Lactobacillus plantarum with protection function on stroke

A technology of protection of Lactobacillus plantarum, applied in the field of microorganisms, can solve the problems of exacerbation of cell damage and exacerbation of the disease, and achieve excellent anti-oxidation and anti-apoptosis effects

Active Publication Date: 2020-07-10
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, blood reperfusion will aggravate cell damage and lead to aggravation of the disease, that is, ischemia reperfusion injury (I / R)

Method used

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  • Lactobacillus plantarum with protection function on stroke
  • Lactobacillus plantarum with protection function on stroke
  • Lactobacillus plantarum with protection function on stroke

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Rat cerebral ischemia-reperfusion model making and experimental grouping

[0035] Rat cerebral ischemia-reperfusion model establishment: All rats were fasted for 12 hours before operation but water was not allowed. After weighing, 10% chloral hydrate (mass concentration) was injected intraperitoneally for anesthesia (3.5mL / kg), and the supine position was fixed. Make a midline incision, separate the muscle and fascia along the inner border of the sternocleidomastoid muscle, separate the left common carotid artery (CCA), external carotid artery (ECA) and internal carotid artery (ICA), and separate the distal and proximal ends of the CCA Hang up at the end and ECA for standby. Temporarily clamp the ICA with an arteriole clip, then ligate the CCA and ECA at the proximal end, then cut a small opening 4mm from the bifurcation of the CCA, insert the embedment into the ICA, and lightly wrap the thin wire wrapped around the distal end of the CCA. Tighten the tether ...

Embodiment 2

[0038] Example 2: Structural adjustment of Lactobacillus plantarum AR113 to I / R rat tongue coating microbiota

[0039] The effect of L. plantarum AR113 on the tongue coating flora of I / R rats was detected by 16S rDNA high-throughput sequencing technology. The change results of α-diversity index are shown in Table 2. Compared with the sham operation group, the richness and diversity of tongue coating flora of I / R rats showed a downward trend, and the reduction of Chao index and Shannon index was statistically significant ( P<0.05), indicating that cerebral ischemia-reperfusion injury can lead to the migration of the flora structure of the rat tongue coating, and the abundance and diversity of the flora are significantly reduced. However, the intervention of AR113 significantly improved the diversity of the flora. Compared with the I / R group, after the AR113 treatment, the abundance and diversity index of the tongue coating flora in rats had no significant difference, but they a...

Embodiment 3

[0056] Example 3: Structural regulation of the intestinal microbiota of I / R rats by Lactobacillus plantarum AR113

[0057] This example studies the effect of AR113 intake on intestinal flora in I / R rat feces samples. The α-diversity index changes are shown in Table 4. Compared with the sham operation, the richness index (ACE, Chao) and diversity index (Shannon, Simpson) of I / R rat intestinal flora were significantly reduced, indicating that I After / R injury, the structural diversity of intestinal flora in rats decreased, and the intervention of AR113 could significantly improve the reduction of intestinal flora diversity caused by I / R injury, indicating that AR113 can regulate the intestinal flora diversity of I / R rats to a certain extent. Intestinal flora, play a role in improving.

[0058] Table 4 α-diversity index of intestinal flora in rats

[0059]

[0060]

[0061] Note: * P** P*** P# P## P### P<0.001 vs I / R group.

[0062] Image 6 It is a composition diagra...

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Abstract

The invention provides a lactobacillus plantarum AR113 strain with a protection function on stroke. The lactobacillus plantarum AR113 is preserved in the China General Microbiological Culture Collection Center on March 22, 2017, and the preservation number is CGMCCC No.13909. By adopting the lactobacillus plantarum AR113 strain provided by the invention is capable of improving antioxidant enzyme activities of brain tissue, reducing levels of oxidation products, activating Nrf-ARE signal channels, regulating relative expression amounts of antioxidation factors Nrf2, NQO01 and HO-1, meanwhile, reducing miRNA (micro ribonucleic acid) expression of apoptosis-promoting factors Cyt-C, Caspase-3 and Bax, increasing relative expression of an anti-apoptosis factor Bcl-2, and furthermore, relievingbran cell damage caused by cerebral ischemia reperfusion.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a plant lactobacillus which has a protective effect on cerebral apoplexy. Background technique [0002] Stroke is a serious manifestation of multiple cerebrovascular diseases, including hemorrhagic and ischemic strokes. In my country, the incidence of ischemic stroke is much higher than that of hemorrhagic stroke, accounting for about 70% of the total. After the occurrence of ischemic stroke, restoring the cerebral blood flow of patients as soon as possible is one of the most effective medical methods for treating patients. However, blood reperfusion will aggravate cell damage and lead to aggravation of the disease, that is, ischemia reperfusion injury (I / R). Inflammation, oxidative stress, and apoptosis are the main mechanisms of cerebral ischemia-reperfusion injury, and they are also important causes of neurological deficits in post-stroke patients. [0003] As a kind of ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P9/10A61P25/00C12R1/25
CPCA61K35/747A61P9/10A61P25/00C12R2001/25C12N1/205Y02A50/30
Inventor 艾连中熊智强夏永军王光强张汇赖凤羲宋馨
Owner UNIV OF SHANGHAI FOR SCI & TECH
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