The invention relates to a preparation method of a corn bran dietary fiber fat substitute. The preparation method comprises the following steps: (1), crushing, sieving, extracting and leaching the corn bran; (2), carrying out enzymolysis to on filter residue; (3), carrying out alkalization after the enzymolysis; (4), etherifying after the alkalization to obtain a crude product of the corn bran dietary fiber fat substitute; (5), dissolving the crude product and regulating the pH of the mixture to 7.0-9.0, leaching the mixture, and concentrating the filtrate; and (6), adding alcohol to the concentrated solution to separate out precipitate; and leaching the concentrated solution, drying or vacuum freezing and drying the filter residue at 70-80 DEG C to obtain the corn bran dietary fiber fat substitute. The corn bran dietary fiber fat substitute prepared from by the preparation method disclosed by the invention has functional characteristics of fat in the food and enhances enhanced water holding capacity, and improves improved food texture and various sensory characteristics; and moreover, the material is cheap and easy to obtain, the preparation method is scientific and reasonable, the operation is simple and easy to perform, and no pollution is caused to the environment.