The invention discloses a method for evaluating the influence of
gene editing wheat on non-target
organism symbiotic microorganisms, and relates to the technical field of safety evaluation of
gene editing plants on non-target organisms. The method comprises the following steps: selecting non-target organisms growing on a
gene editing wheat material and a
control material at the same time, analyzing
diversity index difference through
DNA extraction, PCR amplification and high-
throughput sequencing, carrying out inter-group
difference analysis by utilizing a difference detection method, and carrying out quantitative analysis and
statistical analysis by adopting a method of combining
qualitative analysis and
statistical analysis. The effect on non-target biosymbiotic microorganisms is evaluated. For example, the diversity of non-target
organism symbiotic microbial communities on the gene edited wheat and the
receptor wheat has no
significant difference, and the gene edited wheat has no significant influence on the diversity of microorganisms in non-target organisms. The scientific evaluation method is established, the influence of the gene editing crops on the non-target biosymbiotic microorganisms can be more comprehensively and accurately analyzed, and the method has important significance for improving
environmental safety evaluation of the gene editing plants.