Method for preparing resistant starch of corn
A technology of resistant starch and corn starch, which is applied in food preparation, application, food science, etc., can solve the problems of low yield of resistant starch products and lack of high amylose raw materials, etc., to improve food texture, low water holding capacity, and taste delicate effect
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Embodiment 1
[0019] (1) Add fermentation medium in a 2L Erlenmeyer flask, the medium is added per liter of distilled water, the concentration of starch is 60g / L, the concentration of beef extract is 5g / L, the concentration of diammonium sulfate is 5.0g / L, and the concentration of potassium dihydrogen phosphate is 1.00g / L, magnesium sulfate concentration 0.10g / L, initial pH=6, medium volume 1.0L, rotating speed 170rpm, insert pullulanase-producing bacteria Bacillus, inoculum size 7%, and ferment at 36°C for 60h.
[0020] (2) Centrifuge the fermentation liquid obtained in the above steps at 4°C, remove the liquid part, add 300mL of distilled water, use a rubber dropper to absorb bacteria and other impurities, and then wash and separate the starch repeatedly for 4 times, then recover the starch, and store the starch at 40°C Under drying, obtain 56g starch;
[0021] (3) 2 kg of the starch obtained in the above steps was adjusted to a starch milk mass concentration of 35% with distilled water,...
Embodiment 2
[0024] (1) Add fermentation medium in a 2L Erlenmeyer flask, the medium is added per liter of distilled water, the concentration of starch is 60g / L, the concentration of beef extract is 4.5g / L, the concentration of diammonium sulfate is 5.5g / L, and the concentration of potassium dihydrogen phosphate is 1.00 g / L, magnesium sulfate concentration 0.10g / L, initial pH=6, medium volume 0.9L, rotation speed 170rpm, insert pullulanase-producing bacteria Bacillus, inoculum size 6%, ferment at 36°C for 60h .
[0025] (2) Centrifuge the fermentation broth at 4°C, remove the liquid part, add 400mL of distilled water, absorb bacteria and other impurities with a rubber dropper, and then wash and separate the starch repeatedly for 4 times, then recover the starch, and dry the starch at 40°C to obtain 50g fermented starch;
[0026] (3) Get 2kg of the starch obtained in the above steps, adjust the starch milk concentration to 30% with distilled water, pretreat with boiling water for 10 minute...
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