shaking to obtain a reference extract solution; establishing a standard curve based on the reference extract solution; and sample detection is carried out based on the established standard curve, so that the technical problems of insufficient accuracy and tedious operation in the process of detecting the content of the aconitum diester alkaloid due to the absence of a specific aconitum diester alkaloid content detection mode in the prior art are solved.

The invention discloses a quality control method for a ginseng-radix aconiti lateralis praeparata injection based on toxicity-effective concentration double detection. The quality control method comprises the following steps: (1) detecting the total amount of aconitine toxic components and the total amount of ginsenoside medicinal components in a ginseng-radix aconiti lateralis praeparata injection test sample; (2) calculating the toxicity-effect concentration ratio, namely the ratio of the total amount of aconitine toxic components to the total amount of ginsenoside medicinal components; and (3) if the total amount of aconitine toxic components is less than or equal to 0.01 mg / mL, the total amount of ginsenoside medicinal components is more than or equal to 0.15 mg / mL and the toxicity-effect concentration ratio is less than or equal to 1: 15, judging that the quality of the ginseng-monkshood injection is qualified. A'toxicity-effect concentration 'dual-detection method is established for the first time, and the core of the method is as follows: synchronous detection of toxic components and medicinal components is realized, and triple control standards of'the upper limit of the total amount of the toxic components, the lower limit of the total amount of the medicinal components and the upper limit of the toxicity-effect concentration ratio' are set, so that the toxicity-effect balance of the preparation is ensured.

The invention provides an extraction method of total original alkali rich in mesaconitine and application of the total original alkali in a chronic inflammatory pain medicine, and belongs to the technical field of extraction of the mesaconitine. The method comprises the following steps: firstly, decocting radix aconiti with alkaline water for extraction, then, removing impurities with resin, sequentially adopting water, a NaOH solution, water and a dilute ethanol solution as eluents for elution and impurity removal in the impurity removal process, and then, carrying out acid-alcoholysis adsorption. According to the method, the concentration and dosage of the eluent in the impurity removal and desorption processes are adjusted, the content of the mesaconitine is increased, the extract can be used for preparing the medicine for treating chronic inflammatory pain, and the analgesic effect is improved.